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Appendix-III Method for p53 immunostaining Fixation: 10% formal saline. Processing: First the tissue is fixed in 10% formal saline for 24 hours. Then the tissue is dehydrated through different concentration of alcohol. Then the tissue is cleared by xylene followed by paraffin impregnation at 56 degree C for one hour. Then preparation of paraffin block. Paraffin wax, with xylene, as the clearing agent. Sections: Sections are cut by a microtome between 3 and 5 microns in thickness. Mount on poly-L-lysine coated slides. Dry at 37째C for overnight is preferable. Pretreatment: 1. Dewax sections in xylene 5 mins. 2. Rinse in a fresh bath of xylene. 3. Rinse in 2 baths of absolute alcohol. 4. Place the slides in pre warm Tris-EDTA (pH 9) solution containing coupling jar. The jar is then placed in a water bath at 98 degree C for 20 minutes. 5. The coupling jar is then kept in room temperature and when the internal temperature become equal to the room temperature, then the slides are taken out of Tris-EDTA solution and ringed in water followed by tris buffer. Staining: 1. Incubate the slides in peroxidase block (Dako) for 15 mins. 2. Ringe in tris buffer and drain off excess fluid and wipe off by lint free tissue. 3. Incubate in primary antibody (Dako lot no S-2144) for 30 minutes 4. Ringe in tris buffer and drain off excess fluid and wipe off by lint free tissue. 5. Incubate in labeled polymer for 30 minutes.

Thesis on oral cancer and bangladesh  

The term oral cancer encompasses all malignancies that originate in the oral tissues. Squamous cell carcinoma of the oral cavity comprises 9...

Thesis on oral cancer and bangladesh  

The term oral cancer encompasses all malignancies that originate in the oral tissues. Squamous cell carcinoma of the oral cavity comprises 9...