Kit for gene expression array target preparation
Affymetrix has introduced the GeneChip WT Pico Kit for gene
expression array target preparation from as little as 100 pg
spectrometer is an
of total RNA input. Working with as few as 10 cells, the kit
offers a high degree of flexibility and precision, enabling
analyses of samples too small for other methods as well
as the interrogation of small subpopulations of cells within
that combines JD-
larger samples. This is particularly important to the study
SU’s high-volume, high-precision optical coating technology with
of heterogeneous solid tumour samples, haematologic
innovation in optical system design and miniaturisation. The
malignancies and other precious samples.
spectrometer relies on the company’s linear variable filter (LVF)
For use with the Affymetrix GeneChip Whole-Transcriptome
technology as the dispersing element.
(WT) Arrays, the kit is compatible with small-sample isolation
The spectrometer contains a light source, collection optics,
techniques, including flow cytometry, laser capture microdis-
electronics and dispersing element in a housing that is <50 x 50
section and fine needle aspiration. The kit prepares targets
mm. The USB-powered device can be used in diffuse reflection,
from multiple sample types, including fresh and fresh frozen
transmission or transflection modes in a handful of configurations
tissues, cultured cells, FFPE specimens and whole blood
or as a process sensor.
samples without a globin mRNA reduction step. The use
The MicroNIR Pro features an intuitive software interface de-
of a single kit for multiple sample types is said to improve
signed for touch-screen devices. The software features method
the ability to better compare data from different samples.
development and real-time prediction capability for qualitative
The target preparation kit, combined with the GeneChip
or quantitative analysis. Also featured is a complete set of tools
Human Transcriptome Array 2.0 (HTA 2.0) and Transcriptome
enabling 21 CFR Part 11 compliance, USP 119 performance
Analysis Console Software, forms a solution for precise cell
verification, multilevel user management and access, customis-
subset analyses claimed to be impossible with traditional
able workflow, data storage and retrieval, and audit trail viewing.
whole-transcriptome analysis techniques, which require
Data collected with the system is easily exported to the em-
large numbers of cells and typically deliver measurement
bedded calibration development software powered by CAMO
averages. Measurement of all transcript isoforms, including
Software’s The Unscrambler X. Applications include pharmaceutical
long non-coding RNA transcripts, is made possible by the
(PAT), polymer and petrochemical analysis, food and agriculture.
high-resolution microarray-based gene expression profiling
Usage tests have been conducted in fresh seafood, chicken,
solution, which uses more than six million probes covering
raw materials, chemical analysis, narcotics and pharmaceuticals.
over 285,000 coding and non-coding transcripts and inter-
Raymax Applications Pty Ltd
rogates the entire length of each one.
Millennium Science Pty Ltd www.mscience.com.au
arium ultrapure ®
www.LabOnline.com.au | www.LifeScientist.com.au
-every lab deserves it
LAB+LIFE SCIENTIST - July 2015 | 37