Columns for Special Applications
Chiral HPLC Columns Ultron ES Chiral Columns • Direct racemic separations without derivatization • Use Ultron ES-OVM as the USP L57 choice and to separate enantiomers of acidic and basic pharmaceuticals, such as hexobarbital, ibuprofen, and profenamine
• Ultron ES-Pepsin Chiral columns are best suited to separate basic compounds that are difficult to separate with other chiral columns
• ES-OVM and ES-Pepsin columns contain 120Å, 5 μm silica particles bonded with an ovomucoid protein and pepsin protein, respectively
• Both types of chiral columns are usable with reversed-phase mobile phases such as acetonitrile or ethanol and phosphate buffer Ultron ES Chiral columns are immobilized protein columns that feature numerous chiral recognition sites for enantiomeric separations of dozens of chiral compounds. They are engineered with two complementary protein-based chiral stationary phases, making them an excellent choice for the HPLC separation of enantiomers without derivatization – including a growing number of drug substances of interest.
Separation of Enantiomers of Fluoxetine (Prozac) Column:
Ultron ES-OVM Chiral 702111651 4.6 x 150 mm, 5 μm
Mobile Phase:
25:75 (v/v) EtOH/20 mM KH2PO4, pH 5.5 (adjusted with NaOH) Ambient UV (225 nm) Mixture Fluoxetine (Prozac) entantiomers
Temperature: Detector: Sample:
0
Courtesy of D. S. Risley and V. S. Sharp of Lilly Research Laboratories, Eli Lilly and Co.
5
10 15 Retention Time (min)
20
25
30 LCUC001
LC AND LC/MS COLUMNS
913