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Australian Plant Phenomics Facility

The Plant Accelerator速

Use of The Plant Accelerator速 for high-throughput phenotyping Mark Crowe October 2011

www.plantaccelerator.org.au


Australian Plant Phenomics Facility

The Plant Accelerator®

The Plant Accelerator • • • • •

34 Greenhouses 4 Smarthouses 2 Imaging suites 13 Growth chambers Laboratory and offices

• >75 projects completed • >1,500 plants in Smarthouses

www.plantaccelerator.org.au


Australian Plant Phenomics Facility

The Plant Accelerator速

The Team

www.plantaccelerator.org.au


Australian Plant Phenomics Facility

The Plant Accelerator速

Drought and salt tolerance Trait dissection in cereals

www.plantaccelerator.org.au


Australian Plant Phenomics Facility

The Plant Accelerator®

Salt tolerance and salinity • >80% of Australian cereal cropping region is at risk of being affected by salinity – Rengasamy, 2006

• Salinity costs the Australian economy as much as $1.3b annually – CRC for Soil and Land Management, 1999

www.plantaccelerator.org.au


Australian Plant Phenomics Facility

The Plant Accelerator速

Salinity damaging effects Osmotic effects

Ionic effects

Munns and Tester (2009) Annu.Rev. Plant Biol. 59: 651-681

www.plantaccelerator.org.au


Australian Plant Phenomics Facility

The Plant Accelerator速

Variation in osmotic tolerance

Nawar Shamaya, ACPFG

www.plantaccelerator.org.au


Australian Plant Phenomics Facility

The Plant Accelerator®

Salt tolerance hints and tips • Salt addition • Concentration • Calcium supplementation

www.plantaccelerator.org.au


Australian Plant Phenomics Facility

The Plant Accelerator®

Drought tolerance • Drought can more than halve Australia’s wheat production • National average yield ~2t/ha

www.plantaccelerator.org.au


Australian Plant Phenomics Facility

The Plant Accelerator速

Components of Drought Tolerance

Berger B et al. J. Exp. Bot. 2010;61:3519-3528

www.plantaccelerator.org.au


Australian Plant Phenomics Facility

The Plant Accelerator速

3,E+05

Analysing drought response Leaf blade area [mm2]

3,E+05

Barley2,E+05 lines grown under well 2,E+05 watered and drought (-5 bar) 1,E+05 conditions 5,E+04

2,E+05 2,E+05 1,E+05 5,E+04 0,E+00

0,E+00 25

Relative growth rate

35

45

55

65

75

0,16

[d-1]

[d-1]

3,E+05

0,14 0,12 0,1 0,08 0,06 0,04 0,02 0 25

35

45

55

65

75

Time after sowing [d] Clipper drought Clipper well watered

Relative growth rate

Leaf blade area [mm2]

3,E+05

25

35

45

55

65

75

25

35

45

55

65

75

0,16 0,14 0,12 0,1 0,08 0,06 0,04

0,02 0

Time after sowing [d] Sahara drought Sahara well watered Boris Parent - ACPFG

www.plantaccelerator.org.au


Australian Plant Phenomics Facility

The Plant Accelerator®

Drought tolerance hints and tips • Level of drought • Calibration • Non-measurable traits

www.plantaccelerator.org.au


Australian Plant Phenomics Facility

The Plant Accelerator®

Replacement of destructive testing • Biomass measurement • Chlorophyll content

www.plantaccelerator.org.au


Australian Plant Phenomics Facility

The Plant Accelerator®

• Works for various plant types

Projected shoot area [pixel]

– Wheat, barley, rice, maize 6,E+05 5,E+05 4,E+05

y = 5,4028x + 15951 R² = 0,9605

3,E+05 2,E+05

Projected shoot area [pixel]

Using ‘projected shoot area’ as a measure for plant size and biomass 6,E+05

y = -5340,7x2 + 97002x + 6824,7 R² = 0,9661

5,E+05 4,E+05 3,E+05 2,E+05 1,E+05 0,E+00 0

2

4

6

8

10

Dry weight [g]

1,E+05 0,E+00 0,E+00

2,E+04

4,E+04

6,E+04

8,E+04

Leaf blade area [mm2]

1,E+05

• Project underway with – Cassava, chickpea, sorghum, sugarcane, plus several others B Parent – ACPFG & B Berger – The Plant Accelerator

www.plantaccelerator.org.au


Australian Plant Phenomics Facility

The Plant Accelerator®

Colour as a indicator of chlorophyll content y = 2.366x + 1.0883 R² = 0.8315

0,6 0,5 0,4

4,0

0,3

y = 0.2261x - 9.6447 R² = 0.8129

3,5 0,2 0,1 0,0 0

0,1

0,2

0,3

0,4

0,5

0,6

Relative 'dark green' area of leaves

0,7

Total Chlorphyll content [mg/g]

Total Chlorophyll content [mg/g]

0,7

0,8

3,0 2,5 2,00,9 1,5 1,0 0,5 0,0 46

48

50

52

54

Mean hue of sideview images

www.plantaccelerator.org.au

56

58


Australian Plant Phenomics Facility

The Plant AcceleratorÂŽ

Growth rate case study • The real value of high-throughput, highresolution, non-destructive phenotyping

www.plantaccelerator.org.au


Australian Plant Phenomics Facility

The Plant Accelerator速

Growth rate analysis

Rhiannon Schilling, University of Adelaide

www.plantaccelerator.org.au


Australian Plant Phenomics Facility

The Plant Accelerator速

Growth rate analysis

Rhiannon Schilling, University of Adelaide

www.plantaccelerator.org.au


Australian Plant Phenomics Facility

The Plant Accelerator速

Growth rate analysis

Rhiannon Schilling, University of Adelaide

www.plantaccelerator.org.au


Australian Plant Phenomics Facility

The Plant Accelerator速

www.plantaccelerator.org.au


Australian Plant Phenomics Facility

The Plant Accelerator速

www.plantaccelerator.org.au


Use of The Plant Accelerator for high-throughput phenotyping