Références sur les Plantes

Author: Kambakam Sekhar, Bhyri Priyanka, Reddy VD, Rao KV, Year: 2010 Title: * Isolation and characterization of a pigeonpea cyclophilin (CcCYP) gene, and its over-expression in Arabidopsis confers multiple abiotic stress tolerance.

Journal: Plant, Cell and Environment 33, 8.

Accession Number: CABI:20103246868 Label: ReEn Secheresse Salin Keywords: amino acid sequences; biomass; characterization; chlorophyll; complementary DNA; DNA libraries; drought; effects; environmental factors; fluorescence; gene expression; genes; genetic transformation; growth; isomerases; localization; nuclei; pigeon peas; plant development; roots; salinity; salt; salt tolerance; shoots; stress; stress conditions; stress response; survival; temperature; transgenic plants; transgenics; water stress; Capparales; cDNA; cell nuclei; genetically engineered plants; genetically modified plants; GMOs; protein sequences Abstract: A full-length cDNA clone of pigeonpea (Cajanus cajan L.) encoding cyclophilin (CcCYP) has been isolated from the cDNA library of plants subjected to drought stress. Amino acid sequence of CcCYP disclosed similarity with that of single-domain cytosolic cyclophilins of various organisms. Expression profile of CcCYP in pigeonpea plants is strongly induced by different abiotic stresses, indicating its stress-responsive nature.

Compared to the control plants, the transgenic Arabidopsis lines expressing CcCYP exhibited high-level tolerance against major abiotic stresses, viz., drought, salinity and extreme temperatures as evidenced by increased plant survival, biomass, chlorophyll content and profuse root growth. The CcCYP transgenics, compared to the controls, revealed enhanced peptidyl-propyl cis-trans isomerase (PPIase) activity under stressed conditions, owing to transcriptional activation of stress-related genes besides intrinsic chaperonic activity of the cyclophilin. The transgenic plants subjected to salt stress exhibited higher Na+ ion accumulation in roots as compared to shoots, while a reverse trend was observed in the salt-stressed control plants, implicating the involvement of CcCYP in the maintenance of ion homeostasis. Expression pattern of CcCYP:GFP fusion protein confirmed the localization of CcCYP predominantly in the nucleus as revealed by intense green fluorescence. The overall results amply demonstrate the implicit role of CcCYP in conferring multiple abiotic stress tolerance at whole-plant level.

URL: <Go to ISI>://20103246868 Author Address: Centre for Plant Molecular Biology, Osmania University, Hyderabad 500 007, A.P., India.

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Author: Kaur P, Samuel DVK, Bansal KC, Year: 2010 Title: * Fruit-specific Over-expression of LeEXP1 Gene in Tomato Alters Fruit Texture.

Journal: Journal of Plant Biochemistry and Biotechnology Volume: 19 Issue: 2 Pages: 177-183 Published: JUL 2010 .

Accession Number: WOS:000281080200006 Label: Composition QualitĂŠ Physiol Keywords: expansin, fruit softening, fruit-specific promoter, transgenic tomato.

Abstract: Expansins are cellular proteins with diverse physiological functions. Expression of fruit-specific expansin gene in tomato is associated with fruit softening - a desirable trait from the processing point of view.

In the present study, an expansin gene LeEXP1 was introduced via Agrobacterium tumefaciens in sense orientation under the control of a fruit-specific promoter LeACS4 with nptII gene as selection marker in Indian tomato cv Pusa Uphar. PCR detection and Southern blot analysis confirmed the integration of the transgene in the transformed tomato plants: RT-PCR and northern blot analysis using total RNA isolated from leaves and fruits confirmed over-expression of the LeEXP1 gene in transgenic fruits as compared to the wild type plants.

Apart from the visual change in increased red colouration of fruits at different stages of ripening, overexpression of the LeEXP1 gene resulted in enhanced fruit softening, as determined by force required to rupture the fruit pericarp, in the transgenic fruits from breaker stage onwards as compared to the nontransformed wild type fruits. The results thus suggest an improvement in texture of the LeEXP1 overexpressing fruits, which might be useful for tomato processing industry.

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