Flow Cytometry Core

HOUSTON METHODIST RESEARCH INSTITUTE

FLOW CYTOMETRY CORE Located at the Houston Methodist Research Institute in Houston, TX, the Flow Cytometry Core is staffed by experienced research professionals and laboratory technicians who provide training, instrumentation, technical expertise and software for flow cytometric analysis and cell sorting.

We offer comprehensive analyzer training, assistance in the design of experimental protocols, and ongoing consultation and support.

We look forward to serving you with customized projects that will enhance your activities in a reliable and timely manner.

Flow Cytometry Core Director: David L. Haviland, Ph.D., C. Cy. Email: dlhaviland@houstonmethodist.org 713.441.9233

houstonmethodist.org/flowcytometry

Houston Methodist Research Institute 6670 Bertner Avenue Houston, TX 77030

houstonmethodist.org/research HMRI Communications & External Relations | RICORE-004 | RH | DH | 500 | 02.2013

FLOW CYTOMETRY EQUIPMENT These instruments allow scientist to make very concise measurements at the cellular level, evaluate a large number of samples in a short time frame, and gather information on very rare populations of cells.

Flow Cytometry Core

• B D FACS LSRII - A five laser instrument with 355, 405, 488, 561, and 630 nm excitation sources, capable of 14 color analysis

FACILITY

FORTESSA

The Flow Cytometry Core uses fluorescent probes, usually labeled antibodies, to identify cells. Information about the reative size and granularity of a cell is recorded and the result is a visual presentation describing an individual or group of cellular events. If needed, the cells or particles can be separated by sorting and further analyzed.

• NIR

BD FACS AriaII - A six laser instrument with 355, 405, 488, 550, 635, and 785 nm excitation sources, capable of 18 color analysis. This instrument is equipped with an Forward Scatter PMT in addition to the FSC photodiode. This instrument is capable of high speed sorting, sample depending. Temperature controlled sample and collection chambers with four-way and single cell deposition are available.

• S ORP BD FACS AriaII – A four laser instrument with 405, 488, 550, and 635 nm excitation sources capable of 13 color detection. This instrument is also capable of high speed sorting, sample depending. Temperature controlled sample and collection chambers with four-way and single cell deposition are available.

Our Specialized Services

• A Millipore Guava 8-HT – A two laser instrument with 488 and 635 nm excitation sources capable of six color detection. The 8-HT provides rapid 96-well handling capability for screening assays.

Flow Cytometry core staff will advise on:

LSRII

The Houston Methodist Research Institute Flow Cytometry Core provides training, instrumentation, technical expertise and software for flow cytometric analysis and cell sorting.

• B D FACS Fortessa - A four laser instrument with 405, 488, 561, and 630 nm excitation sources, capable of 13 color analysis, and equipped with a Forward Scatter PMT in addition to the FSC photodiode

• Experimental design

• Sample preparation

• Controls

• Excitation and detection

• Data Interpretation

• Manuscript preparation