PROBIOTICS : A GENUINE RESPONSE AGAINST COLITIS MRUTYUNJAYA PANDA1,M.Sc., JAGRUTI MISHRA2, M.Sc 1
College of pharmaceutical sciences, Mohuda, Berhampur , 2Orissa university of Agriculture and technology, Bhubaneswar
INTRODUCTION Probiotics: good bacteria are remain reside in the host organism and play an important role in the improvement of host health. Most of the probiotic products currently available contain lactic acid bacteria (LAB) which belong to the Lactobacillus and Bifidobacterium strains. These bacteria are present in human’s gut either early present or through ingestion of fermented food, they have strains with probiotic effect. Particularly Bifidobacterium, Enterococcus & Lactobasillus are most widely used probiotic bacteria. These probiotics helps to cure various disease Pathogenecity, but here we focus mainly on Ulcerative Colitis which cause is currently unidentified but hypothetically H.pylori may play an important role in inflammatory bowel disease due to their comparable immunological pathogenesis. Previous study on colon and stool of infected person showed the presence of Pylori which reside in the surface epithelium of stomach and causes inflammation. This research was conducted to investigate the antiinflammatory effect of Probiotics like Bifidobacterium to create remedy against Colitis like inflammatory disease.
ABSTRACT
OBJECTIVE
Probiotics are living organisms with adequate amt of consumption beneficial to the host. Wide range of Probiotics are found in human body as well as taken as food, dietary products, supplements and drugs. Many evidence shows that Probiotics have significant effect in alleviating symptoms of various disease like acute diarrhoea, functional gastro-intestinal disorders and inflammatory Bowel disease. Recent study implies that acute Ulcer Colitis (UC), a major inflammation of colon also somehow curable through Probiotics consumption. In this study we did some major invivo and invitro interaction between Helicobacter pylori, intestinal bacteria, which is a major cause of inflammation in colon and Bifidobacterium infantis, a probiotic bacteria to know the role of probiotics in the way of suppression of pathogenecity. Various wet lab techniques starting from the gel preparation to media preparation have been done for isolation of H. pylori in Cow dung and sludge sample and B. infantis in human baby stool and breast milk sample. Catalase test was done for both where Urea test and sugar fermentation test were done for confirmation of H. Pylori and B. Infantis respectively. This research was carried out with the afterward preparation of antibiotic resistant H.pylori and B. Infantis and their effect on one another. This research work showed interaction between the both bacteria and their effect on each other, which further opens up the pathway of study of pathogenecity and their treatment through advanced tool like probiotics.
ISOLATION OF HELICOBACTER PYLORI CONFIRMATION OF HELICOBACTER PYLORI ISOLATION OF BIFIDOBACTERIUM INFANTIS CONFIRMATION OF BIFIDOBACTERIUM INFANTIS PREPARATION OF ANTIBIOTIC RESISTANT HELICOBACTER PYLORI & BIFIDOBACTERIUM INFANTIS EFFECTS OF BIFIDOBACTERIUM INFANTIS (NORMAL & RESISTANT) ON HELICOBACTER PYLORI(NORMAL &RESISTANT)
RESULTS CONCLUSION METHODOLOGY
ISOLATION OF H.pylori H.pylori isolation is done from cow dung using H.pylori specific peptone agar (HPSPA) (Merck -1003171000). Secondary culture is done from the primary culture to avoid any other impurities. CONFORMATION OF H.pylori Conformation of H.pylori is done using catalase & urea test. ISOLATION OF B.infantis Isolation of B.infantis is done from baby stool & breast milk using MRS (De Man ,Rogosa &Sharpe ) agar media. CONFORMATION OF B.infantis Conformation of B.infantis is done using catalase test. PREPARATION OF ANTIBIOTIC RESISTANCE OF H.pylori Antibiotic resistance of H.pylori is prepared using AMOXICILILN . PREPARATION OF ANTIBIOTIC RESISTANCE OF B.infantis Antibiotic resistance of B.infantis is prepared using AMOXCILIN.
REFRERENCES
B. infantis vs. H. pylori B.infantis vs Amoxy H.pylori Amoxy res.B.infantis VS Amoxy res. H.pylori
Amoxy res. B. infantis vs. normal H. pylori
FUTUREASPECT
Probiotics treatment as an adjuvant of eradication treatment showed in the recent trials efficacy against H. pylori and/or decreased side effects of the treatment in most of the studies - but not in all. In conclusion, administration of safe probiotics as an adjuvant for the current H. pylori eradication treatment appears promising, though it still requires optimization; even in the cases in which the treatments achieve high eradication rates, probiotics may reduce side effects.
Further investigation on the mechanisms behind the direct and indirect effects of probiotics on H. pylori could help not only to better refine the type of treatment, but also may contribute to better understand some aspects of H. pylori pathogenesis.
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