CENTRAL ASIAN JOURNAL OF MEDICAL AND NATURAL SCIENCES Volume: 03 Issue: 05 | Sep-Oct 2022 ISSN: 2660-4159 http://cajmns.centralasianstudies.org
Studying the Relationship of Local Immune Status and the Course of Burn Disease 1. Abdullaev Xasan Davlatovich 2. Shodiyev Dilmurod Orifovich 3. Amirov Sirojiddin Baxtiyorovich
Received 28th Aug 2022, Accepted 29th Sep 2022, Online 31st Oct 2022
1
Samarkand State Medical University Department of Skin and Venereal Diseases Samarkand, Uzbekistan 2, 3
Student of Samarkand State Medical University, Samarkand, Uzbekistan
Abstract: this article summarizes a study of 14 patients of the Russian Burn Center; an immunohistochemical study of scar tissue biopsy specimens was carried out in order to study the number and nature of the distribution of immunocompetent cells in the epidermis and dermis. To assess the parameters of local immunity in biopsy specimens of scar tissue, an analysis was made of the topography and content of T-lymphocytes (CD3positive cells), as well as CD4 and CD8 co-receptors, the distribution and quantitative characteristics of Blymphocytes in pathologically altered skin, CD 25positive cells, topography and content of intraepidermal macrophages (Langerhans cells) in the skin. The task of our work included the study of the relationship between the local immune status and the course of burn disease. Key words: burn disease, immune status.
Introduction: "Burn disease" redirects here. On this topic, you need to create a separate article. This term has other meanings, see Burn (meanings). Burn - damage to body tissues caused by the action of high temperature, the action of certain chemicals (alkalis, acids, salts of heavy metals, and others). There are 4 degrees of burns: 1. skin redness, 2. blistering, 3. necrosis of the entire thickness of the skin, 4. charring of tissues. The severity of the burn is determined by the size of the area and the depth of tissue damage. The larger the area and the deeper the tissue damage, the more severe the course of the burn injury. First aid for burns is to stop the impact of external factors and treat the wound. Special forms: radiation burn - a burn caused by ionizing radiation and electric burn - a burn during electrical injury. Burns caused by ultraviolet radiation (solar, welding, artificial light sources) and intense visible radiation are called photodermatitis and photokeratitis. Burns caused by high-intensity
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CAJMNS
Volume: 03 Issue: 05 | Sep-Oct 2022
microwave or light radiation (eg, laser) are thermal[1][2][3][4][5]. Intense visible spectrum radiation, including laser radiation, can also cause retinal burns. The branch of medicine that deals with the treatment of burns and related pathological conditions is called combustiology. Burns are one of the most common traumatic injuries in the world. So, in Russia in 1997, 507.6 thousand people were registered who received various burns [7]. In terms of deaths, burns are second only to injuries sustained in car accidents. The treatment of burns is a difficult and multifaceted event: thermal damage is one of the most dangerous, they lead to the destruction of complex proteins - the basis of cells and tissues. Purpose: to study the relationship between local immune status and the course of burn disease. Materials and methods: 14 patients of the Russian Burn Center underwent an immunohistochemical study of scar tissue biopsy specimens in order to study the number and nature of the distribution of immunocompetent cells in the epidermis and dermis. To assess the parameters of local immunity in biopsy specimens of scar tissue, an analysis was made of the topography and content of Tlymphocytes (CD3-positive cells), as well as CD4 and CD8 co-receptors, the distribution and quantitative characteristics of B-lymphocytes in pathologically altered skin, CD 25-positive cells, topography and content of intraepidermal macrophages (Langerhans cells) in the skin. We used test kits from MedBioSpektr, Moscow, and test systems from DAKO®. Determination of the content of Tlymphocytes and subpopulations of T-cells (T-helpers and suppressor-cytotoxic T-lymphocytes) was carried out by indirect immunofluorescence using MCA IKO-90, IKO-86 and IKO-31 (for CD3, CD4 and CD8 antigens, respectively). ). The number of T-lymphocytes and subpopulations of T-cells in the epidermis was counted per 1000 epidermocytes. The results were expressed in ppm. The number of B-lymphocytes in the skin was studied per 1000 epidermocytes by indirect immunofluorescence using MCA ICO-91 to the CD22 marker. The study of IL-2p expression by immunocompetent cells in the skin was carried out by immunofluorescence using MCA ICO-105 to CD25 antigen. The study of the topography and quantitative characteristics of resident macrophages (Langerhans cells, CL) in the skin was carried out by enzyme immunoassay using streptavidin-biotin technology (sets with the Universal DAKO LSAB 2 kit, Peroxidase) and MCA to S100 protein. Results: in the study of biopsy specimens of scars, the predominant location of T-lymphocytes and their helper and suppressor subpopulations in the epidermis was revealed. However, in 37.5% of patients, accumulations of T-lymphocytes were found in the dermis around capillary venules; in 3 patients, exceptional localization of T-lymphocytes in the dermis was observed. The level of T-lymphocytes in the epidermis revealed a sharp, statistically significant decrease compared to the control group (6.47 ± 2.95% and 19.6 ± 0.6%, respectively, p<0.001). The study of the helper and suppressor subpopulations also showed their decrease, in the case of CD8, approaching statistically significant. In normal skin, CD22-positive cells in the epidermis were absent in most of the biopsy specimens, being located in 3-4 rows around post-capillary venules in the dermis. Only four people (14.3%) in the control group had weak immunofluorescence of B-lymphocytes in the epidermis, which were localized in the basal layer and suprabasally.
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In scar biopsy specimens, the presence of B-lymphocytes in the epidermis could be determined in 6 patients (43%). Their average level (8.88 4.45%) exceeded the indicators of the control group (up to 6.4%). In the dermis of 4 patients, a clear glow was observed around the capillary venules. The study of the correlation coefficient between B-lymphocytes and subpopulations of T-lymphocytes did not reveal pronounced differences compared with the control group. In the skin of healthy individuals (control group), cells expressing IL-2p were localized in the basal layer or suprabasally. Rare accumulations of CD25-positive immunocompetent cells were found in the dermis, most of which were located in 2-3 rows around the postcapillary venules of the superficial vascular plexus and skin appendages. In the epidermis of cicatricial biopsy CD25+ lymphocytes were found only in one patient (2.8% with control values up to 14 ‰). In the dermis of 6 patients, a slight intensity of luminescence around the capillary venules was observed. Conclusions: Immunohistochemical studies of biopsy specimens of scar tissue from post-burn patients revealed inhibition of the SALT T-cell link. Based on the study of IRI and the high correlation coefficient between CD3+ and CD8+ lymphocytes, it can be concluded that the predominant activation of the suppressor link in the formation of scar tissues. The study of Langerhans cells showed their active interaction with both T-cell and humoral immunity in the skin of post-burn patients. Literature: 1. Iskandarovna K. M., Alamovich K. A., Rabbimovich N. A. Treatment of Urethrogenic Prostatitis Associated with Chlamydia Infection //TA'LIM VA RIVOJLANISH TAHLILI ONLAYN ILMIY JURNALI. – 2021. – Т. 1. – №. 5. – С. 44-46. 2. Iskandarovna K. M., Buribaevna I. S., Azamovna A. N. Immunoassay Forms of Syphilis //TA'LIM VA RIVOJLANISH TAHLILI ONLAYN ILMIY JURNALI. – 2021. – Т. 1. – №. 5. – С. 47-49. 3. Iskandarovna K. M. SIFILISNING IMMUNOASSAY SHAKLLARI //BARQARORLIK VA YETAKCHI TADQIQOTLAR ONLAYN ILMIY JURNALI. – 2022. – С. 534-536. 4. Ахмедова М., Расулова Н., Абдуллаев Х. Изучение парциальных функций почек у детей раннего возраста с нефропатией обменного генеза //Журнал проблемы биологии и медицины. – 2016. – №. 2 (87). – С. 37-40. 5. Нуруллаева А. А., Рахматова А. Х., Абдуллаев Х. Д. ЗНАЧЕНИЕ МИКРОБНОГО ОБСЕМЕНЕНИЯ КОЖИ ПРИ НЕКОТОРЫХ ЗУДЯЩИХ ДЕРМАТОЗАХ //Молодежь и медицинская наука в XXI веке. – 2019. – С. 125-125. 6. Abdullaev X. D. et al. EVALUATION OF THE EFFECTIVENESS OF THE DRUG GEPON IN THE TREATMENT OF GENITAL CANDYLOMAS //Web of Scientist: International Scientific Research Journal. – 2021. – Т. 1. – №. 02. – С. 16-19. 7. Davlatovich A. X. VAGINAL TRIXOMONADLAR SHTAMMASINI TRIXOPOLGA VA XIMOTRIPSIN BILAN BILAN SEZGICHLIGINI ANIQLASH //BARQARORLIK VA YETAKCHI TADQIQOTLAR ONLAYN ILMIY JURNALI. – 2022. – С. 645-647. 8. Xolmurodovich D. J., Orifovich R. S., Davlatovich A. X. FEATURES OF THE MICROELEMENT STATUS FOR THE DEVELOPMENT OF ATOPIC DERMATITIS IN CHILDREN //BARQARORLIK VA YETAKCHI TADQIQOTLAR ONLAYN ILMIY JURNALI. – 2022. – С. 447-450. 9. Davlatovich A. X. DETERMINATION OF GENE ACTIVITY IN VITILIGO PATIENTS //BARQARORLIK VA YETAKCHI TADQIQOTLAR ONLAYN ILMIY JURNALI. – 2022. – С.
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451-454. 10. Davlatovich A. X. et al. EVALUATION OF THE GENERAL SOMATIC STATUS OF PATIENTS WITH VITILIGO BASED ON THE DETERMINATION OF THE CONTENT OF NATURAL ANTIBODIES TO VARIOUS ORGANIS AND TISSUES OF THE BODY //BARQARORLIK VA YETAKCHI TADQIQOTLAR ONLAYN ILMIY JURNALI. – 2022. – С. 472-476. 11. Davlatovich A. X. et al. USING IMMUNOMAX AND 0.1% TACROLIMUS OINTMENT IN THE TREATMENT OF VITILIGO //BARQARORLIK VA YETAKCHI TADQIQOTLAR ONLAYN ILMIY JURNALI. – 2022. – С. 559-562. 12. Xolmurodovich D. J., Umidovich N. T., Davlatovich A. X. CLINIC COURSE OF NONSPECIFIC PNEUMONIA //BARQARORLIK VA YETAKCHI TADQIQOTLAR ONLAYN ILMIY JURNALI. – 2022. – С. 510-513. 13. Абдуллаев Х. Д. и др. ЛАЗЕР В ЛЕЧЕНИИ ВИТИЛИГО //BARQARORLIK VA YETAKCHI TADQIQOTLAR ONLAYN ILMIY JURNALI. – 2022. – С. 495-500. 14. Baxadirovich T. I. Topical Treatment of Children with Atopic Dermatitis //Texas Journal of Medical Science. – 2022. – Т. 8. – С. 132-134. 15. Baxadirovich T. I. AKNE VULGARNIKSNI DAVOLASH SAMARALIGINI BAHOLASH //Eurasian Journal of Medical and Natural Sciences. – 2022. – Т. 2. – №. 3. – С. 41-44. 16. ТИБ АбдуллаевХ.Д. Тошев С.У. 17. ДОСТИЖЕНИЯ И ПРОБЛЕМЫ ФУНДАМЕНТАЛЬНОЙ НАУКИ И КЛИНИЧЕСКОЙ МЕДИЦИНЫ 1, 571-572, 2021 18. Аликулова М. Т., Тиллакобилов И. Б. кандиФлЮ нео пРи кандидоЗе кРупныХ Складок //Forcipe. – 2020. – Т. 3. – №. S. – С. 221-222. 19. Нарзикулов Р. М. и др. Новый подход в лечении витилиго //НАУЧНЫЕ ИССЛЕДОВАНИЯ. – 2019. – С. 60. 20. Тошев С. У., Сулаймонов А. Л., Тиллакобилов И. Б. ТЕРАПИЯ ВИТИЛИГО С ПРИМЕНЕНИЕМ ПОЛИОКСИДОНИЯ В СОЧЕТАНИИ С ЛЮКОДЕРМИНОМ //Высшая школа: научные исследования. – 2019. – С. 55-59.
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