EpiVax Methods
24 HOURS
Test peptide (predicted by EpiMatrix) Biotinylated high-affinity control peptide HLA class II monomer
Y Y Y Y YY Y Y Europium-labeled Streptavidin only targets biotinylated peptides
Confidential
Unbound peptide removed in wash step
Y Y YY YY Y Y High-absorbance ELISA capture plate
Fluorescence counts are converted into percent inhibition of biotinylated standard
PLATE READER (EU+)
Each test peptide is assayed at multiple concentrations If dose-dependence curves are observed, IC50 values are calculated using SigmaPlot software The lower the IC50 the higher the binding affinity of the test peptide