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recommends periodical maintenance and calibration of the pipette and in most cases, the maintenance kit is also provided along with the pipette box. But unfortunately, 90% of the cases, the pipette remains in the same way without any maintenance. Today, most of the common clinical chemistry parameters are calibrated using a multi calibrator, which is usually presented in lyophil ised form. The calibrator needs to be diluted with specific volume of distilled water and in case the same is diluted using an old non-calibrated pipette, it can make serious error in the entire parameter calibration. The condition will be even worse when we use serial dilution in calibration. Since the fluid calibration is highly technical and requires dedicated costly instrument and special environment. It is always recommended to calibrate the pipette from an accredited agency. It is recommended to have two sets of calibrated pipettes with different calibration expiry. This will make sure that the laboratory is having calibrated pipettes in all time.

Temperature Monitor The reagent kits are highly sensitive and needs to be kept at 28 degrees to retain the stability and sensitivity as claimed in the

The reagent kits are highly sensitive and needs to be kept at 2-8 degrees to retain the stability and sensitivity as claimed in the manufacturer kit inserts. Certain specific reagents are highly temperature sensitive and a few hours in temperature below zero degrees can create reagent instability.

manufacturer kit inserts. Certain specific reagents are highly temperature sensitive and a few hours in temperature below zero degrees can create reagent instability. Monitoring of the cold room temperature is essential for assuring the reagent stability which is one of the major factor defining the accuracy of results. How do we confirm that the temperature in the cold room is accurate? Majority of the laboratories depend on the builtin temperature monitor of the cold room/Fridge or incubator. There may be a chance of error and it is advisable to use an electronic calibrated temperature monitor to read the temperature of the cold rooms, incubators etc in the laboratory. For laboratories having 24 hours operation, a room temperature monitor having maximum-minimum reading is ideal to monitor the environmental condition. It is recommended that a chart is pasted on the doors of the cold room or Fridge and the temperature is monitored at least twice in a day. For incubators and dry bath, makes sure that the temperature is checked each time a test is performed. Laboratory manager should check the chart at least on a weekly basis and make sure that there are no recurring issues happening. Laboratory should maintain a calibration record file for all the critical hardware systems and should make sure that the calibration is renewed properly to assure the quality of the tests performed.

TECHAGAPPE

APRIL-JUNE 2018

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Techagappe 15th edition (April - June 2018) E-book  

Agappe Diagnostics Limited is the first Indian IVD company publishing an International Diagnostic News Journal “Techagappe” for the public t...

Techagappe 15th edition (April - June 2018) E-book  

Agappe Diagnostics Limited is the first Indian IVD company publishing an International Diagnostic News Journal “Techagappe” for the public t...

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