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Faustino A. Toba, PhD. Bachelor in Biological Science (Universidad de Venezuela) PhD. In Microbiology (Cornell University) Postdoctoral Research Scientist (Columbia University)


Introduccion Briefly, and to summarize my background, in 1994 after graduating with honors from high school I started my Biology bachelor degree at the Universidad Central de Venezuela (UCV) in 1994. During those years I did an internship in the Jardin BotĂĄnico de Caracas (Botanical Garden) where I learned basic concepts on plant identification, taxonomy and registry database. During the course of my studies I enrolled in teaching experience activities becoming a Teaching Assistant for both the Genetics and Microbiology courses for 2 full years (4 semesters in total) with an average class size of 16 students per section. In these Teaching assistantships I learned laboratory class management, coordination of lab activities and student assistance. Also, during this period I joined the PlĂĄsmidos Bacterianos (Bacterial Plasmid) lab at the Instituto de BiologĂ­a Experimental (Experimental Biology Institute) where I learned basic concepts on lab work which includes bacteria culture, sterile techniques, DNA isolation and handling, gel electrophoresis, bacterial susceptibility assays, antibiotic resistance. Was in this lab where I worked and completed my Undergrad Thesis on bacterial plasmids isolated from aquatic environments which was honored with an award the following year. I graduated with honors and first of the class in 2000 with a specialization on Cellular Biology. After graduation I was hired by the same lab as research assistance. In this position a learned lab management and project coordination. Also, I helped in the training of new students in general lab techniques. During this


year I was hired as Instructor for the Cellular Biology Lab course in the Biology department at Universidad Central de Venezuela (UCV). In 2002 I was hired for a short period at the Petroleum Biology Lab at Instituto de Estudios Avanzados (Institute of Advance Studies) where I participated in a petroleum degradation project. In 2002 I was accepted to attend the Department of Microbiology at Cornell University to pursue my PhD degree with a two year scholarship. During this period I became a Teacher Assistant for the General Microbiology class for two semesters. I joined Dr. Anthony Hay’s Environmental Microbiology and Toxicology lab as a Graduate Research Assistant. In this lab, at first, I studied the use of bioreporters for the direct detection of chemicals in the soil. In 2003, and what it will become the subject of my Thesis work, I joined a project to study E. coli biofilms. In this period I learned Molecular Biology techniques like DNA cloning, knockout mutation protocols, RNA isolation, promoter expression systems, PCR, microscopy (light and electron), tissue culture, invasion assays, image software and other research techniques related to biofilm study. During this period I help to train several undergraduate students, graduate students and technical personnel. I also help to coordinate a biofilm research group consisting of two undergraduates and a technician. As part of my experience at Cornell I also took part in several interdepartmental collaborations. I finish my Graduate studies in January 2008 and obtain a PhD in Microbiology with Biochemistry and Toxicology as minor subjects. Following my graduation I stayed a few months as a Postdoctoral Researcher at Cornell University. In April of 2008 I started my current


position as a Postdoctoral Research Scientist at Columbia University Medical Center. At Columbia University I switched gears to study Gram Positive Infectious Diseases, specifically MRSA and its outer membrane adhesion proteins involved in infections. In my current position I have expanded and acquire new molecular biology skills and have the opportunity of collaborating across different field of clinical research. Recently I am interested in clinical microbiology and industry. For this reason I enrolled and completed an online CRA (clinical research associate) training. This training included Drug Development Process which includes FDA Regulations, ICH Guidelines and HIPAA as they pertain to the Monitoring of Clinical Studies and on to the Initiation of a Study, Monitoring the Study and Closing the Study. Good Clinical Practice and Standard Operating Procedures were also covered as part of the training as well in the various certifications and training offered by Columbia University Medical Center. During my ongoing career I have accumulated more than twelve years of research experience. I have been involved in projects that require training in basic and specialized laboratory techniques; protocol design and editing and research data analysis. I have also trained other laboratory personnel and coordinate a small research group while participating in interdepartmental projects. For a greater detail on my background, skills and my contact information please read my attached CV.


Faustino Andreas Toba Francis, PhD. Home Contact Information: 715 W 172nd Apt 64 New York, NY 10032 Phone: (607) 280-2300 E-Mail: fat2108@columbia.edu

Office Contact Information: 630 W 168th P&S 9-458 Division of Infectious Disease Columbia University, New York, NY 10032

Current Position POSTDOCTORAL RESEARCH SCIENTIST, COLUMBIA UNIVERSITY, NEW YORK, NY Department of Medicine, Division of Infectious Diseases, 2008Present

Education PhD. IN MICROBIOLOGY, CORNELL UNIVERSITY, ITHACA, NY Department of Microbiology, 2002-2008 Environmental Microbiology and Toxicology Lab Major: Microbiology. Minors: Biochemistry and Toxicology Dissertation: Characterization of the Physiological Implications of Defective Lambdoid Phages DLP12 in E. coli. Advisor: Dr Anthony G. Hay. Committee Members: Dr. Eugene Madsen. Dr. David Wilson Additional committee members: Dr. Esther Angert. Dr. James Shapleigh


Bachelor in Science (with Honors), UNIVERSIDAD CENTRAL DE VENEZUELA (UCV), CARACAS, VENEZUELA Escuela de Biología, Facultad de Ciencias 1994-2000 Bacterial Plasmids Lab Major: Biology (Honors 16.3 (20 max)). Specialty: Cellular Biology Undergraduate Thesis: Caracterización de plásmidos bacterianos en cepas aisladas en el Embalse Pao-Cachinche. (Bacterial Plasmid Characterization from Strains Isolated from Pao-Cachinche Water Reservoir) Advisor: Dr Guillermina Alonso Examiners: Dr. Vidal Rodriguez-Lemoine, Dr. Antonieta Porco Major: Biology (Honors). Specialty: Cellular Biology

Languages English and Spanish: Speak, read and write (Bilingual) Portuguese: Reading proficiency, basic conversation

Awards and Honors 2002 Acceptance and Scholarship for Microbiology Ph D. program at Cornell U. 2002 Orinoquia Award for Applied Investigation, Orinoquia Foundation 2001 Graduation Honor Award –First place in Class and best GPA. School of Biology, Science College, Universidad Central de Venezuela (UCV)


Research Experience Working in a laboratory environment since 1995 (+14 year experience) School of biology, universidad central de venezuela (dr. Alonso laboratory) 1995-2002 Microbiology deparment, cornell university (dr. Hay laboratory) 2002-2008 Deparment of medicine, columbia university (dr. Lowy laboratory) 2008-present

Advisor Responsibilities Trained three undergraduate students in the Molecular and Microbiology techniques used in lab research. Trained one graduate student in Molecular and Microbiology techniques used in lab research. Collaborated with several graduate students in projects that require Molecular and Microbiology techniques used in lab research. Collaborated with two Post Doctoral fellows in projects that require Molecular and Microbiology techniques used in lab research. Assisted in the coordination of a research group compose of one lab technician and two undergraduates.


Research Techniques Molecular Biology and Laboratory Equipment Basic Techniques Sterilization Luminometric measurement PCR FACS DNA and RNA extraction Enzyme assays Protein expression and Biofilm research related assays extraction HLPC Bacterial culturing Accelerated Soil Extraction Viable Cell count Certified for Radiation Work Mutant generation GS Chromatography DNA cloning General Gene Cloning Primer design Western-Blot Spectrophotometric ELISA measurements Protein Assays Fluorimetric measurements Cellular Biology Tissue Culture Human CaCo2 cells Microscopy General Light Microscopy Deconvolution Microscopy Confocal Microscopy Image Capturing and Analysis General SlideBook deconvolution microscopy software Leica confocal microscopy software

HeCat cells

Transmission Electron Microscope

COMSTAT image analysis software Image J software


Other General Animal modeling

Professional Experience 2008-Present Post Doctoral Research Scientist, Department of Medicine, Columbia University 2002- 2008 Graduate Research Assistant, Department of Microbiology, Cornell University 2002

Research Assistant in Petroleum Biology Lab, Institute of Advance Studies, Venezuela (IDEA).

2001-2002 Research Assistant in Bacterial Plasmid Lab, Institute of Experimental Biology, Universidad Central de Venezuela. 1996-2000 Undergraduate Research Assistant Bacterial Plasmid Lab, Institute of Experimental Biology Universidad Central de Venezuela. 1995

Internship in the Plant Ecology Lab., Botanical Institute, Caracas Botanical Garden, Universidad Central de Venezuela.


Training and Certifications Laboratory Safety and Hazardous Waste Management Training 2008 Columbia University The Safe Use of Formaldehyde/Xylene (NEVIS) 2008 Columbia University HIPAA: Health Insurance Portability Accountability Act Research Training Course 2008 Columbia University HS GCP (Good Clinical Practice) - Patient/Clinical 2008 Columbia University Bloodborne Pathogens Training 2008 Columbia University Animal training (Mice) 2008 Columbia University Monitoring Clinical Studies (CRA Training) (Palm Coast, Fl) Training completed in 2007 (Issued by Clinical Research Training Online Inc.)

Radiation Training (Cornell University, Ithaca NY) Training completed in 2004 Issued by the New York State Department of Health BSL2 Safety Training (Cornell University, Ithaca NY) Training completed in 2007 Electron Microscopy Training (Cornell University, Ithaca NY) Training completed in 2005


Confocal Scanning Laser Microscopy (Cornell University, Ithaca NY) Training completed in 2005

Other Professional Experience 2004-2005 Vice President Field of Microbiology Students (FOMS) at Cornell University Responsibilities: Coordinate and host several academic and social events. Plan prospective student visits. Edit the FOMS student handbook 2003-2008 President of the Venezuelan Association at Cornell. Responsibilities: Coordinate and host several cultural, social and fund raising events. Design the current by-laws.

Other Skills Computer software General Windows Mac OS X Internet browsing software Microsoft Office

Adobe Acrobat Professional Adobe Photoshop Power Point Microsoft Excel


Teaching Experience 2005-2007 Small Group Discussion Leader, General Microbiology at Cornell University(BIOMI 290) Enrollment: 12 students (Student evaluations available upon request) 2005, 2007 Teaching Assistant in General Microbiology Lab course at Cornell University (BIOMI 291) Enrollment: 18 students (Student evaluations available upon request) 2001

Teaching Instructor in Cell Biology Lab course, College of Science, Universidad Central de Venezuela. Enrollment: 32 students

1998-2000 Teaching Assistant in Genetics Lab course (3 semesters), College of Science, Universidad Central de Venezuela. Enrollment: 16 students 1998-1999 Teaching Assistant in Microbiology Lab Course (2 semesters), College of Science, Universidad Central de Venezuela. Enrollment: 16 students

Publications Arrecubieta C, Toba FA, von Bayern M, Akashi H, Deng MC, Naka Y, et al. SdrF, a Staphylococcus epidermidis Surface Protein, Contributes to the Initiation of Ventricular Assist Device DrivelineRelated Infections. PLoS pathogens. 2009 May;5(5):e1000411 Junker, L. M., F. A. Toba, and A. G. Hay. 2007. Transcription in Escherichia coli PHL628 biofilms. FEMS Microbiology Letters 268:237-243.


Toba, F. A., and A. G. Hay. 2005. A simple solid phase assay for the detection of 2,4-D in soil. Journal of Microbiological Methods 62:135-143. Guillermina Alonso, Patricia Narváez, Faustino Toba, Carlos Gomes, Raquel Pedroza y Vidal Rodríguez-Lemoine. 2001. Caracterización de plásmidos de bacterias procedentes de diferentes ambientes de Venezuela. Memorias del Instituto de Biología Experimental vol. 3: #1: 93-96 (2001).

Conference Presentations 2009 Poster presentation: “Role of biofilm in Staphylococcus aureus and Staphylococcus epidermidis driveline infections, an in vivo model” Gordon Research Conferences: Assisted Circulation. Waterville Valley, New Hampshire, USA 2009 Poster presentation: “Further Characterization of SdrF, a Staphylococcus epidermidis Protein Involved in Ventricular Assisted Device Infections.” American Society for Microbiology 109th General Meeting, Philadelphia, Pennsylvania, USA 2007 Oral Seminar presentation: “Role of Defective Prophage in Biofilm Formation” North Eastern Microbiologists: Physiology, Ecology and Taxonomy (NEMPET), Blue Mountain Lake, New York, USA 2007 Poster presentation: “Role of Cryptic Lambdoid Phage Antiterminators in Biofilm and Stationary Phase Lifestyle of Escherichia coli” North Eastern Microbiologists: Physiology, Ecology and Taxonomy (NEMPET), Blue Mountain Lake, New York, USA 2007 Poster presentation: “Role of Cryptic Prophage in Biofilm Formation”


North Eastern Microbiologists: Physiology, Ecology and Taxonomy (NEMPET), Blue Mountain Lake, New York, USA 2007 Poster presentation: “Role of Cryptic Prophage in Biofilm Formation” American Society for Microbiology 4th Biofilm Conference, Quebec City, Canada 2006 Poster presentation: “Cryptic prophage genes affects E. coli Biofilm.” American Society for Microbiology 106th General Meeting, Atlanta, Georgia, USA 2005 Poster presentation: “gspM (pshM) affects E. coli Biofilm Formation.” North Eastern Microbiologists: Physiology, Ecology and Taxonomy (NEMPET), Blue Mountain Lake, New York, USA 2005 Poster presentation: “gspM (pshM) affects E. coli Biofilm Formation.” American Society for Microbiology 105th General Meeting, Atlanta, Georgia, USA 2004 Poster Presentation: “Role of Phage-related Genes on Biofilm Development” North Eastern Microbiologists: Physiology, Ecology and Taxonomy (NEMPET), Blue Mountain Lake, New York, USA 2004 Poster Presentation: “Role of Phage-related Genes on Biofilm Development” American Society for Microbiology 104th General Meeting, New Orleans, Louisiana, USA 2003 Attendance to North Eastern Microbiologists: Physiology, Ecology and Taxonomy (NEMPET), Blue Mountain Lake, New York, USA 2003 Attendance to American Society for Microbiology 103rd General Meeting, Washington, DC, USA 2001 Poster presentation: “Plasmid characterization from different environments in Venezuela.”


Institute of Experimental Medicine Annual Meeting (IME-UCV), Caracas, Venezuela 1999 Poster: “Resistencia Múltiple a Antibióticos y Metales Pesados en Plásmidos Identificados en Bacterias aisladas del Embalse PaoCachinche, Venezuela.” 49a Convención Anual de la Asociación Venezolana para el Avance de la Ciencia (ASOVAC) Maracay, Venezuela 1999 Poster presentation: “Multidrug and Metal Resistant Bacteria in a Venezuelan Water Reservoir”. American Society for Microbiology 39th Interscience Conference on Antimicrobial Agents and Chemotherapy (ASM-ICAAC), San Francisco California, USA.

Professional Membership American Society for Microbiology (ASM) (since 1999)

Other Interests Enjoy reading, movies, cooking, writing, event planning and coordination as well as group activities, several games and hobbies. Sports: General interest with special interest in Soccer, Basketball and Baseball.


References William C. Ghiorse, Ph. D. Department Chair

David B. Wilson, Ph. D. Professor

Department of Microbiology Cornell University B75C Wing Hall Ithaca, NY 14853-5701 Phone: (607) 255-3086 Fax: (607) 255-3904 E-Mail: wcg1@cornell.edu

Department of Biochemistry Cornell University 458 Biotechnology Building Ithaca, NY 14853-5701 Phone: (607) 255-5706 E-Mail: dbw3@cornell.edu

Anthony G. Hay, Ph. D. Associate Professor Department of Microbiology Cornell University B53A Wing Hall Ithaca, NY 14853-5701 Phone: (607) 255-8471 Fax: (607) 255-3904 E-Mail: agh5@cornell.edu

Others references available upon request

Faustino A. Toba, PhD  

Curriculum Vitae

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