PATHOGENIC BACTERIA
CATALOGUE
In the scope of Biobanco Azul Português



BACTERIA CATALOGUE

ORIGIN:
Algarve, Portugal
HOST: Sparus aurata
TISSUE: Whole larvae
CULTURE MEDIUM: TSA/TCBS
ANTIBIOTIC SENSITIVITY: OT, ENR, FFC and UB
MORTALITY: 3%/day

BIOCHEMICAL CHARACTERIZATION: TBA
STORAGE DATE: 2023
MOLECULAR CHARACTERIZATION:
The clone was identified by PCR as Vibrio harveyi.
The clone expresses four virulence genes (out of 6 tested) – topA, flaA, recA and fur.


ORIGIN: Algarve, Portugal
HOST: Dicentrarchus labrax
TISSUE: Kidney
CULTURE MEDIUM: TSA/TCBS
ANTIBIOTIC SENSITIVITY:
OT, ENR, UB and FFC

BIOCHEMICAL CHARACTERIZATION:
Colour: Yellow
Form: Bacilococus
Gram: -
Mobility: +
Oxidase: -/+
Fermentation: + Aminoacids (ODC/LDC/ADC): -/+/-
STORAGE DATE: 2023
MOLECULAR CHARACTERIZATION:
The clone was identified by PCR as Vibrio harveyi
The clone expresses six virulence genes (out of 6 tested) - topA, gyrB, toxR, flaA, recA and fur


BACTERIA CATALOGUE
Aeromonas hydrophila #A165
ORIGIN: Algarve, Portugal
HOST: Danio rerio
TISSUE: Liver
ANTIBIOTIC SENSITIVITY: TBA
BIOCHEMICAL CHARACTERIZATION: TBA
CULTURE MEDIUM:
TSA/Basal Medium
STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION: The clone was identified by PCR as Aeromonas hydrophila.

BACTERIA CATALOGUE
Photobacterium damselae subs. piscicida #A157

ORIGIN: Algarve, Portugal
HOST: Dicentrarchus labrax
TISSUE: Liver
ANTIBIOTIC SENSITIVITY:
OT, ENR, UB and FFC

BIOCHEMICAL CHARACTERIZATION: TBA
CULTURE MEDIUM:
TSA/Blood agar
STORAGE DATE: 2022
MOLECULAR CHARACTERIZATION:
The clone was identified by PCR as Photobacterium damselae subs. piscicida.
The clone expresses seven virulence genes (out of 7 tested) - toxR, aip56, gyrB, recA, fur, irp2, and frpA.

BACTERIA CATALOGUE

ORIGIN:
Algarve, Portugal
HOST: Dicentrarchus labrax
TISSUE: Spleen
ANTIBIOTIC SENSITIVITY: OT, ENR and FMM

BIOCHEMICAL CHARACTERIZATION: TBA
CULTURE MEDIUM: TSA/TCBS
STORAGE DATE: 2023
MOLECULAR CHARACTERIZATION:
The clone was identified by PCR as Vibrio anguillarum.
The clone expresses six virulence genes (out of 6 tested) - gyrB, toxR, flaA, vah1, angR and fur

BACTERIA CATALOGUE
Photobacterium damselae subs. damselae #A340

ORIGIN: Algarve, Portugal
HOST: Sparus aurata
TISSUE: Spleen
ANTIBIOTIC SENSITIVITY: TBA

BIOCHEMICAL CHARACTERIZATION: TBA
CULTURE MEDIUM:
TSA/TCBS
STORAGE DATE: 2023
MOLECULAR CHARACTERIZATION:
The clone was identified by PCR as Photobacterium damselae subs. damselae. The clone expresses six virulence genes (out of 9 tested) - toxR, gyrB, recA, fur, PLPV and HLYACH.

BACTERIA CATALOGUE
Vibrio fischeri #A179

ORIGIN:
Algarve, Portugal
HOST: Sparus aurata
TISSUE:
Kidney
ANTIBIOTIC SENSITIVITY: TBA
BIOCHEMICAL CHARACTERIZATION:
Colour: Yellow
Form: Bacilococus
Gram:Mobility: +
Oxidase: + Fermentation: -
Aminoacids (ODC/LDC/ADC): +/+/+
CULTURE MEDIUM:
TSA/TCBS
STORAGE DATE: 2023
MOLECULAR CHARACTERIZATION: The clone was identified by PCR as Vibrio fischeri


BACTERIA CATALOGUE
Photobacterium damselae subs. piscicida #A156

ORIGIN: Algarve, Portugal
HOST: Argyrosomus regius
TISSUE: Spleen
ANTIBIOTIC SENSITIVITY: TBA
BIOCHEMICAL CHARACTERIZATION: TBA
CULTURE MEDIUM:
TSA/Blood agar
STORAGE DATE: 2023
MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Photobacterium damselae subs. piscicida
The clone expresses seven virulence genes (out of 9 tested) - toxR, aip56, gyrB, recA, fur, PLPV and HLYACH.

BACTERIA CATALOGUE
Photobacterium damselae subs. piscicida #A008

ORIGIN: Algarve, Portugal
HOST: Sparus aurata
TISSUE: Spleen
ANTIBIOTIC SENSITIVITY:
OT, ENR, FFC and UB
MORTALITY: 0.1%/day
BIOCHEMICAL CHARACTERIZATION: TBA
CULTURE MEDIUM:
TSA/Blood agar
STORAGE DATE: 2023
MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Photobacterium damselae subs. piscicida
The clone expresses nine virulence genes (out of 9 tested) - toxR, aip56, irp2, frpA, gyrB, recA, fur, PLPV and HLYACH.

BACTERIA CATALOGUE
Photobacterium damselae subs. piscicida #A007

ORIGIN: Algarve, Portugal
HOST: Dicentrarchus labrax
TISSUE: Spleen
ANTIBIOTIC SENSITIVITY: ENR, and UB and OTC
MORTALITY: 1%/day
BIOCHEMICAL CHARACTERIZATION: TBA
CULTURE MEDIUM:
TSA/Blood agar
STORAGE DATE: 2023
MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Photobacterium damselae subs. piscicida.
The clone expresses seven virulence genes (out of 7 tested) - toxR, aip56, irp2, frpA, gyrB, recA, and fur.

Vibrio anguillarum #A062

ORIGIN: Algarve, Portugal
HOST: Oryzias latipes
TISSUE:
TBA
ANTIBIOTIC SENSITIVITY:
TBA
BIOCHEMICAL CHARACTERIZATION:
TBA
CULTURE MEDIUM:
TSA/TCBS
STORAGE DATE: 2021
MOLECULAR CHARACTERIZATION:
The clone was identified by PCR as Vibrio anguillarum.

The clone expresses six virulence genes (out of 6 tested) - gyrB, toxR, flaA, vah1, angR and fur.

Vibrio harveyi #A088

ORIGIN: Algarve, Portugal
HOST: Sardina pilchardus
TISSUE: TBA
ANTIBIOTIC SENSITIVITY:
ENR, and UB (OT resistant)
MORTALITY: 0.3%/day
BIOCHEMICAL CHARACTERIZATION:
Colour: Yellow
Gram:Mobility:Oxidase: +
CULTURE MEDIUM: TSA/TCBS
STORAGE DATE: 2021
MOLECULAR CHARACTERIZATION:
The clone was identified by PCR as Vibrio harveyi

The clone expresses six virulence genes (out of 6 tested) - topA, gyrB, toxR, flaA, recA and fur

BACTERIA CATALOGUE

ORIGIN: Algarve, Portugal
HOST: Argyrosomus regius
TISSUE: Liver
ANTIBIOTIC SENSITIVITY:
ENR, and UB (OT resistant)
MORTALITY: 13%/day
BIOCHEMICAL CHARACTERIZATION:
Colour: Yellow
Form: Bacilococus
Gram: -
Mobility: -
Oxidase: +
CULTURE MEDIUM:
TSA/Blood agar
STORAGE DATE: 2021
MOLECULAR CHARACTERIZATION:
The clone was identified by PCR as Vibrio harveyi.

The clone expresses six virulence genes (out of 6 tested) - topA, gyrB, toxR, flaA, recA and fur

BACTERIA CATALOGUE

ORIGIN:
Algarve, Portugal
HOST:
Solea senegalensis
TISSUE:
TBA
ANTIBIOTIC SENSITIVITY:
ENR, and UB (OT resistant)
MORTALITY:
21%/day

BIOCHEMICAL CHARACTERIZATION:
Colour: Yellow
Form: Bacilococus
CULTURE MEDIUM:
TSA/TCBS
STORAGE DATE: 2021
MOLECULAR CHARACTERIZATION:
The clone was identified by PCR as Vibrio harveyi
The clone expresses six virulence genes (out of 6 tested) - topA, gyrB, toxR, flaA, recA and fur.

BACTERIA CATALOGUE

ORIGIN:
Algarve, Portugal
HOST: Seriola dumerili
TISSUE: Spleen
ANTIBIOTIC SENSITIVITY: TBA
MORTALITY: 4%/day
BIOCHEMICAL CHARACTERIZATION:
Colour: Yellow
Form: Bacilococus
Gram:Mobility: +
Oxidase: + Fermentation: +
Aminoacids (ODC/LDC/ADC): +/+or-/+
CULTURE MEDIUM: TSA/TCBS
STORAGE DATE: 2021
MOLECULAR CHARACTERIZATION:
The clone was identified by PCR as Vibrio harveyi.

The clone expresses six virulence genes (out of 6 tested) - topA, gyrB, toxR, flaA, recA and fur.

BACTERIA CATALOGUE
Photobacterium damselae subs. piscicida #A213

ORIGIN: Algarve, Portugal
HOST: Dicentrarchus labrax
TISSUE: Spleen
ANTIBIOTIC SENSITIVITY: OT, ENR, UB
CULTURE MEDIUM: TBA
STORAGE DATE: 2023
MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Photobacterium damselae subs. piscicida.
The clone expresses nine virulence genes (out of 9 tested) - toxR, aip56, irp2, frpA, gyrB, recA, fur, PLPV, and HLYACH.
BACTERIA CATALOGUE
Vibrio anguillarum #A216



ORIGIN:
Algarve, Portugal
HOST:
Dicentrarchus labrax
TISSUE: Spleen
ANTIBIOTIC SENSITIVITY:
FM, FFC, ENR, OT

BIOCHEMICAL CHARACTERIZATION:
Colour: Yellow
Form: Rod-shape
Mobility: +
Oxidase: +
CULTURE MEDIUM: TBA
STORAGE DATE: 2023
MOLECULAR CHARACTERIZATION:
The clone was identified by PCR as Vibrio anguillarum.
The clone expresses six virulence genes (out of 6 tested) - GB, toxR, flaA, vah1, angR and fur.

BACTERIA CATALOGUE
Photobacterium damselae subs. piscicida #A228

ORIGIN: Algarve, Portugal
HOST: Sparus aurata
TISSUE: Liver
ANTIBIOTIC SENSITIVITY: TBA
CULTURE MEDIUM: TBA
STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION:
The clone was identified by PCR as Photobacterium damselae subs. piscicida
The clone expresses nine virulence genes (out of 9 tested) - toxR, aip56, irp2, frpA, gyrB, recA, fur, PLPV, and HLYACH

BACTERIA CATALOGUE
Photobacterium damselae subs. piscicida #A234

ORIGIN: Algarve, Portugal
HOST: Sparus aurata
TISSUE: Liver
ANTIBIOTIC SENSITIVITY:
TBA
MORTALITY: 1%
CULTURE MEDIUM:
TBA
STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION:
The clone was identified by PCR as Photobacterium damselae subs. piscicida
The clone expresses seven virulence genes (out of 9 tested) - toxR, aip56, irp2, frpA, gyrB, recA, and fur.









