Références sur les Plantes

Author Address: Indian Institute of Technology Roorkee, Roorkee 247 667, Uttarakhand, India.

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Author: Marti L, Stefano G, Tamura K, Hawes C, Renna L, Held MA, Brandizzi F, Year: 2010 Title: * A missense mutation in the vacuolar protein GOLD36 causes organizational defects in the ER and aberrant protein trafficking in the plant secretory pathway.

Journal: The Plant Journal 63, 6, 901-913.

Label: Physiol Keywords: endoplasmic reticulum integrity Golgi apparatus protein traffic Abstract: Summary A central question in cell biology is how the identity of organelles is established and maintained. Here, we report on GOLD36, an EMS mutant identified through a screen for partial displacement of the Golgi marker, ST-GFP, to other organelles. GOLD36 showed partial distribution of ST-GFP into a modified endoplasmic reticulum (ER) network, which formed bulges and large skein-like structures entangling Golgi stacks. GOLD36 showed defects in ER protein export as evidenced by our observations that, besides the partial retention of Golgi markers in the ER, the trafficking of a soluble bulk-flow marker to the cell surface was also compromised. Using a combination of classical mapping and next-generation DNA sequencing approaches, we linked the mutant phenotype to a missense mutation of a proline residue in position 80 to a leucine residue in a small endomembrane protein encoded by the gold36 locus (At1g54030). Subcellular localization analyses indicated that GOLD36 is a vacuolar protein and that its mutated form is retained in the ER. Interestingly also, a gold36 knock-out mutant mirrored the GOLD36 subcellular phenotype. These data indicate that GOLD36 is a protein destined to post-ER compartments and suggest that its export from the ER is a requirement to ensure steady-state maintenance of the organelle’s organization and functional activity in relation to other secretory compartments. We speculate that GOLD36 may be a factor that is necessary for ER integrity because of its ability to limit deleterious effects of other secretory proteins on the ER. Notes: TY - JOUR URL: http://dx.doi.org/10.1111/j.1365-313X.2010.04296.x Author Address: 1Michigan State University-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA 2Department of Botany, Graduate School of Science, Emphasis Kyoto University, Sakyo-ku, Emphasis Kyoto 606–8502, Japan 3School of Life Sciences, Oxford Brookes University, Gipsy Lane, Oxford OX3 0BP, UKingdom XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Author: Marvier M Year: 2008 Title: ¤ Meta-analysis of the effects of Bt crops on non-target organisms Journal: 10th ISBGMO - 10th International Symposium on the Biosafety of Genetically Modified Organisms Biosafety research : Past Achievements and Future Challenge - Museum of New Zealand Te Papa Tongarewa Cable St., Wellington, New Zealand, Sunday 16 November - Friday 21 November 2008 http://www.isbr.info/sites/default/files/symposia/10th_symposium-2008.pdf Label: InRe ImpactBiol Abstract: With at least two decades of fi eld experiments and over a decade of commercial experience, a great deal is now known about the risks - or lack of risks - for genetically-modifi ed Bt crops. Unfortunately, risk analyses generally neglect the wealth of accumulated data, although they may call upon ―experience‖ in an anecdotal manner. Moreover, individual risk assessment studies typically are poorly replicated and thus have low statistical power. The weak power of these studies means that a fi nding of no signifi cant effect is not very convincing. However, by combining multiple studies that address a common research question via metaanalysis, a more reassuring picture can emerge.

To facilitate such meta-analyses, we assembled a global database summarizing experimental assessments of non-target effects of Bt crops. This was accomplished by drawing on the risk assessment studies that US Environmental Protection Agency (EPA) has received, as well as studies published in the peer-reviewed literature. The database includes information from both laboratory and fi eld studies of Bt cotton, eggplant,