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p53 and 7 cases were negative. In other words 88% showed positive expression and 12% showed negative. All OSCC are not p53 positive according to most of the literatures. Rana et al. (1993, p. 147) found 75% positive cases (24 out of 32 cases). Our series showed 88% positivity which corresponds to the report of Alison, Duangpron & Peter (1999, p. 103) which had 94% positivity . All the study subjects were classified into 3 major histological groups i.e. well differentiated, moderately differentiated and poorly differentiated. These were counted as grade-I, II & III respectively. Verrucous Squamous cell carcinoma is considered as very well differentiated carcinoma. One of our aim was to find out the pattern of p53 protein in different histological grades. Here the pattern means expression, intensity and distribution of staining of p53 with monoclonal antibody. We found that 86% (24 out of 28) of the total well differentiated carcinoma were positively stained for p53. Ninety-five percent (18 out of 19) of moderately differentiated carcinoma, 86% (6 out of 7) of poorly differentiated and 80% (4 out of 5) of verrucous carcinoma had positive expression. Famulski et al. (2002, p. 206) & most of the other studies revealed that there was no direct correlation between p53 expression and grading of the tumor. This indicates that the p53 over expressed tumor probably do not have much more aggressive behavior than those without over expression (Yan et al. 1995, p. 138). This information favors the finding of our study regarding this variable. On the contrary Jasbir, Anurag & Ranju (1998, p. 370) tried to show a relationship between these two parameters where well differentiated carcinoma showed 53%, moderately differentiated 77% and poorly differentiated showed 84% positive p53 expression. When we analyzed the intensity of the staining of p53 and its correlation with histological grading or differentiation, we found the relation statistically significant (p=0.012). The intensity of staining was assessed in the cases by reference to photographic standard in a semi quantitative manner as weak (w), moderate (m) and strong(s), which were also expressed as weak (+), moderate (++) and strong (+++). The result from the present study indicated that p53 abnormalities in the OSCC as detected by the intensity of the immunohistochemical staining increased from verrucous type to poorly differentiated one. There was a very little evidence showing agreement with our study regarding the relation between the intensity pattern and the grading of the OSCC. However the result of our study appeared to be similar to the findings of Hideo et al. (2005, p. 602) where a significant association between intensity of immunostaining of p53 and histological grading of malignancy was found. It was observed that when immunostaining was done for p53 protein detection, nuclear staining was confined to the suprabasal cells in premalignant lesion (Isabel et al. 1998, p. 360). In SCC, positive p53 staining was found equally in all basal, parabasal and spinous cells layer (Jang Jaer et al. 2005, p. 471). Findings of the distribution of p53 in OSCC, the result of our study correlates with the result of other studies. In our study all types of OSCC other than verrucous type showed distribution

Thesis on oral cancer and bangladesh  

The term oral cancer encompasses all malignancies that originate in the oral tissues. Squamous cell carcinoma of the oral cavity comprises 9...

Thesis on oral cancer and bangladesh  

The term oral cancer encompasses all malignancies that originate in the oral tissues. Squamous cell carcinoma of the oral cavity comprises 9...