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Next Generation Sequencing Libraries

Maike Paramor

This facility provides the preparation of DNA/RNA libraries for Next Generation Sequencing (NGS) projects to support SCI scientists. In recent years, the demand for high throughput sequencing methods has increased rapidly in the field of stem cell research. However, the technical challenge of library production is often daunting and time-consuming, and forms the major bottleneck for many projects. To accommodate this, the Institute has created a state-of-theart facility which produces NGS libraries on demand. This facility provides a fast turnaround for standard library preparation. Moreover, individual and non-standard methods are being developed as well. We strive to provide a local and flexible service to anybody within the Institute. Services  Help and advice with project planning  Transcriptome/RNA-seq libraries  Small RNA libraries  ChIP-seq libraries  DNA/amplicon libraries  individual projects and non-established methods will be considered Moreover, we offer the use of our Covaris for shearing of DNA/ RNA samples or chromatin samples. Training can be provided on request. Sequencing The sequencing itself is not done in-house. However, we are soon providing direct access to use the Illumina HiSeq sequencer in the CRI here in Cambridge. Access to a MiSeq and a Roche 454 is provided by the Biochemistry DNA sequencing facility. Currently available preparations/kits:  Illumina small RNA library kit  Nextflex directional RNA seq kit (Illumina)  Nextflex adapters for multiplexing up to 24 samples (Illumina)  NEBNext reagents for standard Illumina  DNA libraries and ChIP libraries  Covaris S2 instrument for DNA/RNA shearing and chromatin shearing Staff Maike Paramor 37

Cambridge Stem Cell Institute Brochure 2012  
Cambridge Stem Cell Institute Brochure 2012