Page 1

LC Columns USLC® Columns .......................................................137–139 U.S. Pharmacopeia Cross-Reference .........................140 Tables and Reference......................................................141 Columns for UHPLC ...............................................142–148 Columns for HPLC ..................................................149–154 Columns for Bioseparations ...............................155–157 Application-Specific Columns ...........................158–163 Prep Columns...........................................................164–167 Bulk Packing Materials....................................................167 UHPLC Filters ......................................................................168 HPLC Guard Cartridge Systems ........................169–171 LC Column & Detector Test Mixes .............................449 LC Chromatograms................................................690–721

LC Columns Teknolab AB, Energigatan 5A, 434 37 Kungsbacka Tel: +46 (0)300-568 660 • Fax: +46 (0)300-568 669 • info@teknolab.se


LC COLUMNS

USLC® Columns

RESTEK

USLC®

Ultra Selective Liquid Chromatography www.restek.com/uslc

Ultra Selective Liquid Chromatography Technology Choose Columns Fast. Develop Methods Faster. What is Ultra Selective Liquid Chromatography (USLC®) technology? This technique is the directed application of orthogonal selectivity—the most influential factor affecting peak separation, or resolution—to provide the practicing chromatographer with the best tools for choosing columns fast and developing methods faster. Through our extensive study of reversed-phase chromatography Restek created the widest range of selectivity in the industry using just four unique stationary phases: the USLC® column set. We also defined a simple approach to choosing a column with the appropriate selectivity for any application.

Selectivity Drives Separations Quickly and effectively resolve analytes by understanding and controlling selectivity through USLC® technology. One of the most significant, yet least understood, steps of method development is finding the proper stationary phase for a particular separation. As sample complexity increases, achieving adequate resolution between matrix components and target analytes becomes more difficult. Despite Equation 1: Selectivity has the greatest recent advancements in column format, such as sub-2-micron packings and mathematical effect on resolution. pellicular particles, resolution can still be difficult to obtain because, while these formats can increase chromatographic efficiency and analysis speed, they do not R= N x (k/[k+1]) x (α-1) significantly influence resolution. Selectivity, as shown in Equation 1, is the single Efficiency Retention Factor Selectivity most powerful factor affecting resolution, and it is largely dependent upon stationary phase composition.

¼

Real Diversity in Phase Chemistry A small set of defined orthogonal columns means faster separations and more robust methods. While numerous bonded phases are available for reversed-phase chromatography, many (e.g., C8 and C18) are similar and offer only moderate changes in retention, rather than significant differences in selectivity. Method development is less laborious and timeconsuming when you use a full range of column selectivities, including orthogonal phase chemistries like polar-embedded, phenyl, and fluorophenyl columns. Restek has led the development of the unique USLC® column set across these phase classes to provide analysts with a more effective range of column selectivities and innovative column chemistries for method development. The USLC® column set (Figure 1) provides the widest range of reversed-phase selectivity available with just four columns and can be used to guide proper stationary phase selection—the least understood yet most significant part of method development.

Figure 1: Restek columns offer the widest range of unique and effective phase chemistries to aid the chromatographer in choosing columns fast and developing methods faster. Restek USLC® Phase (column class)

Aqueous C18 (alkyl)

Biphenyl (phenyl)

IBD (polar embedded)

F

P Si

P

CH3

Si

CH3

CH3

Si

PFP Propyl (fluorophenyl) F

F

CH3

CH3

F F

Si

CH3

O

O

O

Aqueous C18

Biphenyl

IBD

PFP Propyl

Ligand Type

Proprietary polar modified and functionally bonded C18

Unique Biphenyl

Proprietary polar functional embedded alkyl

Fluorophenyl

Properties

• General-purpose with a wellbalanced retention profile.

O O

• Compatible with 100% aqueous mobile phases. • Ideal for multi-component LC-MS analyses.

• Increased retention for dipolar, unsaturated, or conjugated solutes.

• Increased retention for acids and water-soluble compounds.

• Increased retention for both charged bases and electronegative compounds.

• Enhanced selectivity when used with protic (methanol) mobile phase.

• Compatible with 100% aqueous mobile phases.

• Capable of both reversedphase and HILIC separations.

• Capable of both reversed-phase and HILIC separations.

• Ideal for increasing sensitivity and selectivity in LC-MS analyses.

• Ideal for increasing sensitivity and selectivity in LC-MS analyses.

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137


LC COLUMNS

USLC® Columns Evaluating and Extending Selectivity The Restek USLC® column set offers the highest range of alternate selectivity available. The diverse selectivity provided by USLC® columns can be demonstrated empirically using the hydrophobic-subtraction model [1]. This model is a novel procedure for characterizing selectivity that uses test probes to define the solute and stationary phase interactions in reversed-phase separations. Restek is leading the commercial application of this model by implementing it in the development of USLC® bonded phases. To evaluate phase selectivity using the hydrophobic-subtraction model, the retention characteristics of the solute probes are compared across different phases relative to a C18 benchmark with all columns using the same silica base. The resulting scatter plot is an excellent way to visualize selectivity. Stationary phases with similar selectivity show high linearity when graphed. However, stationary phases with alternate selectivity—even orthogonality—produce significant scatter around the regression line. The high degree of scatter shown in Figure 2 shows just how diverse the phases in the USLC® column set are. When we quantify column selectivity based on this correlation by calculating the selectivity (S) statistic [2], the resulting value of 46.7 shows that the USLC® column set truly has the highest range of selectivity available.

Figure 2: Restek has extended the selectivity range for commercially available columns and defined a column set—the four USLC® phases—that is ideal for fast column choice and faster method development. 14.00

y = 0.4755x + 0.7619 R2 = 0.7819

12.00

S = 46.7

Orthogonal phases

k Orthogonal phase

10.00 Aqueous C18

8.00

IBD

6.00

Biphenyl PFPP

4.00 2.00 0.00 0.00

All columns were tested using the same silica support. 2.00

4.00

6.00

8.00

10.00

12.00

14.00

16.00

k C18 phase

References [1] L.R. Snyder, J.W. Dolan, P.W. Carr, The Hydrophobic-Subtraction Model of Reversed-Phase Column Selectivity, J. Chromatogr. A 1060 (2004) 77. [2] U.D. Neue, J.E. O’Gara, A. Mendez, Selectivity in Reversed-Phase Separations influence of the Stationary Phase, J. Chromatogr. A 1127 (2006) 161.

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ar

If you know what type of column interaction you need for your analysis, use these charts to select your USLC® column.

H-B o

Po l

Each USLC® column is optimized for a different chemical interaction. The pie chart provided for each USLC® stationary phase in this catalog (Figure 3) identifies the same four molecular interactions (color coded to correspond to the retention of a different solute type). The more rings shown for a given interaction, the more significant a role it plays in defining solute retention.

on rsi

ing nd

Put simply, selectivity is the retention of one compound relative to another. Therefore, because solutes will be retained to different degrees by different molecular interactions, we can fundamentally define a column’s selectivity based on the molecular interactions it delivers.

Figure 3: Sample USLC® Column Interaction Profile

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USLC® Column Interaction Profile

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LC COLUMNS

USLC® Columns USLC® Method Development Toolbox • Ultra Selective Liquid Chromatography (USLC®) method development toolbox contains all four USLC® stationary phases in one convenient package. • Available for UHPLC (1.9 μm) and HPLC (3 or 5 μm) in 50, 100, or 150 mm lengths. • Included selection guide makes it even easier to pick the right column the first time. Description Pinnacle DB USLC Method Development Toolbox Pinnacle DB USLC Method Development Toolbox Ultra USLC Method Development Toolbox Ultra USLC Method Development Toolbox Ultra USLC Method Development Toolbox Ultra USLC Method Development Toolbox Ultra USLC Method Development Toolbox Ultra USLC Method Development Toolbox

Size 1.9 μm, 2.1 mm x 50 mm 1.9 μm, 2.1 mm x 100 mm 3 μm, 2.1 mm x 50 mm 3 μm, 2.1 mm x 100 mm 3 μm, 3.0 mm x 100 mm 5 μm, 2.1 mm x 50 mm 5 μm, 2.1 mm x 100 mm 5 μm, 4.6 mm x 150 mm

Includes (1) each: Biphenyl (9409252), Aqueous C18 (9418252), IBD (9425252), PFP Propyl (9419252) (1) each: Biphenyl (9409212), Aqueous C18 (9418212), IBD (9425212), PFP Propyl (9419212) (1) each: Biphenyl (9109352), Aqueous C18 (9178352), IBD (9175352), PFP Propyl (9179352) (1) each: Biphenyl (9109312), Aqueous C18 (9178312), IBD (9175312), PFP Propyl (9179312) (1) each: Biphenyl (910931E), Aqueous C18 (917831E), IBD (917531E), PFP Propyl (917931E) (1) each: Biphenyl (9109552), Aqueous C18 (9178552), IBD (9175552), PFP Propyl (9179552) (1) each: Biphenyl (9109512), Aqueous C18 (9178512), IBD (9175512), PFP Propyl (9179512) (1) each: Biphenyl (9109565), Aqueous C18 (9178565), IBD (9175565), PFP Propyl (9179565)

qty.

cat.#

kit

25800

kit

25807

kit

25801

kit

25802

kit

25803

kit

25804

kit

25805

kit

25806

USLC® Columns Choose Columns Fast. Develop Methods Faster. www.restek.com/uslc

Mobile Phase Management 101 Neatly Keep Mobile Phase Lines Where They Belong

Transfer and Filter Mobile Phase in a Single Step

Hub-Cap

Hub-Cap Filters

See page 318.

See page 319.

Hub-Cap (assembly of the bottle cap and plug)

Reduce Solvent Waste Last Drop Filter

Avoid Messy Spills Around Mobile Phase Waste Containers

See page 320.

Waste Overflow Indicator

Prepare and Maintain Mobile Phases Without Dissolved Gas or Unnecessary Costs

See page 319.

Mobile Phase Sparge Filter See page 320.

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LC COLUMNS

U.S. Pharmacopeia Cross Reference U.S. Pharmacopeia Cross Reference L1

Octadecyl silane chemically bonded to porous silica or ceramic microparticles; 1.7 to 10 μm in diameter, or a monolithic rod. Pinnacle® DB C18 (p. 143), Pinnacle® DB Aqueous C18 (p. 144), Ultra C18 (p. 149), Ultra Aqueous C18 (p. 151), Viva C18 (p. 155)

L3

Porous silica particles; 5 to 10 μm in diameter. Pinnacle® DB Silica (p. 147 ), Ultra Silica (p. 154), Viva Silica (p. 157 )

L7

Octylsilane chemically bonded to totally porous silica particles; 1.7 to 10 μm in diameter. Pinnacle® DB C8 (p. 143), Ultra C8 (p. 149), Viva C8 (p. 155)

L8

An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support; 3 to 10 μm in diameter. Ultra Amino (p. 154)

L10

Nitrile groups chemically bonded to porous silica particles; 3 to 10 μm in diameter. Pinnacle® DB Cyano (p. 147 ), Ultra Cyano (p. 154)

L11

Phenyl groups chemically bonded to porous silica particles; 1.7 to 10 μm in diameter. Pinnacle® DB Biphenyl (p. 145), Ultra Aromax (p. 151), Ultra Biphenyl (p. 152), Viva Biphenyl (p. 156)

L13

Trimethylsilane chemically bonded to porous silica particles; 3 to 10 μm in diameter. Ultra C1 (p. 150)

L26

Butyl silane chemically bonded to totally porous silica particles; 3 to 10 μm in diameter. Ultra C4 (p.150), Viva C4 (p.156)

L43

Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer; 5 to 10 μm in diameter. Pinnacle® DB PFP Propyl (p. 146), Ultra PFP Propyl (p. 153), Viva PFP Propyl (p. 156)

L68

Spherical, porous silica; 100 μm or less in diameter, the surface of which has been covalently modified with alkyl amide groups and not end-capped. Pinnacle® DB IBD (p. 146), Ultra IBD (p. 153)

®

EXP fittings

hand-tight

Reusable fittings for easy, yet reliable HPLC & UHPLC connections • Hand-tight fitting style achieves effortless HPLC seals—no tools needed! • Both hand-tight and hex-head styles wrench-tighten for reliable UHPLC use. • Patented ferrule can be installed repeatedly without compromising high-pressure seal. • Hybrid design combines the durability of titanium with the sealing ability of PEEK. • Cutting-edge system provides ZDV (zero dead volume) connection to any 10-32 female port. • Compatible with 1/16" PEEK and stainless steel tubing.

See page 313. www.restek.com/exp hex-head

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LC COLUMNS

Tables and Reference Optimal Flow Rates for Various Particle Diameters and Column Internal Diameters Column ID (mm)

Common Classifications for LC Columns by Internal Diameter

Optimal flow rate (mL/min) 1.9 μm dp — — 1.12 0.55 —

4.6 3.2 3.0 2.1 1.0

3 μm dp 1.50 0.73 0.65 0.31 0.07

Classification Capillary Micro bore Narrow bore Standard bore Semi-prep Prep

5 μm dp 1.00 0.50 0.40 0.20 0.05

Internal Diameter <1.0 mm ID 1.0 mm ID 2.1–3.0 mm ID 3.2–4.6 mm ID 10–21.2 mm ID 30–50 mm ID

HPLC Pump Pressure Conversion Table Pressure 1 psi = 1 atm = 1 kg/cm2 = 1 torr = 1 kPa = 1 bar = 1 in Hg =

psi 1 14.696 14.223 0.0193 0.1450 14.5038 0.49612

kg/cm2 0.0703 1.0332 1 0.00136 0.0102 1.0197 0.0345

atm 0.068 1 0.967 0.00132 0.00987 0.9869 0.0334

torr 51.713 760 735.5 1 7.52 751.88 25.400

kPa 6.8948 101.32 98.06 0.1330 1 100 3.376

bar 0.06895 1.0133 0.9806 0.00133 0.0100 1 0.03376

inches Hg 2.0359 29.921 28.958 0.0394 0.2962 29.5300 1

To convert a pressure, multiply the units in the left-most column by the conversion factors listed in the columns to the right. For example: 10 psi x 0.068 = 0.68 atm 10 bar x 29.5300 = 295.300 inches Hg

e

am

xid

yl s

Miscible/Water-Miscible Solvents ofu

nol

Immiscible Solvents

ydr

tha

Miscible Solvents

ran

pyl pro

Iso

Eth

ano

l

alc

xan

oho

e

l

eth Dio

ride hlo

e l

rac

zen

ne chl oro e hlo tha rom ne Eth eth yl a ane cet Eth ate yl e the He r pta ne He xan e

one ket

rt-b

hyl

l te

Xyl ene

Tol uen e

Pen

tan

e

l et

Me

thy

thy

Me

Iso oct

ane

uty l

eth

er

Dic

1,2

-Di

loh exa

Cyc

oro f Chl

Car

bon

orm

tet

ano But

Ben

Wa

ter

rah

Me

Do we have the reference info for this???? Tet

Dim

eth

ulfo

yl f

orm

le itri

ton Ace

ton Ace

Acetone Acetonitrile Dimethyl formamide Dimethyl sulfoxide Dioxane Ethanol Isopropyl alcohol Methanol Tetrahydrofuran Water Benzene Butanol Carbon tetrachloride Chloroform Cyclohexane 1,2-Dichloroethane Dichloromethane Ethyl acetate Ethyl ether Heptane Hexane Isooctane Methyl tert-butyl ether Methyl ethyl ketone Pentane Toluene Xylene

Dim

e

ide

Solvent Miscibility and Solubility

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141


LC COLUMNS

Columns for UHPLC Pinnacle® DB Columns: 1.9, 3, or 5 μm particles; 140 Å pore size Restek Pinnacle® DB columns are built for optimal UHPLC performance. Pinnacle® DB columns are 100% manufactured by Restek in our Bellefonte, Pennsylvania, facility. Because performance begins with the support, our Pinnacle® DB UHPLC columns start with base-deactivated spherical silica that is optimized for UHPLC stability. From there, we bond them with a wide variety of phases to give chromatographers a stable and selective column. Get the most out of your UHPLC system. Combine selectivity and efficiency by using Restek Pinnacle® DB UHPLC columns.

Get Even More Out of Your UHPLC System Combine UHPLC Efficiency With Restek Selectivity

Restekmanufactured silica

The following stationary phases are available for UHPLC:

USLC® Columns:

Conventional Columns:

Phase Page Aqueous C18 (USLC phase) ............144 Biphenyl (USLC Phase) ......................145 IBD (USLC Phase) ................................146 PFP Propyl (USLC Phase) ..................146

Phase Page C18 ...........................................................143 C8 .............................................................143 Cyano ......................................................147 PAH ..........................................................148 Silica ........................................................147

www.restek.com/LC-Columns

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LC COLUMNS

Columns for UHPLC Restekmanufactured silica

Pinnacle® DB C18 Columns (USP L1) ChromatographicP roperties

The general-purpose Restek C18 is a conventional monomeric octadecylsilane column suitable for analyses of a wide range of compounds from acidic through slightly basic. Length 1.9 μm Columns 30 mm 50 mm 100 mm 3 μm Columns 30 mm 50 mm 100 mm 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

1.0 mm ID cat.#

2.1 mm ID cat.#

3.0 mm ID cat.#

Column Characteristics: particle size:

4.6 mm ID cat.#

9414232 9414252 9414212

CH3 9414331 9414351 9414311

9414332 9414352 9414312

941433E 941435E 941431E

9414335 9414355 9414315

9414531 9414551 9414511 9414561 9414521 9414571

9414532 9414552 9414512 9414562 9414522 9414572

941453E 941455E 941451E 941456E 941452E 941457E

9414535 9414555 9414515 9414565 9414525 9414575

Si

CH3

O

1.9 μm, 3 μm, or 5 μm, spherical pore size: 140 Å carbon load: 11% end-cap: yes pH range: 2.5 to 8 temperature limit: 80 °C USP phase code: L1 phase category: C18, octadecylsilane ligand type: monomeric C18

C18

Restekmanufactured silica

Pinnacle® DB C8 Columns (USP L7) ChromatographicP roperties

Our C8 is a conventional monomeric octylsilane column offering a shorter alkyl chain to provide less hydrophobic retention and improved basic peak shape over a traditional C18 phase. Like our C18, this general-purpose Restek C8 is suitable for a wide range of compounds from acidic through slightly basic. Length 1.9 μm Columns 30 mm 50 mm 100 mm 3 μm Columns 30 mm 50 mm 100 mm 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

1.0 mm ID cat.#

2.1 mm ID cat.#

3.0 mm ID cat.#

4.6 mm ID cat.#

Column Characteristics: particle size:

CH3

Si O

9413232 9413252 9413212 9413331 9413351 9413311

9413332 9413352 9413312

941333E 941335E 941331E

9413335 9413355 9413315

9413531 9413551 9413511 9413561 9413521 9413571

9413532 9413552 9413512 9413562 9413522 9413572

941353E 941355E 941351E 941356E 941352E 941357E

9413535 9413555 9413515 9413565 9413525 9413575

CH3

1.9 μm, 3 μm, or 5 μm, spherical pore size: 140 Å carbon load: 6% end-cap: yes pH range: 2.5 to 8 temperature limit: 80 °C USP phase code: L7 phase category: C8, octylsilane ligand type: monomeric C8

C8

also available Trident Direct Guard Column System See page 169.

Looking for an equivalent column? Restek has extensively studied column selectivity and can provide you with an accurate recommendation. Please contact Restek Technical Support or your local Restek representative.

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LC COLUMNS

Columns for UHPLC Restekmanufactured silica

Pinnacle® DB Aqueous C18 Columns (USP L1) ColumnC haracteristics:

Chromatographic Properties

particle size:

1.9 μm, 3 μm, or 5 μm, spherical pore size: 140 Å carbon load: 6% pH range: 2.5 to 8 temperature limit: 80 °C USP phase code: L1 phase category: modified C18 ligand type: proprietary polar modified and functionally bonded C18

The Restek Aqueous C18 is a rugged, reversed-phase column with a well-balanced retention profile. It can effectively retain more types of solutes than a conventional C18 and is ideal for multi-component LC-MS analyses. The general-purpose Aqueous C18 boasts high reproducibility and compatibility with many mobile phase conditions—even 100% aqueous and acidic. And when used with a gradient, it eliminates the all-too-common issue of multiple compounds eluting near the column void time. Si O O

Aqueous C18 USLC® Column Interaction Profile H-B o

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Dis pe

(See page 138 for more information.)

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P Length 1.9 μm Columns 30 mm 50 mm 100 mm 3 μm Columns 30 mm 50 mm 100 mm 150 mm 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

1.0 mm ID cat.#

2.1 mm ID cat.#

3.0 mm ID cat.#

4.6 mm ID cat.#

9418232 9418252 9418212 9418331 9418351 9418311 9418361

9418332 9418352 9418312 9418362

941833E 941835E 941831E 941836E

9418335 9418355 9418315 9418365

9418531 9418551 9418511 9418561 9418521 9418571

9418532 9418552 9418512 9418562 9418522 9418572

941853E 941855E 941851E 941856E 941852E 941857E

9418535 9418555 9418515 9418565 9418525 9418575

Pesticides on Pinnacle® DB Aqueous C18 (LC-MS/MS, ESI+) Column

➲ See page 717 for compound listing.

Dimensions: Particle Size: Pore Size: Temp.: Sample Diluent: Conc.: Inj. Vol.: Mobile Phase A: B:

Pinnacle® DB Aqueous C18 (cat.# 9418252) 50 mm x 2.1 mm ID 1.9 μm 140 Å 35 °C multicomponent pesticide standard water 33.3 ppb each pesticide 5 μL 10 mM NH4OAc in water 10 mM NH4OAc in methanol

Time (min) %B 0 10 1 10 8 90 10 90 11 10 Flow: 0.60 mL/min Max Pressure: ~517 bar Detector Applied Biosystems/MDS Sciex LC-MS/MS Model #: 4000 QTRAP® LC-MS/MS system Ion Source: TurboIonSpray® Ion Spray Voltage: 5 kV Gas 1: 40 psi (275.8 kPa) Gas 2: 60 psi (413.7 kPa) Source Temp.: 500 °C Instrument Shimadzu UFLCXR

LC_FF0479A

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LC COLUMNS

Columns for UHPLC Restekmanufactured silica

Pinnacle® DB Biphenyl Columns (USP L11)

Column Characteristics:

ChromatographicP roperties The Restek Biphenyl offers a greater degree of dispersion than conventional phenyls and a greater degree of polarizability than phenyl hexyls, creating higher selectivity and a greater range of usability. Because of these heightened interactions, this column shows substantial increases in retention—especially for dipolar, unsaturated, or conjugated solutes—and enhanced orthogonal selectivity when using methanol mobile phases. It is ideal for increasing sensitivity and selectivity in LC-MS analyses. 2.1 mm ID cat.#

3.0 mm ID cat.#

4.6 mm ID cat.#

CH3

Si

1.9 μm, 3 μm, or 5 μm, spherical pore size: 140 Å carbon load: 8% end-cap: yes pH range: 2.5 to 8 temperature limit: 80 °C USP phase: L11 phase category: phenyl ligand type: unique Biphenyl

CH3

O

Biphenyl

9409232 9409252 9409212

USLC® Column Interaction Profile

9409535 9409555 9409515 9409565 9409525 9409575

nge

940953E 940955E 940951E 940956E 940952E 940957E

H-B o

ab

ha

9409532 9409552 9409512 9409562 9409522 9409572

on rsi

xc

9409531 9409551 9409511 9409561 9409521 9409571

(See page 138 for more information.)

Dis pe

9409335 9409355 9409315 9409365

ing nd

940933E 940935E 940931E 940936E

iz

9409332 9409352 9409312 9409362

ar

9409331 9409351 9409311 9409361

Po l

Length 1.9 μm Columns 30 mm 50 mm 100 mm 3 μm Columns 30 mm 50 mm 100 mm 150 mm 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

1.0 mm ID cat.#

particle size:

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Sulfur Antibiotics by UHPLC on Pinnacle® DB Biphenyl

LC_GN0532 Column Dimensions: Particle Size: Pore Size: Temp.: Sample Diluent: Conc.: Inj. Vol.: Mobile Phase A: B:

Flow: Detector Instrument

Pinnacle® DB Biphenyl (cat.# 9409212) 100 mm x 2.1 mm ID 1.9 μm 140 Å 25 °C 0.1% Formic acid in water 50 μg/mL 2 μL 0.1% Formic acid in water 0.1% Formic acid in acetonitrile Time (min) %A 0 95 8 60 0.4 mL/min UV/Vis @ 265 nm Shimadzu UFLCXR

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11.

Peaks Sulfanilamide Sulfadiazine Sulfapyridine Sulfathiazole Sulfamerazine Sulfamethazine Sulfachlorpyridazine Sulfadoxine Sulfisoxazole Sulfadimethoxine Sulfaquinoxaline

RT (min) 1.55 3.74 4.09 4.24 4.35 4.91 5.87 5.99 6.37 7.14 7.40

%B 5 40

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LC COLUMNS

Columns for UHPLC Restekmanufactured silica

Pinnacle® DB IBD UHPLC Columns (USP L68) ColumnC haracteristics: particle size: pore size: end-cap: pH range: temperature limit: USP phase code: phase category:

1.9 μm, spherical 140 Å no 2.5 to 8 80 °C L68 polar-embedded alkyl proprietary CH3 polar functional embedded alkyl

ligand type:

ChromatographicP roperties The Restek IBD is a polar-embedded column that acts as a strong hydrogen bonder and may be the most versatile column available today. With a unique polar group, this column is very retentive and selective for acids. It also provides symmetrical peak shape for strong bases. Restek’s IBD is compatible with 100% aqueous mobile phases and can be used under reversedphase or HILIC conditions to retain very polar, ionic compounds in highly organic mobile phases.

P Si

CH3

O

2.1 mm ID cat.#

Length 1.9 μm Columns 30 mm 50 mm 100 mm

IBD

9425232 9425252 9425212

USLC® Column Interaction Profile H-B o

on rsi

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xc

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Po l

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ing nd

Dis pe

(See page 138 for more information.)

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Restekmanufactured silica

Pinnacle® DB PFP Propyl Columns (USP L43) ColumnC haracteristics: 1.9 μm, 3 μm, or 5 μm, spherical pore size: 140 Å carbon load: 6% F end-cap: yes pH range: 2.5 to 8 F temperature limit: 80 °C USP phase code: L43 phase category: fluorophenyl CH3 propyl ligand type: pentafluorophenyl propyl

ChromatographicP roperties

particle size:

F F F Si O

PFP Propyl USLC® Column Interaction Profile H-B o

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Dis pe

(See page 138 for more information.)

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CH3

The Restek PFP Propyl is a great choice for the retention and selectivity of charged bases, electronegative compounds, and amine-containing compounds. Unlike a conventional cyano column, however, the Restek PFP Propyl is much more amenable to LC-MS because it is more reliable and efficient with acidic mobile phases. This versatile column is also compatible with highly aqueous mobile phases and HILIC separations.

Length 1.9 μm Columns 30 mm 50 mm 100 mm 3 μm Columns 30 mm 50 mm 100 mm 150 mm 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

1.0 mm ID cat.#

2.1 mm ID cat.#

3.0 mm ID cat.#

4.6 mm ID cat.#

9419232 9419252 9419212 9419331 9419351 9419311 9419361

9419332 9419352 9419312 9419362

941933E 941935E 941931E 941936E

9419335 9419355 9419315 9419365

9419531 9419551 9419511 9419561 9419521 9419571

9419532 9419552 9419512 9419562 9419522 9419572

941953E 941955E 941951E 941956E 941952E 941957E

9419535 9419555 9419515 9419565 9419525 9419575


LC COLUMNS

Columns for UHPLC Restekmanufactured silica

Pinnacle® DB Cyano Columns (USP L10) ChromatographicP roperties

The Restek Cyano is a traditional monomeric cyanopropylsilane that is recommended for assays where alternate selectivity, or confirmation, to a C18 or C8 column is desired. It can be used in normal-phase, reversed-phase (best with mobile phase pH between 5 and 7), and HILIC modes. It is an excellent choice for the analysis of protonated bases. Length 1.9 μm Columns 30 mm 50 mm 100 mm 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

1.0 mm ID cat.#

2.1 mm ID cat.#

3.0 mm ID cat.#

4.6 mm ID cat.#

9416232 9416252 9416212 9416531 9416551 9416511 9416561 9416521 9416571

9416532 9416552 9416512 9416562 9416522 9416572

N

C CH3

Si

particle size:

CH3

O

Cyano 941653E 941655E 941651E 941656E 941652E 941657E

Column Characteristics: 1.9 μm or 5 μm, spherical pore size: 140 Å carbon load: 4% end-cap: yes pH range: 2.5 to 8 temperature limit: 80 °C USP phase code: L10 phase category: cyano ligand type: cyanopropylsilane

9416535 9416555 9416515 9416565 9416525 9416575

Restekmanufactured silica

Pinnacle® DB Silica Columns (USP L3) ChromatographicP roperties

Base-deactivated spherical silica is useful for normal-phase or HILIC separations. Length 1.9 μm Columns 30 mm 50 mm 100 mm 3 μm Columns 30 mm 50 mm 100 mm 150 mm 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

1.0 mm ID cat.#

2.1 mm ID cat.#

3.0 mm ID cat.#

4.6 mm ID cat.#

OH Si O O O

9410232 9410252 9410212 9410331 9410351 9410311 9410361

9410332 9410352 9410312 9410362

941033E 941035E 941031E 941036E

9410335 9410355 9410315 9410365

9410531 9410551 9410511 9410561 9410521 9410571

9410532 9410552 9410512 9410562 9410522 9410572

941053E 941055E 941051E 941056E 941052E 941057E

9410535 9410555 9410515 9410565 9410525 9410575

Silica

Column Characteristics: particle size:

1.9 μm, 3 μm, or 5 μm, spherical pore size: 140 Å end-cap: no pH range: 2.5 to 8 temperature limit: 80 °C USP phase code: L3 phase category: bare silica ligand type: none

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147


LC COLUMNS

Columns for UHPLC Restekmanufactured silica

Pinnacle® DB PAH UHPLC Columns ChromatographicP roperties

Specifically designed to resolve complex mixtures of polycyclic aromatic hydrocarbons (PAHs). Get complete resolution of all 16 EPA 610 PAHs, plus two other routinely analyzed PAH compounds, in less than five minutes to greatly reduce run times and increase sample throughput.

ColumnC haracteristics: particle size: pore size: end-cap: pH range: temperature limit:

1.9 μm, spherical 140 Å no 2.5 to 8 80 °C

2.1 mm ID cat.#

Length 1.9 μm Columns 30 mm 50 mm 100 mm

9470232 9470252 9470212

PAH Mix on Pinnacle® DB PAH 8

12

7

13

11 6 1

0.00

0.50

1.00

Column Dimensions: Particle Size: Pore Size: Temp.: Sample Diluent: Conc.: Inj. Vol.:

15

9 10 2

1.50

16

3 4 5

2.00

18

14

2.50 Time (min)

Pinnacle® DB PAH (cat.# 9470252) 50 mm x 2.1 mm ID 1.9 μm 140 Å 30 °C EPA Method 8310 PAH Mixture (cat.# 31841) acetonitrile 10 μg/mL 1 μL

3.00

Mobile Phase A: B:

Max Pressure: Detector Instrument

3.50

4.00

17

4.50

5.00 LC_EV0521

water acetonitrile Time (min) Flow (mL/min) %A 0 0.8 60 2 0.8 40 4 0.8 0 4.5 0.8 0 4.51 0.8 60 5 0.8 60 724 bar Photo diode array @ 254, 4.8 nm Waters

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18.

Peaks Naphthalene Acenaphthylene 1-Methylnaphthalene 2-Methylnaphthalene Acenaphthene Fluorene Phenanthrene Anthracene Fluoranthene Pyrene Benzo[a]anthracene Chrysene Benzo[b]fluoranthene Benzo[k]fluoranthene Benzo[a]pyrene Dibenzo[a,h]anthracene Benzo[ghi]perylene Indeno[1,2,3-cd]pyrene

%B 40 60 100 100 40 40

All the Right Tools— All in One Toolbox Get all four USLC® stationary phases in one convenient package. • Available for UHPLC (1.9 μm) and HPLC (3 or 5 μm) in 50, 100, or 150 mm lengths. • Included selection guide makes it even easier to pick the right column the first time.

See page 139. www.restek.com/uslc

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LC COLUMNS

Columns for HPLC Ultra HPLC Columns: 3 or 5 μm particles; 100 Å pore size The Ultra line represents Restek’s broadest selection of stationary phases on a single silica support. Made of high-purity, type-B silica that minimizes activity and creates high-density bonding, these columns are designed for selective and reliable HPLC applications.

Ultra C18 Columns (USP L1) ChromatographicP roperties The general-purpose Restek C18 is a conventional monomeric octadecylsilane column suitable for analyses of a wide range of compounds from acidic through slightly basic. Length 3 μm Columns 30 mm 50 mm 100 mm 150 mm 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

1.0 mm ID cat.# 9174331 9174351 9174311 9174361

2.1 mm ID cat.# 9174332 9174352 9174312 9174362

3.0 mm ID cat.#

4.0 mm ID cat.#

917433E 917435E 917431E 917436E

ColumnC haracteristics: particle size:

4.6 mm ID cat.# 9174335 9174355 9174315 9174365

CH3

Si

CH3

O 9174531 9174551 9174511 9174561 9174521 9174571

9174532 9174552 9174512 9174562 9174522 9174572

917453E 917455E 917451E 917456E 917452E 917457E

9174514 9174564

9174535 9174555 9174515 9174565 9174525 9174575

3 μm or 5 μm, spherical pore size: 100 Å carbon load: 20% end-cap: yes pH range: 2.5 to 8 temperature limit: 80 °C USP phase code: L1 phase category: C18, octadecylsilane ligand type: monomeric C18

C18

Ultra C8 Columns (USP L7) ChromatographicP roperties Our C8 is a conventional monomeric octylsilane column offering a shorter alkyl chain to provide less hydrophobic retention and improved basic peak shape over a traditional C18 phase. Like our C18, this general-purpose Restek C8 is suitable for a wide range of compounds from acidic through slightly basic. Length 3 μm Columns 30 mm 50 mm 100 mm 150 mm 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

1.0 mm ID cat.#

2.1 mm ID cat.#

3.0 mm ID cat.#

4.0 mm ID cat.#

4.6 mm ID cat.#

9103331 9103351 9103311 9103361

9103332 9103352 9103312 9103362

910333E 910335E 910331E 910336E

9103335 9103355 9103315 9103365

9103531 9103551 9103511 9103561 9103521 9103571

9103532 9103552 9103512 9103562 9103522 9103572

910353E 910355E 910351E 910356E 910352E 910357E

9103535 9103555 9103515 9103565 9103525 9103575

9103514 9103564

ColumnC haracteristics: particle size:

CH3

Si O

CH3

3 μm or 5 μm, spherical pore size: 100 Å carbon load: 12% end-cap: yes pH range: 2.5 to 8 temperature limit: 80 °C USP phase code: L7 phase category: C8, octylsilane ligand type: monomeric C8

C8

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149


LC COLUMNS

Columns for HPLC Ultra C4 Columns (USP L26) Chromatographic Properties Exceptionally stable C4 packing with high bonding coverage and base deactivation. Less retention than C18 or C8 phases.

ColumnC haracteristics: particle size:

3 μm or 5 μm, spherical pore size: 100 Å carbon load: 9% end-cap: yes pH range: 2.5 to 8 temperature limit: 80 °C

CH3

Si

CH3 Length 3 μm Columns 30 mm 50 mm 100 mm 150 mm 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

O

C4

1.0 mm ID cat.#

2.1 mm ID cat.#

3.0 mm ID cat.#

4.6 mm ID cat.#

9102331 9102351 9102311 9102361

9102332 9102352 9102312 9102362

910233E 910235E 910231E 910236E

9102335 9102355 9102315 9102365

9102531 9102551 9102511 9102561 9102521 9102571

9102532 9102552 9102512 9102562 9102522 9102572

910253E 910255E 910251E 910256E 910252E 910257E

9102535 9102555 9102515 9102565 9102525 9102575

Ultra C1 Columns (USP L13) Chromatographic Properties Exceptionally stable C1 phase. Least retentive reversed-phase hydrocarbon packing.

ColumnC haracteristics: particle size:

3 μm or 5 μm, spherical pore size: 100 Å carbon load: 5% pH range: 2.5 to 8 temperature limit: 80 °C

CH3

Si O

C1

CH3 Length 3 μm Columns 30 mm 50 mm 100 mm 150 mm 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

1.0 mm ID cat.#

2.1 mm ID cat.#

www.restek.com/chromatograms

150 www.restek.com

4.6 mm ID cat.#

9101331 9101351 9101311 9101361

9101332 9101352 9101312 9101362

910133E 910135E 910131E 910136E

9101335 9101355 9101315 9101365

9101531 9101551 9101511 9101561 9101521 9101571

9101532 9101552 9101512 9101562 9101522 9101572

910153E 910155E 910151E 910156E 910152E 910157E

9101535 9101555 9101515 9101565 9101525 9101575

Chromatogram Search Tool Search by compound name, synonym, CAS #, or keyword

3.0 mm ID cat.#


LC COLUMNS

Columns for HPLC Ultra Aromax Columns (USP L11) ChromatographicP roperties Ultra Aromax is a unique reversed-phase material that exhibits extreme retention and selectivity for aromatic and/or unsaturated compounds. This column is a great alternative to our Biphenyl phase when increased retention is required, and it’s an excellent choice for gradient LC-MS analyses when conventional columns are not giving adequate retention or selectivity. Length 3 μm Columns 30 mm 50 mm 100 mm 150 mm 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

1.0 mm ID cat.#

2.1 mm ID cat.#

3.0 mm ID cat.#

4.6 mm ID cat.#

9127331 9127351 9127311 9127361

9127332 9127352 9127312 9127362

912733E 912735E 912731E 912736E

9127335 9127355 9127315 9127365

9127531 9127551 9127511 9127561 9127521 9127571

9127532 9127552 9127512 9127562 9127522 9127572

912753E 912755E 912751E 912756E 912752E 912757E

9127535 9127555 9127515 9127565 9127525 9127575

ColumnC haracteristics: particle size:

3 μm or 5 μm, spherical pore size: 100 Å carbon load: 17% end-cap: yes pH range: 2.5 to 8 temperature limit: 80 °C USP phase code: L11 phase category: phenyl ligand type: proprietary phenyl ligand

Proprietary CH3

Si

CH3

O

Aromax

Ultra Aqueous C18 Columns (USP L1) ChromatographicP roperties The Restek Aqueous C18 is a rugged, reversed-phase column with a well-balanced retention profile. It can effectively retain more types of solutes than a conventional C18 and is ideal for multi-component LC-MS analyses. The general-purpose Aqueous C18 boasts high reproducibility and compatibility with many mobile phase conditions—even 100% aqueous and acidic. And when used with a gradient, it eliminates the all-too-common issue of multiple compounds eluting near the column void time. 1.0 mm ID cat.#

2.1 mm ID cat.#

3.0 mm ID cat.#

particle size:

3 μm or 5 μm, spherical pore size: 100 Å carbon load: 15% end-cap: no pH range: 2.5 to 8 temperature limit: 80 °C USP phase code: L1 phase category: modified C18 ligand type: proprietary polar modified and functionally bonded C18

4.6 mm ID cat.#

Si 9178331 9178351 9178311 9178361

9178332 9178352 9178312 9178362

917833E 917835E 917831E 917836E

9178335 9178355 9178315 9178365

9178531 9178551 9178511 9178561 9178521 9178571

9178532 9178552 9178512 9178562 9178522 9178572

917853E 917855E 917851E 917856E 917852E 917857E

9178535 9178555 9178515 9178565 9178525 9178575

P

O O

Aqueous C18 USLC® Column Interaction Profile on rsi

H-B o

iz

xc

ar

ab

ha

Po l

nge

Dis pe

(See page 138 for more information.)

ing nd

Length 3 μm Columns 30 mm 50 mm 100 mm 150 mm 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

ColumnC haracteristics:

ilit

y

C a ti

on

E

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151


LC COLUMNS

Columns for HPLC Ultra Biphenyl Columns (USP L11) ColumnC haracteristics: 3 μm or 5 μm, spherical pore size: 100 Å carbon load: 15% end-cap: yes pH range: 2.5 to 8 temperature limit: 80 °C USP phase: L11 CH3 phase category: phenyl ligand type: unique Biphenyl

Chromatographic Properties

particle size:

Si

The Restek Biphenyl offers a greater degree of dispersion than conventional phenyls and a greater degree of polarizability than phenyl hexyls, creating higher selectivity and a greater range of usability. Because of these heightened interactions, this column shows substantial increases in retention—especially for dipolar, unsaturated, or conjugated solutes—and enhanced orthogonal selectivity when using methanol mobile phases. It is ideal for increasing sensitivity and selectivity in LC-MS analyses.

CH3

Length 3 μm Columns 30 mm 50 mm 100 mm 150 mm 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

Biphenyl

USLC® Column Interaction Profile H-B o

3.0 mm ID cat.#

4.6 mm ID cat.#

9109331 9109351 9109311 9109361

9109332 9109352 9109312 9109362

910933E 910935E 910931E 910936E

9109335 9109355 9109315 9109365

9109531 9109551 9109511 9109561 9109521 9109571

9109532 9109552 9109512 9109562 9109522 9109572

910953E 910955E 910951E 910956E 910952E 910957E

9109535 9109555 9109515 9109565 9109525 9109575

iz

xc

ar

ab

2.1 mm ID cat.#

ha

Po l

nge

ing nd

Dis pe

(See page 138 for more information.) on rsi

1.0 mm ID cat.#

O

ilit

C a ti

y

on

E

Steroid Panel Analysis on the Ultra Biphenyl 15

5

12

16

8

10

7

➲ See page 698

13

for conditions and peak list.

11 9 4

6

4.3 4.4

4.5 4.6 4.7 4.8 4.9 5.0 5.1

5.2 5.3 5.4 5.5 5.6

3

2 1

14 0.0

0.5

1.0

1.5

2.0

2.5

3.0

3.5

4.0

4.5

5.0 Time (min)

152 www.restek.com

5.5

6.0

6.5

7.0

7.5

8.0

8.5

9.0

9.5

LC_CF0537


LC COLUMNS

Columns for HPLC Ultra IBD Columns (USP L68) Column Characteristics:

ChromatographicP roperties

particle size:

The Restek IBD is a polar-embedded column that acts as a strong hydrogen bonder and may be the most versatile column available today. With a unique polar group, this column is very retentive and selective for acids. It also provides symmetrical peak shape for strong bases. Restek’s IBD is compatible with 100% aqueous mobile phases and can be used under reversed-phase or HILIC conditions to retain very polar, ionic compounds in highly organic mobile phases. 1.0 mm ID cat.#

2.1 mm ID cat.#

3.0 mm ID cat.#

4.6 mm ID cat.#

9175331 9175351 9175311 9175361

9175332 9175352 9175312 9175362

917533E 917535E 917531E 917536E

9175335 9175355 9175315 9175365

9175531 9175551 9175511 9175561 9175521 9175571

9175532 9175552 9175512 9175562 9175522 9175572

917553E 917555E 917551E 917556E 917552E 917557E

9175535 9175555 9175515 9175565 9175525 9175575

CH3

Si O

IBD USLC® Column Interaction Profile (See page 138 for more information.) H-B o

iz

xc

ar

ab

ha

Po l

nge

Dis pe

on rsi

ing nd

Length 3 μm Columns 30 mm 50 mm 100 mm 150 mm 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

P

3 μm or 5 μm, spherical pore size: 100 Å carbon load: 12% end-cap: no pH range: 2.5 to 8 temperature limit: 80 °C USP phase code: L68 CH3 phase category: polar-embedded alkyl ligand type: proprietary polar functional embedded alkyl

ilit

y

C a ti

on

E

Ultra PFP Propyl Columns (USP L43) Column Characteristics:

ChromatographicP roperties The Restek PFP Propyl is a great choice for the retention and selectivity of charged bases, electronegative compounds, and amine-containing compounds. Unlike a conventional cyano column, however, the Restek PFP Propyl is much more amenable to LC-MS because it is more reliable and efficient with acidic mobile phases. This versatile column is also compatible with highly aqueous mobile phases and HILIC separations. 1.0 mm ID cat.#

2.1 mm ID cat.#

3.0 mm ID cat.#

4.6 mm ID cat.#

9179331 9179351 9179311 9179361

9179332 9179352 9179312 9179362

917933E 917935E 917931E 917936E

9179335 9179355 9179315 9179365

9179531 9179551 9179511 9179561 9179521 9179571

9179532 9179552 9179512 9179562 9179522 9179572

917953E 917955E 917951E 917956E 917952E 917957E

9179535 9179555 9179515 9179565 9179525 9179575

F F F CH3

Si O

3 μm or 5 μm, spherical pore size: 100 Å F carbon load: 11% end-cap: yes 2.5 to 8 F pH range: temperature limit: 80 °C USP phase code: L43 CH3 phase category: fluorophenyl propyl ligand type: pentafluorophenyl propyl

PFP Propyl USLC® Column Interaction Profile on rsi

H-B o

iz

xc

ar

ab

ha

Po l

nge

Dis pe

(See page 138 for more information.)

ing nd

Length 3 μm Columns 30 mm 50 mm 100 mm 150 mm 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

particle size:

ilit

y

C a ti

on

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LC COLUMNS

Columns for HPLC Ultra Cyano Columns (USP L10) Chromatographic Properties ColumnC haracteristics: 3 μm or 5 μm, spherical pore size: 100 Å CH3 carbon load: 8% end-cap: yes pH range: 2.5 to 8 temperature limit: 80 °C USP phase code: L10 phase category: cyano ligand type: cyanopropylsilane

N

C

particle size:

Si

CH3

O

Cyano

The Restek Cyano is a traditional monomeric cyanopropylsilane that is recommended for assays where alternate selectivity, or confirmation, to a C18 or C8 column is desired. It can be used in normal-phase, reversed-phase (best with mobile phase pH between 5 and 7), and HILIC modes. It is an excellent choice for the analysis of protonated bases. Length 3 μm Columns 30 mm 50 mm 100 mm 150 mm 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

1.0 mm ID cat.#

2.1 mm ID cat.#

3.0 mm ID cat.#

4.6 mm ID cat.#

9106331 9106351 9106311 9106361

9106332 9106352 9106312 9106362

910633E 910635E 910631E 910636E

9106335 9106355 9106315 9106365

9106531 9106551 9106511 9106561 9106521 9106571

9106532 9106552 9106512 9106562 9106522 9106572

910653E 910655E 910651E 910656E 910652E 910657E

9106535 9106555 9106515 9106565 9106525 9106575

Ultra Amino Columns (USP L8) Chromatographic Properties NH 2

ColumnC haracteristics:

3 μm or 5 μm, spherical CH3 Si CH3 pore size: 100 Å carbon load: 2% O end-cap: no pH range: 2.5 to 8 temperature limit: 80 °C USP phase code: L8 phase category: amino ligand type: aminopropylsilane Amino

The general-purpose Restek Amino is an aminopropylsilane that offers reproducible retention and efficiency. It is a great choice for the normal-phase or HILIC analysis of simple sugars.

particle size:

Length 3 μm Columns 30 mm 50 mm 100 mm 150 mm 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

1.0 mm ID cat.#

2.1 mm ID cat.#

3.0 mm ID cat.#

4.6 mm ID cat.#

9107331 9107351 9107311 9107361

9107332 9107352 9107312 9107362

910733E 910735E 910731E 910736E

9107335 9107355 9107315 9107365

9107531 9107551 9107511 9107561 9107521 9107571

9107532 9107552 9107512 9107562 9107522 9107572

910753E 910755E 910751E 910756E 910752E 910757E

9107535 9107555 9107515 9107565 9107525 9107575

Ultra Silica Columns (USP L3) Chromatographic Properties Base-deactivated spherical silica is useful for normal-phase or HILIC separations.

ColumnC haracteristics: particle size:

3 μm or 5 μm, spherical pore size: 100 Å end-cap: no pH range: 2.5 to 8 temperature limit: 80 °C USP phase code: L3 phase category: bare silica ligand type: none

154 www.restek.com

OH Si O O O

Silica

Length 3 μm Columns 30 mm 50 mm 100 mm 150 mm 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

1.0 mm ID cat.#

2.1 mm ID cat.#

3.0 mm ID cat.#

4.6 mm ID cat.#

9100331 9100351 9100311 9100361

9100332 9100352 9100312 9100362

910033E 910035E 910031E 910036E

9100335 9100355 9100315 9100365

9100531 9100551 9100511 9100561 9100521 9100571

9100532 9100552 9100512 9100562 9100522 9100572

910053E 910055E 910051E 910056E 910052E 910057E

9100535 9100555 9100515 9100565 9100525 9100575


LC COLUMNS

Columns for Bioseparations Restekmanufactured silica

Viva HPLC Columns: 3 or 5 μm particles; 300 Å pore size • Excellent for separating peptides or proteins. • Rugged, spherical particles with 300 Å pore size. • High proportion of pore/surface area available to large molecules. Viva columns are based on a wide-pore material we designed for optimal largemolecule separations. In developing Viva silica, we found that although many commercial wide-pore silicas meet the standard 300 Å mean pore size, most have very broad distributions about this mean with a significant portion of their pore volume falling below 150 Å. This means a large portion of the surface area is unavailable to larger molecules. Viva columns have a narrow distribution around the mean pore size, permitting a larger portion of the silica surface to play a role in the separation.

Restekmanufactured silica

Viva C18 Columns (USP L1) ChromatographicP roperties

The general-purpose Restek C18 is a conventional monomeric octadecylsilane column suitable for analyses of a wide range of compounds from acidic through slightly basic. Length 3 μm Columns 30 mm 50 mm 100 mm 150 mm 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

1.0 mm ID cat.# 9514331 9514351 9514311 9514361

2.1 mm ID cat.# 9514332 9514352 9514312 9514362

3.0 mm ID cat.# 951433E 951435E 951431E 951436E

ColumnC haracteristics: particle size:

4.6 mm ID cat.# 9514335 9514355 9514315 9514365

CH3

Si

CH3

O 9514531 9514551 9514511 9514561 9514521 9514571

9514532 9514552 9514512 9514562 9514522 9514572

951453E 951455E 951451E 951456E 951452E 951457E

9514535 9514555 9514515 9514565 9514525 9514575

3 μm or 5 μm, spherical pore size: 300 Å carbon load: 9% end-cap: yes pH range: 2.5 to 8 temperature limit: 80 °C USP phase code: L1 phase category: C18, octadecylsilane ligand type: monomeric C18

C18

Restekmanufactured silica

Viva C8 Columns (USP L7) ChromatographicP roperties

Our C8 is a conventional monomeric octylsilane column offering a shorter alkyl chain to provide less hydrophobic retention and improved basic peak shape over a traditional C18 phase. Like our C18, this general-purpose Restek C8 is suitable for a wide range of compounds from acidic through slightly basic. Length 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

1.0 mm ID cat.# 9513531 9513551 9513511 9513561 9513521 9513571

2.1 mm ID cat.# 9513532 9513552 9513512 9513562 9513522 9513572

3.0 mm ID cat.# 951353E 951355E 951351E 951356E 951352E 951357E

4.6 mm ID cat.# 9513535 9513555 9513515 9513565 9513525 9513575

ColumnC haracteristics: CH3

Si O

CH3

particle size: pore size: carbon load: end-cap: pH range: temperature limit: USP phase code: phase category: ligand type:

5 μm, spherical 300 Å 5% yes 2.5 to 8 80 °C L7 C8, octylsilane monomeric C8

C8

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LC COLUMNS

Columns for Bioseparations Restekmanufactured silica

Viva C4 Columns (USP L26) Chromatographic Properties

Base-deactivated, wide-pore packing exhibits excellent peak shape for a wide range of compounds. Less retention in reversed-phase assays than Viva C18 or Viva C8.

ColumnC haracteristics: particle size: pore size: carbon load: end-cap: pH range: temperature limit:

5 μm, spherical 300 Å 3.5% yes 2.5 to 8 80 °C

CH3

Si

CH3

O

C4

Length 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

1.0 mm ID cat.# 9512531 9512551 9512511 9512561 9512521 9512571

2.1 mm ID cat.# 9512532 9512552 9512512 9512562 9512522 9512572

3.0 mm ID cat.# 951253E 951255E 951251E 951256E 951252E 951257E

4.6 mm ID cat.# 9512535 9512555 9512515 9512565 9512525 9512575

Restekmanufactured silica

Viva Biphenyl Columns (USP L11) Chromatographic Properties

The Restek Biphenyl offers a greater degree of dispersion than conventional phenyls and a greater degree of polarizability than phenyl hexyls, creating higher selectivity and a greater range of usability. Because of these heightened interactions, this column shows substantial increases in retention—especially for dipolar, unsaturated, or conjugated solutes—and enhanced orthogonal selectivity when using methanol mobile phases. It is ideal for increasing sensitivity and selectivity in LC-MS analyses.

ColumnC haracteristics: particle size: pore size: carbon load: end-cap: pH range: temperature limit: USP phase: phase category: ligand type:

5 μm, spherical 300 Å 7% yes 2.5 to 8 80 °C L11 CH3 phenyl unique Biphenyl

Si

CH3

O

Biphenyl

Length 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

1.0 mm ID cat.# 9516531 9516551 9516511 9516561 9516521 9516571

2.1 mm ID cat.# 9516532 9516552 9516512 9516562 9516522 9516572

3.0 mm ID cat.# 951653E 951655E 951651E 951656E 951652E 951657E

4.6 mm ID cat.# 9516535 9516555 9516515 9516565 9516525 9516575

Restekmanufactured silica

Viva PFP Propyl Columns (USP L43) Chromatographic Properties ColumnC haracteristics: particle size: pore size: carbon load: end-cap: pH range: temperature limit: USP phase code: phase category: ligand type:

5 μm, spherical 300 Å F 5% yes F 2.5 to 8 80 °C CH3 L43 fluorophenyl propyl pentafluorophenyl propyl

PFP Propyl

156 www.restek.com

F F F Si O

CH3

The Restek PFP Propyl is a great choice for the retention and selectivity of charged bases, electronegative compounds, and amine-containing compounds. Unlike a conventional cyano column, however, the Restek PFP Propyl is much more amenable to LC-MS because it is more reliable and efficient with acidic mobile phases. This versatile column is also compatible with highly aqueous mobile phases and HILIC separations. Length 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

1.0 mm ID cat.# 9519531 9519551 9519511 9519561 9519521 9519571

2.1 mm ID cat.# 9519532 9519552 9519512 9519562 9519522 9519572

3.0 mm ID cat.# 951953E 951955E 951951E 951956E 951952E 951957E

4.6 mm ID cat.# 9519535 9519555 9519515 9519565 9519525 9519575


LC COLUMNS

Columns for Bioseparations Restekmanufactured silica

Viva Silica Columns (USP L3) ChromatographicP roperties

Base-deactivated spherical silica is useful for normal-phase or HILIC separations. Length 5 μm Columns 30 mm 50 mm 100 mm 150 mm 200 mm 250 mm

1.0 mm ID cat.#

2.1 mm ID cat.#

3.0 mm ID cat.#

OH

4.6 mm ID cat.#

Si O O O

9510531 9510551 9510511 9510561 9510521 9510571

9510532 9510552 9510512 9510562 9510522 9510572

951053E 951055E 951051E 951056E 951052E 951057E

9510535 9510555 9510515 9510565 9510525 9510575

ColumnC haracteristics: particle size: pore size: pH range: temperature limit: USP phase code: phase category: ligand type:

5 μm, spherical 300 Å 2.5 to 8 80 °C L3 bare silica none

Silica

Choose Cost-Effective Restek LC Kits For Your Preventative Maintenance • Significant savings over instrument manufacturer prices. • High-quality components in every kit.

25270

• Wide range of options for LC systems and pumps. See page 299 for Agilentsystem kits or page 307 for Waters-system kits.

25145

www.restek.com/LC-Maintenance

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LC COLUMNS

Application-Specific Columns Application-Specific LC Phases Restekmanufactured silica

Pinnacle® II PAH HPLC Columns ChromatographicP roperties ColumnC haracteristics: particle size: pore size: end-cap: pH range: temperature limit:

4 μm, spherical 110 Å no 2.5 to 8 80 °C

Developed specifically for challenging analyses of polycyclic aromatic hydrocarbons (PAHs). The Pinnacle® II PAH stationary phase incorporates a proprietary C18 bonding that enables unique shape selectivity to baseline resolve all 16 PAHs listed in U.S. EPA Method 610 plus two other routinely analyzed PAH compounds. Every lot of Pinnacle® II PAH bonded phase material is tested to ensure baseline resolution of the Method 610 PAHs using a simple water/ acetonitrile mobile phase gradient. Further, because we make Pinnacle® II PAH columns using our own silica, we have greater control over quality and reproducibility. If you are analyzing PAHs using HPLC, Pinnacle® II PAH columns are a reliable, cost-effective choice. Length 4 μm Columns 50 mm 100 mm 150 mm 200 mm 250 mm

2.1 mm ID cat.#

3.0 mm ID cat.#

9219452 9219412 9219462 9219422 9219472

4.6 mm ID cat.#

921945E 921941E 921946E 921942E 921947E

9219455 9219415 9219465 9219425 9219475

Pinnacle® II PAH Guard Cartridges Guard Cartridges Pinnacle II PAH Guard Cartridge

3-pk. (10 x 2.1 mm) 921950212

3-pk. (10 x 4.0 mm) 921950210

How can we help you today? Contact us... customer service: 1-814-353-1300, ext. 3 | e-mail: csreps@restek.com technical service: 1-814-353-1300, ext. 4 | e-mail: support@restek.com sales representatives: 1-814-353-1300, ext. 3 | e-mail: salesreps@restek.com 4 ways to order: 1. CALL: 1-800-356-1688, ext. 3 or 1-814-353-1300, ext. 3 Monday–Friday 8:00 a.m.–6:30 p.m. Eastern Time 2. FAX: 1-814-353-1309 24 hours a day 3. ONLINE: www.restek.com 24 hours a day 4. E-MAIL: csreps@restek.com 24 hours a day Outside the U.S.? Find a distributor at

www.restek.com/distributor

158 www.restek.com


LC COLUMNS

Application-Specific Columns Restekmanufactured silica

Pinnacle® DB PAH UHPLC Columns ChromatographicP roperties

Specifically designed to resolve complex mixtures of polycyclic aromatic hydrocarbons (PAHs). Get complete resolution of all 16 EPA 610 PAHs plus two other routinely analyzed PAH compounds in less than five minutes to greatly reduce run times and increase sample throughput.

ColumnC haracteristics: particle size: pore size: end-cap: pH range: temperature limit:

2.1 mm ID cat.#

Length 1.9 μm Columns 30 mm 50 mm 100 mm

1.9 μm, spherical 140 Å no 2.5 to 8 80 °C

9470232 9470252 9470212

PAH Mix on Pinnacle® DB PAH 8

12

7 11

0.00

0.50

1.00

15

9 10 2

1.50

Column Dimensions: Particle Size: Pore Size: Temp.: Sample Diluent: Conc.: Inj. Vol.: Mobile Phase A: B:

Max Pressure: Detector Instrument

16

3 4 5

2.00

18

14

6 1

13

2.50 Time (min)

3.00

3.50

4.00

17

4.50

5.00 LC_EV0521

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18.

Peaks Naphthalene Acenaphthylene 1-Methylnaphthalene 2-Methylnaphthalene Acenaphthene Fluorene Phenanthrene Anthracene Fluoranthene Pyrene Benzo[a]anthracene Chrysene Benzo[b]fluoranthene Benzo[k]fluoranthene Benzo[a]pyrene Dibenzo[a,h]anthracene Benzo[ghi]perylene Indeno[1,2,3-cd]pyrene

Pinnacle® DB PAH (cat.# 9470252) 50 mm x 2.1 mm ID 1.9 μm 140 Å 30 °C EPA Method 8310 PAH Mixture (cat.# 31841) acetonitrile 10 μg/mL 1 μL water acetonitrile Time (min) Flow (mL/min) 0 0.8 2 0.8 4 0.8 4.5 0.8 4.51 0.8 5 0.8 724 bar Photo diode array @ 254, 4.8 nm Waters

%A 60 40 0 0 60 60

%B 40 60 100 100 40 40

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LC COLUMNS

Application-Specific Columns Allure® AK Columns Chromatographic Properties This highly retentive, highly selective phase—unique to Restek—was developed specifically for the analysis of aldehydes and ketones as DNPH derivatives. Allure® AK is a reversed-phase HPLC material that has the unique ability to separate all 13 carbonyl compounds specified in California Air Resources Board (CARB) Method #1004, using a simple acetonitrile/water gradient. Other columns require long analysis times or the use of tetrahydrofuran.

ColumnC haracteristics: particle size: pore size: end-cap: pH range: temperature limit:

5 μm, spherical 60 Å yes 2.5 to 8 80 °C

3.2 mm ID cat.#

Length 5 μm Columns with Trident Integral Inlet Fittings 200 mm

4.6 mm ID cat.#

9159523-700

9159525-700

Allure® AK Guard Cartridge 3-pk. (10 x 4.0 mm) 915950210

Guard Cartridges Allure AK Guard Cartridge

Carbonyls by CARB Method 1004 on Allure® AK

1

Peaks RT (min) 1. Formaldehyde 4.74 2. Acetaldehyde 5.78 3. Acrolein 6.86 4. Acetone 7.09 5. Propionaldehyde 7.31 6. Crotonaldehyde 8.19 7. Methacrolein 8.55 8. Butyraldehyde 8.79 9. Methyl ethyl ketone 9.06 10. Benzaldehyde 10.03 11. Valeraldehyde 10.39 12. m-Tolualdehyde 11.08 13. hexanaldehyde 11.36 DNPH derivatives of aldehydes and ketones

2 3 13

4 5

7 11

6 8

12

9 10

1.0

2.0

3.0

4.0

Column Dimensions: Particle Size: Pore Size: Temp.: Sample Diluent: Conc.: Inj. Vol.: Mobile Phase A: B:

Flow: Detector

160 www.restek.com

5.0

6.0

7.0

8.0

9.0

Allure® AK (cat.# 9159525-700) 200 mm x 4.6 mm ID 5 μm 60 Å 30 °C dinitrophenylhydrazine (DNPH) derivatives of aldehydes/ketones acetonitrile 3 μg/mL each analyte, as aldehyde/ketone 10 μL water acetonitrile Time (min) %B 0 60 8 70 10 100 1.5 mL/min UV/Vis @ 360 nm

10.0

11.0 Time (min) LC_EV0393


LC COLUMNS

Application-Specific Columns Allure® Organic Acids Columns ChromatographicP roperties Allure® Organic Acids columns provide enhanced retention and selectivity for polar organic acids, allowing the separation to be performed on a single 30 cm column. An Allure® Organic Acids column effectively resolves key organic acids such as tartaric and quinic acids using the chromatographic conditions specified in AOAC method 986.13. Retention is stable and reproducible, even with the 100% aqueous mobile phase specified in the AOAC method. 3.0 mm ID cat.#

Length 5 μm Column 150 mm 250 mm 300 mm

4.6 mm ID cat.#

916556E

ColumnC haracteristics: particle size: pore size: end-cap: pH range: temperature limit:

5 μm, spherical 60 Å no 2.5 to 8 80 °C

9165565 9165575 9165585

Allure® Organic Acids Guard Cartridges 3-pk. (10 x 2.1 mm) 916550212

Guard Cartridges Allure Organic Acids Guard Cartridge

3-pk. (10 x 4.0 mm) 916550210

Organic Acids on Allure® Organic Acids 1

1. 2. 3. 4. 5.

Peaks Tartaric acid Quinic acid Malic acid Citric Acid Fumaric Acid

Conc. (mg/mL) 1.0 1.0 1.0 1.0 0.005

2 3

4

5

0

5

10

15

20

25

30

35

40

Time (min)

LC_0238 Column Dimensions: Particle Size: Pore Size: Temp.: Sample Diluent: Inj. Vol.: Mobile Phase Flow: Detector

Allure® Organic Acids (cat.# 9165585) 300 mm x 4.6 mm ID 5 μm 60 Å ambient standard solution water 10.0 μL 100 mM phosphate buffer, pH 2.5 0.5 mL/min UV/Vis @ 226 nm

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161


LC COLUMNS

Application-Specific Columns Ultra Carbamate Columns Chromatographic Properties Restek chemists developed the Ultra Carbamate column specifically for carbamates analysis. The unique packing separates 10 target carbamates in just over 10 minutes. The column is compatible with fluorescence or LC-MS detection.* An Ultra Carbamate column can process as many as three samples per hour, versus less than two samples per hour on a general-purpose C18 column. In addition to increased sample throughput, this much faster analysis will significantly reduce solvent usage—and the costs of disposing of solvent waste.

ColumnC haracteristics: particle size: pore size: pH range: temperature limit:

3 μm or 5 μm, spherical 100 Å 2.5 to 8 80 °C

Length 3 μm Columns 50 mm 100 mm 5 μm Columns 250 mm

1.0 mm ID cat.# 9177351 9177311

2.1 mm ID cat.#

3.0 mm ID cat.#

9177352 9177312

917735E 917731E

4.0 mm ID cat.# 9177354

4.6 mm ID cat.# 9177355 9177315 9177575

*For post-column derivatization/fluorescence detection applications using a 4.6 mm ID column, the total system dead volume, including the post-column reactor, must be less than 650 μL. For standard post-column reactor systems, we recommend a 250 mm x 4.6 mm, 5 μm column. Contact Restek Technical Service or your local Restek representative for more information.

Ultra Carbamate Guard Cartridges 3-pk. (10 x 2.1 mm) 917750212

Guard Cartridges Ultra Carbamate Guard Cartridge

3-pk. (10 x 4.0 mm) 917750210

Carbamates on Ultra Carbamate 8

10

11

7

9

Column Dimensions: Particle Size: Pore Size: Temp.: Sample Diluent: Conc.: Inj. Vol.: Mobile Phase A: B:

Ultra Carbamate (cat.# 9177352) 50 mm x 2.1 mm ID 3 μm 100 Å ambient 531.1 Carbamate Pesticide Calibration Mixture (cat.# 32273) 4-bromo-3,5-dimethylphenyl-N-methylcarbamate (BDMC) (cat.# 32274) methanol 50 μg/mL 1 μL 2 mM ammonium acetate:methanol (v/v, 90/10) 2 mM ammonium acetate:methanol (v/v, 10/90)

6 5 1 2

4 3

0

2.5

5.0

7.5

1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11.

162 www.restek.com

10.0

12.5

15.0

Peaks Aldicarb sulfone Aldicarb sulfoxide Oxamyl Methomyl 3-Hydroxycarbofuran Aldicarb Propoxur Carbofurane Carbaryl Methiocarb BDMC (IS)

17.5

20.0 Time (min) LC_FF0473

Time (min) %A %B 0.00 80 20 20 0 100 25 0 100 Flow: 0.2 mL/min Detector LECO Unique® TOFMS Run Length: 25 min Ionization Source Type: high flow ESI Ion Mode: positive Desolvation Temp.: 130 °C Nebulizing Pressure: 100 kPa Desolvation Gas (N2): 4 L/min Interface Temp.: 120 °C Nozzle Voltage: 62 V Capillary Voltage: 2.75 kV Instrument Agilent 1100 Acknowledgement LECO Corporation


LC COLUMNS

Application-Specific Columns Ultra Quat Columns ChromatographicP roperties A retentive, high-purity, base-deactivated, reversed-phase packing. Ideal for the analysis of paraquat and diquat or other quaternary amines. 2.1 mm ID cat.#

Length 3 μm Column 50 mm 5 μm Column 150 mm

Column Characteristics: particle size: pore size: pH range: temperature limit:

4.6 mm ID cat.#

3 μm or 5 μm, spherical 100 Å 2.5 to 8 80 °C

9181352 9181565

Ultra Quat Guard Cartridges Guard Cartridges Ultra Quat Guard Cartridge

3-pk. (10 x 2.1 mm) 918150212

3-pk. (10 x 4.0 mm) 918150210

Paraquat and Diquat on Ultra Quat

Fast, sensitive LC-MS/MS analysis! Peaks

Precursor Ion (amu) 1. Diquat 183+ 2. Paraquat 93 (2+)

Fragment Ion (amu) 157+ 171+

DP (V) 30 20

Collision Energy (eV) 30 20

LC_EV0383 Column Dimensions: Particle Size: Pore Size: Temp.: Sample Diluent: Conc.: Inj. Vol.: Mobile Phase Flow: Detector Model #: Ion Source: Ion Mode: Ion Spray Voltage: Curtain Gas: Gas 1: Gas 2: Source Temp.: Mode: Dwell Time: Instrument Notes

Ultra Quat (cat.# 9181352) 50 mm x 2.1 mm ID 3 μm 100 Å ambient

DI Water 5 μg/mL each component 10 μL 10 mM heptafluorobutyric acid:acetonitrile (95:5) 0.3 mL/min Applied Biosystems/MDS Sciex LC-MS/MS API 3200™ MS/MS system Electrospray ESI+ 5.5 kV 15 psi (103.4 kPa) 70 psi (482.6 kPa) 60 psi (413.7 kPa) 600 °C MRM 200 ms Applied Biosystems/MDS Sciex LC-MS/MS System Collision exit potential: 3V Q1/Q3: unit resolution Acknowledgement Data courtesy of Houssain El Aribi, Ph.D., LC/MS Product and Application Specialist, MDS SCIEX, 71 Four Valley Drive, Concord, Ontario, Canada, L4K 4V8

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LC COLUMNS

Prep Columns Ultra Preparative Columns

Column Characteristics: particle size: pore size: carbon load: end-cap: pH range: temperature limit: USP phase code: phase category: ligand type:

Using Restek Ultra columns for preparative applications can save you time, solvents, and money. By utilizing the right phase for your prep analysis, you can make sure your peaks are resolved and your compounds are pure. The Ultra line has high loading and features high-purity silica.

5 μm, spherical 100 Å 15% no 2.5 to 8 80 °C L1 modified C18 proprietary polar modified and functionally bonded C18

P

Si O O

Ultra Aqueous C18 HPLC Prep Columns ChromatographicP roperties

Aqueous C18 USLC® Column Interaction Profile H-B o

ing nd

iz

xc

ar

ha

Po l ab

ilit

y

o C a ti

The Restek Aqueous C18 is a rugged, reversed-phase column with a well-balanced retention profile. It can effectively retain more types of solutes than a conventional C18 and is ideal for multi-component LC-MS analyses. The general-purpose Aqueous C18 boasts high reproducibility and compatibility with many mobile phase conditions—even 100% aqueous and acidic. And when used with a gradient, it eliminates the all-too-common issue of multiple compounds eluting near the column void time. Length 5 μm Columns 50 mm 100 mm 150 mm 250 mm

nge

Dis pe

(See page 138 for more information.) on rsi

USLC® Phases for Preparative HPLC

nE

10 mm ID cat.# 9178557 9178517 9178567 9178577

21.2 mm ID cat.# 9178558 9178518 9178568 9178578

30 mm ID cat.# 9178559 9178519 9178569 9178579

Ultra IBD HPLC Prep Columns Column Characteristics: particle size: pore size: carbon load: end-cap: pH range: temperature limit: USP phase code: phase category:

5 μm, spherical 100 Å 12% no 2.5 to 8 80 °C L68 polar-embedded CH3 alkyl proprietary polar functional embedded alkyl

ligand type:

ChromatographicP roperties The Restek IBD is a polar-embedded column that acts as a strong hydrogen bonder and may be the most versatile column available today. With a unique polar group, this column is very retentive and selective for acids. It also provides symmetrical peak shape for strong bases. Restek’s IBD is compatible with 100% aqueous mobile phases and can be used under reversed-phase or HILIC conditions to retain very polar, ionic compounds in highly organic mobile phases.

P Si

CH3

O

IBD USLC® Column Interaction Profile H-B o

iz

xc

ar

ab

ha

Po l

nge

on rsi

ing nd

Dis pe

(See page 138 for more information.)

ilit

y

C a ti

on

164 www.restek.com

E

Length 5 μm Columns 50 mm 100 mm 150 mm 250 mm

10 mm ID cat.# 9175557 9175517 9175567 9175577

21.2 mm ID cat.# 9175558 9175518 9175568 9175578

30 mm ID cat.# 9175559 9175519 9175569 9175579


LC COLUMNS

Prep Columns Ultra Biphenyl Prep Columns Column Characteristics:

ChromatographicP roperties The Restek Biphenyl offers a greater degree of dispersion than conventional phenyls and a greater degree of polarizability than phenyl hexyls, creating higher selectivity and a greater range of usability. Because of these heightened interactions, this column shows substantial increases in retention—especially for dipolar, unsaturated, or conjugated solutes—and enhanced orthogonal selectivity when using methanol mobile phases. It is ideal for increasing sensitivity and selectivity in LC-MS analyses. Length 5μ mCo lumns 50 mm 100 mm 150 mm 250 mm

10 mm ID cat.# 9109557 9109517 9109567 9109577

21.2 mm ID cat.# 9109558 9109518 9109568 9109578

CH3

30 mm ID cat.# 9109559 9109519 9109569 9109579

Si O

particle size: pore size: carbon load: end-cap: pH range: temperature limit: USP phase: CH3 phase category: ligand type:

5 μm, spherical 100 Å 15% yes 2.5 to 8 80 °C L11 phenyl unique Biphenyl

Biphenyl USLC® Column Interaction Profile H-B o

iz

xc

ar

ab

ha

Po l

nge

on rsi

ing nd

Dis pe

(See page 138 for more information.)

ilit

y

C a ti

E

on

Ultra PFP Propyl Prep Columns ChromatographicP roperties The Restek PFP Propyl is a great choice for the retention and selectivity of charged bases, electronegative compounds, and amine-containing compounds. Unlike a conventional cyano column, however, the Restek PFP Propyl is much more amenable to LC-MS because it is more reliable and efficient with acidic mobile phases. This versatile column is also compatible with highly aqueous mobile phases and HILIC separations.

F

Column Characteristics:

Si

particle size: 5 μm, spherical pore size: 100 Å 11% F carbon load: end-cap: yes pH range: 2.5 to 8 CH3 temperature limit: 80 °C USP phase code: L43 phase category: fluorophenyl propyl ligand type: pentafluorophenyl propyl

F

F F CH3

O

9179557 9179517 9179567 9179577

21.2 mm ID cat.# 9179558 9179518 9179568 9179578

30 mm ID cat.# 9179559 9179519 9179569 9179579

PFP Propyl USLC® Column Interaction Profile (See page 138 for more information.) H-B o

nge

iz

xc

ar

ab

ha

Po l

Before You Buy a Prep Column...

on rsi

ing nd

Dis pe

Length 5 μm Columns 50 mm 100 mm 150 mm 250 mm

10 mm ID cat.#

ilit

y

C a ti

on

E

PLEASE NOTE: We strongly recommend ordering a semi-prep or prep column only after evaluating the desired separation on an equivalent analyticalscale column. Because we cannot re-use a column or the silica it contains once it has left our facility, we cannot accept returns of large-scale columns.

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165


LC COLUMNS

Prep Columns Traditional Phases for Preparative HPLC

Ultra C18 HPLC Prep Columns ColumnC haracteristics: particle size: pore size: carbon load: end-cap: pH range: temperature limit: USP phase code: phase category: ligand type:

5 μm, spherical 100 Å 20% yes 2.5 to 8 80 °C L1 CH3 C18, octadecylsilane monomeric C18

Chromatographic Properties The general-purpose Restek C18 is a conventional monomeric octadecylsilane column suitable for analyses of a wide range of compounds from acidic through slightly basic.

Si

CH3

O

C18

Length 5 μm Columns 50 mm 100 mm 150 mm 250 mm

10 mm ID cat.# 9174557 9174517 9174567 9174577

21.2 mm ID cat.# 9174558 9174518 9174568 9174578

30 mm ID cat.# 9174559 9174519 9174569 9174579

Ultra C8 HPLC Prep Columns Chromatographic Properties ColumnC haracteristics: particle size: pore size: carbon load: end-cap: pH range: temperature limit: USP phase code: phase category: ligand type:

5 μm, spherical 100 Å 12% yes 2.5 to 8 80 °C L7 C8, octylsilane monomeric C8

CH3

Si

CH3

Our C8 is a conventional monomeric octylsilane column offering a shorter alkyl chain to provide less hydrophobic retention and improved basic peak shape over a traditional C18 phase. Like our C18, this general-purpose Restek C8 is suitable for a wide range of compounds from acidic through slightly basic.

O

C8

Length 5 μm Columns 50 mm 100 mm 150 mm 250 mm

10 mm ID cat.# 9103557 9103517 9103567 9103577

21.2 mm ID cat.# 9103558 9103518 9103568 9103578

30 mm ID cat.# 9103559 9103519 9103569 9103579

Before You Buy a Prep Column... PLEASE NOTE: We strongly recommend ordering a semi-prep or prep column only after evaluating the desired separation on an equivalent analytical-scale column. Because we cannot re-use a column or the silica it contains once it has left our facility, we cannot accept returns of large-scale columns.

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LC COLUMNS

Prep Columns, Bulk Packing Materials Ultra Cyano HPLC Prep Columns ChromatographicP roperties The Restek Cyano is a traditional monomeric cyanopropylsilane that is recommended for assays where alternate selectivity, or confirmation, to a C18 or C8 column is desired. It can be used in normal-phase, reversed-phase (best with mobile phase pH between 5 and 7), and HILIC modes. It is an excellent choice for the analysis of protonated bases. 10 mm ID cat.#

Length 5 μm Columns 50 mm 100 mm 150 mm 250 mm

9106557 9106517 9106567 9106577

21.2 mm ID cat.#

30 mm ID cat.#

9106558 9106518 9106568 9106578

9106559 9106519 9106569 9106579

N

C CH3

Si

CH3

O

ColumnC haracteristics: particle size: pore size: carbon load: end-cap: pH range: temperature limit: USP phase code: phase category: ligand type:

5 μm, spherical 100 Å 8% yes 2.5 to 8 80 °C L10 cyano cyanopropylsilane

Cyano

Ultra Silica HPLC Prep Columns OH

ChromatographicP roperties Base-deactivated spherical silica is useful for normal-phase or HILIC separations. 10 mm ID cat.#

Length 5 μm Columns 50 mm 100 mm 150 mm 250 mm

9100557 9100517 9100567 9100577

21.2 mm ID cat.#

Si

30 mm ID cat.#

9100558 9100518 9100568 9100578

9100559 9100519 9100569 9100579

O O O

ColumnC haracteristics: particle size: pore size: end-cap: pH range: temperature limit: USP phase code: phase category: ligand type:

5 μm, spherical 100 Å no 2.5 to 8 80 °C L3 bare silica none

Silica

Ultra Bulk Packing Materials (5 μm) Use our bulk packing materials to pack your own columns!

• Prepare your own columns in conventional or custom dimensions. • Consistent, high-quality materials. Our extensive QC program ensures the high quality and reproducibility of these silicas. You can be confident that you are getting consistent, high-quality product when you source your silica from Restek. Use these materials for easy scale-up to preparative chromatography or for packing your own columns. Description 5 μm Ultra Bulk Packing Materials Ultra C1 Bulk Packing Ultra C4 Bulk Packing Ultra C8 Bulk Packing Ultra C18 Bulk Packing Ultra Amino Bulk Packing Ultra Cyano Bulk Packing Ultra Silica Bulk Packing

qty.

cat.#

10 g/btl. 10 g/btl. 10 g/btl. 10 g/btl. 10 g/btl. 10 g/btl. 10 g/btl.

91015 91025 91035 91745 91075 91065 91005

1-9 bottles

10-49 bottles 50-99 bottles ≥100 bottles $398/btl. $398/btl. $398/btl. $398/btl. $398/btl. $398/btl. $398/btl.

$373/btl. $373/btl. $373/btl. $373/btl. $373/btl. $373/btl. $373/btl.

$352/btl. $352/btl. $352/btl. $352/btl. $352/btl. $352/btl. $352/btl.

also available Other stationary phases and particle sizes are also available in bulk; call Customer Service or your local Restek representative for details.

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167


LC COLUMNS

UHPLC Filters

Protect Your Column and Your UHPLC Performance With UltraShield and UltraLine UHPLC Filters A cost-effective way to extend the lifetime of any UHPLC column without sacrificing your UHPLC performance on any LC system

Use with any UHPLC system

24995

24993

UltraShield UHPLC PreColumn Filter

UltraLine UHPLC In-Line Filter

• • • • •

• • • • •

Cost-effective protection for UHPLC systems. Reliable way to extend column lifetime. Universal fit—connects easily to any brand column. Leak-tight to 15,000 psi (1,034 bar). 0.5 μm titanium filter in stainless steel body with PEEK ferrule.

Specifications: Inlet/Outlet: Port Geometry: Material: Filter: Pressure Rating: Wrench Flat:

Specifications: Inlet/Outlet: Port Geometry: Material: Filter: Pressure Rating: Wrench Flat:

Female/Male 10-32 Parker (1/16 CPI) Stainless steel, PEEK ferrule 0.5 μm titanium 15,000 psig (1,034 bar) 5/16"

Description UltraShield UHPLC PreColumn Filter UltraShield UHPLC PreColumn Filter UltraShield UHPLC PreColumn Filter

168 www.restek.com

In-line design installs easily with standard fittings. Cost-effective protection for UHPLC systems. Reliable way to extend column lifetime. Leak-tight to 15,000 psi (1,034 bar). Replaceable 0.5 μm stainless steel filter in stainless steel body.

qty. ea. 5-pk. 10-pk.

cat.# 24995 24996 24997

Female/Female 10-32 Parker (1/16 CPI) Stainless steel housing 0.5 μm stainless steel, 0.125" W x 0.062" T, 5 μL volume 15,000 psig (1,034 bar) 3/8"

Description UltraLine UHPLC In-Line Filter (In-Line Assembly with Filter) UltraLine Replacement Filters

qty.

cat.#

ea.

24993

5-pk.

24994


LC COLUMNS

HPLC Guard Cartridge Systems Trident Direct Guard Cartridge System Easy to Use, Low Dead Volume— The Ultimate Combination of Convenience and Column Protection

Unlike “one size fits all” guard systems, the Trident direct system gives you the power to select the right level of protection for your analysis. The system offers three levels of protection and guard cartridges in four dimensions, with a variety of bonded phases to match your analytical column. The economical, leak-free cartridge design provides an unprecedented combination of convenience, economy, and reliability. The foundation of the Trident direct system is a reusable direct connect holder that easily attaches to any HPLC column using CPI- or Waters-style end fittings.* The system is available in configurations to match different protection levels: in-line filter, in-line filter with holder for 10 mm guard cartridge. The guard cartridges are available in 2.1 and 4.0 mm ID and are interchangeable within the appropriate length holder.

Trident direct 10 mm guard cartridge holder with filter Components XF XG-XF Guard PEEK Fitting Fitting Cartridge Tip

Cap Frit

Assembled

25082 Protection against particulate matter.

25084 Protection against particulate matter and moderate protection against irreversibly adsorbed compounds.

Description High-pressure filter 10 mm guard cartridge holder without filter 10 mm guard cartridge holder with filter PEEK tip for Waters-style end fittings PEEK tip for standard fittings

qty. ea. ea. ea. ea. ea.

Installed onto column

cat.# 25082 25083 25084 25088 25087

*The standard PEEK tip in Trident direct systems is compatible with Parker, Upchurch Scientific, Valco, and other CPI-style fittings. To use Trident direct systems with Waters-style end fittings, replace the tip with cat.# 25088.

Double the Protection With Cap Frits! Replacement Cap Frits Replacement guard cartridges can cost as much as an analytical column, so why not protect them, too? The removable cap frit in a Trident direct helps prevent clogged cartridges to extend the life of your column, your cartridge, and your budget! Description Replacement Cap Frits Replacement Cap Frits Replacement Cap Frits Replacement Cap Frits

ID 4 mm 4 mm 2 mm 2 mm

Porosity 2.0 μm 0.5 μm 2.0 μm 0.5 μm

qty. 5-pk. 5-pk. 5-pk. 5-pk.

cat.# 25022 25023 25057 25990

25022

www.restek.com

169


LC COLUMNS

HPLC Guard Cartridge Systems Trident HPLC In-Line Guard Cartridge Holders

Trident in-line 10 mm guard cartridge holder with filter Components XG-XF Fitting

XF Fitting

Guard Cartridge

A Trident in-line guard cartridge holder can be used with almost any HPLC column by connecting it with a short piece of 1/16" tubing, appropriate nuts and ferrules, or fingertight fittings. The system can be used with Restek columns, or with columns from other manufacturers. Holders are available for either 10 mm or 20 mm guard cartridges. Either size can be purchased with or without a prefilter, which provides added protection against the particles that can shorten the lifetime of the guard cartridge.

Body

Cap Frit

Assembled 25021

Installed onto column

25040

Description Holder for 10 mm guard cartridge Holder with filter for 10 mm guard cartridge Frit-Type In-Line Filter, 2.0 μm

For HPLC tubing, visit

NEW!

qty. ea. ea. ea.

www.restek.com/lcacc

EXP® Reusable Fittings for HPLC & UHPLC for 10-32 fittings and 1/16" tubing EXP® Hand-Tight Coupler Description EXP Hand-Tight Coupler (2 Nuts, 2 Ferrules, 1/16" x 0.005" ID Tubing)

25940

cat.# 25021 25040 25041

qty. ea.

cat.# 25940

Hybrid Ferrule U.S. Patent No. 8201854, Optimize Technologies. Optimize Technologies EXP Holders are Patent Pending. Other U.S. and Foreign Patents Pending. The Opti- prefix is a registered trademark of Optimize Technologies, Inc.

also available Our entire EXP® fitting selection. See page 313.

Double the Protection With Cap Frits! Replacement Cap Frits Replacement guard cartridges can cost as much as an analytical column, so why not protect them, too? The removable cap frit in a Trident direct helps prevent clogged cartridges to extend the life of your column, your cartridge, and your budget!

25057

170 www.restek.com

Description Replacement Cap Frits Replacement Cap Frits Replacement Cap Frits Replacement Cap Frits

ID 4 mm 4 mm 2 mm 2 mm

Porosity 2.0 μm 0.5 μm 2.0 μm 0.5 μm

qty. 5-pk. 5-pk. 5-pk. 5-pk.

cat.# 25022 25023 25057 25990


LC COLUMNS

HPLC Guard Cartridges Trident HPLC Guard Cartridges Description Pinnacle DB Guard Cartridges Pinnacle DB C18 Guard Cartridge Pinnacle DB C8 Guard Cartridge Pinnacle DB Aqueous C18 Guard Cartridge Pinnacle DB Biphenyl Guard Cartridge Pinnacle DB PFP Propyl Guard Cartridge Pinnacle DB Cyano Guard Cartridge Pinnacle DB Silica Guard Cartridge Ultra Guard Cartridges Ultra C18 Guard Cartridge Ultra C8 Guard Cartridge Ultra C4 Guard Cartridge Ultra C1 Guard Cartridge Ultra Aromax Guard Cartridge Ultra Aqueous C18 Guard Cartridge Ultra Biphenyl Guard Cartridge Ultra IBD Guard Cartridge Ultra PFP Guard Cartridge Ultra Cyano Guard Cartridge Ultra Amino Guard Cartridge Ultra Silica Guard Cartridge Viva Guard Cartridges Viva C18 Guard Cartridge Viva C8 Guard Cartridge Viva C4 Guard Cartridge Viva Biphenyl Guard Cartridge Viva PFP Propyl Guard Cartridge Viva Silica Guard Cartridge

3-pk. (10 x 2.1 mm)

3-pk. (10 x 4.0 mm)

941450212 941350212 941850212 940950212 941950212 941650212 941050212

941450210 941350210 941850210 940950210 941950210 941650210 941050210

917450212 910350212 910250212 910150212 912750212 917850212 910950212 917550212 917650212 910650212 910750212 910050212

917450210 910350210 910250210 910150210 912750210 917850210 910950210 917550210 917650210 910650210 910750210 910050210

951450212 951350212 951250212 951650212 951950212 951050212

951450210 951350210 951250210 951650210 951950210 951050210

RESTEK

Guard Cartridge

USLC®

Ultra Selective Liquid Chromatography

Choose Columns Fast. Develop Methods Faster. See pages 137–139. www.restek.com/uslc

www.restek.com

171

LC Columns  

LC Columns 2013 catalog

LC Columns  

LC Columns 2013 catalog

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