FLAVONIODS REDUCES STREPTOCOCCUS MUTANS BIOFILM FORMATION BY INHIBITING SORTASE A ACTIVITY SUSHIL BHAVESH.S, LAKSHMI PRIYA.L (SECOND YEAR)
BIOTECHNOLOGY KARUNYA INSTITUTE OF TECHNOLOGY & SCIENCES(Deemed to be university),Karunya nagar,Booluvampatti,Coimbatore,TamilNadu
ABSTRACT Biofilms resistance to killing prompts the need to discover new agents that inhibit and/or eradicate these quorum sensing /microbial communities. Screening natural compounds for antibiofilm activity is becoming a promising approach. In this study we characterized the antibiofilm activity of curcumin. Curcumin is an extract of turmeric known to exhibit antimicrobial properties, a polyphenolic pro-oxidant, signifcantly decreased persistence against colistin.however, its antibiofilm activity has yet to be thoroughly investigated. We find that curcumin reduces biofilm formation. Sortase A is an enzyme responsible for the covalent attachment of Pac proteins to the cell wall in Streptococcus mutans. It has been shown to play a role in modulating the surface properties and the biofilm formation and influence the cariogenicity of S mutans. curcumin imposes membrane damage in S. aureus and morphological changes in cell wall which may possibly mediate the mechanism by which curcumin exerts its anti-biofilm activity. The aim of this study was to investigate the inhibitory ability of curcumin against S. mutans sortase A and the effect of curcumin for biofilm formation.
INTRODUCTION Sortase A is an enzyme responsible for the covalent attachment of Pac proteins to the cell wall in Streptococcus mutans. It has been shown to play a role in modulating the surface properties and the biofilm formation and influence the cariogenicity of S. mutans. Curcumin, an active ingredient of turmeric, was reported to be an inhibitor for Staphylococcus aureus sortase A. The aim of this study was to investigate the inhibitory ability of curcumin against S. mutans sortase A and the effect of curcumin for biofilm formation.
METHODS •
The antimicrobial activity of the curcumin to the S.
•
mutans and inhibitory ability of the curcumin against the purified sortase A in vitro were detected.
•
Western-blot and real-time PCR were used to analysis the sortase A mediated Pac protein changes when the S.
•
mutans was cultured with curcumin. The curcumin on the S.
•
mutans biofilm formation was determined by biofilm formation analysis.
RESULT Curcumin can inhibit purified S. mutans sortase A with a half-maximal inhibitory concentration (IC50) of (10.2±0.7)μmol/l, which is lower than minimum inhibitory concentration (MIC) of 175μmol/l. Curcumin (15μmol/l) was found to release the Pac protein to the supernatant and reduce S. mutans biofilm formation.
CONCLUSION: These results indicated that curcumin is an S. mutans sortase A inhibitor and has promising anti-caries characteristics through an anti-adhesion-mediated mechanism. REFERENCES: • Curcumin alleviates persistence of Acinetobacter baumannii against colistin(Amanjot Kaur, Prince Sharma&NeenaCapalash). • Curcumin reduces Streptococcus mutans biofilm formation by inhibiting sortase A activity (PingHuangMin WeiChen).