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Advanced Vector Design and Recombineering

Katharina Boroviak

Recombineering (recombination-mediated genetic engineering) enables fast and efficient construction of vectors for subsequent manipulation of mouse genomes. It is based on homologous recombination which is a type of genetic recombination in which nucleotide sequences are exchanged between two similar or identical molecules of DNA. It is used by cells to accurately repair harmful breaks that occur on both strands of DNA, known as double-strand breaks. As this technology is based on homologous recombination it is incredibly versatile and can be used to create vectors for protein tagging (N or C terminal), reporters, knock-in and (conditional) knockout. Our BAC recombineering system is based on Gateway (Invitrogen) and thus provides great flexibility due to its modularity. It also avoids the amplification of the reporter construct by PCR and is therefore less prone to the introduction of mutations. Services The Advanced Vector Design and Recombineering Facility provides Institute scientists with the following services:     

BAC recombineering TALENs CRISPRs Vector design for recombinant protein production Help and advice for PhD students and PostDocs when designing and cloning the vectors for their projects

The following services are currently being established:  Transposon mediated BAC-transgenesis

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Cambridge Stem Cell Institute Brochure 2012  
Cambridge Stem Cell Institute Brochure 2012