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Flow Cytometry

Andy Riddell

Flow cytometry is a laser-based, biophysical technology employed in cell counting, cell sorting, biomarker detection and protein engineering, by suspending cells in a stream of fluid and passing them by an electronic detection apparatus. It allows simultaneous multiparametric analysis of the physical and chemical characteristics of thousands of particles every second. Flow cytometry is routinely used in the diagnosis of health disorders but has many other applications in basic research, clinical practice and clinical trials. A common variation is to physically sort particles based on their properties, so as to purify populations of interest. Services We have a multi-site facility comprising of expert-led highspeed cell sorters and self-use sorter/analyser equipment. Our services include sorting, assay design and training. Equipment Sorters: Beckman Coulter MoFlo 3 laser system with up to 12 parameters. BioRad S3 Bench to sorter with 2 lasers and 7 parameters. A BD Aria IIu sorter with 3 lasers and up to 12 parameters. Analysers: A BD Fortessa with 4 lasers and 20 parameters. A Beckman Coulter CyAn with 3 lasers and 10 parameters Training Training is given on the systems by request.

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Cambridge Stem Cell Institute Brochure 2012  
Cambridge Stem Cell Institute Brochure 2012