SADRŽAJ:
1. UVOD__________________________________________________________________4 1.1. Polifenolna jedinjenja____________________________________________________4 Fenolske kiseline____________________________________________________________7 Klorogenska kiselina________________________________________________________10 Ruzmarinska kiselina________________________________________________________11 Galna kiselina______________________________________________________________12 Kafena kiselina_____________________________________________________________12 1.2. Heterozidi (glukozidi, glikozidi)____________________________________________13 Kemijske osobine heterozida__________________________________________________16 Flavonoidni heterozidi_______________________________________________________16 Fizičko – kemijske osobine flavonoida__________________________________________19 Rutin_____________________________________________________________________20 1.3. Kromatografija_________________________________________________________22 Visokotlačna tekućinska kromatografija (HPLC)__________________________________22 Tipovi HPLC_____________________________________________________________24 Komponente uređaja za HPLC________________________________________________25 Detektori u HPLC sistemu____________________________________________________27 Elektrokemijski detektori (ED)________________________________________________27 2. CILJ RADA_____________________________________________________________28 3. MATERIJAL I METODE _________________________________________________29 Biljni materijal_____________________________________________________________29
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