Understanding Different Pipette Tips and Their Applications Numerous Pipette Tips are widely used in medical and pharmaceutical labs for versatile applications, from developing medicines to essential vaccines. However, its development is crucial to making it able for critical applications. This is why the quality of this lab equipment plays an essential role. You need to select these instruments from a trusted supplier. For instance, choose a pipette tips supplier that is known in the market and has good reviews from buyers.
In this blog, we will look at the types of pipette tips in detail and know the applications of some of them. So let's get to it without further ado.
Non-Sterile and Sterile Tips As every single molecule and drop matters in developing medical drugs, an inaccurate proportion of the drug during pipetting might bring unpleasant outcomes. It can ruin the testing and experiments during molecular interaction, spectrophotometric and chromatographic analysis. Also, the tip material should not spread contamination in the sample. This is why sterile tips are a more reliable and efficient choice for pipetting to fetch desirable results. Although, autoclavable non-sterile tips are widely used across laboratories where contamination doesn't matter much. Also, non-sterile tips are less expensive than sterile tips.
However, it would help if you considered the below parameters for good outcomes. • Hand hygiene • Use Non-Sterile Gloves, Masks, eyewear • Ensures that it's able to autoclavable or not. While tip manufacturers verify their sterilization process by performing regular quality checks, individual labs do not test the effectiveness of the autoclaving process
These Tips are Free of RNase and DNase You need to verify if the sterile tips you opt to purchase from a manufacturer are free of RNase and DNase. These elements include the enzymatic breakdown of DNA and RNA, which are harmful. So choose to buy tips from a manufacturer that certifies their tips are free of RNase and DNase. Some of the quality tips are mentioned below.
Filter Tips Even the most minor quantities of DNA from previous samples can contaminate the results and amplify the contamination. Using filter tips when handling liquids that could hamper your pipette or the outcomes is vital. Below is a list of fluids that should always be pipetted with filter tips to prevent contamination or damage to the pipette: • Strong bases and acids • Infectious samples • Corrosive samples Application: These tips are used for training new lab staff. It will prevent contamination or damage from entering the pipette's lower end, making them a safer choice than non-sterile tips.
Long Tips The length of tips plays a significant role in avoiding risking cross-contamination. Crosscontamination can happen by putting the pipette shaft into a tube for a tip to reach the bottom. Application: These tips are used to remove the risk of contamination, making them a safe and preferred choice.
Short Tips Short Tips has a significant benefit as they provide improved ergonomics and allow you to pipette closer to the bench.
Application: These tips are majorly used in the medical domain, especially in laboratory applications where sterility is not crucial to attaining.
Wide-Bore Tips Some cellular samples may get damaged when they are forced through the narrow paths of standard tips. So you should use wide-bore trips to transfer viscous components as the wider orifice of these tips will reduce the flow resistance. Application: These tips will keep the genomic DNA (gDNA) intact, which will lessen the chance of shearing during pipetting.
Low Retention Tips These types of tips offer the most accurate results. Low retention tips are more precise and consistent than other types while saving some critical reagents. However, the cost varies too. Low retention tips cost more than other standard tips, so you should buy them only when you know if it is worth using them. Application: These tips are beneficial when you want to pipette solutions with low surface tension as compared to water.
Wrapping Up Hopefully, you now know the types of pipette tips and their applications. If you are looking for a laboratory equipment supplier Canada, contact Clinivex. They are a leading lab equipment manufacturer that supplies pipettes and other medical equipment to different countries. If you want to buy such equipment in bulk, go through their website and contact them for quality equipment.
Source: https://theclinivex.mystrikingly.com/blog/understanding-differentpipette-tips-and-their-applications
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