LC and LC/MS Columns
HPLC Column
Stationary Phase
Type of Surface
Pore Size
Column Dimensions
Particle Size
Inner Diameter
Length
Chemical Properties
Physical Properties
Chemical Lifetime/Selectivity Retention Factor
Efficiency Sensitivity Speed
Column Choice Relative to Application Objective Application Very high sensitivity, LC/MS, peptides and proteins Very high sensitivity, limited sample, LC/MS, peptides and proteins High sensitivity, limited sample, LC/MS Save solvent; special low-volume instrumentation is available Special detectors, e.g., mass spec High sensitivity, limited sample Save solvent; standard HPLC equipment available, LC/MS Standard separations Small-scale (mg) preparative separations Large-scale preparative separations (100 mg-gram) Large-scale preparative separations (up to 100 mg-gram)
Objective Column Diameter (mm) 0.1, 0.075 0.3, 0.5 1.0 2.1 2.1 2.1 3.0 4.6 9.4 21.2 30, 50
Consult the Column Hardware section for guard column configurations
LC AND LC/MS COLUMNS
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