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Tuesday, February 12, 2013 Ruby Grand Hall Erickson Alumni Center


The WVU Office of Research and Economic Development, through the support provided by the Claude Worthington Benedum Foundation, has launched an initiative to encourage innovation and commercialization through research. The initiative, titled LIINC (Linking Innovation, Industry and Commercialization), is designed to bring faculty expertise and talent to the attention of our industry partners through networking events. This particular event focuses on bioscience and biomedical research. To our industry partners, we greatly appreciate your attendance at this event and we hope you will take this opportunity to learn about the research taking place at WVU. To facilitate new partnerships and future collaboration, this booklet contains brief abstracts of our faculty’s research activities. We strongly encourage you to contact them to learn about and discuss their research in greater detail. On behalf of our faculty, we thank you for your participation and we hope you will see us as trusted partners for continued collaboration.

Dr. Fred King Vice President for Research

Name Badge Key: Blue: Industry Gold: WVU Blue/Gold: WVU Presenter 2

ATTENDING INDUSTRIES Airgas, Inc. Airgas, Inc., through its subsidiaries, is the largest U.S. distributor of industrial, medical and specialty gases, and hardgoods, such as welding equipment and supplies. Airgas is also a leading U.S. producer of atmospheric gases, carbon dioxide, dry ice, and nitrous oxide, one of the largest U.S. distributors of safety products, and a leading U.S. distributor of refriderants, ammonia products, and process chemicals.

Allegheny Science & Technology Corporation Allegheny provides Management and Technology solutions for federal, state, and local governments, federal civilian agencies, and commercial clients. We work in areas such as project management, financial analysis, software and systems engineering, as well as training and simulation, and specialized scientific, engineering and technical support.

BioWV The purpose of the Bioscience Association of West Virginia is to promote the bioscience industry in West Virginia, expand the knowledge and expertise of West Virginia’s businesses concerning the life sciences through seminars, educational publications and to enhance public awareness of the biotechnology industry in West Virginia. BioWV represents the interests of the bioscience industry in West Virginia before federal, state and local legislators and regulators.

Blanchette Rockefeller Neurosciences Institute The Blanchette Rockefeller Neurosciences Institute is a unique, independent, non-profit institute dedicated to the study of both memory and memory disorders. BRNI is operated in alliance with West Virginia University in Morgantown, as well as in collaboration with other academic institutions.

Canterbury Road Partners Canterbury Road Partners pull innovations out of universities and laboratories to launch early stage ventures. We accelerate technology transfer, campus entrepreneurship and the formation of local entrepreneurial ecosystems. To do that, we form public private partnerships between public research institutions, entrepreneurs, investors, and industry. We also provide real-world experiential learning opportunities to students interested in technology and entrepreneurship.


Celsense, Inc. Celsense is a privately held biotechnology company offering MRI agents that allow scientists and clinicians to monitor the location and quantity of transplanted cells. The company’s products have application in the fields of regenerative medicine, immunotherapy, and the study of inflammation and immune system response. Celsense is currently collaborating with leading pharmaceutical and biotechnology organizations and is developing products suitable for human use.

Defense Threat Reduction Agency (DTRA) DTRA is the U.S. Department of Defense’s official Combat Support Agency for countering weapons of mass destruction. Our people are Subject Matter Experts on WMD, and we address the entire spectrum of chemical, biological, radiological, nuclear and high yield explosive threats. DTRA’s programs include basic science research and development, operational support to U.S. warfighters on the front line, and an in-house WMD think tank that aims to anticipate and mitigate future threats long before they have a chance to harm the United States and our allies.

Eli Lilly Lilly makes medicines that help people live longer, healthier, more active lives. We were founded by Eli Lilly in 1876, and are now the 10th largest pharmaceutical company in the world. Across the globe, Lilly has developed productive alliances and partnerships that advance our capacity to develop innovative medicines at lower costs.

Federal Bureau of Prisons The Federal Bureau of Prisons was established to provide more progressive and humane care for Federal inmates, to professionalize the prison service, and to ensure consistent and centralized administration of the 11 Federal prisons in operation at the time. Today, the Bureau consists of 119 institutions and is responsible for the custody and care of approximately 219,000 Federal offenders. The Bureau protects public safety by ensuring that Federal offenders serve their sentences of imprisonment in facilities that are safe, humane, cost-efficient, and appropriately secure.

IBM IBM has a strong tradition of research collaboration with academia. We go beyond the boundaries of our IBM labs to work with colleagues in universities around the world. We also foster collaborative relationships through fellowships, grants and funding for programs of shared interest.


Intermed Partners Intermed Partners is an experienced, full service, accelerator specifically designed to assist practicing health professionals develop innovations. We utilize focused intellectual mining to discover, build, and deliver disruptive technology to a successful exit.

Illumina, Inc. At Illumina, we apply innovative technologies for studying genetic variation and function, making studies possible that were not even imaginable just a few years ago. These revolutionary tools for DNA, RNA, and protein analysis are enabling rapid advances in disease research, drug development, and the development of molecular tests in the clinic.

MBL International Corporation Established in 1993, MBL International Corporation (MBLI) is a leading life science company focused on providing high quality, innovative, solutions-based products for both life science research and clinical diagnostics. Our products are used widely in academic research institutions, pharmaceutical and biotechnology companies, government agencies as well as hospital and reference laboratories.

Mylan Pharmaceuticals Mylan is one of the world’s leading generics and specialty pharmaceutical companies, providing products to customers in approximately 150 countries and territories. The company maintains one of the industry’s broadest and highest quality product portfolios, which is regularly bolstered by an innovative and robust product pipeline.

New England BioLabs New England BioLabs is a recognized world leader in the discovery, development and commercialization of recombinant and native enzymes for genomic research. Created “by scientists for scientists,” NEB is renowned for consistently providing exceptional product quality and unsurpassed technical support. For four decades, NEB has been shaping the landscape of bioscience research by discovering, developing and supporting superior research reagents. They also offer the largest selection of recombinant and native enzymes for genomic research.

NIOSH NIOSH provides national and world leadership to prevent work-related illness, injury, disability, and death by gathering information, conducting scientific research, and translating the knowledge gained into products and services, including scientific information products, training videos, and recommendations for improving safety and health in the workplace.


Northrop Grumman Northrop Grumman Corporation is a leading global security company providing innovative systems, products and solutions in unmanned systems, cyber security, C4ISR, and logistics and modernization to government and commercial customers worldwide that are critical to the defense of the nation and its allies. From working with the Navy to developing training systems for new weapons platforms to providing public health informatics support to the CDC to managing IT infrastructure and secure wireless communications networks in states and municipalities nationwide, Northrop Grumman is meeting the needs of programs and enterprises that touch the daily lives of people everywhere. Through the integration of health expertise and information technology, Northrop Grumman Health IT delivers Innovative Health Systems and Advanced Analytics leading to Better Health Outcomes.

Novartis Novartis is a world leader in the research and development of products that protect and improve health and well-being. It’s core businesses are pharmaceuticals, vaccines, consumer health, generics, eye care and animal health.

Office of United States Senator Joe Manchin U.S. Senator Joe Manchin (D-W.Va.) was sworn into the United States Senate on November 15, 2010 to fill the seat left vacant by the late Senator Robert C. Byrd. As a Senator, Joe Manchin is committed to bringing this same spirit of bipartisanship to Washington. As he has done throughout his entire life, he remains committed to working with Republicans and Democrats to find commonsense solutions to the problems our country faces and is working hard to usher in a new bipartisan spirit in the Senate and Congress.

Pfizer Pfizer is prioritizing its research and development efforts in areas with the greatest scientific and commercial promise: immunology and inflammation, oncology, cardiovascular and metabolic diseases, neuroscience and pain, and vaccines. Through major research efforts across multiple modalities—including small molecules, biologics and vaccines—Pfizer is developing the medical solutions that will matter most to the people we serve. Planned specialized efforts in biosimilars as well as orphan and genetic diseases also illustrate our dedication to develop and deliver innovative medicines and vaccines that will benefit patients around the world.


Pittsburgh Life Sciences Greenhouse The Pittsburgh Life Sciences Greenhouse (PLSG) provides capital investments and customized company formation and business growth services to our region’s life sciences enterprises. We support biosciences companies with promising innovations in the following concentrations: Biotechnology Tools, Diagnostics, Healthcare IT, Medical Devices and Therapeutics.

Protea Biosciences Protea is a leader in the emerging field of Bioanalytics. The company develops new technology for the identification, characterization, and quantitation of biologically-important molecules for basic research, pharmaceutical development, and diagnostic applications. We develop new bioanalytical methods that enable the direct analysis of proteins and other biomolecules, and apply our capabilities to support your laboratory’s research objectives.

Steptoe & Johnson Steptoe & Johnson is a business-focused firm with particular strengths in energy, labor, employment, and litigation. For nearly a century, Steptoe & Johnson has demonstrated its commitment to quality, devoting personal attention, experience, skill, and resources to client-focused service.

TechConnect TechConnectWV is a coalition of professionals dedicated to growing and diversifying West Virginia’s economy by advancing technology-based economic development (TBED). We serve mainly as facilitators, enhancing awareness, increasing collaboration, and raising the discussion of important issues to spur technology development and commercialization in West Virginia.

Vandalia Research Vandalia Research is a biotechnology company located in Huntington, WV. Vandalia’s core technology is its Triathlon DNA production technology, which allows for the large-scale production of specific DNA sequences using the polymerase chain reaction (PCR). Vandalia Research is actively developing applications related to linear expression cassettes (LECs) produced by the Triathlon system.


WV Development Office The West Virginia Development Office’s role is to: support existing businesses in the expansion and retention of their operations; attract new businesses to locate in the state; generate new leads and prospects of potential investors; diversify West Virginia’s economy by marketing to targeted industries; provide comprehensive development assistance; provide research, planning and technical support; work with local governments and economic developers to improve the quality of life in communities; and market West Virginia at primary and secondary target industry trade shows.

WV Higher Education Policy Commission The West Virginia Higher Education Policy Commission is responsible for developing, establishing, and overseeing the implementation of a public policy agenda for the state’s four-year colleges and universities. It is charged with oversight of higher education institutions to ensure they are accomplishing their missions and implementing the provisions set by state statute.

WV Small Business Development Center The West Virginia Small Business Development Center (WVSBDC) is a division of the West Virginia Development Office and is funded by the State of West Virginia, the United States Small Business Administration, and our Community Colleges and Workforce Investment Board Partners. The WVSBDC promotes economic development through a program of practical, interrelated services, providing assistance to existing small businesses and the emerging entrepreneur. The WVSBDC provides many free services that assist you, the entrepreneur, in your business venture.


The following companies expressed interest, but regrettably were unable to attend: Apple; Bayer MaterialScience; Biometric Center of Excellence; BIOWV; Boekel Scientific; Cognition Therapeutics; Fox Chase Chemical Diversity Center, Inc.; Genus Oncology, LLC; GlycoMimetics, Inc.; Good Measures; I-79 Development Council; Integrated Management Services, Inc.; ISTA Pharmaceuticals; Jackson Kelly PLLC; Joint Research and Development; Kinexum, LLC; Knopp Neurosciences; M’s Science Corporation; MATRIC; Merck; Meritus Ventures; Mnemosyne Pharmaceuticals; Monsanto Corporation; Mountain Capital; Norgenix Pharmaceuticals; Onconova; PhaseBio; Precision Therapeutics; Progenesis Technologies, LLC; Red House Consulting; Saint-Gobain Crystals; Small Business Administration; SensL; Siemens Healthcare; Southwest Michigan First Life Science Fund; Spectragenetics; Supernus Pharmaceuticals; TSI, Inc.; Tetra Discovery; Toshiba Imaging; TRAX Biodiscovery; Trevena; Vertex Pharmaceuticals; WV Angel Network; and Zoll Medical Corporation.


FACULTY RESEARCH Computational Drug Surveillance The focus of this work is to develop novel computational methods for assisting in efficient and effective post-marketing surveillance of approved drugs. The work includes computational methods for analysis and detection of potential drug safety problems, such as drug-drug interactions, and adverse drug events. This line of research is of interest to federal regulatory agencies, such as the US Food and Drug Administration (FDA), pharmaceutical companies, hospitals, and the general public. Donald Adjeroh Computer Science and Electrical Engineering 304-293-9681

Reducing Muscle Wasting in Aging Our laboratory studies muscle loss due to aging and disuse in heart and skeletal muscle and seeks to find strategies to prevent or reduce this muscle wasting. Loss of skeletal muscle with aging is called sarcopenia, which is characterized as an age-associated loss of muscle fiber size and muscle fiber number with aging. This loss of muscle tissue results in decreased muscle strength and a loss of independence, and this is a major problem in the elderly population. Our laboratory uses models of hypertrophy and atrophy to study the neural dependent and neural independent cellular and molecular mechanisms leading muscle growth and muscle atrophy in aging muscles. We are particularly interested in the process of apoptosis that occurs in muscle stem cells (satellite cells) and myonuclei and identifying strategies to reduce apoptotic signaling and improving muscle growth and regeneration in aging and diabetes. Stephen E. Alway Exercise Physiology 304-293-0772


Genomic Biomarkers of Stroke and Cardiovascular Disease My program of research is centered on the use of genomic methodologies to identify biomarkers of cardiovascular disease, understand their relationship with post-stroke inflammation and immunity, and define the contributing environmental factors related to health disparity in underserved populations. Our research portfolio includes projects that begin with the patient at the bedside and aim to bridge the gaps between the bench and implementation in the community. Many of our projects go through a rigorous process of inquiry to ensure potential for commercialization and clinical practice change. Taura L. Barr School of Nursing and Emergency Medicine 304-293-0501

Biomedical Imaging: Segmentation and Tracking of Human Cells Capability to detect, segment, and track (human) cells using computer vision algorithms applied on image sequences (video). Thirimachos Bourlai Computer Science and Electrical Engineering 304-293-4326

Immune Mechanisms Associated with Resistance to Helminth Parasitism The research in my lab uses a breed of sheep that can effectively clear a parasite infection without developing adaptive immunity. Our research focuses on innate immune mechanisms that are involved in this parasite expulsion and how we may better modulate immune responses in sheep not resistant to parasite infection. Scott Bowdridge Animal and Nutritional Sciences 304-293-2003


Early Signal Transduction Responses for Prediction of Viability My laboratory primarily focuses on the phosphorylation/dephosphorylation cycle, mediated by kinases and phosphatases, which is responsible for the coordination of many cellular responses to xenobiotics. This simple cycle is embedded in networks that use positive and negative feedback to generate extremely diverse functions, including apoptosis. To this end, we have developed a suite of approaches to predict 24 hour viability of early stage cancer therapeutics at very early time points post-dose (< 1 hour), by simply interpreting the dynamic activity of signal transduction networks in response to xenobiotics. Jonathan Boyd Chemistry 304-615-9627

3D Body Scanner Provides Athletic Apparel Designs to Increase Physical Activity Among Obese Adults With the use of a 3D body scanner, we are able to acquire 3D plots of the human figure. Doing this with obese individuals we are able to create clothing that conforms to larger shapes and sizes that are not readily available in standard retail environments. Standard retail environments offer limited sizes in athletic apparel and this discourages obese individuals from engaging in physical activity. Previous studies confirm that physical activity increases when obese adult women wear athletic clothing that is specifically designed for their figure. New plus-size athletic apparel designs are in the process of being created through a new technique. Deborah Christel Fashion Design and Merchandising 304-293-3425

Investigating the Inter-relationships among Childhood Asthma, Obesity, and Metabolic Disease The WVU Department of Pediatrics offers a series of active research studies examining childhood asthma, obesity, and metabolic disease both directly and in terms of how these conditions share similar physiological, psychoso12

cial, cognitive, and contextual factors. Each of these conditions significantly threatens children's health and have risen simultaneously over the past decade. This multi-faceted and interdisciplinary project examines several facets of these conditions with the goal of understanding their shared (and diverse) origins and trajectories. Lesley Cottrell Pediatrics 304-293-1149

Wood-based Biomaterials My research involves fundamental studies on biomaterials, specifically composites, from wood and combination of wood and non-wood materials. Also, I have been developing biofilms with cellulose and biochar nano-particle addition for improving physical and mechanical properties. My research related to biomaterials focuses on development of biodegradable products and investigation of bonding interactions between dissimilar raw materials. My research also looks at non-destructive evaluation of biomaterials using optical and ultrasonic testing techniques. David DeVallance Forestry and Natural Resources 304-293-0029

Bionanotechnology for Biomedical Engineering Professor Dinuâ&#x20AC;&#x2122;s research spans over a wide range of disciplines, from molecular biology to materials science and from engineering to fundamental science. Specific focuses are on: (1) studying drug uptake, kinetics and metabolism in real time, (2) mechanisms responsible for nanomaterial-cellular uptake, transport and nanomaterial-induced toxicity; (3) designing enzymebased nanomaterial's with extraordinarily high activity and stability to be used for decontamination of chemical and biological toxins; and (4) the application of cellular motors for molecular nanomanufacturing. Cerasela Zoica Dinu Chemical Engineering 304-293-9338


Computer Vision, Image and Video Analysis, Medical Image Analysis The WVU Vision Laboratory,, is focusing on core aspects of Computer Vision, Image and Video analysis research. Those include: (a) object detection, classification and segmentation; (b) scene analysis and human behavior analysis; (c) social network analysis from video; (d) multispectral analysis of video; (e) analysis of 3D data acquired at video rate; (f) data visualization and virtual environments. Typical Bioscience and Biomedical applications include computer aided detection (CADe / CADx) in medical images (CT scans and MRI), tracking in ultrasound video sequences, cell behavior analysis in electron microscopy, 3D visualization of neuronal tissues, and low-cost motor analysis of patients for rehabilitation monitoring. Gianfranco Doretto Computer Science and Electrical Engineering 304-293-9133

Real-time, Rapid-cycle Performance Improvement Evaluating patient care is crucial to its improvement. Typically, performance improvement in hospitals uses a top-down, centralized approach designed to safeguard the organization from risk, rather than involving patients and clinicians at the unit level, inadequately addressing quality and wasting enormous resources. Mobile devices offer a patient-centered and feasible solution for rapid, actionable practice changes at the unit level. Joanne R. Duffy Nursing 304-293-0761

Prevention of Disease Recurrence following Breast Cancer Chemotherapy Recurrence of tumors years after chemotherapy is the major cause of mortality in breast cancer. Chemoresistant cancer stem cells are responsible for recurrence. We have developed a method to prevent cancer stem cells from emerging during and after this therapy. Our method will greatly improve patient survival and quality of life. Steven M. Frisch Biochemistry and Cancer Center 304-293-2980


Nanoparticles for Cancer Detection and Treatment Nanoparticles have proven to be an efficient means of delivery for drugs. Magnetic nanoparticles are currently used as a MRI contrast agent. By combining these two modes, multifunctional nanoparticles can be created that can be used for both detection and treatment of disease. Our current research is directed at optimizing the core material of the nanoparticle and adding disease (cancer) specific targeting elements to improve the imaging characteristics and selectivity of the nanoparticles. Peter Gannett Basic Pharmaceutical Sciences 304-293-1480

Improving Diagnosis and Recovery after Neurological Injury Millions of people in the United States live with movement disability. Our research tests the potential of modern technology to transform diagnosis and rehabilitation of movement impairment. The goal of our research is to provide patients with a more affordable, engaging, and accessible form of rehabilitation using a home-based mobile medicine approach that will empower the patient to take charge of his/her recovery. Another goal of our research is to enable doctors and other medical professionals to objectively assess movement impairment, develop a personalized evidence-based treatment plan, and to administer therapy and to track patient progress remotely. Such home-based rehabilitation system will provide long-term personalized care that is not only cost effective, but also accessible to those living in remote locations. Valeriya Gritsenko Human Performance 304-293-7719

Biometrics, Computer Vision, and Multimedia Dr. Guodong Guoâ&#x20AC;&#x2122;s research areas include: Computer Vision, Image and Video Computing, and Biometrics. To be specific, his research includes: (1) Human identification and human description based on computational analysis


of face and body images, using different modalities, for security, law enforcement, and surveillance; (2) Human age estimation via face images; (3) Human gender and ethnicity classification in face images; (4) Human facial expression or emotion analysis and recognition; (5) Facial makeup analysis and digital anti-aging; (6) Human action/behavior recognition from videos for intelligent video surveillance; and (7) Social computing and social network analysis using Computer Vision and Machine Learning techniques. Guodong Guo Computer Science and Electrical Engineering 304-293-9143

Identifying Novel Biomarkers for Diagnosis, Prognosis and Medical Surveillance Our research utilizes whole genome assays to identify novel gene markers for diagnosis and prognosis of human lung cancer and breast cancer. These genomic biomarkers could detect or predict disease onset and outcome before cancer symptom is morphologically detectable. We are also developing highthroughput assays for screening toxic nanoparticles for manufacturing safe nanomaterial's for humans and workforce medical surveillance. Nancy Lan Guo School of Public Health and Cancer Center 304-293-6455

Thermally Responsive Nanogels for Microscale Sample Processing Microscale separations are integrated with multi-step sample processing such as biopolymer digestion, analyte concentration, sample fractionation, and separation-based sorting. Traditionally system integration is accomplished through sophisticated design of modular microfluidic devices that perform multiple processing functions. These impressive devices are highly developed and dedicated for a specific application. Research in advancing the use of smart materials nanomaterials provides an innovative alternative that


supports flexible and reprogrammable sample processing as well as tunable microscale bioseparations. Lisa Holland Chemistry 304-293-0174

First Row Transition Metal Complexes for new C-C Bond Forming Reactions We are interested in developing new C-C bond forming reactions with biological significance. One goal is to develop a trifluoromethylation method employing trifluoroacetic acid (CF3CO2H) as an inexpensive source of CF3. Because the incorporation of CF3 can greatly influence the physical properties of a compound, trifloromethylated derivatives are attractive targets for the pharmaceutical and agrochemical industries. Also of interest is the development of new first row transition metal complexes bearing neutral thione ligands that serve as model compounds for enzymes such as vanadium nitrogenase. Jessica Hoover Chemistry 304-293-0367

Bioanalytical and Forensic Mass Spectrometry Our group focuses on instrumentation development of mass spectrometers for biomedical and forensic applications. We are currently developing new instruments and new methods of determining the sequence and conformations of biological ions such as peptides, lipids, carbohydrates and nucleic acids. In separate work, we are developing methods for the direct analysis of drugs and drug metabolites in biological matrices such as hair. Glen Jackson Forensic and Investigative Science 304-293-9236


Genetic Variations within the Defensing Gene Cluster and the Relationship to Oral Mucosal Barrier Function and Viral Tropism in HIV/AIDS The antimicrobial peptides hBD-2 and 3 encoded by DEFB4 and DEFB103A respectively are expressed in differentiating epithelia and are induced during infections and inflammation, and reach biologically relevant concentrations in the systemic circulation. The β defensing gene cluster on 8p23.1 is highly polymorphic and the genes DEFB4 and 103A vary en bloc with copy number ranging from 2-12 per diploid genone. Copy number variation varies with transcript level and serum concentration, and thus may influence HIV disease progression. The genes DEFB4/103A encoding β defensing 2 and 3 respectively, inhibit CXCR4-tropic (X4) viruses in vitro. We identified that DEFB copy number (CNV) influences time to AIDS in a disease progression group specific manner (slow versus fast progressors) in the Multicenter AIDS Cohort Study (MACS). Our current findings may contribute to the current understanding of how genetic predisposition influences AIDS progression. Richard Jurevic Oral Diagnosis 304-293-1142

Model-based Inference of Causal Influence Networks in Uncertain Systems Biological systems exhibit intrinsic uncertainty; yet, understanding how the basic unit in these systems (i.e., cells) processes information and understanding how heterogeneous populations of cells collectively regulate tissue homeostasis are central questions in the medical and biotechnology fields. To address these questions, we use a combination of high content experimental and computational methods to infer how cells process information and to identify novel mechanisms of cellular cross-talk. Our particular emphasis is on how cancer cells manipulate host immunity; yet, the methods are generalizable to many different fields that exhibit intrinsic uncertainty. Three relevant publications: 1. Klinke DJ. An empirical Bayesian approach for modelbased inference of cellular signaling networks. BMC Bioinformatics 10:371 (2009). 2. Klinke DJ, Cheng N, Chambers E. Quantifying crosstalk among interferon-γ, interleukin-12, and tumor necrosis factor signaling pathways within a TH1 cell model. Sci Signal 5:ra32 (2012). 3. Kulkarni YM, Chambers E, McGray AJ, Ware JS, Bramson JL, Klinke DJ. A quantitative systems approach to identi-


fy paracrine mechanisms that locally suppress immune response to Interleukin-12 in the B16 melanoma model. Integr Biol (Camb) 4:925--936 (2012). David Klinke Chemical Engineering 304-293-9346

Endodontic Research Dr. Komabayashi is well versed in the areas of pulp capping therapy, and nanomaterial and adhesive material for endodontics. His research focuses on different materials used for capping teeth, as well as ways to make such procedures cost effective and time efficient. Takashi Komabayashi Endodontics 304-293-3321

Adolescent Caffeine Research in West Virginia Caffeine use among adolescents has increased greatly in recent years, for example through consumption of energy drinks. The consequences of such use are not well understood, which form the objective of this research program. The aims are to: 1) construct a measure instrument on adolescent caffeine use in West Virginia; 2) measure the prevalence of caffeine use among adolescents in the state; and 3) identify key predictors to adolescent caffeine use while controlling for important covariates such as demographics, SES factors, and sugar and guarana intake. Alfgeir L. Kristjansson School of Public Health 304-293-3129

The Impact of Surfaces on Protein Aggregation associated with Neurodegenerative Diseases There is a vast number of neurodegenerative diseases, including Alzheimer’s disease (AD), Parkinson’s disease (PD), and Huntington’s disease (HD), associated with the rearrangement of specific proteins into non-native confor-


mations, which promote aggregation and deposition within tissues and/or cellular compartments. A detailed understanding of the influence of (sub) cellular surfaces in driving protein aggregation and/or stabilizing specific aggregate forms could provide new insights into toxic mechanisms associated with these diseases. Using a variety of scanning probe microscopic techniques, the interaction of the β-amyloid (Aβ) peptide associated with AD and mutant huntingtin (htt) proteins associated with HD with liquid/solid interfaces, and in particular lipid membranes, are being explored. Justin Legleiter Chemistry 304-293-0175

Nanomedicine for Preventing and Treating Bone Infections Chronic and recurrent infections occur frequently, and more strains of bacteria are becoming antibiotic resistance. Our research focuses on tuning appropriate immune responses against infections and developing nanotechnology approaches to reduce chronic and recurrent infections. We also develop nanotechnology drug delivery strategies to improve wound healing and to treat cancers. Bingyun Li Orthopaedics 304-293-1075

Collagen-like Genes and Proteins as Detection and Therapeutic Targets of Infectious Agents The current research of the laboratory is focused on (i) microbial collagens: structure-function relationship, (ii) microbial biofilms, and (iii) immune evasion of bacterial pathogens. Additional projects include pathogen detection based on sequence polymorphisms in collagen-like genes in association with WVNanoSAFE Program. Slawomir Lukomski Microbiology, Immunology & Cell Biology 304-293-6405


Sit_Alone PET Brain Imager for Neurological Diseases We are developing a dedicated compact mobile brain/head/neck PET imager. Variants of the general concept are: (1) implementation as a PET insert in any clinical MRI to provide powerful and low cost PET/MRI brain imaging, (2) low dose brain PET imager, and/or (3) high resolution PET brain imager. One of the implementations is a stand-alone (Sit_Alone) imager providing opportunity to image patients in comfortable upright position and also in brain research. Low dose and high resolution variants are being developed. Low dose mode of operation can permit application in screening and/or longitudinal studies during therapy in many neurological diseases: dementia, stroke, mental diseases, and in brain cancer. Stan Majewski Radiology 304-293-1878

Mobile Health (mHealth) applications in Chronic Illness in Rural Healthcare Settings Mobile health (mHealth) is an emerging field that has been defined as â&#x20AC;&#x153;medical and public health practice supported by mobile devices, such as mobile phones, patient monitoring devices, personal digital assistants, and other wireless devicesâ&#x20AC;? (World Health Organization, 2011). Used as an integrated tool, mHealth may improve the ability of healthcare providers in rural areas to provide care, improve access to care for underserved populations, and improve biophysical outcomes of care for persons with diabetes in rural, underserved populations. Our objective in this presentation is to present the potential impact of mHealth interventions for community dwelling individuals with chronic illnesses and present the potential for collaboration with leaders outside the University setting. When the proposed development of the electronic infrastructure has been complete, it is our expectation that mHealth technologies will be integrated into existing rural health clinics. Thus, allowing for the first time, the implementation of a novel and much-needed approach to rural healthcare delivery. Jennifer Mallow Nursing 304-293-1402


MicroRNA Signatures of Tumor-derived Exosomes as Diagnostic Biomarkers The objective of this project is to develop a diagnostic and prognostic tool (expression microarray) based on the detection of small non-coding RNAs known as microRNAs (miRNAs) in the blood stream of cancer patients. Several types of cancers are diagnosed at advance stage and consequently with a poor survival rate. Recent discoveries showed that miRNAs are critical in the development of cancer. Interestingly, some tumors are able to release miRNAs to the blood stream in small membrane vesicles known as exosomes. We believe that the development of a miRNA profiling microarray of circulating tumor exosomes could be an important tool for screening people with inherited predisposition to develop cancer and also to follow tumor behavior. Ivan Martinez Microbiology and Cancer Center 304-581-1934

Medications for Neurological and Psychiatric Disorders Our lab uses a multidisciplinary approach to develop potential new therapeutic agents for the treatment of neurological and psychiatric disorders. In vitro and in vivo approaches are used. Current collaborators include medicinal chemists and clinicians. Rae R. Matsumoto Basic Pharmaceutical Sciences 304-293-1450

Relationship between Fatty Acid Metabolism, Inflammation, and Insulin Sensitivity Our lab is currently employing pharmacological approaches to modify intracellular fatty acid metabolism to minimize inflammation and increase insulin sensitivity. Additionally, we are employing metabolomics technologies to profile the mammalian metabolome in the absence or presence of these metabolic modifiers. Research is aimed at minimizing insulin resistance and lipolysis in obese cows and monogastric models. Joseph W. McFadden Animal and Nutritional Sciences 304-293-1883


Computational Biophysics of Membrane Proteins G protein-coupled receptors (GPCRs) are a family of membrane proteins ubiquitous in cellular signaling processes, but their activation mechanisms are difficult to elucidate. X-ray crystallographic structures of several types of GPCRs have begun to reveal important aspects of conformational changes in the activation process. However, the dynamics of this process cannot be completely explained with static structures. Our lab uses molecular dynamics (MD) simulations to understand the equilibrium behavior of GPCRs in native-like cell membrane environments, with the aim providing an atomistic view of GPCR activation. These studies are directly related to addressing a wide range of biomedical issues such as visual disorders, obesity, and cancer. Blake Mertz Chemistry 304-293-9166

Sleep and Sleep Disorders The Sleep and Sleep Disorders research laboratory focuses on predictors and consequences of pediatric and postpartum sleep disturbance. Methodological innovations are also a primary focusâ&#x20AC;&#x201D;particularly development and validation of novel approaches to non-intrusive human physiologic data collection. Hawley Montgomery-Downs Psychology and Behavioral Neuroscience 304-293-1761

Nonlinear Characterization of Breast Cancer using Multi-Compression 3D Ultrasound Elastography in Vivo The main objective of this article is to introduce a new nonlinear elastography based classification method for human breast masses. Multicompression elastography imaging is elucidated in this study to differentiate malignant from benign lesions, based on their nonlinear mechanical behavior under compression. Three classification parameters were used and compared in this work: a new nonlinear parameter based on a power-law behavior of the strain difference between breast masses and healthy tissues, mass-


soft tissue strain ratio and the mass relative volume between B-mode and elastography imaging. Using 3D elastography, these parameters were tested in vivo. A pilot study on ten patients was performed, and results were compared with biopsy diagnosis as a gold standard. Initial elastography results showed a good agreement with biopsy outcomes. Sam Mukdadi Mechanical and Aerospace Engineering 304-293-3110

Musculoskeletal Injury Prevention Dr. Ning's research interests lie in the area of industrial ergonomics, and musculoskeletal injury prevention. His previous research investigated the occupational risk factors that contribute to work-related injury. Most recently his work has been focusing on the investigation of basic biomechanical functions of low back musculature and the development of a new work-rest scheduling model. Xiaopeng Ning Industrial and Management Systems Engineering 304-293-9474

Engineered Nanomaterial Safety Assessments for Cardiovascular Applications Our research program merges three distinct disciplines: nanotechnology, microvascular physiology, and toxicology. The purpose of our endeavors is to identify safe engineered nanomaterials for use in a variety of biomedical applications that will benefit human health. Examples include drug delivery platforms, diagnostic imaging and implantable devices. We study the microcirculation because most every disease and growth state originates from this critical level of the cardiovascular system. Animal models of toxicity are used to determine the conditions and limits of nanomaterial exposure; as well as characterize the mechanisms of interactions with host tissues that minimize


human risk while capitalizing on the unique physical and chemical properties of materials at the nano-scale. Timothy R. Nurkiewicz Physiology and Pharmacology 304-293-7328

Biological Survey Using Unmanned Aerial Vehicles Over the last seven years, researchers from Entomology and Aerospace Engineering at West Virginia University have developed small, low-cost, and flexible unmanned aerial vehicles (UAV) for surveying organisms including insects, plants, and plant diseases from the sky. The versatile UAV has ability to accomplish numerous jobs including environmental monitoring, detecting pests in agricultural systems, and sampling organisms in the air. Recently, the team has developed a UAV system that can precisely deliver natural enemies of insect pests from the air. Yong-Lak Park Entomology 304-293-2882

Decellularized Stem Cell Matrix; a Novel Cell Expansion Substrate for Cartilage Engineering and Regeneration Cell-based therapy is a promising biological approach for the treatment of cartilage defects. Due to the small size of autologous cartilage samples available for cell transplantation in patients, chondrocytes need to be expanded to yield sufficient cell number for cartilage repair. However, either chondrocytes or adult stem cells tend to become replicatively senescent once they are expanded on conventional plastic flasks. Many studies demonstrate that the loss of cell properties is concomitant with the decreased cell proliferation capacity. This is a significant challenge for cartilage tissue engineering and regeneration. Despite much progress having been made in cell expansion, there are still concerns over expanded cell size and quality for cell transplantation application. Recently, in vivo investigations in stem cell niches have suggested the importance of developing an in vitro stem cell microenvironment for cell expansion and tissue-specific differentiation. Our and other


investigatorsâ&#x20AC;&#x2122; work indicates that a decellularized stem cell matrix may provide such an expansion system to yield large-quantity and high-quality cells for cartilage tissue engineering and regeneration. Further investigations of the underlying mechanism and in vivo regeneration capacity will allow this approach to be used in clinics in the near future. Ming Pei Orthopaedics 304-293-1072

New Strategies in Bioorganometallic Chemistry The Popp research group is interested in expanding the utility of transition metals in biological settings. Our efforts are centered in two areas: 1) incorporating novel metal binding domains in synthetic peptides or proteins and 2) developing new transition-metal-catalyzed approaches to manipulate specific functionalities on peptides or proteins. Brian Popp Chemistry 304-293-0773

WVU Shared Research Facilities WVU Shared Research Facilities (WVU SRF) provides researchers with the opportunity to use cutting-edge materials, computational modeling, high performance computing equipment and science and engineering instrumentation. WVU SRF are open to all, including researchers at government laboratories and industries. Our staff provide training, guidance, and assistance in operating the instruments and perform routine maintenance. Among our labs, we have Cleanroom facilities, Electron Microscopy facilities, High Performance Computing facilities, Bio-Nano Research facilities and Materials Fabrication and Characterization facilities. Website: Lisa Sharpe, Don McLaughlin, Siera Talbott, Marcela Redigolo, Kolin Brown, Weiqiang Ding, and Harley Hart WVU Shared Research Facilities 304-293-6872


Healthcare Workforce Development: Access in Secure Settings Prison administrators have a multitude of challenging management, budgetary, and personnel issues. One of the most challenging personnel issues is the maintenance of a highly trained workforce with a low turnover rate. We addressed these seemingly intractable problems with a revolutionary training and development program, developed and funded with over $1.2 million dollars by the Health Resources and Services Administration. This system utilizes non-threatening educational strategies such as web-based learning environments, simulation of high-risk situations, opportunities to practice and debrief, and peer mentoring to help promote a level of skill acquisition and self-efficacy through knowledge of the correctional environment and the corrections population. The development team is currently seeking partners to help expand the capacity of and replicate the mobile prison environment simulators and assist in the development of an education and data management network that meets the requirements of correctional systems security codes. Deborah Shelton Nursing 304-293-1903

Regulators of Heterotrimeric G Protein Signaling as Novel Drug Discovery Targets Our groupâ&#x20AC;&#x2122;s long-standing research focus is in identifying novel regulators of heterotrimeric G protein signaling, having discovered the RGS family of Ga subunit GTPase-accelerating proteins (GAPs) in 1996 and the GoLoco motif protein family of Ga subunit scaffolds in 1999. We recently engineered three different mouse strains, each deficient in a particular RGS or GoLoco motif protein, that highlight the roles each of these proteins can play in selective pathophysiological conditions. One RGS-deficient mouse strain exhibits compulsive self-grooming and anxiety behaviors reminiscent of human obsessivecompulsive spectrum disorders. Mice deficient in GPSM3, a GoLoco motif protein restricted to monocytes, are protected from collagen antibodyinduced arthritis (recently published as Giguère et al. 2013 Mol. Immunology). Finally, we have identified RGS21 as selectively expressed in taste buds and putative tastant-responsive airway epithelia (recently published as Cohen


et al. 2012 J. Biol. Chem); a newly-created, conditional RGS21 knockout mouse strain is likely to reveal the role of RGS21 in responses to bitter and sweet compounds on the tongue and xenobiotics inhaled in the lung. All three G protein regulators have the potential to be targeted by small molecule modulators to ameliorate human pathophysiological conditions. David Siderovski Physiology and Pharmacology 304-293-4991

Targeting Mitochondria for Brain Protection in Stroke My laboratory conducts drug discovery studies aimed at the identification of drug targets and potential new drugs for the protection of the brain from neurodegenerative disease and acute neuron compromising conditions, like stroke and traumatic brain injury (TBI). To date, we have discovered two classes of compounds that protect neurons by targeting mitochondrial structure and function. These compounds enhance respiratory capacity of the mitochondria and preserve mitochondrial structure. This ongoing effort has resulted in clinical drug trials in Alzheimerâ&#x20AC;&#x2122;s disease and TBI and pre-clinical development for Parkinson's disease. James Simpkins Physiology and Pharmacology 304-293-7430

Targeted Therapeutics for Specific Prostate Cancers Molecular recognition elements are biomolecules (DNA, RNA, peptide, antibody) that bind tightly and specifically to a target of interest. Antibody singlechain variable fragments (scFv) have been isolated which bind preferentially to a cancerous or benign prostate cell line, but not to other cells. These scFvâ&#x20AC;&#x2122;s may be applied in targeted drug delivery and sensitive detection applications. Letha Sooter Basic Pharmaceutical Sciences 304-293-9218


Low-cost Mobile Dedicated PET Instrumentation Nuclear medicine techniques have firmly established themselves as an invaluable tool in many areas of healthcare. Further developments in such techniques are often hampered by high costs of necessary instrumentation. Our research aims to significantly reduce the cost of nuclear medicine examinations by developing and implementing dedicated, mobile, hand-held PET detectors and systems. Such systems could be used for diagnostic, intraoperative, treatment monitoring applications. Flexible geometries and close proximity of the detectors to the scanned regions will allow for higher detection sensitivities, thus reducing the radiation doses to the patients. Alexander Stolin Radiology 304-293-6929

Bacterial Rapid Detection Using Optical Scattering Technology Initially designed for bioterrorism and rapid detection of unusual food borne pathogens, we utilized the BARDOT system to establish a library of wound isolate scatter patterns, then evaluated its ability to rapidly triage wound isolates by patient site of infection, hospital unit, mechanism of resistance (MoR) and WV origin, simultaneously; under 2 minutes/culture plate with mixed microbiology, independent of Gram Stain or species the accuracy was greater than 90% compared to 48 -72 hour traditional culture procedures. John G. Thomas Pathology 304-293-3204

Utilizing the WV Airway Disease Simulator to Evaluate Cather Removal of Luminal Microbial-Accretion Occlusion Ventilator Assoc Complex (VAC) is now the most expensive ICU consequence of mechanical ventilation ($40,000/case), exacerbated by endotrach occlusion and prolonged weaning, effectively reducing the ETT size from 8 to 7 in less than 24 hours. We evaluated 2 new suction/wiper catheters in the Simulator utilizing a 2 bacterial species and Candida albicans complex biofilm over


14 days, and found significant reduction in accretion occlusion, organism bioburden, but not the adherent Stage III biofilm, suggesting repeat clearing would be necessary. John G. Thomas Pathology 304-293-3204

Feeding Female Rats Different Sources of Omega-3 Polyunsaturated Fatty Acids Alters Lung Fatty Acid Composition, Eicosanoid Metabolism and Antioxidant/Oxidant Balance The effects of n-3 PUFA intake on fatty acid composition and in turn, potential effects on inflammation and oxidative/antioxidant balance in the lungs is important because the general population is being encouraged to consume more n-3 PUFAs. The objectives of this study were to determine whether providing rats different sources of n-3 PUFAs affects lung fatty acid composition, eicosanoid metabolism, and oxidative stability. Rats were randomly assigned to be fed corn oil (CO) or popular n-3 PUFA supplements of flaxseed (FO), krill (KO), menhaden (MO), salmon (SO) or tuna (TO) oils. Fatty acid profile measured by gas chromatography showed lung fatty acid composition resembled the dietary oil consumed. The n-3 PUFA, alpha-linolenic acid (ALA, 18:3n-3) was only detectable in the lungs of rats fed ALA-rich FO. Rats fed FO, but not the marine oils, up-regulated (P=0.02) 5-lipoxygenase (5-LOX) gene expression, as determined by RT-qPCR and reduced (P=0.02) proinflammatory leukotriene B4, as determined by enzyme immunoassay. There was no significant difference in lung oxidative stability among the diet groups. Of the n-3 PUFA sources, FO increased tissue ALA content without increasing lipid oxidation and influenced leukotriene synthesis by regulating genes in the eicosanoid signaling cascade. The study results have implications for individuals consuming n-3 PUFA supplements to promote health and for the development of recommendations regarding lung health. Janet Tou Animal and Nutritional Sciences 304-293-1919


Theory of Nonlinear Partial Differential Equations (PDE) My research interests lie in the general area of the theory of nonlinear PDE and specifically in three distinct areas: the theory of hyperbolic systems of conservation laws; kinetic theory in continuum modeling; and diffusiondispersion equations and Strichartz estimates. I work theoretically on applied models. Charis Tsikkou Mathematics 304-293-6046

Combined IMS-HDX-MS for Characterization of Macromolecular Complex Structure New instrumentation is currently being developed that combines ion mobility spectrometry with hydrogen-deuterium exchange techniques and mass spectrometric detection. The novel approach will be applied to the characterization of macromolecular complex structure (e.g., protein complexes). The determination of structures for many of these species have not been determined because of their size and transitory nature. The new approach has the potential for important discoveries related to these cellular machines and may, therefore, yield important information about disease progression mechanisms. Stephen J. Valentine Chemistry 304-293-4937

Reversal of Preeclampsia, Lung Hypertension, Asthma and Exercise Induced Asthma We found that the above diseases are linked to insufficient availability of nitric oxide. By supplying nitric oxide precursors and certain key antioxidants in a continuous manner, these diseases can be treated with a favorable outcome. Although some of these diseases can be partially treated with drugs, our supplements give more complete results with little to no side effects at lower


costs. This combinational treatment basically relieves oxidative stresses and the body cures itself. Knox Van Dyke Biochemistry 304-293-5449

Biomass Utilization for Bioproducts and Biofuels Biomass for bioenergy has been extensively studied at West Virginia University through several projects funded by USDA and USDOE. Our research focuses on feedstock development, conversion technologies, product analysis and commercialization. Our research partners include government agencies, regional industries and other universities. Jingxin Wang Forestry and Natural Resources 304-293-7601

Plant Biotechnology to Increase Drought and/or Heat Tolerance Climate change due to global warming has been one of the most debated topics and has already impacted our environment and lives. Current climate change has resulted in an unprecedented amount of climate variability and damage to our environment and habitat. The most severe long term damage from this change would be the shortage of food sources due to drought and/ heat stresses. The long term goal of our research is to increase the quality of crops by enhancing drought and/or heat tolerance. Specific aims are to 1) document response to drought/heat stresses among weeds tolerant to those stresses and economically important Solanaceae plants, 2) identify genes that are responsible for drought/heat stresses, 3) genetically engineer crops with increased tolerance to drought/heat stresses, and 4) evaluate the effects of engineered plants on environment. Nicole Waterland and Youyoun Moon Plant and Soil Sciences 304-293-2969


Use of a Unique Endotracheal Tube Clearing Device to Decrease Airway Resistance The formation and adherence of biofilm secretions within the ETT lumen has been perceived to impact airway resistance, resulting in prolonged MV and potential VAP development. Our primary objective was to evaluate the endOclear endotracheal tube clearing device (ECCD) and its ability to decrease ETT occlusion and return â&#x20AC;&#x153;nominal functionalityâ&#x20AC;?. ETTs obtained from 40 ICU patients at WVUH were evaluated using a modified multi-station airway resistance simulator at three flow rates, 30, 60, 90 L/min. Pre and Post ECCD clearing values were recorded and compared with size-matched controls. Following ETT clearing, all ETTs were returned to within 1-13% of control values, independent of occlusion location, tube size or LOI. Routine use of ECCD appears to improve airway function in patient ETTs, potentially reducing LOI and impacting weaning trials. Christopher Waters Pathology 304-685-8957

A High-specificity Test for Diagnosis/Prognosis of Colorectal Cancer Reliable diagnosis and prognosis are important for prevention and treatment of colorectal cancer (CRC), one of the deadliest diseases in developed countries. Recently, several EphB family proteins have been reported to be promising indicators for CRC progression. Our studies also suggest that EphBs play important roles in regulating cell signaling that is known to cause CRC. Here we propose to develop a highly sensitive and specific biochemical test to determine EphB levels, which can be used for diagnosis and prognosis of CRC. Shuo Wei Biology 304-293-2106


Restoration of Sensorimotor Functions with Neuroprosthetics The goal of my research program is to develop a reliable peripheral interface capable of controlling dextrous prosthetic devices. Specifically, I develop neuromechanical models for real-time online processing and to lead the development of optimization algorithms for improving the performance of model and prosthesis control and their adaptation after stroke and spinal cord injury. Sergiy Yakovenko Exercise Physiology and Center for Neuroscience 304-293-7316

Functional Characterization of Oocyte-specific Maternal Effect Genes Early embryonic loss is a major contributing factor to infertility in livestock species. The oocyte plays an active role in regulation of key aspects of the reproductive process required for fertility. Maternal mRNAs that accumulate in the oocyte during oogenesis play important roles during initial stages of embryonic development, before activation of the embryonic genome. A major objective of my laboratory is to enhance understanding of the functional contribution of novel oocyte-specific genes to early embryonic development and the mechanisms involved. Jianbo Yao Animal and Nutritional Sciences 304-293-1948
















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For more information on LIINC, please visit the website at: or contact Lindsay Emery directly at 304-293-0391

Made possible from the support of the Claude Worthington Benedum Foundation


Second Annual Bioscience and Biomedical Research Showcase Booklet  

This booklet includes attending industries as well as faculty abstracts.