DR. LAURANNE DERMO KITS medical line

Dietro la tua bellezza .

Dermo Kits For transdermal devices

Info Manual Year 2014

Behind your Beauty .

Code: 4000 Name: Glyco Dermo-kit Contents ( commercial names ): snail secretion filtrate Contents ( INCI name ): snail secretion filtrate DESCRIPTION: The snail secretion is a natural source of: •

glycolic acid

•

aminoacids

•

proteins

•

peptides

•

Vitamins A, C and E

•

allantoin

•

trace elements ( copper, zinc, iron )

•

superoxide dismutase

•

lactic acid

•

elastin

•

collagene

•

folic acid

It is a superb soft-peeling and regenerating treatment, able to stimulate the proliferation of fibroblasts, accelerating the cellular turn-over, soliciting new fibers of elastin and collagen, for a global anti-ageing process.

Due to its richness in folic acid and glycolic acid, this treatment is also offering exciting results in the treatment of oily skin: it opens pores blocked by serum, renews and triggers the regeneration of skin damaged by acne.

SUITABLE TO: all skin types / all ages PROTOCOL OF USE: 1. apply one serum for each sitting, with the aid of a transdermal delivery device, or manually, with the aid of a brush. DO NOT WASH AWAY ( always refer to the device use manual before applying the product ) 2. Apply Neutralizing Cream 3. protect the skin with Photoprotecting Gel 4. Correct application protocol requires 2 applications/week 5. total treatment course: 5 weeks SIDE EFFECT: The treatment is a soft-peeling, thus a slight itching sensation may be felt during the process. The skin may get a temporary redness.

WARNINGS: In case of contact with eyes, rinse with water. Avoid to execute this treatment during summer season, as exposure to strong and direct sun rays may cause discomfort to the patient. Require the patient to avoid sun bathing, or visiting swinning pools ( because of chlorine in water ) during treatment.

INTERACTION WITH OTHER DR. LAURANNE PRODUCTS: None INTERACTION WITH OTHER SKIN PRODUCTS BRANDS: Unknown – liability towards eventual side-effects is declined PRECAUTIONS IN CASE OF PREGNANCY OR BABY-FEEDING: None.

FUNCTIONALITY OF MAIN INGREDIENTS: snail secretion filtrate.

Project: Bioactive compounds from garden snails Helix aspersa and their therapeutic and biological properties Research group Assoc. Prof. Dr. Dolashka-Angelova’s group has wide experience in studying the carbohydrate structure of glycoproteins, isolation, purification, and characterization of biologically active compounds (proteins, including enzymes, alkaloids, antibiotics). The structure and properties of the bioactive compounds from molluscan and arthropod an organisms is right now the main topic of this group. On the basis of these results, the polysaccharides structures and the gene sequence and hemocyanins isolated from garden snails Helix vulgaris, and marine snails Rapana venom were isolated.

The group has more that 90 publications with Impact factor -130 on this topic. Dolashka is an Editor in board of IDOSY, member to FEBS and representative member for Bulgaria in IUPAC. Assoc. Prof Dr. Dolashca—Angelova is coordinating several projects, sponsored by NATO (Brussels), the Bulgarian National Found for Scientific Researches and also collaborative projects with Germany (DFG and BMBF), Belgium and Italy, At the moment several projects are running: FWO (Belgium) - University of Gent on studies on gene sequence of hemocyanin isolated from garden snail Helix vulgaris and determination of carbohydrate structure using mass—spectroscopic techniques and methods; project with financial support from DFG (Germany) — studies on antiviraland imrnunomodulating properties of hemocyanins; financial support from CNR (Italy), to determine the quaternary structure of molluscan hemocyanins.

Several very well—equipped groups from Germany, Italy, Belgium, Greece, and Ukraine and from three hospitals are included in the research team: Prof Stenzl’s group in the laboratories of the Department of Urology, University of Tuebingen, Germany, Dr. Dimanov from hospital "Polimed”, Sofia and hospital for cancer diseases, Shumen, Bulgaria.

The main goal of the group is to isolate and characterize several compounds and extracts from garden snails Helica aspersa for cosmetic, medical and food industry.

Products from snail extract Snail extract for application in a powder form PRODUCT 1. (high protein, vitamin and amino acid content in combination with Aloe Vera) Designed for: increases and supports the immune systam, gives more energy.

PRODUCT 2. (protein with high quality, and vitamin and amino acid content) Designed for: improve the immune systam, preserve the lean muscle tissue in athletes and tone up their muscles.

PRODUCT 3. (as a food supplement) Designed for: to help prevent cardiac and cerebral vascular diseases, arteriosclerosis, anemia, reduces hunger by reducing insulin, restores virility and vitality and prolong life.

PRODUCT 4. (dietary product) Designed for: reduces hunger and weight loss, for patients with diabetes, for cases of ricket or bone problems.

PRODUCT 5. (medical product) Designed for: quickly renews the stomach and the digestive systam after antibiotics treatments or infectious diseases, for treatment of Stomach ulcer and asthma.

PRODUCT 6. (medical product) Designed for: anticancer therapy and for producing antibodies and as immunostimulator.

Snail extract for application in a cream form PRODUCT 1. (Face cream and lotion) Designed for: enrich the skin with the most important agents for the healthy skin and to support the skin turgor.

Product contains proteins, peptides, antibiotics, healthcare substances as allantoin with powerful emollient effect. It also contains glycolic acid, a variety of vitamins as A, C and E, minerals and trace elements including copper, zinc, and iron. Vitamin A also strengthens the cell membranes.

The glycolic acid provides gentle peeling of the skin, cleanses out the pores and kills bacteria causing acne. ln the same time this acid allows the other active substances as alantcin, collagen and ellastin to penetrate the skin.

They stimulate generation of new elastin, collagen and pigments in the cells. Collagen gives rigidity, resistance and firmness to the skin, contributing at the same time, to maintain smooth skin.

Designed for: enrich the skin with the most important agents for the healthy skin and to support the skin turgor.

PRODUCT 2. (Skin whitening cream) Designed for: to improve skin condition by increasing the dermis’ natural ability to take up and hold water.

Product contains high amount of superoxide dismutase and lactic acid. Superoxide dismutase is an antioxidant enzyme which protects cells form the free oxygen radicals and hydrogen peroxide.

Lactic acid and sodium lactate are substances produced by lactic acid bacteria which live in the snails. It has excellent moisturizing effect on the skin. The salt of lactic acid-the sodium lactate has the prope_rty to keep the skin moisture.

Designed for: to improve skin condition by increasing the dermis’ natural ability to take up and hold water.

PRODUCT 3. (Antianging treatment) Designed for: to stimulate the proliferation of fibroblasts, stimulation of new collagen and elastin fibers and and prevents the process of skin aging.

Product contains high amount of glycolic acid, amino acids, vitamins, collagen and elastin.

Glycolic acid provides generation of new collagen and elastin molecules in the cells and thus the wrinkles are filled and the scars from blemishes and burnings are covered and healed. The amino acids have a mild peeling effect and help the process of regeneration of the skin. Tyrosine is an amino acid which is commonly used in the sun care cosmetics. lt has a complex effect on the skin: hydrating and moisturising, due to hyaluronic acid, nourishing, due to regenerating and rejuvenating, due to soluble collagen, and Vitamin E. Designed for: to stimulate the proliferation of Hbroblasts, stimulation of new collagen and elastin fibers and a prevents the process of skin ageing. Remineralizes, fortifies, nourishes and improves skin's elasticity and firmness.

PRODUCT 4. (Acne treatment) Designed for: against bacteria, viruses and fungi, and protegtion against inflammations, opens the pores blocked by sebum, renews and triggers the regeneration of skin damaged by acne, injuries, overstretching, photo-aging or dermatological/medical treatments.

Product contains high amount of folic acid, specific antimicrobial and antibacterial peptides. lt is active against bacteria like E.co/L Staph. aureus, and other bacteria commonly causing acne and irritation of the skin. Folic acid opens the pores and helps the active substances to penetrate the skin.

PRODUCT 5. (Cream against stretch marks) Designed for: stretch mark removal by dissolving the scar tissues and rebuild healthy skin cells, also is recommended for use from the sixth pregnancy month for preventing the striaes.

PRODUCT 6. (for flesh, green wounds after operation and for diabetic people) Designed for: 1. to remove the skin spots and stains and formation of new skin. 2. ¡skin moisturising and elasticity by stimulating the secretion of collagen and elastin preventing the evolution of certain bacteria species which can be found in wounds.

References 1. Quevauviller A, Mainil J, Garcet S. Le mucus d’HéIix pomatia L. Preparation, composition, propriétés thérapeutiques et pharmacodynamiques. Rev Pathol Gen Comp 953;653:1514—38.

2. Pons F, Koenig M, Michelot R etal. L'effet bronchorelaxant de l'héIicidine,un extrait d'HéIix pomatia, fait intervenlr une liberation de prostagIandineE2. Pathol Biol 1999;47: 83-80.

3. Bruno Bonnemain, Helix and Drugs: Snails for Western Health Care From Antiquity to the Present.

Advance Access Publication 28 January 2005 eCAM 2005;2(1)25— 4. Webster L, Henderson R, Katz N, Ellis D. Characterization of confusion,an adverse event associated with intrathecal ziconotide infusion in chronic pain patients. Pain Med 2001;2:253—4.

5. Pathan, F.K., Venkata, D.A. and Panguluri, S.K. (2010) Recent patents on antimicrobial peptides. Recent Pat DNA Gene Seq. 1,10-6.

6. Peng, K., Kong, Y., Zhal, L., Wu, X., Jia, P., Liu, J.and Yu, H. (2009) Two novel antimicrobial peptides from centipede venoms. Toxicon. 2010 55 2-3, 274-279.

7. Hancock, R.E.W., Brown, K.L., Mookherjee, N., (2006) Host defence peptides from invertebrates — emerging antimicrobial strategies. lmmunobiol. 211, 315-322.

8. Chernish, S., Kim, S.I., Beckeer, G., Pleskach, V.A., Filatova, N.A., Anikin, V.B., Platonov, V.G., and Bulet, P. (2002) Antiviral and antitumor peptides from insects. Proceedings of the National Academy of Science USA. 99, 20, 12628-12632.

9.

Vesy Antwerp 10.

10. Moltedo, B., Faunes, F., Haussmann, D., De Ioannes, P., De Ioannes, A., Puente, J., Becker, lmmunotherapeutic Effect of Concholepas Hemocyanin in the Murine Bladder Cancer Model: Evidence for Conserved Antitumor Properties Among Hemccyanins, The J. of Urology 2006, 176, 6:2690-2695.

11. Becker Ml, Fuentes A, Del Campo M, Manubens A, Nova E, Oliva H, Faunes F, Valenzuela MA, Campos-Vallette M, Aliaga A, Ferreira J, De Ioannes AE, De loainnes P, Moltedo B. Immunodominant role of CCHA subunit of Concholepas hemocyanin is associated with unique biochemical properties. Int lmmunopharmacol. 2009, 9(3):330-339.

12. Chiarella P, Edelmann B, Fazio VM, et al. Antigenic features of protein carriers commonly used in immunisation trials. Biotechnol Lett. 2010, Apn 30.

13. P. Dolashka-Angelova, H. Schwarz, A. Dolashki, M. Beltramini, B. Salvato, M. Schick, M. Saeed and W. Voelter. "Oligomeric stability of Rapana venosa hemocyanin (RvH) and its structural subunits". Biochim.

Biophys. Acta 1646 (1-2) 77-85 (2003).

14. Aleksander Dolashki, Jtirgen Schiitz, Rumyana Hristova, Wolfgang Voelter and Pavlina DolashkaAngelova Spectroscopic properties of non-glycosilated functional unit KLH,- c of keyhole limpet hemocyanin" World J. of Agricul. Sciences 1 (2)129-136 (2005).

15. L. Velkova, I. Dimitrov, H. Schwarz, S. Stevanovic, W. Voelter, B. Salvato and P. Dolashka-Angelova.

Structure of hemocyanin from garden snail Helix vulgaris. Comp. Biochem. Physiology B: 157, 1, 16-25 (2010).

16. P. Dolashka-Angelova, T. Stefanova, E. Livaniou, L. Velkova, P. Klimentzou, S. Stevanovic, H. Neychev, H. Schwarz, W. Voelter. immunological potential of Helix vulgaris and Rapana venosa hemocyanins". immunologicalInvestigations, 37(8), 822-40 (2008).

17. Dolashka-Angelova P, Lieb B, Velkova L, Heilen N, Sandra K, Nikolaeva-Glomb L, Dolashki A, Galabov AS, Beeumen JV, Stevanovic S, Voelter W, Devreese B. Identification of glycosylated sites in Rapana hemocyanin by mass spectrometry and gene sequence, and their antiviral effect. Bioconjug Chem.

20(7):1315-22 (2009) 18. P. Dolashka-Angelova, L. Velkova, l. lliev, A. Beck, A. Dolashki, L.Yossifova, R. Toshkova, W. Voelter, and S. Zacharieva. Antitumor Activity of Glycosylated Molluscan Hemccyanins via Guerin ascites tumor.

Immunol. Investigation (2010) in press.

19. P. Dolashka-Angelova, L. Velkova, S. Shishkov, K. Kostova, A. Dolashki, l. Dimitrov, B. Atanasov, B. Devreese, W. Voelter and J. Van Beeumen Glycan structures of the structural subunit RvH2 of Rapana venosa hemocyanin and its effect on HSV type 1 virus. Carbohydrate research (2010) in press.

20. K. Sandra, P. Dolashka-Angelova, B. Devreese, J. Van Beeumen, New insights in Rapana venosa hemocyani N-glycosylation resulting from on-line mass spectrometric analyses. Glycobiology, 17(2):14156 (2007).

21. Nesterova, N. , Dolashka-Angelova, P. , Zagorodnya, S. , Moshtanska, V., Baranova, G., Golovan, A., Kurova, A., ln Vitro Investigation of Cytotoxic Action of Hemccyanins on Cell Cultures. Antiviral Research, 86 (1), p.A63-A63, 2010.

22. Yossifova L., lliev l., Petkova S., Dolashka-Angelova P., Mihov L. and Zacharieva S. lmunological research on the protective properties of a conjugate of total larval antigen with hemocyanin derived from He/ix vulgaris against infection with Trichinella spiralis. Biotech. biotech. Equip. 23(2), 597-600 (2009).

23. R. Toshkova, E. Ivanova, R. Hristova, W. Voelter P. Dolashka-Angelova, Effect of Rapana venosa Hemocyanin on Antibody-Dependent Cell Cytotoxicicity (ADCC) and Mitogen Responsibility of Lymphocytes from Hamsters with Progressing Myeloid Tumors. World J. of Medical Sciences 4 (2): 135142 (2009).

24. Nosanchuk J. D. and Casadevall A. (2003), The contribution of melanin to microbial pathogensis. Cell Microbiol. 5, 203-223.

25. Duran N. and Esposito E. (2000), Potential applications of oxidative enzymes and phenoloxidase-like compounds in wastewater and soil treatment: a review. Appl. Catal. B: Environ. 28, 83-99.

26. Dolashki, A., Gushterova, A., Voelter, W. and Tchorbanov, B. (2009) Purification and Characterization of Tyrosinases from Srreptomyccs olbus, Z Naturforsch. [C] pp.724-732 (64).

27. Dolashki, A., Gushterova, A., Voelter, W. and Tchorbanov B. (2009) Identification and characterization of tyrosinase from Streptomyces olbus by mass spectrometry. Biotechnol & Biotechnol. Equha. pp.946-950 (23).

SHELF LIFE: 3 years WARNING FOR STORAGE: store in a cool and dry place.

LAST REVISION: Jan. 2014 CONTACT

: www.drlauranne.eu

Code: 4004 Name: StamCell Dermo-kit Contents ( commercial names ): grape staminal cells; bilberry extract; guaranà extract.

Contents ( INCI name ): Vitis Vinifera Fruit Meristam Cell Culture; Vaccinium Myrtillus Fruit Extract; Guaranà extract.

DESCRIPTION: intensive regenerating solution for topical application via transdermal delivery devices. With high sun protection activity.

To be applied for rejuvenating treatment, especially suggested in follow up to: •

microdermo abrasion applications

•

IPL ( pulsed light ) treatments

•

chemical peelings

SUITABLE TO: all skin types / all ages PROTOCOL OF USE: 1. apply one serum for each sitting, with the aid of a transdermal delivery device, or manually, with the aid of a brush. DO NOT WASH AWAY ( always refer to the device use manual before applying the product ) 2. Correct application protocol requires 2 applications/week 3. total treatment course: 5 weeks SIDE EFFECT: None WARNING: In case of contact with eyes, rinse with water.

INTERACTION WITH OTHER DR. LAURANNE PRODUCTS: None INTERACTION WITH OTHER SKIN PRODUCTS BRANDS: Unknown – liability towards eventual side-effects is declined

PRECAUTIONS IN CASE OF PREGNANCY OR BABY-FEEDING: None

FUNCTIONALITY OF MAIN INGREDIENTS: vegetable staminal cells PART I : introduction Stam Cells Cells in our bodies are programmed for specific functions. A skin cell, a brain cell, and a liver cell all contain the same DNA, or set of genes.

However, each cell’s fate is determined by a set of epigenetic (able to change gene expression patterns) signals that come from inside it and from the surrounding cells as well.

These signals are like command tags attached to the DNA that switch certain genes on or off. This selective coding creates all of the different kinds of cells in our bodies, which are collectively known as differentiated (specialized) cells.

Although differentiated cells vary widely in purpose and appearance, they all have one thing in common: they all come with a built-in operational limit.

After so many divisions, they lose their ability to divide and must be replaced. This is where stam cells come in. Your body also produces other cells that contain no specific programming. These stam cells are “blank,” so your body can essentially “format” them any way it pleases. Two universal aspects shared by this type of cell are: (1) the ability to replenish itself through a process of self-renewal and (2) the capacity to produce a differentiated cell.

In animals and humans, two basic kinds of stam cells exist: embryonic and adult stam cells. Embryonic stam cells have the power to change into any differentiated cell type found anywhere in your body. Adult stam cells, on the other hand, are generally more limited. They can only evolve into the specific type of cell found in the tissue where they are located. The primary function of these adult stam cells is maintenance and repair. But certain adult stam cells found in nature retain the unlimited developmental potential that embryonic stam cells possess. These cells have become the main focus for an exciting new wave of regenerative medicine (repairing damaged or diseased tissues and organs using advanced techniques like stam cell therapy and tissue engineering).

The Role of Stam Cells in the Skin The basal (innermost) layer of the skin’s epidermis comprises two basic types of cells: (1) the slowly dividing epidermal stam cells (that represent about 2-7% of the basal cell population) and (2) their rapidly dividing offspring that supply new cells to replace those that are lost or dying. The slow self-renewal process of epidermal stam cells, however, creates a problem. Because each epidermal stam cell only lasts for a certain number of divisions, and because each division runs the risk of lethal DNA mutation, the epidermal stam cell

population can become depleted. When this happens, lost or dying skin cells begin to outnumber their replacements and the skin’s health and appearance start to decline.

So what can be done? Scientists turned to plants for the answer.

Planting a Seed of Hope Plants also have stam cells. Like humans, plant stam cells depend on epigenetic control and signals from surrounding cells for their development.

Unlike humans, however, each plant-derived adult stam cell possesses the ability to generate a whole new plant. Scientists have found a way to harness the power of plant stam cells by growing plant tissues in culture.

The technique is a relatively simple one. First, viable tissue is obtained from a source plant. This material is called an explant. Next, a small cut is made in the explant. New cells form on the surface of this cut in an attempt to heal the wound. This colorless mass of cells is called a callus. The slowly dividing cells of a callus are undifferentiated cells that lack the characteristics of normal plant cells. They are essentially unprogrammed and full of potential.

With this technology, it is theoretically possible to propagate any plant cell in a liquid culture, opening up a whole new realm of possibilities. This fact started scientists thinking—what would happen if an extract of genetically long-lived plant stam cell tissue was applied to human skin?

The first tests have been conducted with stam cells obtained from a special variety of apple, cultivated in a certain isolated area of rural Switzerland , which fruits could be stored for a greatly extended period of time.

In essence, it was the genetically modified, longer-living stam cells of this tannin-rich variety of apple, called the Uttwiler Spätlauber apple, which were responsible for its unique storage longevity. Scientists obtained an explant from the leaf of one of these trees to produce a special anti-aging stam cell extract.

The technology: In contrast to human cells, those from plants are totipotent, meaning that every cell is able to regenerate new organs or even the whole plant. Also, all plant cells can dedifferentiate and become stam cells.

A novel technology named PhytoCellTec™ was established, based on the wound-healing mechanism of plants.

Part of a plant is wounded to induce the formation of callus cells. This wound- ealing tissue consists of dedifferentiated cells which are stam cells. Callus cells are harvested and cultivated in suspension, a systam that enables large scale production.

This innovative technology offers the following advantages: • Possibility to cultivate cells of rare and endangered

plants while respecting the environment • Plant material completely free of environmental pollutants and pesticides • Constant concentrations of metabolites in the stam cells

The tests showed amazing results.

At a concentration of only 0.1%, an extract of Uttwiler Spätlauber stam cells stimulated the proliferation of human stam cells by an astounding 80%! Another in vitro experiment conducted by the scientists involved fibroblast cells. These are the most common of all cells in the connective tissue of the skin. They manufacture the collagen, glycosaminoglycans, reticular and elastic fibers, and glycoproteins that make up the extracellular matrix (connective tissues providing support to cells). Fibroblasts not only helpprovide a structural framework for the skin, they also play a critical role in wound healing.

In their experiment, the scientists treated fibroblast cells with hydrogen peroxide for two hours until the cells began to show classic signs of aging. In scientific terms, this means that several genes essential for cell proliferation and growth were significantly down-regulated. However, after incubating these cells for 144 hours in a 2% Uttwiler Spätlauber extract, this downregulation of genes was effectively neutralized, and in some cases, it was actually reversed! In addition, the scientists noted that the expression of an important antioxidant enzyme called heme oxigenase 1 was also stimulated. Finally, the scientists conducted a human study to determine the anti-wrinkle effectiveness of a special cream containing a 2% Uttwiler Spätlauber extract along with lecithin liposomes. This patent-pending ingredient (called PhytoCellTec™ Malus Domestica) was applied twice daily to the crow’s feet area of 20 participants. Wrinkle depth was reduced by an average of 8% after just two weeks, and by 15% after four weeks— thus reducing the signs of aging!

PART II : the systam applied to skin-care Protection of Stam Cells is Essential To summarise: Our skin contains stam cells which are located in the basal layer of the epidermis.Their main functions are to replenish and maintain the balance of cells within the skin as well as to regenerate damaged tissues. Epidermal stam cells are thus: • essential for the renewal and repair of the skin • the unique source of keratinocytes.

Therefore, their protection is of great importance.

UV radiation is responsible for 80 % of face aging. Despite the use of sun filters, toxins and oxidants are generated by UV in the skin. This affects the most sensitive cells such as the epidermal stam cells which are rare and essential. Thus, their protection against UV stress is of great importance.

The latest of tests conducted by scientists, was realising by using grape stam cells.

A rare grape cultivar of Gamay was selected. This cultivar, originating from the Burgundy region in France, is characterized by its red flesh and juice due to the extremely high anthocyanin content. Most red grape varieties have white flesh under their skin but in the case of the Gamay Teinturier Fréaux, anthocyanin pigments are also present in the pulp.

Anthocyanins are powerful antioxidants and free radical scavengers. They act as a “sunscreen“ by absorbing UV radiation. Their effectiveness is further enhanced by interaction with other metabolites substances present in the grape.

These grape stam cells contain special epigenetic factors and metabolites which are able to protect human skin stam cells against UV radiation.

This brand new ingredient, called PhytoCellTec™ Solar Vitis was shown to: • help the skin stam cells to maintain their stam cell characteristics • protect these precious cells against the stresses induced by UV, the main aging factor therefore addressing to • Protect skin stam cells against UV stress • Delay senescence of essential cells • Fight photo-aging • For a vital and healthy-looking skin References 1. Barthel R, Aberdam D. Epidermal stam cells. J Eur Acad Dermatol Venereol. 2005 Jul;19(4):405-13.

2. Aberdam D. Epidermal stam cell fate: what can we learn from embryonic stam cells? Cell Tissue Res. 2008 Jan;331(1):103-7.

3. Blanpain C, Fuchs E. Epidermal stam cells of the skin. Annu Rev Cell Dev Biol. 2006;22:339-73.

4. Byrne ME, Kidner CA, Martienssen RA. Plant stam cells: divergent pathways and common themes in shoots and roots. Curr Opin Genet Dev. 2003 Oct;13(5):551-7.

5. Schmid D, Schurch P, Belser E, Zülli F. Plant stam Extract for Cell Longevity of Skin and Hair. SOFW Journal. 2008;134(5):30-5.

6. Mibelle AG Biochemistry – Switzerland

SHELF LIFE: 3 years WARNING FOR STORAGE: store in a cool and dry place.

LAST REVISION: Jan. 2014 CONTACT

: www.drlauranne.eu

Code: 4003 Name: Jalu Dermo-kit Contents ( commercial names ): Hyaluronic Acid – Albumin Contents ( INCI name ): Sodium Hyaluronate - Albumin DESCRIPTION: Hyaluronic acid for topical application, to be used by means of a transdermal delivery device – suggested with RF device. Suitable to all skin type, especially recommended for lifting & antiage effect ( reduces wrinkles ).

SUITABLE TO: dry, aged and wrinkled skins PROTOCOL OF USE: 1. apply one serum for each sitting, with the aid of a transdermal delivery device, or manually, with the aid of a brush. DO NOT WASH AWAY ( always refer to the device use manual before applying the product ) 2. Correct application protocol requires 2 applications/week 3. total treatment course: 5 weeks

SIDE EFFECT: None. An eventual stretching feeling is normal and is due to the Ha filling properties.

WARNINGS: In case of contact with eyes, rinse with water.

INTERACTION WITH OTHER DR. LAURANNE PRODUCTS: None INTERACTION WITH OTHER SKIN PRODUCTS BRANDS: Unknown – liability towards eventual side-effects is declined PRECAUTIONS IN CASE OF PREGNANCY OR BABY-FEEDING: None.

FUNCTIONALITY OF MAIN INGREDIENTS: Hyaluronic Acid ABSTRACT:

Aged skin has been shown to be characterized by reduced levels of hyaluronic acid (HA) and elevated levels of chondroitin sulphate proteoglycans. Such patterns, intriguingly, are also observed in scars. HA is found in young skin at the periphery of collagen and elastin fibres and where these types of fibres intersect. In aged skin, such connections with HA disappear. It is possible that the decreases in HA levels, which contribute to its disassociation with collagen and elastin as well as reduced water binding, may be involved in the changes noted in aged skin, including wrinkling, altered elasticity, reduced turgidity and diminished capacity to support the microvasculature of the skin.

As one of the primary GAGs, HA can bind 1000 times its weight in water, and may help the skin retain and maintain water. It is found in all connective tissue and is produced mainly by fibroblasts and keratinocytes in the skin. HA is localized not only in the dermis but also in the epidermal intercellular spaces, especially the middle spinous layer, but not in the stratum corneum (SC) or stratum granulosum. Aged skin, which is less plump than youthful skin, is characterized by decreased levels of HA. HYALURONANS: Hyaluronans have become the most popular agents used for soft tissue augmentation in the entire facial area. They are bio degradable, non permanent and have a remarkable safety profile. Their natural bio compatibility means that no pretreatment skin test is necessary. The soft pliable enhancement of natural tissue turgidity and contour makes them very acceptable in the lips and peri-oral / periocular regions. The more viscous agents are very helpful in the treatment of facial lipoatrophy particularly in the region of the cheeks and chin.

AVAILABLE PRODUCTS: Today, there are hyaluronic acid gels derived from both animals and bacteria available. Prior to use each hyaluronic acid gel is stabilized. The stabilization process varies according to manufacturer and explains differences in product longevity and viscosity. Today's trend is towards non-animal, stabilized hyaluronic acid (NASHA) derivatives that are produced by fermentation from cultured Streptococcus bacteria. This process eliminates any risk of Transmissible Spongiform Encephalopathy (TSE) contamination.

TOPICAL APPLICATION OF HYALURONIC ACID: The topical hyaluronic acid creams function in a different way then the injectable hyaluronic acid. The topical

form is applied to the skin and act as a super moisturizer that hydrate the skin and plump it. Another thicker form of hyaluronic acid in mask form is used to attract more water from the air.

Serums are concentrated, condensed, super charged supplements for daily skin care. They are able to deliver nutrients and actives deeper into the epidermis than treatment creams or lotions, therefore resulting in more pronounced results.

Retention of the essential moisture in skin layers results in combating wrinkling and reducing fine lines caused by dehydration.

References 1.

Duranti F, Salti G, Bovani B, et al. Injectable hyaluronic acid gel for soft tissue augmentation: A clinical and histological study.

Dermatol Surg. 1998;24:1317-1325.

2.

3.

Brown LH, Frank PJ. What's new in fillers? J Drugs Dermatol. 2003;2:250-253.

Klein AW. Skin filling. Collagen and other injectables of the skin. Dermatol Clin. 2001;19:491508.

4. Naoum C, Dasiou-Plakida D. Dermal filler materials and botulin toxin. Int J Dermatol. 2001;40:609-621.

5. Jordan D. Soft-tissue fillers for wrinkles, folds and volume augmentation. Can J Ophthalmol. 2003;38:285-288.

6. Lowe NJ, Maxwell CA, Lowe P, et al. Hyaluronic acid skin fillers: Adverse reactions and skin testing. J Am Acad Dermatol.

2001;45:930-933.

7. Micheels P. Human anti-hyaluronic acid antibodies: Is it possible? Dermatol Surg. 2001;27:185-191.

8. Friedman PM, Mafong EA, Kauvar AN, et al. Safety data of injectable nonanimal stabilized hyaluronic acid gel for soft tissue augmentation. Dermatol Surg. 2002;28:491-494.

9. Andre P. Hyaluronic acid and its use as a "rejuvenation" agent in cosmetic dermatology. Semin Cutan Med Surg. 2004;23:218-222.

10. Dover JS, Carruthers A, Carruthers J, et al. Clinical use of Restylane. Skin Therapy Lett. 2005;10:5-7.

11. Carruthers J, Carruthers A, Maberley D. Deep resting glabellar rhytides respond to BTX-A and Hylan B. Dermatol Surg.

2003;29:539-544.

12. Carruthers J, Carruthers A. A prospective, randomized, parallel group study analyzing the effect of BTX-A (Botox) and nonanimal sourced hyaluronic acid (NASHA, Restylane) in combination compared with NASHA (Restylane) alone in severe glabellar rhytides in adult female subjects: treatment of severe glabellar rhytides with a hyaluronic acid derivative compared with the derivative and BTX-A. Dermatol Surg. 2003;29:802-809.

13. Bosniak S, Cantisano-Zilkha M, Glavas IP. Nonanimal stabilized hyaluronic acid for lip augmentation and facial rhytid ablation. Arch Facial Plast Surg. 2004;6:379-383.

14. Carruthers J, Klein AW, Carruthers A, et al. Safety and efficacy of nonanimal stabilized hyaluronic acid for improvement of mouth corners. Dermatol Surg. 2005;31:276-280.

15. Narins RS, Brandt F, Leyden J, et al. A randomized, double-blind, multicenter comparison of the efficacy and tolerability of Restylane versus Zyplast for the correction of nasolabial folds. Dermatol Surg. 2003;29:588-595.

16. Carruthers A, Carey W, Dc Lorenzi C, et al. Randomized, double-blind comparison of the efficacy of two hyaluronic acid derivatives, restylane perlane and hylaform, in the treatment of nasolabial folds. Dermatol Surg. 2005;31(11 pt 2):1591-1598.

17. Rao J, Chi GC, Goldman MP. Clinical comparison between two hyaluronic acid-derived fillers in the treatment of nasolabial folds: hylaform versus restylane. Dermatol Surg. 2005;31(11 pt 2): 1587-1590.

18. Baumann LS, Shamban AT, Lupo MP, et al. Comparison of a family of new generation hyaluronic-acid based fillers with crosslinked bovine collagen in the correction of nasolabial folds: A double-masked, multicenter, randomized, within-subject study. Presented at: Annual Meeting of the American Academy of Dermatology; March 3-7, 2006; San Francisco, CA.

SHELF LIFE: 3 years WARNING FOR STORAGE: store in a cool and dry place.

LAST REVISION: Jan. 2014 CONTACT

: www.drlauranne.eu

Code: 4001 Name: Peptides Dermo-kit Contents ( commercial names ): 8-peptides Contents ( INCI name ): Water (aqua), Acetyl Octopeptide-3, Caprylyc Glycol DESCRIPTION: 8-Peptide ( combination of 8 amino acids which are linked together to create one particular peptide) able to reduce expression wrinkles topically, with an alternative mechanism to Botulin Toxin.

SUITABLE TO: normal skins with wrinkles, aged skins PROTOCOL OF USE: 4. apply one serum for each sitting, with the aid of a transdermal delivery device, or manually, with the aid of a brush. DO NOT WASH AWAY ( always refer to the device use manual before applying the product ) 5. Correct application protocol requires 2 applications/week 6. total treatment course: 5 weeks

SIDE EFFECT: None.

WARNINGS: In case of contact with eyes, rinse with water.

INTERACTION WITH OTHER DR. LAURANNE PRODUCTS: None INTERACTION WITH OTHER SKIN PRODUCTS BRANDS: Unknown – liability towards eventual side-effects is declined PRECAUTIONS IN CASE OF PREGNANCY OR BABY-FEEDING: None.

FUNCTIONALITY OF MAIN INGREDIENTS: 8-Peptide ABSTRACT: Muscles responsible for the facial expression are contracted when they receive neurotransmitters released at the neuromuscular synapsis. An over-stimulation of the facial muscles, due to an excess of these chemical signals, leads to a greater skin strain. After the age of 30, first wrinkles start to appear as a consequence of such muscle contractions.

The study of the basic biochemical mechanism of anti-wrinkle activity led to the revolutionary world of peptides for topical application, which compete with the native protein for a position in the SNARE complex, essential for the muscle contraction and represent a gentle alternative to Botulin or other injectables.

8-peptide blocks the formation of the SNARE complex, with a potent anti-wrinkle activity. It also inhibits glutamate release in a dose-dependent manner.

ANTI-WRINKLE EFFICACY EVALUATION: Skin topography analysis were performed to measure the effectiveness of a cream containing 8-Peptides, applied twice per day. The maximum reduction value of wrinkles depth was 63% ( ref. Lipotec Group www.lipotec.com )

SHELF LIFE: 3 years WARNING FOR STORAGE: store in a cool and dry place.

LAST REVISION: Jan. 2014 CONTACT

: www.drlauranne.eu

Code: 4004 Name: Whitening Dermo-kit Contents ( commercial names ): ascorbic acid Contents ( INCI name ): 3-O-Ethyl Ascorbic Acid DESCRIPTION: This product is one kind of Vitamin C derivative. It can easy penetrate horniness and reach the dermis, then the enzyme decompounds them and releases the Vitamin C. It also can effectively inhibit the melanin formation by restrain the Cu2+ activity of tyrosinase, inhibit dermatitis and improve the skin color and elasticity.

SUITABLE TO: skins with pigmentary anomalies PROTOCOL OF USE: 7. apply one serum for each sitting, with the aid of a transdermal delivery device, or manually, with the aid of a brush. DO NOT WASH AWAY ( always refer to the device use manual before applying the product ) 8. Correct application protocol requires 2 applications/week 9. total treatment course: 5 weeks

SIDE EFFECT: None.

WARNINGS: In case of contact with eyes, rinse with water.

INTERACTION WITH OTHER DR. LAURANNE PRODUCTS: None INTERACTION WITH OTHER SKIN PRODUCTS BRANDS: Unknown – liability towards eventual side-effects is declined PRECAUTIONS IN CASE OF PREGNANCY OR BABY-FEEDING: None.

FUNCTIONALITY OF MAIN INGREDIENTS: ascorbic acid ABSTRACT: 3-O-ethyl ascorbic acid is an ethered derivative of ascorbic acid ，the most excellent derivative of ascorbic acid so far. It is very stable in chemical structure, a real stable and discolored derivative of ascorbic acid, but also it can enter into skin and be metabolized by body as ascorbic acid. So its effect is better than pure ascorbic acid.

The character of 3-O-ethyl ascorbic acid: •

Excellent whitening effect: inhibit the activity of Tyrosinase by act on Cu2+

•

prevent the synthesis of melanin（≥2%）

•

High antioxidation

•

Stable derivative of ascorbic acid

•

Lipophilic and hydrophilic structure

•

Anti-inflammation, inhibit the growth of bacteria

•

Improve the complexion, enhance the elasticity of skin.

•

Repair skin cells, accelerate the synthesis of collagen.

Inhibition of the activity of Tyrosinase

Reduction of melanin

whitening

References: 1. Briganti, S.; Camera, E.; Picardo, M. Pigment Cell Res. 2003, 16,101.

2. Rescigno, A.; Sollai, F.; Pisu, B.; Rinaldi, A.; Sanjust, E. J.Enzym. Inhib. Med. Chem.

2002, 17, 207.

3. Dooley, T. P. J. Dermatol. Treat. 1997, 8, 275.

4. Guevara, I. L.; Pandya, A. G. Int. J. Dermatol. 2001, 40, 212.

5. Nguyen, Q. H.; Bui, T. P. Int. J. Dermatol. 1995, 34, 75.

6. Okan, G.; Baykal, C. J. Eur. Acad. Dermatol. Venereol. 1999, 13,218.

7. Maeda, K.; Fukuda, M. J. Pharmacol. Exp. Ther. 1996, 276, 765.

8. Kong, K. H.; Park, S. Y.; Hong, M. P.; Cho, S. H. Comp. Biochem.Physiol. 2000, 125, 563.

9. Wittbjer, A.; Dahlback, B.; Odh, G.; Rosengren, A. M.;Rosengren, E.; Rorsman, H. Acta Derm. Venereol. (Stockh) 1989,69, 125.

10. Fling, M.; Horowitz, N. H.; Henemann, S. F. J. Biol. Chem. 1963,238, 2045.

11. Park, H. J.; Koh, J. U.; Ahn, S. Y.; Kong, K. H. Bull. KoreanChem. Soc. 2005, 26, 433.

12. Kang, S. J.; Choi, J. D. Korean Biochem. J. 1993, 26, 632.

13. Zhou, P.; Smith, N. L.; Lee, C. Y. J. Agric. Food Chem. 1993, 41,532.

14. Kwon, B. S.; Haq, A. K.; Pomerantz, S. H.; Halaban, R. Proc.Natl. Acad. Sci. U.S.A. 1987, 84, 7473.

15 Sang-Hoon Jeon et al: Inhibitory Effects on L-Dopa Oxidation of Tyrosinase by Skin-whitening Agents. Bull. Korean Chem. Soc. 2005, Vol. 26, No. 7 1135

SHELF LIFE: 3 years WARNING FOR STORAGE: store in a cool and dry place. Avoid direct light sources.

LAST REVISION: Jan. 2014 CONTACT

: www.drlauranne.eu

Code: 4005 Name: Pro-Lypo Dermo-kit Contents ( commercial names ): seaweeds complex Contents ( INCI name ): DESCRIPTION:

SUITABLE TO: cellulite treatments, applicable ona ll skin types, even the most sensitive.

PROTOCOL OF USE: 10. apply one serum for each sitting, with the aid of a transdermal delivery device, or manually, with the aid of a brush. DO NOT WASH AWAY ( always refer to the device use manual before applying the product ) 11. Correct application protocol requires 2 applications/week 12. total treatment course: 5 weeks

SIDE EFFECT: None.

WARNINGS: In case of contact with eyes, rinse with water.

INTERACTION WITH OTHER DR. LAURANNE PRODUCTS: None INTERACTION WITH OTHER SKIN PRODUCTS BRANDS: Unknown – liability towards eventual side-effects is declined PRECAUTIONS IN CASE OF PREGNANCY OR BABY-FEEDING: None.

FUNCTIONALITY OF MAIN INGREDIENTS: seaweeds ABSTRACT: Our Bodies, Our Aquariums?

The sea, as every science student knows, covers two-thirds of our planet. It is a remarkable place because it contains an harmonious balance of every element needed to support life at the cellular level. The elements of sea water, particularly trace elements, can affect our metabolic levels.

Like the planet, our bodies are made up of two-thirds water. In our bodies, water is an essential substance for the proper functioning of all body systems. In skin care, it presents constant problems because it escapes through almost all the cell membranes. The average person looses two or three litres of water per day as a result of normal body functions, thus leaving the skin dehydrated. Water is also needed to remove waste from the body.

There are many similarities between the sea and our bodies. French biologist Rene Quinton actually discovered that human white blood cells placed in sea water continue to live, although they die in all other artificial environments. Researchers continue to point out the almost uncanny similarity between sea water and human plasma, our bodies' intracellular fluid. "In the internal environment of our system and only there," wrote Quinton in Sea Water Organic Substance, "do we find the same mineral make-up, the same physiognomy, as that of sea water."

In essence, our bodies are marine aquariums where cells continue to live in the same condition as in their original home millions of years ago.

The sea, which is our nourishment, is also our healer, one biologist suggests. We don't know all its workings, its details or its secrets. But the sea knows better than anyone what we are made of - and what is missing when our bodies aren't functioning as they should.

So, why using just seaweed among all marine derivates? Because seaweed is a concentration of seawater.

As already said, seaweed, like seawater, is similar to human plasma.

When in 1897 the French doctor Rene Quinton demonstrated that human white blood globules continue to thrive in seawater, it proved that seaweed serves as an ideal medium to deliver all the nutritive benefits from the sea to the skin.

I ORIGIN - HISTORY The first form of life was a unicellular seaweed, which appeared in the ocean over 3 billion years ago. There are over 20,000 different species of seaweed present world-wide. Over 800 species of marine seaweed are found off the Brittany coast of France. The annual production of seaweed in this area is over 25 million tons.

II CLASSIFICATION •A. Blue Seaweeds Microscopic and unicellular i.e. Spirulina •B. Green Seaweeds 10 to 50 cm. These thrive at the upper section the sea i.e. Fucus •C. Brown Seaweeds10 cm to 16 meters. They thrive at the middle section of the sea. This is the category richest in amino acids, vitamins and minerals. Non-toxic to the living cell; it helps to intercept the aging process. Brown seaweed can reproduce as much as 60 feet in 10 months.

•D. Red Seaweeds10 cm to 40 cm. Thrive at the deepest levels of the sea. i.e. Desselaria Sanguinea

III HABITAT Seaweed grows in the sea, never deeper than 200 feet or 61 meters. Seaweed must be exposed to light in order to synthesize organic substances. The colour is determined by the intensity of light it receives. The seaweed plant has no roots, so it feeds by osmosis absorbing minerals and vitamins from the sea. Seaweed is simply a concentration of seawater.

IV HARVEST The harvesting of seaweed has been under strict regulation by the French government since 1852. There are three different methods of harvest: •A. Wreck Seaweed This seaweed is rejected from the sea and found washed up on the shore. It is used for agricultural purposes such as fertilizers. •B. Shore Seaweed These seaweeds are cut manually from rocks.

•C. Deep Sea Seaweed Harvested by divers and crews on fishing boats who are licensed to perform this task. The seaweed obtained from this type of harvesting is of the highest quality, rich in vitamins, minerals, trace elements and polysaccharides. Each harvest yields about 10 tons of seaweed.

V MANUFACTURE/TRANSFORMATION •A. Sun Drying and Oven Drying This method is done at very high temperatures of 450 degrees centigrade.

Then, by the use of ultrasound, the seaweed is micro-burst into a brown powder and alcoholic extractions are made. These methods yield a product which contains trace elements, but is deficient in vitamins and amino acids. •B. CRYOBROYAGE or cry crushing. Bursting of seaweed into particles of 100 microns at - 50 degrees centigrade. Followed by: •Lamination: Seaweed is further crushed into particles of 6 to 10 microns. •Homogenising: Done at a slow speed at room temperature.

•C. Ultra filtration and Centrifugation This process is done under sterile conditions. It yields a seaweed concentrate used in creams, lotions and serums.

VI BENEFITS The benefits of seaweed products to the skin are: Rebalancing, Nourishing, Hydrating, Cell Regenerating, Detoxifying, Purifying, Oxygenating, Remineralizing This is achieved due to the concentration of the following elements: Mineral Salts The mineral salts, particularly magnesium, potassium, calcium and silicium, act on the cells' vitality with their ionic potential. This prevents the cell from shrinking by providing a moisture balance.

Amino Acids: Cystine, Lysine, Aspartic, Glutamic, Proline, Methionine,Tryptophane, Arginine, Ornithine.

The amino acids act by nourishing and regenerating the fibroblast cell which stimulates the production of collagen and elastin.

Vitamins A, C, B1, B2, B 12, E, PP, K, D. Vitamins are essential for overcoming deficiencies which can result in: •broken capillaries •loss of firmness •skin lesions •dry, scaly skin.

Trace Elements: Iodine, Iron, Manganese, Boron, Cobalt. Copper, Zinc, Bromium, Nickel, Strontium, Aluminum, Barium, Titanium, Arsenic, Silver, Gold, Chromium, Fluorine, Tin. The multiple trace elements become catalysts which allow an increase in the speed of biological reactions for maximum skin penetration.

Seaweed based products, have the ability to stimulate circulation and the flow of oxygen to the deeper layers of the skin which results in the exchange of substances through: • Elimination of toxins • Increase in the flow of nutrients The ideal temperature for greater transdermal penetration is 98 degrees to 100 degrees Fahrenheit. Skin, sometimes referred to as the "third lung," is an organ of exchange, and seaweed products facilitate the exchange of substances, resulting in the purification and nourishment of the body.

We recommend seaweed-based treatments for relief of: 

Circulatory problems



Cellulite



Oedemas



Or just as a maintenance care for body shaping.

How do seaweed interact with our body?

Seaweeds can perform different actions on our body as they are available to release marine elements by osmosis.

Osmosis is the passage of water from a region of high water concentration, through a semi-permeable membrane, to a region of low water concentration.

Semi-permeable membranes are very thin layers of material ( cell membranes are semi-permeable ) which allow some things to pass through them but prevent other things from passing through.

Cell membranes will allow small molecules like oxygen, water, amino-acids, glucose, etc. to pass through, but will not allow the passage of larger molecules such as sucrose, starch, proteins, etc. Thus, they play a double-role in our body.

How come that the same ingredient ( SEAWEED ) is used for miscellaneous cosmetic purposes ( moisturizing, slimming, etc. ) ?

When you put a cell into a liquid containing water, one of the two following things will happen: 1. if the medium surrounding the cell has a higher water concentration than the cell, the cell will gain water by osmosis ( moisturizing action ).

2. if the medium has a lower concentration of water than the cell, the cell will loose water by osmosis.

Water crosses the cell membranes in both directions, but this time more water leaves the cell than enters it. Therefore, the cell will shrink ( reducing action ).

References: www.oceanplasma.org

SHELF LIFE: 3 years WARNING FOR STORAGE: store in a cool and dry place. Avoid direct light sources.

LAST REVISION: Jan. 2014 CONTACT

: www.drlauranne.eu