Columns for Analytical HPLC
Pursuit XRs Silica is another choice for normal-phase chromatography. For more information, see page 862–863.
ZORBAX CN • Cyanopropyldimethylsilane monolayer bonded to ZORBAX SIL • Equilibrates more rapidly than ZORBAX SIL, and used for many of the same normal-phase applications
• Less prone to fouling and less water sensitive than silica ZORBAX NH2 • Amino-propyl silane phase bonded to ZORBAX SIL • Used for normal-phase and weak anion-exchange, and reversed-phase HPLC of polar compounds • Vitamins A and D are separated in the normal-phase mode • Carbohydrates and sugars are separated in the reversed-phase mode
Column Specifications Bonded Phase ZORBAX Rx-SIL ZORBAX Eclipse XDB-CN ZORBAX SIL ZORBAX CN ZORBAX NH2
Pore Size 80Å 80Å
Surface Area pH Range 0-8.0 180 m2/g 2.0-8.0 180 m2/g 300 m2/g 300 m2/g 300 m2/g
70Å 70Å 70Å
Endcapped Carbon Load No Yes 4.3%
0-8.0 2.0-7.0 2.0-7.0
No Yes Yes
7% 4%
High Resolution Normal-Phase Separation of Octylphenoxy Ethanol Surfactant on ZORBAX CN Column:
ZORBAX CN 880952-705 4.6 x 250 mm, 5 μm
Mobile Phase:
Primary: Heptane Secondary: 2-Methoxyethanol/Isopropanol (50/50) 2 mL/min 2-20% Secondary in 10 min., Linear Hold at 20% 50°C 278 nm Octylphenoxy (polyethylene oxy) Ethanol Surfactant (n= 10)
Flow Rate: Gradient: Temperature: Detector: Sample:
0
5
10
15 Time (min)
20 LCNP001
874
LC AND LC/MS
www.agilent.com/chem/lccolumns