NEW Fluorescence Imaging Interference Filters
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Introducing NEW multiband filters for fluorescence detection Available individually or as sets. Each set has 3 multi bandpass filters (excitation, emission, dichroic), and the “Pinkel” versions of these three new sets contain separate narrow band excitation filters for selected excitation of each fluorophore. Multi band pass filters are widely used in fluorescence imaging applications as a means of rapidly identifying positively labeled multi color samples. The filters are optimized to provide high transmission, steep edges and deep blocking for balance in contrast, brightness, and color representation. All filters are manufactured using Omega Optical’s proprietary MEGALux © optical coating technology. Size
Excitation and Emission Filters:
12.5, 18, and 25 mm round
Custom sizes up to 55 mm available. Comparable pricing to catalog list prices. Call for details.
Dual Band Filters Fluorophores
FITC/ TRITC or eGFP/ DsRed2
FITC/Texas Red® or eGFP/mCherry
FITC/ Cy5®
FITC/TRITC or eGFP/DsRed2
FITC/Texas Red® or eGFP/mCherry
FITC/ Cy5®
Filter Set #
XF452
XF453
XF454
XF452-1
XF453-1
XF454-1
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Dichroic Beamsplitters: 18x26, 25.7x36 mm rectangular, and 25 mm round
Notes
Components Type
Part #
Description
Exciter
XF1450
490-550DBEX
Dichroic
XF2443
490-550DBDR
Emitter
XF3456
520-580DBEM
Exciter
XF1451
484-575DBEX
Dichroic
XF2044
490-575DBDR
Emitter
XF3457
525-637DBEM
Due to their wavelength separation, FITC and Cy5 make a popular choice for dual labeling in a single sample as spectral bleedthrough is virtually non-existent. This set is ideal for any green and far red emitting fluors. Other compatible dyes are, Alexa Fluor®488, Hylite 488, Oregon Green, Cy2, and Alexa Fluor®647, Hylite 647.
Exciter
XF1409
475-625DBEX
Dichroic
XF2401
475-625DBDR
Emitter
XF3404
535-710DBEM
Pinkel – a set contains 2 bandpass excitation filters, single multiband dichroic beamsplitter and single multiband emission filter.
Exciter #1
XF1404
480QV20
Exciter #2
XF1405
555QV25
Dichroic
XF2443
490-550DBDR
Emitter
XF3456
520-580DBEM
Exciter #1
XF1406
490QV20
Exciter #2
XF1407
575QV30
Dichroic
XF2044
490-575DBDR
Emitter
XF3457
525-637DBEM
Exciter #1
XF1405
480QV20
Exciter #2
XF1410
630QV40
Dichroic
XF2401
475-625DBDR
Emitter
XF3404
535-710DBEM
High throughput filter set for green and orange emitting fluorophores such as FITC and TRITC. This set offers excellent contrast and can also be used with Alexa Fluor® 488, Cy2, and GFP-like fluorescent proteins, as well as Alexa Fluor®568 and tdTomato. XF453 is optimized for use with the fluorescent proteins eGFP and mCherry. This high contrast set utilizes the 577nm Mercury peak for efficient excitation of red emitting fluorophores and is also compatible with many other common fluors such as FITC and Texas Red®.
Pinkel – a set contains 2 bandpass excitation filters, single multiband dichroic beamsplitter and single multiband emission filter.
Pinkel – a set contains 2 bandpass excitation filters, single multiband dichroic beamsplitter and single multiband emission filter.
XF467 Triple stained images of mouse brain (page 1) and Muntjac deer cells (page 2). Courtesy of Mike Davidson at the National High Magnetic Lab, Florida State University.
Size
Excitation and Emission Filters:
12.5, 18, and 25 mm round
Dichroic Beamsplitters: 18x26, 25.7x36 mm rectangular, and 25 mm round
Custom sizes up to 55 mm available. Comparable pricing to catalog list prices. Call for details.
Triple Band Filters Fluorophores
Filter Set #
DAPI/FITC/Texas Red® or BFP/eGFP/ mCherry
XF467
DAPI/FITC/Texas Red® or BFP/eGFP/ mCherry
XF467-1
Notes
Components Type
Part #
Description
This filter set is optimized for use with common blue, green, red emitting fluors such as DAPI/ FITC/Texas Red® or proteins BFP/eGFP/mCherry. The set can be used with visual detection, a CCD camera or color film for image capture.
Exciter
XF1458
390-486-577TBEX
Dichroic
XF2045
400-485-580TBDR
Emitter
XF3458
457-528-600TBEM
Pinkel
Exciter #1 Exciter #2 Exciter #3 Dichroic Emitter
XF1408 XF1406 XF1407 XF2045 XF3458
405QV20 490QV20 575QV30 400-485-580tbdr 457-528-600tbem
pinkel FILTER sets Obtaining a high quality image using a black and white CCD camera requires a different approach. Pinkel filter sets selectively excite one fluorophore in a multi labeled sample through the use of fluor specific narrow band excitation filters housed in a filter wheel or slider. These filters are used with a multi band dichroic mirror and emission filter which remain in the filter holder turret. This design reduces the spectral bleed-through of the traditional multiband filter set, and minimizes sample movement by isolating the wheel from the microscope stage. XF467-1 Triple Band Set for DAPI/FITC/ Texas Red® 100 90 80
60 50
XF1408 405QV20 XF1404 490QV20 XF1407 575QV30 XF2045 400-485-580TBDR XF3458 457-528-600TBEM
40 30 20 10 0 350
For more information on all stock filters 400
450
500
550
600
650
700
along with an expanded list of compatible dyes,
nm
interactive tool Curvomatic at www.omegafilters.com
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Transmission %
70