Kokilavani.R,
Department Of Biotechnology St. JOSEPH'S COLLEGE OF ENGINEERING, OMR Road Chennai-119
ABSTRACT Nanotechnology is an expeditious developing field which is based on the construction of functional materials, system and devices etc. These devices and system are been controlled by atoms on the nanoscale which express a novel properties in the particular size. AgNPs doped with hydroxylapatite has gained wide attention in dental implant coating due to its outstanding properties such as biocompatibility, non-toxicity and anti-microbial. In ayurvedic medicine, Syzygium aromaticum is used as an analgesic for dental emergencies. In this study, AgNPs was biological synthesized using E. coli grown in M9 media. The synthesized AgNps was doped with nanohydroxyapatite obtained from eggshell and the doped material was further added to the extract of Syzygium aromaticum. The synthesized composite was characterized using SEM and EDAX to study about the size and elemental composition of the composite. The presence of organic and inorganic compounds and its structural nature was studied using FTIR and XRD. The anti-microbial activity against bacteria and fungi was studied using the synthesized composite. The anti-adhesion, antiinflammation, compressive, hardness test and surface properties studied too confirmed its nature and can be used in the dental applications.
OBJECTIVE
INCORPORATION OF AgNPs, nHAP AND Syzygium aromaticum EXTRACT
ANTIMICROBIAL ACTIVITY
The AgNPs were characterized using UV–visible spectroscopy as a result a broad peak located at 420 - 440 nm was observed for the AgNPs prepared using the E.coli
PREPARATION OF SILVER NANOPARTICLES
ANTI-ADHESION ACTIVITY EDAX STUDY
The results showed that 90% of anti-adhesion. The adhered cells were inhibited after 3 h of contact between E.coli and the nano composite. This shows ability of nano composite to inhibit adhered cells, preventing the further formation of mature biofilms and can be used in dental applications
COMPRESSIVE STRENGTH Sample
PREPARATION OF NANOHYDROXYAPATITE
X-RAY DIFFRACTION STUDY
Compressive Stength (MPa)
Control (N-HAP) Nano composite
Average Value (MPa)
Standard Deviation (MPa)
41
42
45
42.67
0.8
52
50
48
50.33
1.3
HARDNESS TEST SL.NO
PROPERTY
RESULT
1
Hardness, shore D
65-70%
The hardness of the nanocomposite was with in time and reproducibility result was good, therefore the composite can be used as a dental material
FTIR STUDY
1000
Neat
0.194
2
500
1:1
0.280
52.04
3
250
1:2
0.341
63.38
4
125
1:4
0.402
74.72
5
62.5
1:8
0.456
84.75
6
31.2
1:16
0.512
95.16
7
15.6
1:32
0.569
100
8
7.8
1:64
0.622
100
9
Cell control
-
0.538
100
CONCLUSION The synthesized silver nanoparticles, nano hydroxyapatite and the composition was characterized by UV-spectrometer, SEM, EDAX, XRD and FTIR. The composition was subjected to following applications: The antimicrobial activity of the synthesized nanoparticles and nano composite was efficient. Its shows a significant anti-inflammatory potential. The nano composite shows 90 % inhibition of adhered cells. The water absorbed by the composite was found to be 4.2 % which is efficient. It shows good compressive strength as compared with nano hydroxyapatite. The hardness of the nanocomposite was with in time and reproducibility result was good.The surface of the composition was found to be similar to natural tooth, therefore can be used in dental filling.The cytotoxicity study was done on vero cell line for the composite shows 100% vaible cell as the concentration increases the percentage of the cell starts to decrease, so therefore the nano composite can be used in dental applications.
REFERENCES
TEST METHOD : SATRA TM 205 : 1999 (MODIFIED)
PREPARATION OF Syzygium aromaticum EXTRACT
1
Cell viability (%) 36.05
ANTI-INFLAMMATORY ACTIVITY HRBC membrane stabilization method was used to investigate in vitro anti inflammatory activity (Mizushima and Kobayashi et al.,1968) of nano composite. The inhibition of hypotonicity induced HRBC membrane lysis (which is the response of inflammation) stabilization was taken as a measure of the anti inflammatory activity. HRBC membrane may be considered as a model of the lysosomal membrane which plays an important role in inflammation. The composite inhibits 83.47 % of inflammation.
METHODOLOGY
Dilutions
Absorbance( O.D)
The analysis was carried out by ASTM D570.The absorbtion of the composite was 4.2%. The rate of absorption increased weight by time and the rate of absorption been constant after 3hours, Therefore this composite can be used in dental applications.
UV -VISIBLE SPECTROSCOPY STUDY OF AgNPs
To synthesize silver nanoparticles To prepare nHAP from eggshell To produce AgNP-HA/Syzygium aromaticum nanocomposite To charaterize the nanocomposite Application studies of nanocomposite
S.No
Concentration (µg/ml)
WATER ABSORPTION TEST
CHARACTERIZATION RESULTS
SEM STUDY
CYTOTOXICITY ACTIVITY
APPLICATIONS OF THE COMPOSITE
SURFACE REPLICATION OF TOOTH MATERIAL
• Iris Wing-Shan Lin, Chun-Nam Lok and Chi-Ming; Che Cite this: Chem. Sci. , 2014, 5, 3144. • Sangiliyandi Gurunathan, Kalimuthu Kalishwaralal, Ramanathan Vaidyanathan,Venkataraman Deepak, Sureshbabu Ram Kumar Pandian, Jeyaraj Muniyandi, Nellaiah Hariharan, Soo Hyun Eom; Colloids and Surfaces B: Biointerfaces 74 (2009) 328–335. • C. Karthik, V. Radha; Digest Journal of Nanomaterials and Biostructures Vol. 7, No. 3, July - September 2012, p. 1007 - 1014. • C. Karthik, V. Radha; Orient. J. Chem., Vol. 32(1), 735-741 (2016).
ACKNOWLEDGMENT
The structure of the setted composite shows a significant amount of pores was observed. The structure of the tooth (a) and the setted composition (b) was found to be similar, therefore can be used in dental filling.
I express my sincere thanks for their constant support throughout the project for the following teaching faculties members and to all lab technicians. Especially thanks to my friends and family Visalakshi. S Department of Biotechnology Periyar University, Salem Pride
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