LifeasibleProvidesPlantGeneOverexpressionServiceforResearchUse
Lifeasible,withdecadesofexperienceinplantgeneticengineering,addressesthegeneticmodification ofplantsthroughmultiplepopulargeneticengineeringtechnologiesNowthecompanyoffersplant geneoverexpressionservicetoaccommodateresearchneeds
Geneoverexpressionreferstocloningatargetgeneintoavectorcarryingelementssuchasastrong promoterandaresistanceselectionmarker,andthenintroducingitintotheplant,sothatthehostcell willobtainahigheramountoftargetmRNAtranscriptionlevelandproteinexpressionlevel,sothatthe functionofthegenecanbestudiedthroughanalysissuchasphenotypeItissuitableforsituations wheregenefunctionisredundantorlethalaftergeneknockout
Geneoverexpressionhasbeenusedasanalternativeoradditionalapproachtoloss-of-function approachesaswellastoconfernewfunctionsonplantsinordertoelucidateplantgenefunction.
Lifeasible,withcutting-edgefacilitiesandabundantexperience,isproudtoofferflexibleand dependableone-stopgeneoverexpressionservicestohelpacceleratedifferentresearchneeds, including:
Vectorconstructionwithdifferentpromoters
Forvariousneeds,avarietyofpromotersareavailableatLifeasible,includingthoseforconstitutive (suchasp35s,pUbi,pActin1,andpNos),tissue-specific(suchaspRBCSforleaves),inducible(suchas pCABforlight,pRD29Aforstress,andpERforestradiol)expression,andtissue-specific(suchas pAP3forflowers,pGSSPforgreentissue)
Vectorconstructionwithdiversefusionprotein
Functioninvestigationoftargetgenes,suchassubcellularlocalization,proteininteraction,ordirectly regulatedgenedetection,canbecarriedoutusingconstructswithfusedproteinGFP,His,GST,MYC, orGR.
Vectorassembly
Lifeasiblealsocreatestechnologiesforvectorassemblywithmultipleopenreadfragments(ORFs)for theoverexpressionofnumerousgenes.
Planttransformation
Themajorityoftransformationsystems,includingthoseinmonocotspeciesanddicotmodelplantslike rice,wheat,barley,soybean,andArabidopsis,arecoveredatLifeasible.
Traitmeasurementandgenefunctionanalysisoftransgenicplants
Lifeasiblehasestablishedaone-stopserviceplatformforplants,pleasevisit https://wwwlifeasiblecom/custom-solutions/plant/genetically-modified-plants/gene-overexpression-inplants/toknowmore