Abstracts Dr. Mandi M Murph, Pharmaceutical and Biomedical Sciences, College of Pharmacy
and H2 or formate instead of sugars, for nextgeneration biochemical productions. Our team is developing tools and methods for modulating protein expression in M. maripaludis, an archaeal model. Our focus is engineering the Ribosomal Binding Site (RBS). We use a mCherry reporter developed by our team previously to measure protein expression levels in a library of RBS mutants. We are now working to (1) determine the effects of mutations in the spacer region and the role of mRNA secondary structure, (2) expand our Archaeal Interlab study to encourage more iGEM teams to collaborate with us and standardize our fluorescence measurement protocol, and (3) continue metabolic modeling to evaluate the effect of alternate carbon sources on cell growth and geraniol production in M. maripaludis.
Current therapeutic options aimed at cancer diagnoses include targeted therapy, immunotherapy, surgery, chemotherapy, and radiation to eliminate the present tumors. Each class of drugs strives to combat the cancer cellâ&#x20AC;&#x2122;s ability to hijack their surrounding healthy systems, and therefore, a multifactorial approach to patient therapy is often ideal. However, 70% of patients do not respond to initial chemotherapy and the five-year survival rate for patients who display resistant behavior ranges from 10 to 30%. Cancer cells are able to prevent drug influx and therefore, buildup of chemoresistance often results in therapeutic failure. In this study, the cytotoxic effects of the provided compounds on are observed. We hypothesize that exposure of various melanoma and ovarian cancer cell lines to these potential cytotoxic compounds will greatly reduce cell viability, and possibly yield a novel therapeutic agent to combat malignant tumor growth. Alterations of functional groups on the original set of compounds may also enhance or decrease cytotoxic effects and is currently being explored through creating variations of successfully tested candidates. We utilized cytotoxicity assays depicted as dose response curves to represent our results and determine IC50 and statistical values. The three most effective compounds were D5, D9 and C2 as a majority of their IC50 values fell under the ideal 10 ÂľM across the different cell lines. For future work, we plan to continue testing variations of the three successful compounds to yield the most potent candidate for in vivo testing.
A Method for Enrichment of Maize Stem Cells and Leaf Primordia Krishna Patel Eva Lauren Rodriguez Dr. Xiaoyu Zhang, Plant Biology, Franklin College of Arts and Sciences Plant development is characterized by continuous organ formation and growth throughout the life cycle. This is primarily achieved via two small groups of self-renewing stem cells at the shoot and root apical meristems (SAM and RAM, respectively). In the SAM, stem cells are maintained in a central zone and daughter cells move laterally to peripheral zones where they differentiate into lateral organs, such as leaves. Despite the biological importance of plant stem cell maintenance and differentiation, the underlying mechanisms of stem cell selfmaintenance and differentiation remain outstanding questions in plant biology. This gap in our understanding derives largely from the technical difficulty of performing chromatin analyses on the small number of cells in the SAM. To resolve this gap, we will use cell-type specific fluorescent lines, coupled with fluorescence-activated cell sorting, to acquire homogenous samples of stem cells and cells of young leaf primordia. We plan to use the Assay for Transposase-Accessible Chromatin (ATAC) to map accessible chromatin and transcription factor binding sites genome-wide. The ATAC-seq uses a hyperactive Tn5 transposase to insert Illumina sequencing adapters into sterically accessible
Building the Genetic Tools to Make Methanococcus Maripaludis the NextGeneration Model Chassis for Biochemical Production Hirel B Patel, CURO Research Assistant Ben Park, Kyler Herrington, Ghazal Motakef Dr. William Whitman, Microbiology, Franklin College of Arts and Sciences Current bacterial chassis use expensive sugars as feedstocks, which limits profitability. Using the archaeal model Methanococcus, we are developing an archaeal chassis that feeds on inexpensive CO2 184