Références sur les Plantes

Accession Number: CABI:20103267246 Label: InRe Detection Devenir Keywords: detection; feeds; maize; polymerase chain reaction; transgenic plants; corn; feeding stuffs; genetically engineered plants; genetically modified plants; GMOs; PCR Abstract: The present study which dealt mainly with processed feeds and some maize samples sold commercially in Malaysia evaluated the implementation of a real-time PCR cycling system for singleplex screening of eight target sequences (lectin, hmg, adh1, p35S, NK603, GA21, MON810 and MON863) and quantification of four genetically modified (GM) maize events (NK603, GA21, MON810 and MON863). The effects of using proprietary glass magnetic particles to bind DNA to their surface were also investigated in terms of DNA quantity, purity, integrity, quality and its overall effect on DNA amplification. GM material was present in 26.2% feeds and 65% maize samples. All GM samples contained MON810 followed by NK603 (47.5%), GA21 (25%) and MON863 (2.5%). Single-event and multiple-events were identified in the GM samples with 50% of the GM samples containing multiple-events. The present study which represents a fast and reliable methodology would provide an overview of the presence and levels of GMOs in feeds and maize in Malaysia.

Notes: Cited Reference Count: 25 ref.

URL: <Go to ISI>://20103267246 Author Address: Department of Chemistry, Ministry of Science, Technology and Innovation Malaysia, Jalan Sultan, 46661 Petaling Jaya, Selangor, Malaysia.

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX Author: Javelle Marie, Vanessa Vernoud, Nathalie Depège-Fargeix, Christine Arnould, Delphine Oursel, Frédéric Domergue, Xavier Sarda, Peter M. Rogowsky, Year: 2010 Title: * Overexpression of the Epidermis-Specific Homeodomain-Leucine Zipper IV Transcription Factor OUTER CELL LAYER1 in Maize Identifies Target Genes Involved in Lipid Metabolism and Cuticle Biosynthesis.

Journal: Plant Physiology 154:273-286 (2010).

Accession Number: MEDLINE:20605912 Label: Physiol Abstract: Transcription factors of the homeodomain-leucine zipper IV (HD-ZIP IV) family play crucial roles in epidermis-related processes. To gain further insight into the molecular function of OUTER CELL LAYER1 (OCL1), 14 target genes up- or down-regulated in transgenic maize (Zea mays) plants overexpressing OCL1 were identified. The 14 genes all showed partial coexpression with OCL1 in maize organs, and several of them shared preferential expression in the epidermis with OCL1. They encoded proteins involved in lipid metabolism, defense, envelope-related functions, or cuticle biosynthesis and include ZmWBC11a (for white brown complex 11a), an ortholog of AtWBC11 involved in the transport of wax and cutin molecules. In support of the annotations, OCL1-overexpressing plants showed quantitative and qualitative changes of cuticular wax compounds in comparison with wild-type plants. An increase in C24 to C28 alcohols was correlated with the transcriptional up-regulation of ZmFAR1, coding for a fatty acyl-coenzyme A reductase. Transcriptional activation of ZmWBC11a by OCL1 was likely direct, since transactivation in transiently transformed maize kernels was abolished by a deletion of the activation domain in OCL1 or mutations in the L1 box, a cis-element bound by HD-ZIP IV transcription factors. Our data demonstrate that, in addition to AP2/EREBP and MYBtype transcription factors, members of the HD-ZIP IV family contribute to the transcriptional regulation of genes involved in cuticle biosynthesis.

URL: http://www.plantphysiol.org/cgi/content/abstract/154/1/273 Author Address: Université de Lyon, Ecole Normale Supérieure de Lyon, Université Lyon 1, Institut Fédératif de Recherche 128 BioSciences Lyon Gerland, Unité Reproduction et Développement des Plantes, F–69364 Lyon, France INRA, UMR879 Reproduction et Développement des Plantes, F–69364 Lyon, France CNRS, UMR5667 Reproduction et Développement des Plantes, F–69364 Lyon, France Centre de Microscopie INRA/Université de Bourgogne, INRA, Centre de Microbiologie du Sol et de l'Environnement, F–21065 Dijon, France Laboratoire de Biogenèse Membranaire, Université Bordeaux II, CNRS-UMR5200, F–33076 Bordeaux, France Biogemma, Laboratoire de Biologie Cellulaire et Moléculaire, F–63028 Clermont-Ferrand, France