Role of plant-based bio-products in combating antimicrobial resistance on biomedical devices Vartika Srivastava and Rakhi Chaturvedi
Indian institute of Technology Guwahati, Assam, India
The surge of antimicrobial infections have extremely wide and so their inhibitory strategies, too. The implantable biomedical devices or invasive devices such as endoscope develop an antibiotic resistant biofilm community on their surfaces. The present study aims a strategy of utilizing in vitro plant based bioactive compounds in the form of nanogel to be applied on the surfaces of the devices. The plausible mechanism could be inhibition of biofilm formation and their killing.
RESULTS AND DISCUSSION
Generation of in vitro callus lines using leaf-discs
Plant material
% Relative abundance
ABSTRACT
RATIONALE m/z
Development of nanogel formulation of target bioactive isolated drug against antibiotic resistance induced on usage of biomedical device surfaces. METHODS
MIC determination for target metabolites Nanogel enclosing purified metabolites
Isolation and characterization of bioactive compounds using HPLC and MS Pore formations in bacterial cell
Generation of in vitro cell lines of target medicinal plant Isolation and characterization of bioactive compound Speculation of inhibitory and killing Application of nanogel on concentrations of compound Generation of nanogel formulation to be Confirmation of antimicrobial action biomedical devices applied on biomedical device using fluorescence microscopy
CONCLUSION
In vitro cell lines were developed on MS medium and the key metabolites were purified and characterized using HPLC and MS. MIC and killing % was found out to be 85% as confirmed by fluorescence studies as well. Bacterial cell lysis and pore formation was observed via FESEM study. The purified compounds would be made in the form of nanogel to be applied on biomedical devices, as future endeavor. The authors thank IIT Guwahati for providing facilities for carrying out research work.