PATHOGENIC BACTERIA CATALOGUE

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PATHOGENIC BACTERIA

CATALOGUE

In the scope of Biobanco Azul Português

BACTERIA CATALOGUE

ORIGIN:

Algarve, Portugal

HOST: Sparus aurata

TISSUE: Whole larvae

CULTURE MEDIUM: TSA/TCBS

ANTIBIOTIC SENSITIVITY: OT, ENR, FFC and UB

MORTALITY: 3%/day

BIOCHEMICAL CHARACTERIZATION: TBA

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Vibrio harveyi.

The clone expresses four virulence genes (out of 6 tested) – topA, flaA, recA and fur.

Vibrio harveyi #A183

ORIGIN: Algarve, Portugal

HOST: Dicentrarchus labrax

TISSUE: Kidney

CULTURE MEDIUM: TSA/TCBS

ANTIBIOTIC SENSITIVITY:

OT, ENR, UB and FFC

BIOCHEMICAL CHARACTERIZATION:

Colour: Yellow

Form: Bacilococus

Gram: -

Mobility: +

Oxidase: -/+

Fermentation: + Aminoacids (ODC/LDC/ADC): -/+/-

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Vibrio harveyi

The clone expresses six virulence genes (out of 6 tested) - topA, gyrB, toxR, flaA, recA and fur

Vibrio harveyi #A205

BACTERIA CATALOGUE

Aeromonas hydrophila #A165

ORIGIN: Algarve, Portugal

HOST: Danio rerio

TISSUE: Liver

ANTIBIOTIC SENSITIVITY: TBA

BIOCHEMICAL CHARACTERIZATION: TBA

CULTURE MEDIUM:

TSA/Basal Medium

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION: The clone was identified by PCR as Aeromonas hydrophila.

BACTERIA CATALOGUE

Photobacterium damselae subs. piscicida #A157

ORIGIN: Algarve, Portugal

HOST: Dicentrarchus labrax

TISSUE: Liver

ANTIBIOTIC SENSITIVITY:

OT, ENR, UB and FFC

BIOCHEMICAL CHARACTERIZATION: TBA

CULTURE MEDIUM:

TSA/Blood agar

STORAGE DATE: 2022

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Photobacterium damselae subs. piscicida.

The clone expresses seven virulence genes (out of 7 tested) - toxR, aip56, gyrB, recA, fur, irp2, and frpA.

BACTERIA CATALOGUE

ORIGIN:

Algarve, Portugal

HOST: Dicentrarchus labrax

TISSUE: Spleen

ANTIBIOTIC SENSITIVITY: OT, ENR and FMM

BIOCHEMICAL CHARACTERIZATION: TBA

CULTURE MEDIUM: TSA/TCBS

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Vibrio anguillarum.

The clone expresses six virulence genes (out of 6 tested) - gyrB, toxR, flaA, vah1, angR and fur

Vibrio anguillarum #A180

BACTERIA CATALOGUE

Photobacterium damselae subs. damselae #A340

ORIGIN: Algarve, Portugal

HOST: Sparus aurata

TISSUE: Spleen

ANTIBIOTIC SENSITIVITY: TBA

BIOCHEMICAL CHARACTERIZATION: TBA

CULTURE MEDIUM:

TSA/TCBS

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Photobacterium damselae subs. damselae. The clone expresses six virulence genes (out of 9 tested) - toxR, gyrB, recA, fur, PLPV and HLYACH.

BACTERIA CATALOGUE

Vibrio fischeri #A179

ORIGIN:

Algarve, Portugal

HOST: Sparus aurata

TISSUE:

Kidney

ANTIBIOTIC SENSITIVITY: TBA

BIOCHEMICAL CHARACTERIZATION:

Colour: Yellow

Form: Bacilococus

Gram:Mobility: +

Oxidase: + Fermentation: -

Aminoacids (ODC/LDC/ADC): +/+/+

CULTURE MEDIUM:

TSA/TCBS

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION: The clone was identified by PCR as Vibrio fischeri

BACTERIA CATALOGUE

Photobacterium damselae subs. piscicida #A156

ORIGIN: Algarve, Portugal

HOST: Argyrosomus regius

TISSUE: Spleen

ANTIBIOTIC SENSITIVITY: TBA

BIOCHEMICAL CHARACTERIZATION: TBA

CULTURE MEDIUM:

TSA/Blood agar

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Photobacterium damselae subs. piscicida

The clone expresses seven virulence genes (out of 9 tested) - toxR, aip56, gyrB, recA, fur, PLPV and HLYACH.

BACTERIA CATALOGUE

Photobacterium damselae subs. piscicida #A008

ORIGIN: Algarve, Portugal

HOST: Sparus aurata

TISSUE: Spleen

ANTIBIOTIC SENSITIVITY:

OT, ENR, FFC and UB

MORTALITY: 0.1%/day

BIOCHEMICAL CHARACTERIZATION: TBA

CULTURE MEDIUM:

TSA/Blood agar

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Photobacterium damselae subs. piscicida

The clone expresses nine virulence genes (out of 9 tested) - toxR, aip56, irp2, frpA, gyrB, recA, fur, PLPV and HLYACH.

BACTERIA CATALOGUE

Photobacterium damselae subs. piscicida #A007

ORIGIN: Algarve, Portugal

HOST: Dicentrarchus labrax

TISSUE: Spleen

ANTIBIOTIC SENSITIVITY: ENR, and UB and OTC

MORTALITY: 1%/day

BIOCHEMICAL CHARACTERIZATION: TBA

CULTURE MEDIUM:

TSA/Blood agar

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Photobacterium damselae subs. piscicida.

The clone expresses seven virulence genes (out of 7 tested) - toxR, aip56, irp2, frpA, gyrB, recA, and fur.

Vibrio anguillarum #A062

ORIGIN: Algarve, Portugal

HOST: Oryzias latipes

TISSUE:

TBA

ANTIBIOTIC SENSITIVITY:

TBA

BIOCHEMICAL CHARACTERIZATION:

TBA

CULTURE MEDIUM:

TSA/TCBS

STORAGE DATE: 2021

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Vibrio anguillarum.

The clone expresses six virulence genes (out of 6 tested) - gyrB, toxR, flaA, vah1, angR and fur.

Vibrio harveyi #A088

ORIGIN: Algarve, Portugal

HOST: Sardina pilchardus

TISSUE: TBA

ANTIBIOTIC SENSITIVITY:

ENR, and UB (OT resistant)

MORTALITY: 0.3%/day

BIOCHEMICAL CHARACTERIZATION:

Colour: Yellow

Gram:Mobility:Oxidase: +

CULTURE MEDIUM: TSA/TCBS

STORAGE DATE: 2021

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Vibrio harveyi

The clone expresses six virulence genes (out of 6 tested) - topA, gyrB, toxR, flaA, recA and fur

BACTERIA CATALOGUE

ORIGIN: Algarve, Portugal

HOST: Argyrosomus regius

TISSUE: Liver

ANTIBIOTIC SENSITIVITY:

ENR, and UB (OT resistant)

MORTALITY: 13%/day

BIOCHEMICAL CHARACTERIZATION:

Colour: Yellow

Form: Bacilococus

Gram: -

Mobility: -

Oxidase: +

CULTURE MEDIUM:

TSA/Blood agar

STORAGE DATE: 2021

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Vibrio harveyi.

The clone expresses six virulence genes (out of 6 tested) - topA, gyrB, toxR, flaA, recA and fur

Vibrio harveyi #A092

BACTERIA CATALOGUE

ORIGIN:

Algarve, Portugal

HOST:

Solea senegalensis

TISSUE:

TBA

ANTIBIOTIC SENSITIVITY:

ENR, and UB (OT resistant)

MORTALITY:

21%/day

BIOCHEMICAL CHARACTERIZATION:

Colour: Yellow

Form: Bacilococus

CULTURE MEDIUM:

TSA/TCBS

STORAGE DATE: 2021

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Vibrio harveyi

The clone expresses six virulence genes (out of 6 tested) - topA, gyrB, toxR, flaA, recA and fur.

Vibrio harveyi #A096

BACTERIA CATALOGUE

ORIGIN:

Algarve, Portugal

HOST: Seriola dumerili

TISSUE: Spleen

ANTIBIOTIC SENSITIVITY: TBA

MORTALITY: 4%/day

BIOCHEMICAL CHARACTERIZATION:

Colour: Yellow

Form: Bacilococus

Gram:Mobility: +

Oxidase: + Fermentation: +

Aminoacids (ODC/LDC/ADC): +/+or-/+

CULTURE MEDIUM: TSA/TCBS

STORAGE DATE: 2021

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Vibrio harveyi.

The clone expresses six virulence genes (out of 6 tested) - topA, gyrB, toxR, flaA, recA and fur.

Vibrio harveyi #A108

BACTERIA CATALOGUE

Photobacterium damselae subs. piscicida #A213

ORIGIN: Algarve, Portugal

HOST: Dicentrarchus labrax

TISSUE: Spleen

ANTIBIOTIC SENSITIVITY: OT, ENR, UB

CULTURE MEDIUM: TBA

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Photobacterium damselae subs. piscicida.

The clone expresses nine virulence genes (out of 9 tested) - toxR, aip56, irp2, frpA, gyrB, recA, fur, PLPV, and HLYACH.

BACTERIA CATALOGUE

Vibrio anguillarum #A216

ORIGIN:

Algarve, Portugal

HOST:

Dicentrarchus labrax

TISSUE: Spleen

ANTIBIOTIC SENSITIVITY:

FM, FFC, ENR, OT

BIOCHEMICAL CHARACTERIZATION:

Colour: Yellow

Form: Rod-shape

Mobility: +

Oxidase: +

CULTURE MEDIUM: TBA

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Vibrio anguillarum.

The clone expresses six virulence genes (out of 6 tested) - GB, toxR, flaA, vah1, angR and fur.

BACTERIA CATALOGUE

Photobacterium damselae subs. piscicida #A228

ORIGIN: Algarve, Portugal

HOST: Sparus aurata

TISSUE: Liver

ANTIBIOTIC SENSITIVITY: TBA

CULTURE MEDIUM: TBA

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Photobacterium damselae subs. piscicida

The clone expresses nine virulence genes (out of 9 tested) - toxR, aip56, irp2, frpA, gyrB, recA, fur, PLPV, and HLYACH

BACTERIA CATALOGUE

Photobacterium damselae subs. piscicida #A234

ORIGIN: Algarve, Portugal

HOST: Sparus aurata

TISSUE: Liver

ANTIBIOTIC SENSITIVITY:

TBA

MORTALITY: 1%

CULTURE MEDIUM:

TBA

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Photobacterium damselae subs. piscicida

The clone expresses seven virulence genes (out of 9 tested) - toxR, aip56, irp2, frpA, gyrB, recA, and fur.

BACTERIA CATALOGUE

Vibrio splendidus #A235

ORIGIN: Algarve, Portugal

HOST: Sparus aurata

TISSUE: Skin

ANTIBIOTIC SENSITIVITY: TBA

MORTALITY:

100 fish/day

CULTURE MEDIUM: TBA

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Photobacterium damselae subs. piscicida.

The clone expresses nine virulence genes (out of 9 tested) - toxR, aip56, irp2, frpA, gyrB, recA, fur, PLPV, and HLYACH.

BACTERIA CATALOGUE

Vibrio harveyi #A238

ORIGIN: Algarve, Portugal

HOST: Sparus aurata

TISSUE: Skin

ANTIBIOTIC SENSITIVITY:

OT, ENR, UB, FFC

MORTALITY:

100 fish/day

CULTURE MEDIUM: TBA

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Vibrio harveyi

The clone expresses four virulence genes (out of 6 tested) - topA, flaA, recA and fur

BACTERIA CATALOGUE

Vibrio anguillarum #A341

ORIGIN: Algarve, Portugal

HOST: Dicentrarchus labrax

TISSUE: Spleen

ANTIBIOTIC SENSITIVITY: ENR, FM, OT, FFC

CULTURE MEDIUM: TBA

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Vibrio anguillarum

The clone expresses six virulence genes (out of 6 tested) - gyrB, toxR, flaA, vah1, angR and fur.

BACTERIA CATALOGUE

Vibrio harveyi #A345

ORIGIN: Algarve, Portugal

HOST: Sparus aurata

TISSUE: Whole larvae

ANTIBIOTIC SENSITIVITY: FFC, ENR, UB, OT

CULTURE MEDIUM: TBA

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Vibrio harveyi.

The clone expresses six virulence genes (out of 6 tested) - topA, gyrB, toxR, flaA, recA and fur.

CATALOGUE

ORIGIN: Algarve, Portugal

HOST: Sparus aurata

TISSUE: Whole larvae

ANTIBIOTIC SENSITIVITY: FFC, ENR, OTC, UB

CULTURE MEDIUM: TBA

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION: The clone was identified by PCR as Vibrio splendidus.

BACTERIA CATALOGUE

Vibrio splendidus #A364

ORIGIN: Algarve, Portugal

HOST: Sparus aurata

TISSUE: Whole larvae

ANTIBIOTIC SENSITIVITY: TBA

CULTURE MEDIUM: TBA

STORAGE DATE: 2024

MOLECULAR CHARACTERIZATION: The clone was identified by PCR as Vibrio splendidus

BACTERIA CATALOGUE

ORIGIN:

Algarve, Portugal

HOST:

Solea senegalensis

TISSUE: Spleen

ANTIBIOTIC SENSITIVITY:

UB, ENR, FFC, OTC

MORTALITY: 0.04%

CULTURE MEDIUM: TBA

STORAGE DATE: 2024

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Vibrio harveyi

The clone expresses five virulence genes (out of 6 tested) - topA, gyrB, flaA, recA and fur

Vibrio Harveyi #A367

BACTERIA CATALOGUE

Vibrio mediterranei #A173

ORIGIN: Algarve, Portugal

HOST: Sparus aurata

TISSUE: Spleen

ANTIBIOTIC SENSITIVITY: TBA

CULTURE MEDIUM: TSA/TCBS

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION: The clone was identified by PCR as Vibrio mediterranei

Vibrio harvey #A222

ORIGIN: Algarve, Portugal

HOST: Dicentrarchus labrax

TISSUE: Liver

ANTIBIOTIC SENSITIVITY: FM, FFC, ENR (OT resistant)

CULTURE MEDIUM: TBA

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Vibrio harveyi.

The clone expresses five virulence genes (out of 6 tested) - topA, gyrB, flaA, recA and fur.

Photobact #A222

ORIGIN: Algarve, Portugal

HOST: Dicentrarchus labrax

TISSUE: Spleen

ANTIBIOTIC SENSITIVITY: OT, ENR, UB

CULTURE MEDIUM: TBA

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Photobacterium damselae subs. piscicida.

The clone expresses nine virulence genes (out of 9 tested) - toxR, aip56, irp2, frpA, gyrB, recA, fur, PLPV, and HLYACH.

BACTERIA CATALOGUE

Vibrio harveyi #A222

ORIGIN: Algarve, Portugal

HOST: Dicentrarchus labrax

TISSUE: Liver

ANTIBIOTIC SENSITIVITY: FM, FFC, ENR (OT resistant)

CULTURE MEDIUM: TBA

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Vibrio harveyi.

The clone expresses five virulence genes (out of 6 tested) - topA, gyrB, flaA, recA and fur

Photobact #A222

ORIGIN: Algarve, Portugal

HOST: Dicentrarchus labrax

TISSUE: Spleen

ANTIBIOTIC SENSITIVITY: OT, ENR, UB

CULTURE MEDIUM: TBA

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Photobacterium damselae subs. piscicida. The clone expresses nine virulence genes (out of 9 tested) - toxR, aip56, irp2, frpA, gyrB, recA, fur, PLPV, and HLYACH.

Photobact #A222

ORIGIN: Algarve, Portugal

HOST: Dicentrarchus labrax

TISSUE: Spleen

ANTIBIOTIC SENSITIVITY: OT, ENR, UB

CULTURE MEDIUM: TBA

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Photobacterium damselae subs. piscicida. The clone expresses nine virulence genes (out of 9 tested) - toxR, aip56, irp2, frpA, gyrB, recA, fur, PLPV, and HLYACH.

Photobact #A222

ORIGIN: Algarve, Portugal

HOST: Dicentrarchus labrax

TISSUE: Spleen

ANTIBIOTIC SENSITIVITY: OT, ENR, UB

CULTURE MEDIUM: TBA

STORAGE DATE: 2023

MOLECULAR CHARACTERIZATION:

The clone was identified by PCR as Photobacterium damselae subs. piscicida. The clone expresses nine virulence genes (out of 9 tested) - toxR, aip56, irp2, frpA, gyrB, recA, fur, PLPV, and HLYACH.

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