HATCHERY Feed & Management Vol 8 Issue 4 2020

Vol 8 Issue 4 2020

BUYER’S GUIDE & DIRECTORY Bluefin Tuna Hatchery Developments Vitamin Supplementation in Marine Larvae Published by: Aquafeed.com LLC. Kailua, Hawaii 96734, USA www.aquafeed.com info@aquafeed.com

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Hatchery Feed & Management Vol 8 Issue 4 2020

HATCHERY FEED & MANAGEMENT

Hatchery Feed & Management magazine is published by Aquafeed.com LLC. Kailua, Hawaii 96734, USA. www.aquafeed.com Hatcheryfm.com

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VOL 8 ISSUE 4 2020

Contents 4

News Review

Skretting’s new marine broodstock diet responding to hatchery needs.

Aller Aqua upgrades fry feeds for faster growth and lower mortality.

Hendrix Genetics to build shrimp broodstock center in Indonesia.

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Spring Genetics inks deal with Africa’s largest integrated tilapia producer.

Benchmark Genetics’ agreements with land-based facilities. Largest Brazilian tilapia fry producer rebrands as AquaGenetics do Brasil.

Atlantium Technologies partners with PR Aqua.

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8

Bluefin tuna hatchery success: A first step in resurrecting

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Interview with Henrik Aarestrup

San Diego, California as the “Tuna Capital of the World”

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Dietary B vitamins supplementation in microdiets for marine fish larvae: Can it bring advantages to performance and bone malformations?

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BlueMarine³.Com project studies hatchery techniques

for seaweed, bivalves and shrimp in support of Flemish

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aquaculture development

22

Buyer’s guide

49

Calendar of Events

Columns

39

15 Greg Lutz – When less is more:

circular and cyclical mating systems

Hatchery Feed & Management Vol 8 Issue 4 2020

4

NEWS REVIEW

HATCHERY FEED & MANAGEMENT

Highlights of recent news from Hatcheryfm.com News as it happens in the Newsroom at Hatcheryfm.com - sign up for our free newsletter for biweekly updates

Skretting’s new marine broodstock diet responding to hatchery needs Skretting will globally introduce Vitalis PRIMA in the coming months, a new marine broodstock diet that supports the health of parent fish and young fry, thereby improving the performance of hatchery systems. Vitalis PRIMA, which will replace Vitalis CAL, incorporates some of the latest technology from ground-breaking dietary research as well as commercial input from broodstock and hatchery managers. It is the latest solution to come from over 25 years of broodstock feed development at Skretting.

Aller Aqua upgrades fry feeds for faster growth and lower mortality Extensive studies with trout fry at Aller Aqua Research and at a farm test showed improved growth when fish were fed ALLER INFA EX GR and ALLER FUTURA EX GR than fish fed with major competing brands. At the same time, fish fed with Aller Aqua fry feeds were more uniformly sized, showed no deformities and

had lowered mortality down to one percent in trout fry up to 3 g.

“We consider this another milestone in the evolution of fry feeds at Aller Aqua in recent years, focusing on the needs of the fish and benefits for the farmer. The upgrade applies to all of Aller Aqua’s fry feeds helping all of our fry feed customers to improved performance,” the company said.

Hendrix Genetics to build shrimp broodstock center in Indonesia Hendrix Genetics will establish a shrimp broodstock multiplication center in Indonesia.

Beginning early 2021, Indonesian shrimp hatcheries will be able to source high-quality, specific pathogen-free (SPF) broodstock, locally reared.

The initial operations of the new company, PT Kona Bay Indonesia, will be based at an SPF facility in West Java. Within two years, Hendrix Genetics plans to move to brand new state-of-the-art SPF facilities in North Bali, capable of producing over 100,000 SPF vannamei broodstock per year.

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Spring Genetics inks deal with Africa’s largest integrated tilapia producer Spring Genetics, Benchmark’s tilapia genetics business, signed a five-year broodstock agreement with Africa’s leading tilapia producer, Lake Harvest Group.

The agreement secures Lake Harvest exclusive supply of high performing Nile tilapia broodstock from Spring Genetics’ advanced selection program in the USA to its commercial operations in Zambia and Zimbabwe and a license to produce and sell sexreversed fingerlings to third party farms in this region.

The company also signed new agreements with Proceal and Piscícola Botero to supply tilapia broodstock in Colombia.

Benchmark Genetics’ agreements with land-based facilities Benchmark Genetics and AquaCon signed an agreement to supply ova to the land-based facilities that AquaCon will build in Maryland, USA. Benchmark’s Icelandic company, StofnFiskur, will supply approximately 40 million ova to AquaCon over five years.

Benchmark Genetics Norway and Salmon Evolution also entered into an agreement on collaboration on

Largest Brazilian tilapia fry producer rebrands as AquaGenetics do Brasil

Piscicultura Aquabel, the largest Brazilian producer of tilapia fry and juvenile, is now known as AquaGenetics do Brasil Ltda. As part of the corporate restructuring after the recent

acquisition of AquaAmerica, AquaGenetics do Brasil becomes the exclusive distributor of the genetics brands of Aquabel and AquaAmerica tilapia in the Brazilian market. Aquabel started the production of tilapia fry in 1994 and in 2016, the German EW Group acquired a majority stake in the company, which now is part of its tilapia genetics and distribution business, GenoMar Genetics Group.

genetics, ova, product deliveries and expertise development related to salmon farming on land. The sevenyears agreement, lasting from 2020 to 2027, makes Benchmark Genetics the main supplier of ova to Salmon Evolution during this period. The contract also includes collaboration on research and development projects for breeding and landbased aquaculture.

Atlantium Technologies partners with PR Aqua PR Aqua was appointed as the new distributor in Canada for Atlantium’s Hydro-OpticTM (HOD) ultraviolet (UV) technology in the aquaculture market segment. PR Aqua will also provide Atlantium HOD UV into aquaculture projects they design in the United States.

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Henrik Aarestrup is Vice President for Latin America & Hatchery and Shrimp of BioMar Group, a global aquafeed company, headquartered in Denmark. BioMar feed types cover the full life cycle of aquaculture species, meeting the nutritional needs for healthy growth from larval stage to juvenile, on-growing and broodstock. BioMar produces hatchery diets for fish and shrimp, with sales in more than 80 countries.

Henrik Aarestrup has been with BioMar since 2007, first as International Marketing Manager, then as Global Marketing Director, and as BioMar Group Vice President Emerging Markets Division, leading operations in China and other Asian Markets as well as Central and South America.

INTERVIEW HATCHERYfm: Would you tell us about your background and what attracted you to aquaculture?

HA: I have worked in international management for nearly 25 years starting in another well-known Danish company, LEGO, but for the last 14 years aquaculture has been my home. It is an industry that is characterized by a lot of entrepreneurship, a continuous striving for improvement as much is still to be learned. I am amazed by the very cordial tone between suppliers, customers and not least the research community. This makes it a nice business to be in. Moreover, we also contribute to an overall purpose which is to provide healthy and more sustainable food to the world, and at a personal level this is very important to me.

HATCHERYfm: BioMar’s hatchery range has been growing exponentially in recent years.

The company has made strong investments in the main production site for BioMar hatchery products at the BioMar factory in France and in its Aquaculture Technology Centers around the world. Is there a specific goal in terms of production, geographic reach or product development?

HA: We have in the past decade achieved a very solid position in the Mediterranean marine hatchery segment

Hatchery Feed & Management Vol 8 Issue 4 2020

with Henrik Aarestrup and we currently use this base to expand to other marine species in both Americas and Asia, e. g. we just finalized very successful hatchery trials with barramundi in Vietnam and with red snapper in Central America. At the same time, we have expanded in the shrimp hatchery and nursery segment. Actually, we have sold hatchery feeds to shrimp since 2008, but only in the last six years we have started to expand in the shrimp grow out.

Now we are in a much better position to target shrimp hatchery markets worldwide. We combine local and global feed ranges and have our own presence in some of the key markets like China, Ecuador, Vietnam and Mexico, in addition to strong distributors in other important markets.

HATCHERYfm: BioMar’s diets for marine fish and shrimp hatcheries seem to have a good reputation for their performances. Can you explain some of the key features that have led to this success?

HA: One of the breakthroughs which led to the current success was the addition of probiotics in both larval and nursery diets. BioMar, being the first and still the only with a probiotic approved by the European Commission for a documented effect on reducing

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mortality and deformities obviously helped to establish the success. Another important factor is that we offer a product range that works extremely well across species, even in those that could have specific requirements.

Strong R&D efforts and investments have been done over the past few years and our knowledge in hatchery nutrition has increased dramatically.

This is strongly reflected in the formulation of the diets, from broodstock, to larvae, post-larvae and fry.

Also, we approach hatchery feeds in a much broader and complete way. A feed is much more than its nutritional composition, especially with larval diets.

The physical characteristics, the way it behaves in the water, its attractability and its stability in water, are as important. And the way that the feeds are being used needs to be adapted to the unique characteristics of each species and each hatchery. We strongly believe that working together with hatcheries is fundamental for the success of our feeds and ultimately for the success of the hatcheries.

HATCHERYfm: With the intended partnership with Viet-Uc and other Asian investments, BioMar is ready to make a big move into the Asian shrimp markets. What are the main challenges you think these markets face and what is BioMar doing to support shrimp PLs production?

HA: Disease prevention is one of the key challenges for Asian shrimp markets and that has to start from the earliest life stages. We have now invested more than 15 years of work in the area of probiotic application in feed, in partnership with Lallemand Animal Nutrition, and we will continue developing in this area and have a number of exciting projects going on. But we are also looking into different types of solutions. Having spent a large share of my time in the last four years in Southeast Asia – mainly China – taught me that there are too many “hocus pocus” products on the market – undocumented products which claim to solve almost everything. For us, it is important that we back up our products with proper documentation giving real solutions for the farmers.

HATCHERYfm: RAS farming is an emerging segment within the aquaculture industry and BioMar has

been developing the next generation of RAS feeds.

How does this feed work and what are the main advantages compared to standard hatchery diets?

HA: In RAS systems, the physical aspects of the feed and nutritional optimization is even more important than in standard systems. To put it simply: everything which is not turned into growth pollutes the system. Dust and fines must obviously be absent from any RAS feed. But at the next level, as unused nutrients will be passed to the tank environment over the gills or in the form of feces, the nutrient balance needs to be perfectly optimized to fit the need of the fish. And finally, what does come out in the form of feces must be easy to remove to keep an optimal water quality.

BioMar has been heavily involved in the development of the RAS feeds for the on-growing stage of fish, both in freshwater and in seawater. We have now used all this knowledge and transferred it to marine nursery feeds. These new generation RAS feeds assure the best efficient use of the nutrients for growth and health status and promote a better structure of the feces allowing a simpler removal of solids from the RAS systems. This is just the tip of the iceberg since it will then have an impact on the stabilization of the systems, biofilter capacity, improved skimmers function, less potential issues with hydrogen sulfide, improved water quality, etc. at the end of the day, reduce production costs and increase performance.

HATCHERYfm: BioMar scaled up its fry and RAS facility in Denmark, which is expected to produce premium range of fry feeds. Can you tell us how the fry range is performing?

HA: The new production line in Denmark has proven to deliver improved physical quality of the pellets and the option of introducing an even smaller range of extruded mini pellets. The feedback from our customers so far has been very positive and benchmark trials are looking good.

HATCHERYfm: What do you think is the next big thing in hatchery developments?

HA: In marine hatchery, everybody is looking for the holy grail which is life feed substitution. We have promising results that move the boundaries with very early co-feeding, but it is an area that requires a heavy effort in R&D.

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Bluefin tuna hatchery success: A first step in resurrecting San Diego, California as the “Tuna Capital of the World” Mariana Michelato, Laura Martinez and Alejandro Buentello, Sr., Ichthus Unlimited San Diego, CA, once was known as the “Tuna Capital of the World” (Ellis, 2008). Following the first tuna cannery startup (1911), the city became a world leader in commerce associated with tuna fisheries.

By the 1960s, San Diego’s third-largest economic enterprise would be tuna, superseded only by the U.S. Navy and aerospace, employing over 40,000 San Diegans. After years of overfishing and the tuna embargo, this commerce has all but disappeared from Southern California.

In 2016, the National Marine Fisheries Service was petitioned to have the Pacific bluefin tuna, Thunnus orientalis (PBFT), listed under the Endangered Species Act. Assessment by the 2016 International Scientific Committee for Tuna and Tuna-like Species indicated that the stock was “alarmingly overfished”, yet still undergoing high rates of exploitation (Craig et al., 2016). At that time, the Inter American Tropical Tuna Commission also adopted the strictest PBFT conservation measures in history for the Eastern Pacific Ocean (EPO, IATTC, 2016). These actions

combined emphasize the dire situation of the PBFT – and the need for restoration research.

Reactive regulatory measures alone have limited capacity to protect endangered populations of fish.

While science provides suitable fishery restrictions, implementing these is extremely difficult. The PBFT stock is harvested by several countries and over 80% of the capture occurs in the Western Pacific Ocean (WPO). When forage fish resources at WPO become overexploited, a hunger-driven trans-Pacific PBFT migration is triggered to the EPO (Matsukawa, 2006). Japan’s use of large amounts of wild-caught juvenile PBFT to stock tuna ranches adds to the overall fishing pressure and management complexity.

Since tuna ranching started, there has been an exponential increase in the volume of baitfish used to feed tuna in net pens worldwide (WWF, 2005).

This is the case in northwest Mexico where most purse seine sardine catches are destined for tuna ranching operations (del Moral et al., 2010). A new assessment delivered to the Pacific Fishery Management Council

Figure 1. Snout hemorrhage after collision (a) and operculum malformations (b and c) are common issues affecting survival in bluefin tuna larviculture.

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successful with after-shipment survival exceeding 80%. Importantly, only species endemic to a particular area will be cultured using IU fish in that area.

For example, PBFT in the U.S. West Coast and ABFT in the Gulf of Mexico and the U.S. East Coast.

Figure 2. Cannibalism (a) may be controlled with regular sorting and grading (b) of tuna larvae.

(NOAA, 2020), indicated that the Pacific sardine (Sardinops sagax) stock – a fishery resource shared

by Canada, the United States and Mexico – remains at extremely low biomass. In the United States and Canada, the commercial fishing for Pacific sardines is prohibited because the population is below precautionary levels.

Ichthus Unlimited (IU) is a small and diverse company founded in 2015 by American entrepreneurs. Its interdisciplinary research team is at the forefront of tuna research and believes a proactive strategy may work best in resolving these important and challenging problems. With a three-pronged approach, based on controlled reproduction and larviculture of PBFT, sustainable tuna feeds (Buentello and Albertson, 2018) and novel marine cage technology, IU aims at bringing bluefin tuna back to the “Tuna Capital of the World”. Here we share the recent success of tuna hatchery research and, as of the submission of this article, bluefin tuna juveniles > 140 days post hatch (dph) are thriving at IU’s hatchery in San Diego.

The generous funding support from the Foundation for Food and Agriculture Research, the Illinois Soybean Association, The Ohio Soybean Promotion Council, the Minnesota Soybean Research & Promotion Council, and the USDA-NIFA-Small Business Innovation Research Program is kindly acknowledged.

Egg sourcing and global logistics Although the target species for IU’s San Diego hatchery is the PBFT, this technology can be applied to other scombrid species. Therefore, a complex network of international suppliers of fertile eggs of PBFT, Atlantic bluefin tuna (ABFT, T. thynnus) and yellowfin tuna (T. albacares) has been established to improve global shipment and husbandry protocols. Even during the COVID-19 pandemic, which has brought extremely challenging freight conditions, these protocols proved

International and domestic live feed procurement network After tuna eggs hatch and consume the yolk, the first feed tuna larvae are composed of small live zooplankton and other fish larvae from a different species, until they can be weaned to an artificial diet. A unique group of cooperating institutions and vendors was formed including domestic collaborators in California, Texas, Mississippi and South Carolina and international partnerships in Japan and Norway. Just in time, logistics were executed such that timely delivery of organisms of the right developmental stage were made available to serve as larval tuna feed.

Preliminary research on weaning diets In collaboration with the Spanish Institute of Oceanography (IEO), Texas A&M University, Scripps Institute of Oceanography (SIO) and other academic institutions, IU has been conducting targeted research to optimize weaning diets for larval tuna and juveniles.

Existing live feeds and artificial feeding protocols for larval and juvenile tuna species are associated with poor survival, growth and stress. Low swim bladder inflation rates, surface and sinking deaths, dispersed sizes, malformations and tank wall collisions are common issues (Buentello et al., 2016a). Mortality observed during the first stages of life is partly due to nutritional deficiencies (de La Gandara et al., 2010; Partridge, 2013; Buentello et al., 2016).

A preliminary trial comparing commercial (Magokoro, Sujico and Sparos) vs IU diets over a 4-week feeding period resulted in IU diets supporting a significantly (P < 0.05) higher survival (55%) compared to the other test diets (< 35%). Other studies have shown a wide range in weight gain variation with no significant differences among fish fed various experimental diets.

Because growth and survival are the two most relevant performance indicators in larviculture of fish, these results demonstrate that IU diets can deliver equal performance and superior survival under standardized rearing conditions.

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Figure 3. Bluefin tuna 80 days post-hatching (~111 g and 22 cm total length).

Problems and perspectives Preparation for the 2020 season started Q1 2020 with a first shipment received on July 11, approximately 30 hours after fertilization. Upon arrival, water parameters in the egg container were 19°C, 37 ppt and 150% saturation for water temperature, salinity and dissolved oxygen, respectively. After acclimation to hatchery conditions, eggs were stocked at a density of 18 eggs/L in 10, 500-L round tanks. Throughout the rearing period, average water quality parameters have been maintained at 25.6°C, 160% and 33.2 ppt for water temperature, dissolved oxygen saturation and salinity, respectively. Flow rates were progressively increased from 0.00007 to 0.5 L/min from week 1 to 13. At this point, fish were transferred to larger systems at NOAA’s Southwest Fisheries Science Center (flow-through) or to IU’s 32,000-L closed recirculating system. Photoperiod was regulated such that 24 hours of light were provided during the first 10 dph followed by a natural photoperiod until 25 dph. Thereafter, light irradiation was regulated in a diel manner, with intensity adjustments for day and night time. Survival among tanks was highly variable with average values ranging between 1.0 and 0.1% 60 dph, which is lower than that reported for bluefin tuna eggs which were not subjected to trans-oceanic shipping (Satoh et al., 2013).

Survival issues arose in part from shipping temperatures which may have resulted in malformations (Fig. 1) as reported for other scombrid species (Wexler et al., 2011; Guillen et al., 2014) and/or nutritional deficiencies (Fraser & de Nys, 2005; Cobcroft & Battaglene, 2013) which affect larval stages in various marine fish when biological organogenesis and morphogenesis processes occur (Mourente & Toucher, 2009; Buentello et al., 2016b). These problems may result in reduced biological performance (e.g., diminished growth rates and survival) and in the production of unmarketable fish, but can be addressed through management and nutritional interventions.

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Tank collisions (Fig. 1) were controlled with appropriate illumination as well as limiting personnel access to rearing tanks; whereas, cannibalism (Fig. 2) was controlled by maintaining a suitable amount of prey items at all times as well as semi-continuous sorting and size-grading of fish.

Future research Although scombrids are extremely prolific, multiple batch spawners (Schaefer, 2001), research underlying causes of juvenile tuna mortality is still very much needed. IU’s first attempts at larviculture met with relatively low survival rates. Additional research is required to determine the potential impact of the nutritional quality of live feeds, and other factors linked to diminished survival. However, it is reasonable to expect in the near future that PBFT fertile eggs originating from IU oceanic cages near San Diego (~15 miles SW) will result in significantly increased survival rates.

The ontogeny of endothermy, as well as vision development, appears as a highly profitable area in tuna research with practical applications in larviculture.

To hunt for prey in deep and cold water, some tunas can maintain a warm body temperature that supports their central nervous system, key organs and eyes.

The visual ability supported by a heated retina in deep cold waters makes these fish efficient predators (Fritsches et al., 2005). Studies on the ontogeny of endothermy are been jointly pursued by researchers at California State University Fullerton and IU, although studies on vision development in juvenile tunas are presently limited, in collaboration with SIO researchers, IU plans to address this topic as it affects a period in the life of tuna when significant mortalities still occur.

Conclusion and perspectives Current methods in tuna ranching based on wild-captured fish for the stocking of net pens and the feeding practices using baitfish severely limit its sustainability and full potential, running contrary to best management practices and environmental responsibility.

For tuna aquaculture to become sustainable, various factors must be controlled. At minimum a) suitable environmental conditions need to be provided [in ascending order, for survival, growth and reproduction],

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b) a reliable source of hatchery-raised juveniles for net pen stocking, c) a complete, balanced feed must be commercially available for all developmental stages and â&#x20AC;&#x201C; becoming more relevant every day â&#x20AC;&#x201C; d) control over waste discharges, e) disease prevention and management and, not least, f) have economic feasibility.

Today, the tuna ranching industry is arguably the highest value form of aquaculture in the world and the associated burgeoning of support industries and economic multipliers may have a significant commercial impact and societal benefit to coastal areas in southern California. Therefore, the re-establishment of a tunarelated industry in San Diego makes sense if we can provide hitherto unavailable technologies that carefully balance the sustainability of resources, the carrying capacity of the marine ecosystem and the economic development needed as impetus.

Today, IU offers science-based practical solutions to all factors listed above. Execution of these solutions and the shift from R&D to commercial depends on a successful capital raise with attractive returns for investors, which is something IU is currently pursuing.

References available on request

More information: Mariana Michelato Project Manager Ichthus Unlimited, USA

LEARN CONNECT INSPIRE

WEBINAR SERIES

Save the dates!

Disease prevention and management March 22, 2021

Breeding and genetics April 26, 2021

Laura MartĂ­nez Hatchery Manager Ichthus Unlimited, USA

Feed for hatcheries May 17, 2021

Water conditioning and probiotics June 14, 2021

Alejandro Buentello, Sr. President Ichthus Unlimited, USA E: abuentello@ichthusunlimited.com

AquacultureConferences.com

Hatchery Feed & Management Vol 8 Issue 4 2020

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Dietary B vitamins supplementation in microdiets for marine fish larvae: Can it bring advantages to performance and bone malformations?

Michael Viegas, Jorge Dias, Luís Conceição, SPAROS, Paulo Gavaia, Vincent Laizé, Center of Marine Sciences of Algarve (CCMAR), Universidade do Algarve

Figure 1. Examples of skeletal malformations in Senegalese sole (Solea senegalensis) post-larvae (74 DAH).

The production output and profitability of the aquaculture industry is highly dependent on the rearing of healthy, fast-growing fish larvae and juveniles.

Still, marine hatcheries face challenges that are often associated with variable results during larval and juvenile rearing, due to problems such as stress and disease susceptibility, size dispersion, sub-optimal growth and high incidences of skeletal malformations.

Skeletal malformations (Fig. 1) are a major concern for marine hatcheries since most skeletal anomalies have their onset during bone development in early larval stages and can affect swimming competence and the

Hatchery Feed & Management Vol 8 Issue 4 2020

capability to compete for food. This reduces growth rates, increases mortality and significantly affects animal welfare. All together, these issues can result in relevant biological and economical losses.

A dietary imbalance of certain micronutrients, such as vitamins, is known to impact fish larval ossification, consequently leading to an increase of skeletal anomalies and/or an imbalance in calcification. Also, dietary B-vitamins requirements have been suggested to be higher at larval stages when compared to juvenile and adult stages. Analysis of vitamins in fish eggs and natural live prey supports this assumption. Given the

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Figure 2. Effect of B-vitamin supplementation to Sparus aurata bone-derived cell cultures. (A) Micrographs of non-mineralized and mineralized VSa13 bone-derived cells from Sparus aurata (Calcium deposits are stained red with calcium specific alizarin red-S). Bar represents 50 Âľm. (B) Graphical representation of extracellular matrix (ECM) mineralization increases due to B-vitamin supplementation.

high sensitivity of fish larvae to potential nutritional deficiencies, larval microdiets should contain safe and surplus vitamin levels to compensate for potential challenging biological and technical conditions.

Before performing a nutritional feeding trial, we must consider that bone development is completely dependent on a set of specific cellular processes such as proliferation and mineralization that are known to be affected by vitamins in a dose-dependent manner. In vitro systems have been used for over 25 years to assess the effect of dietary micronutrients on such cellular processes. To better understand the action of B vitamins on fish bone cytotoxicity and mineralization, we supplemented an in vitro cell system developed from the vertebra of gilthead seabream Sparus aurata (Fig. 2), with thiamine (vitamin B1) and pyridoxine (vitamin B6) and performed proliferation and mineralization assays.

The proliferative/cytotoxic effects of water-soluble B vitamins were shown to be dose-dependent and cytotoxic effects were only observed at very high doses.

Both vitamins enhanced extracellular matrix (ECM) mineralization (Fig. 2).

Taking into consideration these in vitro results, a study was undertaken with Senegalese sole post-larvae to evaluate the dietary supplementation of three B

vitamins (thiamine, pyridoxin and cyanocobalamin), individually (B1, B6 and B12) and in combination (Full).

After being fed with the experimental diets from 43 to 74 DAH, supplemental dietary vitamins had little impact on the survival and growth performance of sole larvae.

Regarding bone status, several parameters were evaluated. A dual bone staining methodology, used to assess the mineral apposition rate (MAR) in vertebrae, showed that none of the vitamin supplementation strategies had a significant effect on MAR (Fig. 3). By way of X-ray imaging analysis, the relative bone mineral content (bone density) and the degree of severity of malformations in different areas of the vertebral column (using a score ranging from 0: no malformations to 3: presence of commercially severe malformations) were also shown to not be affected by the dietary modifications (Fig. 3).

The overall results of this study show that although thiamine (Vitamin B1) and pyridoxin (Vitamin B6) boosted cell-mediated mineralization in a seabream bone-derived cell culture, further supplementing the current commercial larval feed (WINFlatÂŽ) with thiamine, pyridoxine and cyanocobalamin vitamins brings no clear benefits on enhancing the growth performance and bone status of Senegalese sole post-larvae.

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Perspectives The role of B vitamins in the modulation of immune competence is attracting interest in humans. Therefore, its use â&#x20AC;&#x153;beyond requirement levelsâ&#x20AC;? to reinforce the immune status is an area worth exploring in fish larvae.

The generation of new knowledge on the optimal nutrition at the fish larval stage by assessing the several biologic criteria, e.g., growth, skeletal anomalies, stress and disease resistance, is key to the continuous improvement of high-quality commercial microdiets for marine fish larvae.

More information:

Figure 3. Effect of B-vitamin dietary supplementation to Senegalese sole post-larvae on (A) vertebral malformation scoring, (B) vertebral mineral apposition and (C) bone density.

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Hatchery Feed & Management Vol 8 Issue 4 2020

Michael Viegas Researcher SPAROS Lda, Portugal E: michaelviegas@sparos.pt

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Lalsea Biorem is a mix of bacteria specific for improving the pond environment through the reduction of organic material and nitrogen compounds.

COLUMN

Trends in breeding and genetics C. Greg Lutz, Ph.D. Dr. Greg Lutz is a Professor with The Louisiana State University Agricultural Center.

He is also an author and consultant. E: lutzaqua@att.net

When less is more: circular and cyclical mating systems As we saw in my last column, the “effective” number of animals reproducing in many hatcheries is usually significantly less than the actual number. However, there are strategies available to make the most of the broodstock you have.

One objective in most aquaculture breeding programs is to maintain as much genetic variation as possible from one generation to the next by avoiding mating of closely related individuals. This is especially true for conservation aquaculture when wild populations

are to be enhanced with hatchery-reared animals.

In these situations, the space available for holding broodstock is usually not as important a consideration as are the numbers of breeding individuals available for spawning. If the number of potential breeding animals is more than the available hatchery space can accommodate, then broodstock can be selected at random. Unfortunately, if you are practicing this or any other form of selection, in every successive generation you are conserving some desirable (or adaptable,

Table 1. Percent increases over NE with various mating systems.

Comparable Population Numbers and Percent Increases over NE Mating Strategy

Males Females Total Effective

NM

NF N NE

No. Groups Circular

24 48 72 64

Falconer

HAN-R

Cockerham’s

4 70.08 109.5% 69.64 108.8% 69.56 108.7% 69.53 108.6%

24

48 72

64

6

75.53 118.0% 73.56 114.9% 73.58 115.0% 73.54 114.9%

24

48 72

64

8

82.44 128.8% 77.45 121.0% 77.14 120.5% 77.14 120.5%

24

48 72

64

12

100.83 157.5% 85.55 133.7% 85.46 133.5% 85.27 133.2%

96

192 288 256

4

262.02 102.4% 261.6 102.2% 261.52 102.2% 261.52 102.2%

96

192 288 256

8

274.13 107.1% 269.35 105.2% 269.04 105.1% 269.04 105.1%

96

192 288 256

16

315.34 123.2% 285.41 111.5% 284.59 111.2% 284.59 111.2%

96

192 288 256

32

472.13 184.4% 318.12 124.3% 316.17 123.5% 316.17 123.5%

192

384 576 512

4

518.01 101.2% 517.59 101.1% 517.51 101.1% 517.51 101.1%

192

384 576 512

8

530.07 103.5% 525.33 102.6% 521.02 101.8% 521.02 101.8%

192

384 576 512

16

570.73 111.5% 541.36 105.7% 540.55 105.6% 540.55 105.6%

192

384 576 512

32

722.87 141.2% 574.03 112.1% 572.10 111.7% 572.1 111.7%

Hatchery Feed & Management Vol 8 Issue 4 2020

16

Circular, 4-Group Mating

Cockerham’s Cyclical System, 5-Group Design

Same transfer pattern each generation

Generations 1,3,5, etc

Generations 2,4,6, etc

Falconer’s Cyclical System, 4-Group Design

Generations 1,4,7, etc

Generations 2,5,8, etc

Generations 3,6,9, etc

HAN-Rotational System, 4-Group Design

Generations 1,3,5, etc

Generations 2,4,6, etc

HAN-Rotational System, 5-Group Design

Generations 1,5,9, etc

Generations 2,6,10, etc

Hatchery Feed & Management Vol 8 Issue 4 2020

Generations 3,7,11, etc

Generations 4,8,12, etc

17

or random) portion of the genetic variation in the population and often discarding the rest.

Mathematical theories behind these broad generalizations have been firmly established over the past century. It has become commonplace during the past two decades for some aquaculture “experts” to claim they can employ mating systems that eliminate inbreeding. However, none of these systems can actually accomplish that goal in a closed population, and their advantages are generally short-term. As the quantitative geneticists Kimura and Crow stated in their 1963 article On the maximum avoidance of inbreeding, “a system that avoids mating of relatives for as long as possible does so at the expense of a more rapid final approach to homozygosis.” And, it does so while sacrificing potentially valuable genetic improvement, including improved fitness.

While conserving all of the genetic variation available within a closed population is impossible in most situations, some strategies are available to minimize these losses. And when applied correctly, the result is a mathematical increase in the effective number of reproducing individuals (Table 1).

Many people confuse the relationship between levels of heterozygosity and levels of inbreeding,

but things are usually more complicated in a hatchery setting. Inbreeding by itself only changes the portion of heterozygotes without directly changing gene frequencies, such that heterozygotes become rarer while homozygotes become more frequent.

But here’s the rub: if one homozygote or the other is inherently less fit, gene frequencies will still change over time. As inbreeding increases the levels of homozygosity in a population, inbreeding depression can require moderate to high levels of selection to maintain adequate numbers of broodstock from one generation to the next, with unintentional or unforeseen loss of variation.

Some mating systems tend to minimize the accumulation of inbreeding after many generations, but they also result in a more rapid accumulation during the early generations. As a result, the rate of decrease in heterozygosity in a closed population may not have a large impact in terms of which mating system is the most practical in the long term for many aquaculture breeding programs. But since inbreeding can only accumulate over time, inbreeding levels in the earlier generations are disproportionately concerning in closed populations that may not be maintained for more than 20 or 30 generations.

Hatchery Feed & Management Vol 8 Issue 4 2020

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Most of the mating systems that have been developed for minimizing inbreeding in small populations involve subdividing the population into a number of groups, and then maintaining distinct breeding groups in every following generation. Perhaps the simplest of these systems is referred to as circular mating. In this system, individuals within each group are allowed to mate and reproduce, and in the subsequent generation, the male offspring from each group are transferred to mate with female offspring from an “adjacent” group, in a circular rotational pattern (Fig. 1, 2). When broodstock numbers are low, inbreeding accumulation rates are somewhat high in earlier generations of circular mating. But they tend to level off over time and actually remain at levels somewhat lower than those attained by other mating systems. Conservation programs for rare livestock breeds have often used this system in the past.

Cyclical mating systems also rely on exchanging males between groups but the exchange patterns vary, cyclically, over generations (Fig. 1, 2). As a result, the accumulation of inbreeding is also cyclical over time.

Some of these systems have been proposed by and named after legendary quantitative geneticists, such as Falconer’s system, Falconer’s maximum avoidance system, Wright’s maximum avoidance system and Cockerham’s system. In cyclical mating systems, inbreeding levels among groups can bounce up and down from generation to generation, but the longterm trend still results in accumulation. That, of course, is an inescapable truth. An interesting summary of some cyclical systems is presented by Nomura and Yonezawa (1996).

Falconer’s cyclical system exhibits a large initial increase in inbreeding, followed by negative values, and this cycle repeats itself with increases outgaining losses in each cycle. The HAN-rotational system exhibits a cycle of large increases followed by significant reductions and then moderate increases, also with a more or less linear accumulation when averaged over generations. Cockerham’s mating system also exhibits cyclical patterns of larger and smaller increases with generations of virtually no change at the end of each cycle.

So… what did Nomura and Yonezawa conclude in their analysis of these mating systems? Cyclical systems were better at minimizing inbreeding levels in earlier generations. And, if you maintain an even number

Hatchery Feed & Management Vol 8 Issue 4 2020

of groups, Cockerham’s system seems to be the best choice, while the HAN-rotational system generates the best results if you are maintaining an odd number of breeding groups.

In the circular mating scheme, an increase in the number of groups can actually result in increased inbreeding. When more than six groups are used in a circular breeding program, the inbreeding coefficient per generation is actually greater than what would be expected from random mating (Fig. 1). The underlying reason is an increase in mating between cousins. Females in any given group are mated with males coming from the same “other” group in every generation, and so the probability of single cousin mating is [ (the number of groups)(total number of males + total number of females)-(number of groups)2 ] / (total number of males * total number of females).

Cyclical group mating systems really do provide for a somewhat reduced accumulation of inbreeding, but as the effective population size reaches 250 or higher, the advantage is negligible and infrastructure and labor requirements become important factors to consider. Nonetheless, in some of these situations, it still makes sense to maintain distinct groups of breeding stock within each generation. For conservation aquaculture activities it may be desirable to maintain endangered species in smaller numbers at a variety of locations. Similarly, if a number of small producers establish a coordinated effort for a group mating system, they can collectively enjoy a much greater effective population size and the reduced accumulation of inbreeding that results.

These systems have been developed with mathematical models to minimize the loss of genetic variation within closed populations. They should not be incorporated haphazardly in selection programs because the math changes when selection is imposed from one generation to the next. Caveat emptor.

19

BlueMarine³.Com project studies hatchery techniques for seaweed, bivalves and shrimp in support of Flemish aquaculture development Mathieu Wille, Vincent Vermeylen, Jessica Knoop, Ghent University, Luc Roef, Proviron, João Dantas Lima, IMAQUA

Figure 1. Photobioreactors at Proviron.

Growing interest to develop local aquaculture production in Belgium is met with initiatives that range from indoor penaeid shrimp farming to bivalve and seaweed culture in the offshore windfarms and the restoration of native flat oyster (Ostrea edulis) reefs in the North Sea. These local aquaculture initiatives are, however, commonly struggling to source sufficient, qualitative starting material in terms of genetic background, disease status, size requirements, etc.

While Belgium does not have a strong tradition in

marine aquaculture production, it has both renowned academic institutes, as well as several important industry players with extensive expertise throughout the aquaculture supply chain. In the BlueMarine³.Com project, six Flemish companies and three research groups from Ghent University jointly tackle the challenges to adapt existing hatchery protocols for seaweed, bivalves and (penaeid) shrimp to the local context, making them more environmentally friendly and economically feasible. The developed techniques

Hatchery Feed & Management Vol 8 Issue 4 2020

20

Figure 2. Survival to postlarva stage of larvae fed two different microalgae species in the live or freeze-dried form. *denotes statistically significant difference between the live and freeze-dried form.

may however prove valuable for hatcheries worldwide.

In view of the high costs for land, energy and labor in Europe, BlueMarine³.Com aims to develop compact closed systems with a high degree of automation.

In addition, the idea is to study and value synergies between hatchery techniques for the different species groups in terms of infrastructure, resources, breeding and management in a so-called “multi-species hatchery approach.” The ultimate goal is to set up a (multispecies) hatchery pilot installation that can stimulate further innovation and develop more large-scale hatchery operations.

Seaweed strain selection The Laboratory of Phycology (UGent), in collaboration with SIOEN, which develops bio-based and biodegradable textiles as seaweed substrate, is working on a strain collection of commercial seaweeds.

The collection has since been expanded with local strains of Saccharina, Undaria, Porphyra and Ulva from the southern bight of the North Sea. In addition, work is also being done on the life cycle management of commercial seaweed species. For example, for Undaria a gametophyte strain was successfully isolated.

Male and female gametophytes appear to be very fertile. The optimal nutrients, temperature and light

Hatchery Feed & Management Vol 8 Issue 4 2020

conditions for growth and fertility are now being determined. For Ulva, a genetic map for commercially interesting performance traits is being developed.

Microalgal replacement for shrimp early stages For shrimp (Litopenaeus vannamei) hatchery techniques, the Laboratory of Aquaculture & Artemia Reference Center (UGent), IMAQUA (a spin-off of UGent) and Proviron (microalgae technology and production company) joined forces. Shrimp farms in Europe, which are becoming increasingly popular, largely rely on the import of post larvae from a handful of hatcheries in the U.S., with complex logistics and unreliable supply in terms of quantity and quality as a result. The current project, therefore, investigates the feasibility of local hatchery production. Broodstock rearing and reproduction are done at the facilities of IMAQUA in closed bio-secure systems. Subsequent larval rearing trials are then performed at the Laboratory of Aquaculture & ARC in 100-L tanks. In view of the limited availability of good quality seawater in Belgium and to minimize environmental impacts, hatchery trials are performed in a recirculating aquaculture system (RAS). Since inhouse microalgae production is a large cost for hatcheries, especially small ones, in terms of facilities and labor (Oostlander et al.

21

will now finetune the recirculation rate in the RAS, further optimize dry algae-based diets and implement automatic feeding protocols of these diets to further improve the production process.

Figure 3. Shrimp broodstock system at IMAQUA.

Figure 4. Shrimp breeders at IMAQUA.

2020), and moreover is not always reliable, research investigated the replacement of live algae with “off the shelf” freeze-dried algae products of Proviron.

Consistently good survival (50-60% at post-larval stage) is obtained with two diets consisting of freeze-dried algae only, which was similar or only slightly lower compared to feeding live algae (Fig. 1), demonstrating complete replacement of live algae is possible. Although a small delay in development of 1 to 2 days was noted with freeze-dried compared to live algae, these results already demonstrate for the first time that a complete replacement of live algae is possible for raising shrimp from nauplius V to post-larval stage. Upcoming trials

RAS for mollusks Meanwhile, work also started on the development of a RAS for the larval rearing of mollusks, such as cupped and flat oyster (Crassostrea gigas and Ostrea edulis, respectively). A first experimental setup is currently being tested at the Laboratory of Aquaculture & ARC. This system must allow the water to be reused with minimal renewal. It creates a more stable culture environment that, with control of the microbial community, benefits the growth and health of the animals. The RAS technique is already well-established in the aquaculture of fish and shrimp, but is very innovative for mollusks. Focal points are water quality and mineral balance, microbial control and the use of processed algae and automated feeding.

Acknowledgments The BlueMarine³.Com project is funded by the Flemish government through Flanders Innovation and Entrepreneurship (VLAIO) and is facilitated by the Blue Cluster program. It is a collaboration between different groups of Ghent University: the IOF consortium BLUEGent (Dr. ir. Margriet Drouillon), the Laboratory of Aquaculture & Artemia Reference Center (Prof. Dr. ir.

Peter Bossier), the Laboratory for Phycology (Prof. Dr. Olivier De Clerck) and the Laboratory for Environmental Toxicology (Prof. Dr. Colin Janssen), and the following companies: Aquacultuur Oostende, Colruyt Group, DEME, IMAQUA, Proviron and SIOEN. References Oostlander PC, van Houcke J, Wijffels RH, Barbosa MJ. (2020) Microalgae production cost in aquaculture hatcheries. Aquaculture 525.

More information: Mathieu Wille Senior Scientist Laboratory of Aquaculture and Artemia Reference Center, Ghent University, Belgium E: mathieu.wille@ugent.be

Hatchery Feed & Management Vol 8 Issue 4 2020

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BUYERâ&#x20AC;&#x2122;S GUIDE & DIRECTORY 2020 MANUFACTURED FEEDS LIVE FEEDS, ENRICHEMENTS & SUPPLEMENTS HEALTH & DISEASE MANAGEMENT LIVE SUPPLIES HATCHERY SYSTEMS & EQUIPMENT SERVICES & CONSULTING

Hatchery Feed & Management Vol 8 Issue 4 2020

HATCHERY FEED & MANAGEMENT

CATEGORIES & SUPPLIERS

BUYERâ&#x20AC;&#x2122;S GUIDE & DIRECTORY 2020

24

MANUFACTURED FEEDS FISH – Larval - Algafeed - AlgaSpring B.V. - Aller Aqua Group - Allmicroalgae - Natural Products, S.A. - Alltech Coppens - Aquafauna Bio-Marine, Inc. - AquaTech

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- Ocean Aquaculture o Breeder Line OF - - - -

OKM Group Piscine Energetics, Inc. Prairie AquaTech, LLC Primo Aquaculture

- PTAqua o Otohime o Atlantic Gold -

Raanan Fish Feed West Africa

- Reed Mariculture, Inc. o Otohime Fish Diet - -

RICH S.A. Samaki Express E.A. Ltd.

- Skretting AS o GEMMA Micro o GEMMA Wean o CLEAN Start o Perla - Snrassystems - SPAROS Lda. - S.P. Aquatic Science Ltd. (STAVRAS) - Sustainable Nutrition, Inc. - Tromsø Fiskeindustri AS - Varicon Aqua - Zeigler Bros., Inc. FISH – Fry

FISH – Fry - AlgaSpring B.V. - Aller Aqua Group - Allmicroalgae - Natural Products, S.A. - Alltech Coppens - Aquasoja

- BioMar o INICIO Plus M o LARVIVA ProWean - BIOREA - BLUEGent - Cargill - HIGASHIMARU Co., Ltd. - Industrial Plankton, Inc. - INVE Aquaculture - Le Gouessant Aquaculture - Leiber GmbH - MAT RAS - Megasupply - Nutrakol

- Ocean Aquaculture o Breeder Line OF - - - -

OKM Group Phibro Aqua Piscine Energetics, Inc. Primo Aquaculture

Hatchery Feed & Management Vol 8 Issue 4 2020

- PTAqua o Atlantic Gold o Atlantic Gold Sea o VAF Cleanerfish Blocks -

Raanan Fish Feed West Africa

- Rangen o Catfish Feeds o Hybrid Striped Bass Feed o Koi Feeds o Redfish Feeds o Sturgeon Feed o Tilapia Feeds o Trout Feed - -

RICH S.A. Samaki Express E.A. Ltd.

- Skretting AS o GEMMA Silk o CLEAN Assist o PERLA MP - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - The Center for Aquaculture Technologies - Tromsø Fiskeindustri AS - Varicon Aqua - Zeigler Bros., Inc.

FISH – Broodstock - AlgaSpring B.V. - Aller Aqua Group - Allmicroalgae - Natural Products, S.A. - Alltech Coppens - Aquasoja

- BioMar o LARVIVA Maintenance o LARVIVA Maturation - BIOREA - BLUEGent

BUYER’S GUIDE & DIRECTORY 2020

MANUFACTURED FEEDS - Cargill - Industrial Plankton, Inc. - INVE Aquaculture - Le Gouessant Aquaculture - Leiber GmbH - Megasupply - Nutrakol

- Ocean Aquaculture o Krill Pacifica & Superba - - - -

OKM Group Piscine Energetics, Inc. Primo Aquaculture Raanan Fish Feed West Africa

- Rangen o Trout Feed

- Skretting AS o Vitalis PRIMA - Snrassystems - SPAROS Lda. - S.P. Aquatic Science Ltd. (STAVRAS) - The Center for Aquaculture Technologies - Tromsø Fiskeindustri AS - Varicon Aqua - Zeigler Bros., Inc.

- - -

SHRIMP – PL

Industrial Plankton, Inc. INVE Aquaculture ISHE MARICOS

- Algafeed - Aller Aqua Group - Allmicroalgae - Natural Products, S.A. - Aquafauna Bio-Marine, Inc.

- I&V Bio Co., Ltd. o INSTART 1 o INSTART Energy o I&V BIO M o I&V BIO Z

- BioMar o LARVIVA PL - BLUEGent - Cargill - CreveTec - HIGASHIMARU Co., Ltd. - Industrial Plankton, Inc. - INVE Aquaculture - ISHE MARICOS

- Le Gouessant Aquaculture - Leiber GmbH - Megasupply - Molofeed

- Ocean Aquaculture o Fortune Starter+ o Platinum Plus - - - -

OKM Group Piscine Energetics, Inc. Prairie AquaTech, LLC Primo Aquaculture

- PTAqua o Emerald o Super Fresh SV-12 Chlorella -

- I&V Bio Co., Ltd. o INSTART 1 o INSTART Energy o I&V BIO PL 150 o I&V BIO PL 300 o I&V BIO PL 500 o M-Bryo - Le Gouessant Aquaculture - Leiber GmbH - Megasupply - Molofeed

Raanan Fish Feed West Africa

SHRIMP – Larval - Algafeed - Aller Aqua Group - Allmicroalgae - Natural Products, S.A. - Aquafauna Bio-Marine, Inc. - Aquaintech, Inc.

- Reed Mariculture, Inc. o LPB Frozen Shellfish Diet o Shrimp Feeds

- Ocean Aquaculture o Fortune Starter+ o Platinum Plus

- -

- - - -

- BioMar o LARVIVA mysis o LARVIVA zoea

- Skretting AS o PL

- BLUEGent - Cargill - CreveTec - HIGASHIMARU Co., Ltd.

RICH S.A. Shrimp News International

- Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - SyAqua Group Pte. Ltd. - Varicon Aqua - Zeigler Bros., Inc.

OKM Group Piscine Energetics, Inc. Primo Aquaculture Raanan Fish Feed West Africa

- Rangen o Shrimp Feeds

- Reed Mariculture, Inc. o LPB Frozen Shellfish Diet

Hatchery Feed & Management Vol 8 Issue 4 2020

26

MANUFACTURED FEEDS o Shrimp Feeds - -

RICH S.A. Shrimp News International

- -

Primo Aquaculture Raanan Fish Feed West Africa

- - - -

OKM Group Piscine Energetics, Inc. Primo Aquaculture Raanan Fish Feed West Africa

- Rangen o Shrimp Feeds - Skretting AS o PL - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - SyAqua Group Pte. Ltd. - Varicon Aqua - Zeigler Bros., Inc.

- Reed Mariculture, Inc. o LPB Frozen Shellfish Diet o Shrimp Feeds - -

RICH S.A. Shrimp News International

- BioMar o LARVIVA nursery - BLUEGent - Cargill - CreveTec - HIGASHIMARU Co., Ltd. - INVE Aquaculture - ISHE MARICOS

- I&V Bio Co., Ltd. o INSTART Energy o I&V BIO PL 500 - Le Gouessant Aquaculture - Leiber GmbH - Megasupply - Nutrakol

- Ocean Aquaculture o Fortune Starter+ o Platinum Plus - - -

OKM Group Phibro Aqua Piscine Energetics, Inc.

- Reed Mariculture, Inc. o LPB Frozen Shellfish Diet o Shrimp Feeds -

SHRIMP – Juvenile - Aller Aqua Group - Allmicroalgae - Natural Products, S.A. - Aquasoja

- Rangen o Shrimp Feeds

- Skretting AS o PL - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - SyAqua Group Pte. Ltd. - The Center for Aquaculture Technologies - Varicon Aqua - Zeigler Bros., Inc.

SHRIMP – Broodstock - Algafeed - Aller Aqua Group - Allmicroalgae - Natural Products, S.A. - Aquafauna Bio-Marine, Inc. - Aquasoja - BLUEGent - Cargill - CreveTec - Industrial Plankton, Inc. - INVE Aquaculture - ISHE MARICOS - Le Gouessant Aquaculture - Leiber GmbH - MAT RAS (MAT Filtration Technologies) - Megasupply - Nutrakol

- Ocean Aquaculture o Fortune Broodstock+ o Krill Pacifica & Superba

Hatchery Feed & Management Vol 8 Issue 4 2020

Shrimp News International

- Skretting AS o Vitalis 2.5 - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - SyAqua Group Pte. Ltd. - The Center for Aquaculture Technologies - Varicon Aqua - Zeigler Bros., Inc.

MOLLUSCK - Algafeed - Aquasoja - BLUEGent - Industrial Plankton, Inc. - Le Gouessant Aquaculture - Leiber GmbH - OKM Group

- Reed Mariculture, Inc. o LPB Frozen Shellfish Diet o Shellfish Bivalve Feeds o Shellfish Diet 1800 - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - Varicon Aqua

CRAYFISH -

Australian Crayfish Hatchery

BUYER’S GUIDE & DIRECTORY 2020

LIVE FEEDS, ENRICHEMENTS & SUPPLEMENTS Algae products - Algafeed - AlgaSpring B.V. - Algoliner - Aliga Aps - Aquafauna Bio-Marine, Inc. - BIOREA - BLUEGent - G.A.B. srl - inalve - Industrial Plankton, Inc. - Jongerius ecoduna GmbH - LGem | Synalgae - Megasupply - OKM Group - Orkney Shellfish Hatchery

- PTAqua o Emerald o Super Fresh SV-12 Chlorella -

Pure Biomass

- Reed Mariculture, Inc. o Isochrysis 1800 o Nanno 3600 o Pavlova 1800 o Tetraselmis 3600 o TP 1800 o TW 1800 - RICH S.A. - Shrimp News International - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - SyAqua Group Pte. Ltd. - Varicon Aqua

Artemia - - -

AlgaSpring B.V. Aquafauna Bio-Marine, Inc. Aquaintech, Inc.

- Aquatic Equipment and Design, Inc. - BLUEGent - INVE Aquaculture

- I&V Bio Co., Ltd. o INSTART 1 o INSTART Energy o M-Bryo o M-Bryo

- Lallemand Animal Nutrition - Leiber GmbH - LGem | Synalgae - Megasupply - Nutrakol

-

Ocean Aquaculture

- -

OKM Group Primo Aquaculture

- Ocean Aquaculture o Micro Artemia Cysts - OKM Group - Phibro Aqua - Primo Aquaculture - RICH S.A. - Samaki Express E.A. Ltd. - Shrimp News International - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - Sustainable Nutrition, Inc. - SyAqua Group Pte. Ltd. - Tromsø Fiskeindustri AS - Varicon Aqua - Wilfort Aquaculture

Artemia enrichments - -

AlgaSpring B.V. Aquafauna Bio-Marine, Inc.

- BioMar o LARVIVA Multigain - BLUEGent - INVE Aquaculture

-

I&V Bio Co., Ltd.

- PTAqua o Algamac 3050 o Red Algamac - -

RICH S.A. Shrimp News International

- Skretting AS o ORI-N3 - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - Sustainable Nutrition, Inc. - Varicon Aqua

Copepods - AlgaSpring B.V. - AquaTech - ISHE MARICOS - Megasupply

- Ocean Aquaculture o Copepods (fish) o Copepods (shrimp) -

OKM Group

Hatchery Feed & Management Vol 8 Issue 4 2020

28

LIVE FEEDS, ENRICHMENTS & SUPPLEMENTS - PTAqua o Super Fresh SV-12 Chlorella

- Reed Mariculture, Inc. o Instant Zooplankton - Shrimp News, Inc. - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - Varicon Aqua

Live feed production systems - Algoliner - AquaTech - Aquatic Equipment and Design, Inc. - BLUEGent - Industrial Plankton, Inc. - ISHE MARICOS - LGem | Synalgae - OKM Group - Pure Biomass - RICH S.A. - Shrimp News International - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - Sustainable Nutrition, Inc. - Varicon Aqua

Microalgae - Algafeed - AlgaSpring B.V. - Algoliner - Aliga Aps - Allmicroalgae Natural Products, S.A. - Aquatic Equipment and Design, Inc. - BIOREA - BLUEGent - Fresh By Design - G.A.B. srl - inalve

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- PTAqua o Super Fresh SV-12 Chlorella o Emerald -

Pure Biomass

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Polychaetes - ISHE MARICOS - Megasupply

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PTAqua

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Rotifers - AlgaSpring B.V. - Aquatic Equipment and Design, Inc. - BLUEGent - Industrial Plankton, Inc. - ISHE MARICOS - OKM Group - Primo Aquaculture

- Ocean Aquaculture o SPF Polychaetes - - -

OKM Group Piscine Energetics, Inc. Shrimp News International

Hatchery Feed & Management Vol 8 Issue 4 2020

- PTAqua o Super Fresh SV-12 Chlorella o Emerald

Herb extracts, Selenium yeast

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LIVE FEEDS, ENRICHMENTS & SUPPLEMENTS - Snrassystems - Reed Mariculture, Inc. o Instant Zooplankton - -

RICH S.A. Shrimp News International

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- Skretting AS o ORI-Culture o ORI-ONE

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Hatchery Feed & Management Vol 8 Issue 4 2020

S.P. Aquatic Science Ltd. (STAVRAS) Sustainable Nutrition, Inc. Varicon Aqua

Water conditioners - Aquaintech, Inc. - Aquatic Equipment and Design, Inc. - Avecom

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- BiOWiSH Technologies - BLUEGent - INVE Aquaculture

- I&V Bio Co., Ltd. o SHIELD - - - - - -

Keeton Industries, Inc. Lallemand Animal Nutrition Marine Akwa OKM Group Phibro Aqua Primo Aquaculture

-

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SUPER FRESH CHLORELLA ALGAE DHA & EPA ENRICHED FOR OPTIMIZED PRODUCTION OF ROTIFER CULTURES

ATLANTIC GOLD SPECIAL DIET FOR EFFECTIVE CLEANER FISH PRODUCTION RAS SYSTEM COMPATIBLE

VAF FEED BLOCKS

NEW AND UNIQUE FEEDING SOLUTION FOR CLEANER FISH IN SALMON CAGES www.PTAQUA.eu

info@ptaqua.eu

32

HEALTH & DISEASE MANAGEMENT Disease detection - Aquaintech, Inc. - Australian Crayfish Hatchery - BLUEGent - LAB-IND RESOURCE SDN BHD - OKM Group - Phibro Aqua - Shrimp News International - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - The Center for Aquaculture Technologies

Disinfectants

Complete hatchery and nursery feeds for shrimp The LARVIVA feed range includes premium microencapsulated larval feeds for early feeding and co-feeding, extruded granulates for shrimp postlarve, and extruded mini pellets for intensive hatchery and nursery systems.

- AquaTech - Aquatic Equipment and Design, Inc. - BLUEGent - Megasupply - OKM Group - RICH S.A. - Samaki Express E.A. Ltd. - Shrimp News International - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - Syndel - Varicon Aqua - Xylem

Pharmaceuticals - -

OKM Group Phibro Aqua

-

Rangen

- Samaki Express E.A. Ltd. - Searen, LLC - Shrimp News International - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - Syndel

www.larviva.com

Vaccines

HATCHERY

NEXT ISSUE Coming in March

FEED & MANAGEMENT

Special topics: Freshwater fish species: feed and management Live feed production: systems, enrichments, supplements RAS systems: management and technology Nutrition: broodstock, microfeeds, weaning feeds, feed formulation Contact: editor@hatcheryfm.com

Hatchery Feed & Management Vol 8 Issue 4 2020

- - - - -

OKM Group Phibro Aqua Prairie AquaTech, LLC Shrimp News International S.P. Aquatic Science Ltd. (STAVRAS)

Vaccination equipment - - - -

OKM Group Phibro Aqua Shrimp News International S.P. Aquatic Science Ltd. (STAVRAS)

BUYER’S GUIDE & DIRECTORY 2020

LIVE SUPPLIES Eggs - AquaTech - Benchmark Genetics - Dabie Hatchery - OKM Group - Shrimp News International - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - Sustainable Nutrition, Inc. - Troutlodge - Troutex ApS

Larvae - Aquaculture ID - AquaTech - Benchmark Genetics - OKM Group - Shrimp News International - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS)

Fry - Aquaculture ID - ISHE MARICOS - OKM Group - Samaki Express E.A. Ltd. - Shrimp News International - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - Troutlodge

PLs - American Penaeid, Inc. - CreveTec - OKM Group - Shrimp News International - Snrassystems - SyAqua Group Pte. Ltd.

Broodstock – Fish - Aquaculture ID - AquaTech - Benchmark Genetics - ISHE MARICOS - OKM Group - Orkney Shellfish Hatchery - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS)

Concentrating on a good start LARVIVA ProStart is a larval feed for co-feeding and early weaning of fish.

LARVIVA ProStart contains Bactocell®, a unique probiotic approved by the European Commission for its potential in reducing the occurrence of vertebral deformities in fish larvae and fry.

www.larviva.com

Spawning management

Broodstock – Shrimp - American Penaeid, Inc. - Benchmark Genetics Shrimp - ISHE MARICOS - Oceanic Institute of Hawaii Pacific University - OKM Group - Shrimp News International - Snrassystems - SyAqua Group Pte. Ltd.

- BLUEGent - OKM Group - Samaki Express E.A. Ltd. - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - Syndel - Xelect Ltd

Craylings -

Australian Crayfish Hatchery

Hatchery Feed & Management Vol 8 Issue 4 2020

34

HATCHERY SYSTEMS & EQUIPMENT Complete hatchery systems design & construction - Aquacare Environment, Inc. - Aquaculture ID - Aquafauna Bio-Marine, Inc. - AquaTech - Aquatic Equipment and Design, Inc. - Australian Crayfish Hatchery - Clewer Aquaculture Oy - CreveTec - Flexdel Aquaculture Coatings - Fresh By Design - Global Aquaculture Supply - HYDROTECH - Industrial Plankton, Inc. - Innovasea - ISHE MARICOS - JLH Consulting, Inc. - Landing Aquaculture BV - Marine Genetics LLC. - MAT RAS - Nutrakol - Ocean On Land Technology - OKM Group - Pure Biomass - Raanan Fish Feed West Africa - Shrimp News International - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - VAKI Aquaculture Systems - Varicon Aqua

RAS hatchery systems design & construction - Aquacare Environment, Inc. - Aquafauna Bio-Marine, Inc. - AquaMaof Aquaculture Technologies Ltd. - AquaTech - Aquatic Equipment and Design, Inc. - Australian Crayfish Hatchery - BLUEGent - Clewer Aquaculture Oy - CM Aqua Technologies ApS - CreveTec - Delta Hydronics, LLC - Fresh By Design - Global Aquaculture Supply - HYDROTECH - Industrial Plankton, Inc. - Innovasea

- JLH Consulting, Inc. - Landing Aquaculture BV - Lifegard Aquatics - MAT RAS - Ocean On Land Technology - OKM Group - Raanan Fish Feed West Africa - Shrimp News International - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - VAKI Aquaculture Systems - Varicon Aqua - Xylem

Pumps - Advent Environmental Systems/a3® aeration - Aquacare Environment, Inc. - Aquaculture ID - Aquafauna Bio-Marine, Inc. - AquaTech - Aquatic Equipment and Design, Inc. - Australian Crayfish Hatchery - CM Aqua Technologies ApS - FFAZ GmbH - Fresh By Design - Global Aquaculture Supply - Innovasea - ISHE MARICOS - Lifegard Aquatics - LINN Gerätebau GmbH - MAT RAS - Megasupply - OKM Group - Raanan Fish Feed West Africa - RK2 Systems, Inc. - Rotron Store - Samaki Express E.A. Ltd. - Searen, LLC - Shrimp News International - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - VAKI Aquaculture Systems - Varicon Aqua - Xylem

Aerators and oxygenators - Adsorptech - Advent Environmental Systems/a3® aeration - AirSep Corporation - Aquacare Environment, Inc.

Hatchery Feed & Management Vol 8 Issue 4 2020

- Aquafauna Bio-Marine, Inc. - AquaTech - Aquatic Equipment and Design, Inc. - Australian Crayfish Hatchery - CM Aqua Technologies ApS - Dryden Aqua - Enviro Ceramic Ltd. - Fresh By Design - Fresh-flo Corporation - Global Aquaculture Supply - Innovasea - ISHE MARICOS - LINN Gerätebau GmbH - MAT RAS - Megasupply - OKM Group - Oxygen Solutions - Raanan Fish Feed West Africa - Rotron Store - Samaki Express E.A. Ltd. - Searen, LLC - Senect GmbH & Co. KG - Shrimp News International - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - VAKI Aquaculture Systems - Varicon Aqua - Xylem

Heating and cooling - Aquafauna Bio-Marine, Inc. - Aqua Logic, Inc. - AquaTech - Aquatic Equipment and Design, Inc. - Australian Crayfish Hatchery - Delta Hydronics, LLC - Fresh By Design - Global Aquaculture Supply - Innovasea - ISHE MARICOS - MAT RAS - Megasupply - OKM Group - Raanan Fish Feed West Africa - Samaki Express E.A. Ltd. - Shrimp News International - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - Varicon Aqua - Xylem

FIRST IN ALGAE

™

From Start-up to World Leader in Algae Concentrates for Hatcheries Why Rely on Reed Mariculture, Largest Producer of Microalgae Concentrates • We continue to deliver algal products around the world despite supply chain limitations in the industry.

Tim Reed, President, CEO and Founder of Reed Mariculture, Inc

Benefits • Phototrophically grown, highly concentrated marine microalgae.

• The whole-cell algae are non-viable but intact, retaining all nutritional value.

• For 25 years, Reed Mariculture has been advancing the growth of aquaculture globally with the highest quality, commercially available microalgae concentrates.

• Proven effective for all larval growth stages of shrimp, finfish & bivalves.

• Reed Mariculture’s focus is hatcheries. Our algal feeds are used in over 500 hatcheries, research organizations and public aquariums in 90 countries.

• Complete feed replacement or nutritional supplement.

• Consistently available, easy to use.

• Produced by Reed Mariculture in a land-based, biosecure RAS facility.

Tim, Ed and Lyn Reed

Learn more about the benefits of Instant Algae®

ReedMariculture.com

© 2020-21 Reed Mariculture, Inc. All rights reserved. Instant Algae, Algae When You Need It, First In Algae and Ensuring Hatchery Success are trademarks or registered trademarks of Reed Mariculture Inc.

36

HATCHERY SYSTEMS & EQUIPMENT Water treatment - Advent Environmental Systems/a3® aeration - Aquacare Environment, Inc. - Aquafauna Bio-Marine, Inc. - AquaTech - Aquatic Equipment and Design, Inc. - Australian Crayfish Hatchery - BLUEGent - Clewer Aquaculture Oy - CM Aqua Technologies ApS - CreveTec - Dryden Aqua - Fresh By Design - Global Aquaculture Supply - HYDROTECH - Innovasea - ISHE MARICOS - Keeton Industries, Inc. - Landing Aquaculture BV - Lifegard Aquatics - LINN Gerätebau GmbH - MAT RAS - Megasupply - OKM Group - OxyGuard International A/S - Raanan Fish Feed West Africa - RK2 Systems, Inc. - Rotron Store - Samaki Express E.A. Ltd. - Searen, LLC - Senect GmbH & Co. KG - Shrimp News International - Snrassystems - Varicon Aqua - Xylem

Tanks - Aquacare Environment, Inc. - Aqua Logic, Inc. - AquaTech - Aquatic Equipment and Design, Inc. - Australian Crayfish Hatchery - CM Aqua Technologies ApS - Fresh By Design - Global Aquaculture Supply - Innovasea

- ISHE MARICOS - Landing Aquaculture BV - MAT RAS - Megasupply - OKM Group - Raanan Fish Feed West Africa - Samaki Express E.A. Ltd. - Shrimp News International - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - Varicon Aqua

Lighting - AquaTech - Aquatic Equipment and Design, Inc. - Australian Crayfish Hatchery - Bioled - BLUEGent - FFAZ GmbH - Fresh By Design - Global Aquaculture Supply - Innovasea - ISHE MARICOS - Lifegard Aquatics - OKM Group - Pure Biomass - Raanan Fish Feed West Africa - Shrimp News International - Signify - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - Varicon Aqua

Monitoring - Aquacare Environment, Inc. - Aquafauna Bio-Marine, Inc. - AquaTech - Aquatic Equipment and Design, Inc. - Algaeba Company Limited - Australian Crayfish Hatchery - Biomark - BLUEGent - Fresh By Design - Global Aquaculture Supply - Innovasea - LINN Gerätebau GmbH - MAT RAS

Hatchery Feed & Management Vol 8 Issue 4 2020

- OKM Group - OxyGuard International A/S - Raanan Fish Feed West Africa - Senect GmbH & Co. KG - Shrimp News International - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - VAKI Aquaculture Systems - Varicon Aqua - Wittaya Aqua - XpertSea - Xylem

Software - Aquacare Environment, Inc. - AquaTech - Australian Crayfish Hatchery - BLUEGent - Fresh By Design - Global Aquaculture Supply - Innovasea - Jensorter - LINN Gerätebau GmbH - OKM Group - OxyGuard International A/S - Senect GmbH & Co. KG - Shrimp News International - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - VAKI Aquaculture Systems - Varicon Aqua - Wittaya Aqua - XpertSea - Xylem

Feeders and feeding systems - Aquacare Environment, Inc. - Algafeed - AquaTech - Aquatic Equipment and Design, Inc. - eFishery - FFAZ GmbH - FishFarmFeeder - Fresh By Design - Fresh-flo Corporation

38

HATCHERY SYSTEMS & EQUIPMENT HIGH TEMPERATURE

Hydro-Mix HT

When Quality Matters, Choose Quality Moisture Sensors

- Global Aquaculture Supply - Industrial Plankton, Inc. - Innovasea - LINN Gerätebau GmbH - Megasupply - Nutrakol - Ocean On Land Technology - OKM Group - Piscine Energetics, Inc. - Pure Biomass - Samaki Express E.A. Ltd. - Senect GmbH & Co. KG - Shrimp News International - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - Zeigler Bros., Inc.

Grading and counting systems Hydro-Mix XT • Simple and cost-effective way to control your dryers • Improve the quality of your final product • Digital technology with precise linear output • Install in silos, conveyors or the Hydronix ducting system • Wide moisture measurement range • Not affected by dust or colour • Temperature stable

- Algaeba Company Limited - Aquacare Environment, Inc. - AquaTech - Aquatic Equipment and Design, Inc. - Australian Crayfish Hatchery - Calitri technology - Fresh By Design - Fresh-flo Corporation - Global Aquaculture Supply - Innovasea - OKM Group - Raanan Fish Feed West Africa - Samaki Express E.A. Ltd. - Shrimp News International - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - VAKI Aquaculture Systems - XpertSea

enquiries@hydronix.com www.hydronix.com

0344-Hydronix-Advert - Grain quality moisture sensors - Hatchery 24/11/2020 & Feed Dec10:59 228.6x58.4 Folder.indd 1

Hatchery Feed & Management Vol 8 Issue 4 2020

Egg sorters and incubators - Aqua Logic, Inc. - AquaTech - Australian Crayfish Hatchery - Fresh By Design - Global Aquaculture Supply - Jensorter - OKM Group - Redd Zone - Shrimp News International - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS)

Packaging - AquaTech - Global Aquaculture Supply - Fresh By Design - OKM Group - Samaki Express E.A. Ltd. - Shrimp News International - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS)

Screens and mesh - AquaTech - Aquatic Equipment and Design, Inc. - CM Aqua Technologies ApS - Fresh By Design - Global Aquaculture Supply - HYDROTECH - Innovasea - Megasupply - OKM Group - Samaki Express E.A. Ltd. - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - Varicon Aqua

40

SERVICES & CONSULTING Genomics and breeding - Aquaintech, Inc. - American Penaeid, Inc. - Australian Crayfish Hatchery - Benchmark Genetics - BLUEGent - GenetiRate, Inc. - LAB-IND RESOURCE SDN BHD - Marine Genetics LLC. - Oceanic Institute of Hawaii Pacific University - OKM Group - Raanan Fish Feed West Africa - Snrassystems - SyAqua Group Pte. Ltd. - The Center for Aquaculture Technologies - Troutex ApS - Troutlodge - Wittaya Aqua - Xelect Ltd.

Hatchery management - Aquaculture ID - Aquaintech, Inc. - AquaTech - Australian Crayfish Hatchery - BLUEGent - CreveTec - Innovasea - INVE Aquaculture - ISHE MARICOS - Marine Genetics LLC. - MAT RAS - Megasupply - Molofeed - Orkney Shellfish Hatchery - OKM Group - OxyGuard International A/S - Raanan Fish Feed West Africa - Samaki Express E.A. Ltd. - Snrassystems - Syndel - Troutex ApS - Wittaya Aqua

Zoo - and phytoplankton - Algafeed - AlgaSpring B.V. - Aquaintech, Inc. - AquaTech - ISHE MARICOS - OKM Group - Piscine Energetics, Inc.

-

PTAqua

- Raanan Fish Feed West Africa - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS)

Feed development - Algafeed - AlgaSpring B.V. - Aller Aqua Group - Allmicroalgae Natural Products, S.A. - Aquaintech, Inc. - Aquasoja - Australian Crayfish Hatchery - Avecom - BLUEGent - Cargill - CreveTec - BIOREA - BiOWiSH Technologies - Lallemand Animal Nutrition - Le Gouessant Aquaculture - MAT RAS - Megasupply - Molofeed - Nutrakol - OKM Group - Phibro Aqua - Pontus Research Ltd. - Prairie AquaTech, LLC

-

PTAqua

- -

Pure Biomass Raanan Fish Feed West Africa

Hatchery Feed & Management Vol 8 Issue 4 2020

-

Rangen

- Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - -

- -

SPAROS Lda. The Center for Aquaculture Technologies Tromsø Fiskeindustri AS Wittaya Aqua

Nutrition consulting - Algafeed - AlgaSpring B.V. - Aquaintech, Inc. - Aquasoja - Australian Crayfish Hatchery - BLUEGent - Cargill - CreveTec - G.A.B. srl - INVE Aquaculture - Le Gouessant Aquaculture - Megasupply - Molofeed - Nutrakol - OKM Group - Phibro Aqua - Piscine Energetics, Inc. - Pontus Research Ltd. - Prairie AquaTech, LLC - Pure Biomass - Raanan Fish Feed West Africa

-

Rangen

- RICH S.A. - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - SPAROS Lda. - The Center for Aquaculture Technologies - Wittaya Aqua

Water quality - AlgaSpring B.V. - Aquaintech, Inc. - AquaTech

42

SERVICES & CONSULTING - Australian Crayfish Hatchery - Avecom - BiOWiSH Technologies - BLUEGent - CM Aqua Technologies ApS - CreveTec - HYDROTECH - Innovasea - ISHE MARICOS - Lallemand Animal Nutrition - MAT RAS - OKM Group - OxyGuard International A/S - Phibro Aqua - Pontus Research Ltd.

-

PTAqua

- Pure Biomass - Raanan Fish Feed West Africa - Senect GmbH & Co. KG - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - Wittaya Aqua

Pathology - AlgaSpring B.V. - Aquaintech, Inc. - Australian Crayfish Hatchery - BLUEGent - LAB-IND RESOURCE SDN BHD - OKM Group - Phibro Aqua - Pontus Research Ltd. - Raanan Fish Feed West Africa - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - Wittaya Aqua

RAS - Aquaculture ID - Aquaintech, Inc. - AquaMaof Aquaculture Technologies Ltd. - Aquatic Equipment and Design, Inc. - Australian Crayfish Hatchery - BLUEGent - Clewer Aquaculture Oy - CM Aqua Technologies ApS

- CreveTec - Delta Hydronics, LLC - Fresh By Design - Innovasea - ISHE MARICOS - JLH Consulting, Inc. - Phibro Aqua - Landing Aquaculture BV - MAT RAS - Megasupply - Ocean On Land Technology - OKM Group - OxyGuard International A/S - Pontus Research Ltd. - Raanan Fish Feed West Africa - Searen, LLC - Senect GmbH & Co. KG - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - Wittaya Aqua

Systems and technology - AlgaSpring B.V. - Aquaculture ID - Aquaintech, Inc. - AquaTech - Australian Crayfish Hatchery - Biomark - Clewer Aquaculture Oy - CM Aqua Technologies ApS - CreveTec - Deep Trekker - Delta Hydronics, LLC - Dryden Aqua - FFAZ GmbH - HYDROTECH - Industrial Plankton, Inc. - Innovasea - ISHE MARICOS - JLH Consulting, Inc. - Landing Aquaculture BV - LGem | Synalgae - MAT RAS - Megasupply - Nutrakol - Ocean On Land Technology - OKM Group - Oxygen Solutions - OxyGuard International A/S - Phibro Aqua - Pontus Research Ltd.

Hatchery Feed & Management Vol 8 Issue 4 2020

- Pure Biomass - Raanan Fish Feed West Africa - Redd Zone - Samaki Express E.A. Ltd. - Senect GmbH & Co. KG - Snrassystems - S.P. Aquatic Science Ltd. (STAVRAS) - VAKI Aquaculture Systems - Varicon Aqua - Wittaya Aqua Environmental

Environmental Services - Aquaintech, Inc. - Avecom - BiOWiSH Technologies - FFAZ GmbH - ISHE MARICOS - MAT RAS - OKM Group - Raanan Fish Feed West Africa - Snrassystems - Varicon Aqua - Wittaya Aqua

Research - Algafeed - Allmicroalgae - Natural Products, S.A. - BIOREA - BLUEGent - Oceanic Institute of Hawaii Pacific University - Orkney Shellfish Hatchery - Pontus Research Ltd. - Prairie AquaTech, LLC - SPAROS Lda. - S.P. Aquatic Science Ltd. (STAVRAS) - The Center for Aquaculture Technologies - Wittaya Aqua

Consultancy - Efstathios - ISHE MARICOS - Phibro Aqua - Varicon Aqua

HATCHERY FEED & MANAGEMENT

A-Z OF COMPANIES

BUYERâ&#x20AC;&#x2122;S GUIDE & DIRECTORY 2020

44

A-Z OF COMPANIES Adsorptech Sales Office, 22 Stonebridge Rd Hampton, NJ 08827, USA www.adsorptech.com E: james.flaherty@adsorptech.com

Aller Aqua Group Allervej 130 6070 Christiansfeld, Denmark www.aller-aqua.com E: info@aller-aqua.com

Aquaintech, Inc. 16825 48th Ave W Suite 322 Lynnwood, WA 98037, USA www.aqua-in-tech.com E: sgnewm@aqua-in-tech.com

Advent Environmental Systems / a3® aeration 37472 Hills Tech Drive Farmington Hills, MI 48331, USA www.a3aeration.com E: bob@a3aeration.com

Allmicroalgae - Natural Products, S.A. Rua 25 de Abril, s/n Pataias – Gare 2445 - 413 Pataias, Portugal www.allmicroalgae.com E: rita.mateus@allmicroalgae.com

AquaMaof Aquaculture Technologies Ltd. 12 Amal St. Rosh Ha’ayin, Israel www.aquamaof.com E: info@aquamaof.com

AirSep Corporation 260 Creekside Drive Buffalo, NY 14228, USA www.airsep.com E: info@airsep.com

Alltech Coppens Dwarsdijk 4 5705 DM Helmond, The Netherlands www.alltechcoppens.com E: coppens@alltech.com

Aquasoja Estrada 109, Lugar da Pardala 3880-728 S. João Ovar, Portugal www.aquasoja.pt E: aquasoja@sojadeportugal.pt

Algaeba Company Limited 64/32 Moo 4, Therdphrakieat Rd Wat Chalor, Bangkruai, Nonthaburi, Thailand 11130 www.algaeba.com E: kunn.k@algaeba.com

American Penaeid, Inc. 9703 Stringfellow Road St James City, FL 33956, USA penaeid.com E: info@penaeid.com

AquaTech Unterbrunnweg 3 6370 Kitzbühel, Austria www.aqua-tech.eu E: aquatech@a1.net

Algafeed 3125 Jupiter Park Circle - Unit 2 Jupiter, FL 33458, USA algafeed.com E: mwulff@algafeed.com

Aqua Logic, Inc. 9558 Camino Ruiz San Diego, CA 92126 ,USA www.aqualogicinc.com E: info@aqualogicinc.com

Aquatic Equipment and Design, Inc. 18981 HWY 441 #329 Mount Dora, FL 32757, USA www.aquaticed.com E: Amy@aquaticed.com

AlgaSpring B.V. E. Heimansweg 16, 1331AP, Almere, The Netherlands www.algaspring.nl E: sales@algaspring.nl

Aquacare Environment, Inc. 7087 Coho Way Bellingham, WA 98225, USA aquacare.com info@aquacare.com

Australian Crayfish Hatchery 21 Everett St Mount St John, Qld 4818, Australia redclawhatchery.com.au E: marketing@redclawhatchery.com.au

Algoliner Lindenweg 14, 64409 Messel, Germany www.algoliner.de E: Hans.Vaeth@Algoliner.de

Aquaculture ID Eindhovensebaan 9 6031 NB Nederweert, The Netherlands www.aquacultureid.com info@aquacultureid.com

Avecom Industrieweg 122P 9032 Wondelgem, Belgium www.avecom.be E: sales@avecom.be

Aliga Aps Vandvaerksvej 12 9800 Hjoerring, Denmark aliga.dk E: de@aliga.dk

Aquafauna Bio-Marine, Inc. PO Box 5 Hawthorne, CA 90250, USA www.aquafauna.com E: budinsalata@aquafauna.com

Benchmark Genetics Sandviksboder 3ª 5035 Bergen, Norway www.bmkgenetics.com E: post@bmkgenetics.com

Hatchery Hatchery Feed Feed & Management & Management Vol Vol 8 Issue 8 Issue 4 2020 4 2020

BUYER’S GUIDE & DIRECTORY 2020

Benchmark Genetics Shrimp 15369 County Road 512 Fellsmere, FL 32948, USA shrimp.bmkgenetics.com E: shrimp@bmkgenetics.com Bioled Loteo 17, parcelación San Juan Puerto Varas, Chile www.bioled.cl E: contacto@bioled.cl BioMar 60, Rue Pierre-Georges Debouchaud, Zone Industrielle 16440 Nersac, France www.larviva.com E: mla@biomar.com Biomark 705 S. 8th Street Boise, Idaho 83702, USA www.biomark.com E: customerservice@biomark.com BIOREA 10 Rue Ampère 22400, Lamballe, France www.biorea.fr E: contact@biorea.fr BiOWiSH Technologies 2717 Erie Ave. Cincinnati, OH 45208, USA biowishaquaculture.com E: contact@biowishtech.com

Cargill 15407 McGinty Road West Wayzata, MN 55391, USA www.cargill.com/animal-nutrition Clewer Aquaculture Oy Linnankatu 34 20100 Turku, Finland www.cleweraquaculture.com E: riitta.myyra@clewer.com CM Aqua Technologies ApS Farum Gydevej 64 Farum 3520, Denmark www.cmaqua.dk E: info@cmaqua.dk

eFishery Jl. Bukit Pakar Timur IV Kav. B1, Ciburial Kecamatan Cimenyan, Bandung, Jawa Barat, 40198, Indonesia efishery.com E: junia.anindya@efishery.com Enviro Ceramic Ltd Carrigbawn House Drinagh, co.Cork, Ireland enviroceramic.com E: info@enviroceramic.com FFAZ GmbH Klosterstrasse 34 88512 Mengen, Germany ffaz-fishfeeder.com E: info@ffaz-fishfeeder.com

CreveTec Heirbaan 56ª 1740 Ternat, Belgium www.crevetec.be E: eric@crevetec.be

FishFarmFeeder Carretera de Madrid 152 36318 Vigo (Pontevedra) Spain www.fishfarmfeeder.com E: fishfarmfeeder@fishfarmfeeder.com

Dabie Hatchery Spacerowa 12 Bytow, Poland www.ikra-dabie.pl E: sales@ikra-dabie.pl Deep Trekker 830 Trillium Drive Kitchener, Ontario, Canada www.deeptrekker.com E: rdoornekamp@deeptrekker.com

Flexdel Aquaculture Coatings 14805 49th Street Clearwater Florida 33762, USA www.flexdel.com E: khester@seahawkpaints.com Freelance consultant Dubai E: stathisstoi@yahoo.gr

BLUEGent Coupure Links 653 9000 Gent, Belgium www.bluegent.ugent.be E: bluegent@ugent.be

Delta Hydronics, LLC 9100 Bolton Ave Hudson, FL 34667, USA www.deltahydro.com E: susanb@deltahydro.com

Fresh By Design 13 Old Dairy Close Moss Vale, NSW 2577, Australia freshbydesign.com.au E: lachlan@freshbydesign.com.au

Calitri technology 14, rue de la botte 5650 Yves-Gomezée, Belgium www.calitri-technology.com E: info@calitri-technology.com

Dryden Aqua Butlerfield Ind. Est Bonnyrigg Edinburgh,EH19 3JQ, UK www.drydenaqua.com E: mike.demaine@drydenaqua.com

Fresh-flo Corporation 3037 Weeden Creek Road Sheboygan, WI 53081, USA www.freshflo.com E: barb@freshflo.com

Hatchery Hatchery Feed Feed & Management & Management Vol Vol 8 Issue 8 Issue 4 2020 4 2020

46

G.A.B. srl Via Nizza 20 37121 Verona, Italy E: commerciale@gruppogab.it GenetiRate, Inc. 4561 N Arroyo Vacio Tucson, AZ 85750, USA E: ben.renquist@genetirate.fish Global Aquaculture Supply 1001 Industrial Ave Alton, IA 51003, USA globalaquaculturesupply.com E: m.fudge@globalaquaculturesupply.com HIGASHIMARU Co., Ltd. 20 Igakura, Ijuin-cho, Hioki-city Kagoshima, 899-2594, Japan www.k-higashimaru.co.jp E: shiryo@k-higashimaru.co.jp HYDROTECH Mejselgatan 6, 235 32 Vellinge, Sweden www.hydrotech.se E: mailbox@hydrotech.se I&V Bio Co.,Ltd. 88/49-50 BangbauthongPathumthani Road Bangtanai Sub-district, Pakkred District, Nonthaburi 11120, Thailand www.iandv-bio.com E: sales@iandv-bio.com inalve CEEI, 61-63 Avenue Simone Veil, inalve, Nice, 06200, France www.inalve.com E: contact@inalve.com

INVE Aquaculture Hoogveld 93 9200 Dendermonde, Belgium www.inveaquaculture.com E: info@inveaquaculture.com

Le Gouessant Aquaculture 1 rue de la Jeannaie 22400 Lamballe, France aqua.legouessant.com E: contact.aqua@legouessant.com

ISHE MARICOS 42/2491 Power House Road Cochin 682018, Kerala, India www.marineconsultantindia.com E: popychenkavalam@yahoo.com

Leiber GmbH Hafenstr. 24, 49565 Bramsche, Germany www.leibergmbh.de E: info@leibergmbh.de

Jensorter 2660 SE 39th Loop Suite D, Hillsboro OR, USA www.jensorter.com E: jensorter@gmail.com

LGem | Synalgae Achterweg 65 3029 AL Rotterdam, The Netherlands lgem.nl E: sander.hazewinkel@lgem.nl

JLH Consulting, Inc. 606 Evergreen Ave., Courtenay, BC V9N 7N5, Canada jlhconsulting.tv E: jlhconsulting@shaw.ca

Lifegard Aquatics 8633 Sorensenn Ave. Santa Fe Springs, CA 90670, USA www.lifegardaquatics.com E: info@lifegardaquatics.com

Jongerius ecoduna GmbH Eco-Plus Park 1. Straße 8 2460 Bruck an der Leitha, Austria www.jongerius-ecoduna.at E: sales@jongerius-ecoduna.at Keeton Industries, Inc. 1520 Aquatic Drive Wellington, Colorado, USA www.keetonaqua.com E: info@keetonaqua.com LAB-IND RESOURCE SDN BHD 57-59 Jalan Adenium 2G/6, Bukit Beruntung 48300 Rawang, Selangor, Malaysia mylabind.wixsite.com E: info@mylabind.com

LINN Gerätebau GmbH D-57368 Lennestadt An der Sauerlandkaserne 1, Germany www.linn.eu E: info@linn.eu Marine Akwa 64 Grande Rue 22750 Saint Jacut de la Mer, France marineakwa.com E: fanny@marineakwa.com Marine Genetics LLC PO Box 1095 Kurtistown, HI 96760, USA E: jim.wyban@gmail.com

Industrial Plankton, Inc. Victoria, BC, Canada industrialplankton.com E: info@industrialplankton.com

Lallemand Animal Nutrition 6120 W. Douglas Ave. Milwaukee, WI 53218, USA lallemandanimalnutrition.com E: lannamarketing@lallemand.com

MAT RAS (MAT FILTRATION TECHNOLOGIES) 124 Istikbal Cad. Güzelbahçe, Izmir, Turkey mat-ras.com E: info@mat-ras.com

Innovasea 17445 Opportunity Avenue Baton Rouge, LA 70817, USA www.innovasea.com E: landsystems@innovasea.com

Landing Aquaculture BV Hemelrijk 2a 5281 PS Boxtel, The Netherlands www.landingaquaculture.com E: info@landingaquaculture.com

Megasupply 3559 NW 82nd Avenue Miami Florida, 33122, USA www.megasupply.com E: orders@megasupply.net

Hatchery Hatchery Feed Feed & Management & Management Vol Vol 8 Issue 8 Issue 4 2020 4 2020

BUYER’S GUIDE & DIRECTORY 2020 Molofeed AS Eidsvagveien 150 N-5105 Eidsvag, Norway www.molofeed.no E: thorbjorn.toppe@molofeed.no

Oxygen Solutions 5304 - 36 Street NW Edmonton Alberta, T6B 3P3, Canada osioxygen.com E: chris.miller@benchmarkinc.ca

QB Labs 13741 Rider Trail N Earth City MO, 63045, USA www.qb-labs.com E: sales@qb-labs.com

Nutrakol 27 Korella street Mullaloo, 6027 WA, Australia www.nutrakol.com E: info@nutrakol.com

OxyGuard International A/S Farum Gydevej 64 3520 Farum, Denmark www.oxyguard.dk E: oxyguard@oxyguard.dk

Raanan Fish Feed West Africa Prampram Factory Ghana www.raananfishfeed-wa.com E: info@raananfishfeed-wa.com

Ocean Aquaculture Rijkmakerlaan 15 2910 Essen, Belgium Marine Nutrition Co. Ltd. 354 Moo 1 Rangrotfaisaigaow Road Tambon Sumrongtai, Amphur Prapradeang 10130 Samutprakarn, Thailand www.ocean-aquaculture.com E: info@ocean-aquaculture.com Ocean On Land Technology Unit 2 Pastures Farm, 600 Station Road Grendon, Northamptonshire, NN7 1JD, UK www.oceanonland.com E: info@oceanonland.com Oceanic Institute of Hawaii Pacific University 41-202 Kalanianaole Highway Waimanalo, Hawaii 96795-1820, USA oceanicinstitute.org E: smoss@hpu.edu OKM GROUP 63 Jublee Road, Enayet Bazar Chittagong, Bangladesh okmgroupbd.com E: rahaman.ghl@gmail.com Orkney Shellfish Hatchery The Lobster Ponds, Lamb Holm Quarry, Burray Orkney, KW17 2SF, UK www.orkneyshellfishhatchery.co.uk E: info@orkneyshellfishhatchery.co.uk

Phibro Aqua Industrial Area Ma’ayan Zvi, Israel www.phibro-aqua.com E: aqua@pahc.com

Rangen 115 13th Ave S Buhl, Idaho 83316, USA www.rangen.com Contact: www.rangen.com

Piscine Energetics Inc. 1015 Fairweather Road Vernon, BC, V1T 8T8, Canada www.mysis.com E: aquaculture@mysis.com Pontus Research Ltd. Unit E Hirwaun Industrial Estate Hirwaun, Aberdare CF44 9UP, UK www.pontusresearch.com E: jmj@pontusresearch.com Prairie AquaTech, LLC 101 N. Main Avenue, Suite 325 Sioux Falls, SD 57104, USA www.prairieaquatech.com E: sales@prairieaquatech.com Primo Aquaculture 4/ 31 Robart Court Narangba, QLD, 4504, Australia www.primo.net.au E: primo@ridley.com.au PTAqua 67e Heather Rd, Sandyford Ind. Est. Dublin, D18NV90, Ireland www.ptaqua.eu E: ian@ptaqua.eu Pure Biomass 441 Saxony RD Encinitas, CA 92024, Canada www.purebiomass.org E: info@purebiomass.com

Redd Zone 1125 16th St#1 Astoria, Or 97103, USA reddzone.net E: tod@reddzone.net Reed Mariculture, Inc. 900 E Hamilton Ave Suite 100, Campbell, CA 95008, USA www.reedmariculture.com E: sales@reedmariculture.com RICH S.A. 2-4 Afroditis Street, 17561, P. Faliro, Athens, Greece rich.gr E: rich@rich.gr RK2 Systems, Inc. 225 South Bent Avenue San Marcos, CA 92078, USA www.RK2.com E: sales@rk2.com Rotron Store 12345 Pauls Valley Road, B-6 Austin, Texas 78737, USA www.rotronstore.com E: orders@sivatsi.com Samaki Express E.A. Ltd. Nairobi, Kenya www.samakiexpress.com E: am@samakiexpress.co.ke

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Searen, LLC 3846 Marburg Ave. Cincinnati, OH, USA www.searen.com E: contact@searen.com

Sustainable Nutrition, Inc. 110 W Old Andrew Johnson Highway Jefferson City, TN 37760, USA www.snextracts.com E: tim@snextracts.com

Senect GmbH & Co. KG An 44 No. 11 76829 Landau, Germany www.senect.de E: info@senect.de

SyAqua Group Pte. Ltd. 101 Thomson Road, United Square #30-05 307591, Singapore syaqua.com E: ricardo.mello@syaqua.com

Shrimp News International 1448 Rancho Drive Hollister, CA 95023, USA www.shrimpnews.com E: bob@shrimpnews.com Signify (former Philips Lighting) High Tech Campus 7 5656 AE Eindhoven, The Netherlands www.philips.com/aquaculture E: daniela.damoiseaux@signify.com Skretting AS Sjøhagen 3 4016 Stavanger, Norway www.skretting.com E: eamonn.obrien@skretting.com Snrassystems Pune, India www.snrassystems.com E: snrassystems@gmail.com SPAROS Lda. Área Empresarial de Marim, Lote C 8700-221 Olhão, Portugal www.sparos.pt E: sparos@sparos.pt S.P. Aquatic Science Ltd. (STAVRAS) S.P. Aquatic Science Ltd. (STAVRAS) Saint Marina 7 5288 Paralimni, Cyprus www.stavrasaquatic.com E: info@stavrasaquatic.com

SYNDEL 1441 W Smith Road Ferndale, WA USA syndel.com E: sales@syndel.com The Center for Aquaculture Technologies 20 Hope Street Souris, PEI C0A 2B0, Canada aquatechcenter.com E: info@aquatechcenter.com Tromsø Fiskeindustri AS Stakkevollvegen 57 N-9010 Tromsø, Norway www.trofico.no E: trofico@oddberg.no Troutex ApS Aftensang 10G DK6040 Egtved, Denmark www.troutex.dk E: mail@troutex.dk Troutlodge P.O. Box 1290 Sumner, WA 98390, USA www.troutlodge.com E: trout@troutlodge.com VAKI Aquaculture Systems Akralind 4 201 Kopavogur, Iceland www.vaki.is E: vaki@merck.com

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Varicon Aqua Unit 12, Ball Mill Top Business Park, Hallow, Worcester WR2 6PD, UK www.variconaqua.com E: marcolizzul@variconaqua.com Wilfort Aquaculture 3122 Century Drive Oregon 97601, USA www.wilfortinc.com E: sales@wilfortinc.com Wittaya Aqua 1 University Ave, Suite 300 Toronto, ON, M5J 2P1, Canada www.wittaya-aqua.ca E: info@wittaya-aqua.ca Xelect Ltd. Horizon House, Abbey Walk St Andrews, Scotland, KY16 9LB, UK www.xelect-genetics.com E: info@xelect.co.uk XpertSea 1365 Galilée Ave, suite 100 Québec, QC G1P 4G4, Canada www.xpertsea.com E: info@xpertsea.com Xylem Fetveien 23 2007 Kjeller, Norway www.xylem.com E: firmapost@xyleminc.com Zeigler Bros., Inc. 400 Gardners Station Road Gardners, PA 17324, USA www.zeiglerfeed.com E: info@zeiglerfeed.com

Industry Events

Send your meeting details to: editor@hatcheryfm.com

2021 FEBRUARY 9 - 12:

EuroTier, Hanover, Germany

www.eurotier.com

Purchasing & Ingredients Suppliers Conference, Orlando, USA LACQUA, Guayaquil, Ecuador

www.afia.org was.org

Aquaculture Europe 2020, Virtual event

www.aquaeas.org

6th International Symposium on Genomics in Aquaculture World Aquaculture and Fisheries Conference, Tokyo, Japan Aquaculture UK, Scotland, UK IDMA and VICTAM, Istanbul, Turkey

www.gia2020.es worldaquacultureconference.com aquacultureuk.com idmavictam.com

International Symposium of Fish Nutrition and Feeding, Busan, South Korea World Aquaculture 2020, Singapore 19th International congress on Animal Reproduction

www.isfnf2020busan.com was.org www.icar2020.com

ILDEX Vietnam, Ho Chi Minh City, Vietnam

www.ildex-vietnam.com

Aquaculture America, San Antonio, USA The Aquaculture Round Table Series, Ho Chi Minh City, Vietnam

www.was.org www.tarsaquaculture.com

MARCH 9 - 11:

22 - 25:

APRIL 12 - 15:

MAY 5 - 7: 19 - 20: 19 - 21: 27 - 29:

JUNE 6 - 11:

14 - 18: 27 - Jul 1:

JULY 21 - 23:

AUGUST 11 - 14: 18 - 19:

SEPTEMBER 7 - 10: 22 - 24: 26 - 29:

Asian Pacific Aquaculture 2021, Surabaya, Indonesia VIV Asia 2021, Bangkok, Thailand Aquaculture Canada and WAS North America 2021, Newfoundland, Canada

www.marevent.com www.vivasia.nl www.was.org

Hatchery Feed & Management Vol 8 Issue 4 2020

WELCOME

NEW DATES !

Singapore - June 14-18, 2021 Singapore EXPO Convention and Exhibition Centre

Hosted by Singapore Food Agency Organized by World Aquaculture Society

WA

Next generation Aquaculture Innovation and sustainability will feed the world

Someformofhuman

Hashtags: #WA2020 Facebook: Wa2020 Linkdin: WASAPC

For more info on the CONFERENCE: www.was.org - worldaqua@was.org For more info on the TRADESHOW: mario@marevent.com