Références sur les Plantes

bacterial multiplication, reduced severity of necrosis symptoms caused by the pathogen, and an increased defense response in the RAP2.6L-discrupted mutants compared to the wild-type plants. These changes accompanied DC3000-induced and RAP2.6L-dependent expression of several defense-related genes.

URL: http://www.sciencedirect.com/science/article/B6WPC-4YV82JW1/2/f0c7bc17cba17e76ff79afc4c0e15b3c Author Address: Ministry of Agriculture Key Laboratory of Monitoring and Management of Crop Pathogens and Pest Insects, Nanjing Agricultural University, Nanjing 210095, China XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Author: Sun W, Duan M, F Li, D-F Shu, S Yang, Q-W Meng, Year: 2010 Title: * Overexpression of tomato tAPX gene in tobacco improves tolerance to high or low temperature stress.

Secondary Title: Biologia Plantarum 54, 4, 614-620.

Publisher: Springer Netherlands Date: 2010-12-01 ISBN/ISSN: 0006-3134 Label: ReEn Physiol Keywords: Biomedical and Life Sciences - Lycopersicum esculentum - malondialdehyde - Nicotiana tabacum - photosystem 2 - thylakoid-bound ascorbate peroxidase - transgenic tobacco Abstract: In order to investigate the function of chloroplast ascorbate peroxidase under temperature stress, the thylakoid-bound ascorbate peroxidase gene from tomato leaf (TtAPX) was introduced into tobacco.

Transformants were selected for their ability to grow on medium containing kanamycin. RNA gel blot analysis confirmed that TtAPX in tomato was induced by chilling or heat stress. Over-expression of TtAPX in tobacco improved seed germination under temperature stress. Two transgenic tobacco lines showed higher ascorbate peroxidase activity, accumulated less hydrogen peroxide and malondialdehyde than wild type plants under stress condition. The photochemical efficiency of photosystem 2 in the transgenic lines was distinctly higher than that of wild type plants under chilling and heat stresses. Results indicated that the over-expression of TtAPX enhanced tolerance to temperature stress in transgenic tobacco plants.

URL: http://dx.doi.org/10.1007/s10535-010-0111-2 Author Address: College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Taiwan, 271018, P.R. China XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Author: Sun Zhao-Nan, Guo-Hua Yin, Yun-Zhi Song, Hai-Long An, Chang-Xiang Zhu and Fu-Jiang Wen, Year: 2010 Title: * Bacterially Expressed Double-Stranded RNAs against Hot-Spot Sequences of Tobacco Mosaic Virus or Potato Virus Y Genome Have Different Ability to Protect Tobacco from Viral Infection.

Secondary Title: Applied Biochemistry and Biotechnology Volume 162, Number 7, 1901-1914, DOI: 10.1007/s12010-010-8968-2 Publisher: Humana Press Inc. Date: 2010-11-01 ISBN/ISSN: 0273-2289 Label: ViRe Expression Bioengineering Keywords: Biomedical and Life Sciences - Tobacco mosaic virus - Potato virus Y - RNA interference hpRNAs - Hot-spot sequences Abstract: Posttranscriptional gene silencing, also known as RNA interference, involves degradation of homologous mRNA sequences in organisms. In plants, posttranscriptional gene silencing is part of a defense mechanism against virus infection, and double-stranded RNA is the pivotal factor that induces gene silencing.

In this paper, we got seven hairpin RNAs (hpRNAs) constructs against different hot-spot sequences of Tobacco mosaic virus (TMV) or Potato virus Y (PVY) genome. After expression in Escherichia coli HT115, we extracted the seven hpRNAs for the test in tobacco against TMV or PVY infection. The data suggest that different hpRNAs against different hot-spot sequences of TMV or PVY genome had different ability to protect tobacco plants from viral infection. The resistance to TMV conferred by the hpRNA against the TMV movement protein was stronger than other TMV hpRNAs; the resistance to PVY conferred by the hpRNA