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PhenoFab Europe


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Trait Spotting – our Business In the plant breeding industry phenotyping is one of the most crucial aspects of variety development. Phenotyping is laborious and requires significant experience and a professional breeder’s eye. The high complexity of agronomically important traits makes it even more difficult to perform objective and robust phenotyping in a high throughput manner. In contrast, the enormous accomplishments that have been achieved in highthroughput, sequence-based plant genotyping emphasize the need to scale up the development of automated phenotyping technology. PhenoFab™ is a greenhouse service operation that combines high-throughput, non-invasive technology with trait interpretation to exploit phenotypic variation. It closes the “phenotyping gap”.

The Fab – our Facility The PhenoFab™ services are delivered with a robust phenotyping platform in a greenhouse setup. Determination of the phenotype is based on moving pots or trays with plants through a greenhouse compartment and fixed scanning areas containing image technology to capture digital data. The plants grow in individual containers/tray-wells and are scanned at preset time points from various angles using VIS (visible light), NIR and fluorescent imaging technology. The facility has climate-controlled compartments that allow plants to grow up to 2.6 m under different environmental conditions, enabling experiments with different growth or stress factors. Depending on the plant size and experimental setup the capacity will vary around an average of 1,100 plants.


The Technology – a wide Spectrum

Digital trait analysis is based on visible light and NIR (near infrared spectrum) imaging as well as fluorescent imaging. RGB images are analysed using pixel values to identify shape, colour and other morphological digital phenotypes. The NIR-infrared images are specifically employed to reveal internal structures of plants, water content or other (e. g. chemical) compositions. Lastly, the fluorescence imaging allows GFP-protein and chlorophyll analysis to be performed. Combinations of all imaging technologies can be used to resolve objects that have low variability in any one spectrum.

The Service – Specifications The facility for the PhenoFab™ service provides: n

More than 300 m2 modern greenhouse space (PK2 certified) allocated for phenotyping

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Objective phenotyping studies in controlled environments, using a dedicated greenhouse with specific hardware

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Digital phenotyping combined with specifically designed statistical approaches developed for different type of Crops

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Trait analysis on e. g. colour, shoot & biomass development, heritability, root development, canopy structure, as well as (a)biotic stress analysis

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Multilevel digital trait observations of individual plants (e. g. angles, time series, light filtering)

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Proprietary software tools for image-to-digital phenotype and statistical analysis

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Completely integrated image and result database to store experimental data (similar to LIMS)

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Water/nutrient supply controlled at individual plant level


Concept & Design by www.v-werk.de

Arjen J. van Tunen CEO Keygene N.V.

Dirk Vandenhirtz CEO LemnaTec GmbH

We do the PhenoFab It’s a Green Gene Revolution

Scientific image processing since 1998

KeyGene delivers sustainable molecular genetic responses to the world’s need for stability in the yield, quality and health of crops. It’s our passion to explore and exploit genetic variation in vegetables and other 6F crops (Food, Feed, Fiber, Fuel, Flowers and Fun crops). For more than 20 years, we assist breeding companies by providing cutting edge molecular breeding technology and trait improvement platforms for their own crop development. Therefore KeyGene has invested strongly in next generation high-throughput Phenotyping technology to accelerate new applications for breeding. Using this advanced digital technology makes it possible to accelerate linkage of complex traits to the genetic variation.

Founded in May 1998 in Aachen (Germany) with 8 partners coming from different backgrounds LemnaTec’s philosophy has always been to consolidate various competences. The interdisciplinary LemnaTec team combines biologists, physicists, chemists, bioinformaticans, ecotoxicologists as well as hard- and software engineers.

KeyGene has its headquarters in Wageningen, the Netherlands, a subsidiary in Rockville, Maryland, USA and a Joint Lab at the Shanghai Institute of Biological Sciences in Shanghai, China. Contact: info@keygene.com · Phone: +31 317 466 866

The LemnaTec team of scientists develops hard- and software solutions for plant phenomics, high-throughput and high-content screening of plants, seedlings, insects and other organisms and for the automated evaluation of bio tests in ecotoxicology. Digital images are primarily taken by the scanalyzer3D and scanalyzerHTS systems all set up in a modular design. Using advanced LemnaTec image processing algorithms and fully integrated data analysis, every visible parameter (e. g. colour, shape, size, and architecture) is subsequently measured and further processed to be correlated with experimental records (e. g. genetic data). Our aim: to visualise and analyse the biology beyond human vision. Contact: info@lemnatec.com · Phone: +49 2405 412 600

Contact us! PhenoFab™ Europe Agro Business Park 90 6708 PW Wageningen The Netherlands

Phone: +31 317 466 866 Fax: +31 317 424 939 Email: info@phenofab.com Web: www.phenofab.com

www.phenofab.com


PhenoFab by lemnaTec and Keygene