CURO Symposium 2017 Book of Abstracts

Page 95

Abstracts tests at different temperature and loading frequency conditions. Several State Highway Agencies (SHAs) have already created or are in the process of creating an |E*| database for the calibration and implementation of MEPDG. Georgia Department of Transportation (GDOT) has made a continued commitment to the performance enhancement of pavement and proactively calibrated and implemented the MEPDG methodology for the design of flexible pavement structures. However, the GDOT material input library includes |E*| based on only two sources of aggregate. Further, an |E*| library for Polymer Modified Asphalt (PMA) and Ground Tire Rubber (GTR) Asphalt mixtures has not been developed yet although the PMA mixtures are being used for high volume traffic roads in Georgia. The study will supplement the current |E*| database by extending the aggregate sources for different Superpave mixes and binder types. Secondly, three different laboratory fatigue tests will be performed on selected mixtures. Based on different fatigue test approaches, a local fatigue prediction model will be recommended for Georgia pavement. Finally, the effects on dynamic modulus and fatigue due to different asphalt mixtures will be determined.

Genetic Mapping of the Chromosome Variant K10-L2 in Maize Brianna Renee English, Foundation Fellow, CURO Research Assistant Dr. Kelly Dawe, Genetics, Franklin College of Arts and Sciences Meiosis is a process in which a diploid cell divides into four haploid daughter cells, capable of becoming the organism’s next generation. During the stage of anaphase, sister chromatids are pulled apart towards opposite spindle poles through microtubule attachment to the centromeres of the chromosomes. In maize, there are two forms of chromosome ten: a common normal version and a rare abnormal version which contains repetitive sequences called knobs. Knobs are normally inactive regions of the genome, but in the presence of the abnormal chromosome K10L2 they can act as neocentromeres. When active, these neocentromeres move towards the spindle poles ahead of the centromere during anaphase. This study is focused on constructing a genetic map of K10L2 and developing a better understanding of how neocentromeres function. Using fluorescent in situ hybridization to observe knobs, PCR to amplify candidate genes, and the observation of phenotypes unique to chromosome ten, we analyzed the combinations of markers from each test to generate a genetic map. This study will ultimately provide more information about the origins of K10L2 and will help in finding the location of the candidate gene.

In Vivo and In Vitro Activity Analysis of Acetylation on Fructose-1, 6Bisphosphatase in Salmonella enterica Akiel Gabriel Etienne, CURO Research Assistant Dr. Jorge Escalante-Semerena, Microbiology, Franklin College of Arts and Sciences

Effects of Asphalt Mix Characteristics on Dynamic Modulus and Fatigue Performance Austin Etheridge, CURO Research Assistant Dr. S. Sonny Kim, Environmental, Civil, Agricultural, and Mechanical Engineering, College of Engineering

Glucose is an important building block for cells of all domains of life. Not surprisingly, cells have evolved elaborate systems to acquire glucose from their environments. Because of the relevance of glucose to cell function, cells can also make glucose endogenously. The pathway dedicated to this purpose is known as gluconeogenesis (GNG). The enzyme fructose-1, 6-bisphosphatase (Fbp) plays a key role in GNG because Fbp generates glucose 1,6-biphosphate. Fbp function is regulated at many levels. Recently, we discovered that Fbp function may also be regulated by posttranslational modifications. Reverse Lysine Acetylation (RLA) is a post-translational modification that is conserved across all domains of life, and is used to modulate protein function. Recently, we showed that STM1857 acetylates

Developed under NCHRP 1-37A, the Mechanistic-Empirical Pavement Design Guide (MEPDG) provides three hierarchical levels of design inputs (i.e., levels 1, 2, and 3). This allows the designer to select the level of details of design inputs according to the level of importance of the project. The dynamic modulus |E*| is considered one of the fundamental asphalt mix properties and is obtained from a series of complex modulus 90