Columns for Analytical HPLC
Quick Guide to Agilent Reversed-Phase Bonded Phases Modern RP-HPLC Columns Extend-C18
Recommended Uses and Applications Separating basic compounds above their pKa in free base form; separation of basic, acidic, neutral compounds at high pH; up to pH 11.5 Uses ammonium hydroxide as mobile phase additive with LC/MS with small molecules or peptides Separating at high, mid-range and low pH for selectivity changes Rapid separations using 3.5 Îźm columns
Page No. 850
ZORBAX Rx
General separation of basic, acidic and neutral compounds at low pH with different selectivity than SB columns Rx-C8 is the same as SB-C8
854
Pursuit
Good separations of a wide range of analytes Diphenyl and Pentafluorophenyl bonded phases for unique selectivity 200Ă… pore size for separations of larger molecules
856
Pursuit XRs
High carbon load for excellent retention and resolution Basic, acidic, and neutral compounds Unique diphenyl bonded phase for separations based on pi-pi selectivity
862
Polaris A
Good for polar acids, polar bases and non-polar compounds High aqueous compatibility
865
Polaris Ether
Additional selectivity for H-bond donors High aqueous compatibility No "phase collapse"
865
Recommended Uses and Applications
Page No. 870
Original ZORBAX Columns ZORBAX
General separation of basic, acidic, neutral compounds at low pH with different selectivity than SB columns; higher number of active silanols than SB "Mixed mode" separation at more neutral pH values
ZORBAX ODS Classic General separation of basic, acidic, neutral compounds at mid-range to low pH with different selectivity than SB or XDB columns (non-endcapped)
870
LC AND LC/MS COLUMNS
821