whatâ&#x20AC;&#x2122;s new Cell lysis RT-qPCR kits Bio-Rad Laboratories has announced the launch of a rapid cell lysis kit that allows researchers to obtain reverse transcription quantitative PCR (RT-qPCR) data directly
Lysis and DNA purification system
from cultured cells without the need for
NucleoSpin isolation technology from
a separate RNA purification step. The
Macherey Nagel provides a lysis and DNA
SingleShot family of cell lysis RT-qPCR
purification system for nearly all types
kits provide high-quality gene expression
of food samples. Even low amounts of
results in less than 2 h.
partially degraded DNA can be purified
Available column isolation methods for purifying RNA are said to be time consuming and
from complex matrices including honey,
laborious, while other methods that enable RT-qPCR directly from cell lysates can damage
pollen, bread, flour, spices, cereals, meat,
the RNA and result in poor genomic DNA clearance. The kits eliminate such challenges
pharmaceutical tablets, cosmetic creams
and offer good reproducibility and accuracy of gene expression results. Minimal set-up and
and powders, and starter cultures.
pipetting steps create an automation-friendly workflow and, unlike other similar methods, the kits do not require an additional pipetting step to stop the cell lysis reaction.
Resulting eluates from the fast and easy protocol are ready to use for all
The kits are suited for high-throughput laboratories with large-volume workloads and for
types of subsequent detection methods,
researchers who are faced with a limited number of cells and require high accuracy in each
especially real-time and basic PCR
analysis. They include an RNA control template and qPCR assay to help researchers deter-
technology. Sensitivity is ensured as the
mine optimal cell number and lysate inputs for their RT-qPCR reactions.
system completely removes PCR inhibi-
The kits are validated for use with a wide variety of adherent and suspension cell lines
tors. The process can also be automated
and are offered in multiple formats, including one-step RT-qPCR and two-step RT-qPCR kits
on vacuum-based robotic workstations.
that are compatible with either SYBR Green or probe-based assays. They are also available as stand-alone cell lysis kits.
Scientifix Pty Ltd www.scientifix.com.au
Bio-Rad Laboratories Pty Ltd www.bio-rad.com
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