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National Institute of Science and Technology of Viral Hemorrhagic Fevers (INCT-FHV) Management committee Pedro F. C. Vasconcelos (Coordenador) Márcio Roberto Teixeira Nunes (Vice-coordenador)

without necrotic evidences in endothelial cells, however demonstrated signals of activated endothelial cells (increased pseudopodia and increased trans-cellular channels).

Main researchers Elizabeth Salbé Travassos da Rosa, Ana Cecília Ribeiro Cruz, Daniele Barbosa Medeiros; Eliana Vireira Pinto of Silva, Lívia Caricio Martins, Manoel do Carmo Pereira Soares, Nelson Ribeiro Bailão, Heloísa Marceliano Nunes

Main publications Gomes-Gouvêa MS, Soares MCP, Bensabath G et al. (2009) Hepatitis B vírus and hepatitis delta vírus genotypes in outbreaks of fulminant hepatitis (Labrea Black fever) in the western Brazilian Amazon region. J Gen Virol. 90: 2638-2643. Mendes WS, Silva AAM, Neiva RF et al. (2010). Serologic survey of Hantavirus infection, Brazilian Amazon. Emerg Infect Dis 16(5): 889-891. DOI: 10.3201/eid1605.090766 Figueiredo MAA, Rodrigues LC, Barreto ML et al. (2010) Allergies and diabetes as risk factors for dengue hemorrhagic fever: results of a case control study. PLoS Negl Dis 4(6): e699. doi:10.1371/journal.pntd.0000699 Weidmann M, Faye OUS, Faye OUM et al. (2010) Improved LNA probe based assay for the detection of African and South-American Yellow Fever Virus strains. J Clin Virol. 48: 187-192. doi:10.1016/j.jcv.2010.04.013 Nunes MRT, Travassos da Rosa ES, Medeiros DBA et al. (2010) Circulation of hantaviruses in the influence área of the Cuiabá-Santarém (BR-163) Highway. Mem Inst Oswaldo Cruz. In press. Silva LK, Blanton R, Parrado AR et al. (2010) Dengue hemorrhagic fever is associated with polymorphisms in JAK1. Europ J Human Gen. In press.

Main research topics 1). Development of an immunochromatographic test for the rapid diagnosis of the Dengue virus using recombinant proteins of the Envelope (E) and monoclonal antibodies; 2). Development of an ELISA test for detection of NS1 antigen for Yellow Fever virus; 3). Development of an experimental model of dengue for study of infections of the central nervous system (encephalitis and encephalomyelitis) caused by dengue virus; 4). Development of genetic studies for association of polymorphism of genes KIR and their ligant groups HLA-C with Dengue; 5). Development of immunoenzymatic, RT-PCR and real time PCR tests for characterization of infections by Brazilian hantaviruses; 6). Development and standardization of biomolecular techniques for detection, quantification and genotyping of the viruses of the hepatitis B and D in cases of striking hepatitis; 7). Viral hemorrhagic fevers: characterization of the immune innate tissue response in situ and the role of the vascular phenomena in human and experimental infections in a monkey model. Summary of achievements and perspectives 1. Currently, the complete nucleotide sequences of 51 dengue virus (DENV) strains were obtained (19 DENV-1, 19 DENV-2 e 13 DENV-3). 2. During the first year, 13 YFV strains were completely sequenced, these represent the first genetic data (complete genome) for South American YFV isolates recovered from humans, monkeys and hematophagous insects, as well as from vaccine associated severe viscerotrophic syndrome. 3.It was developed a mice model to investigate the neurological symptomatology of dengue focusing on encephalitis caused by an escape mutant neurologic dengue-3 variant in the Laboratory of Neuroanatomy. 4. The present study aims investigate the association of KIR gene cluster polymorphism with Dengue clinical forms including dengue hemorrhagic fever (DHF). 5. Presently, 38 strains of Brazilian hantaviruses isolated from humans from different states (Mato Grosso-MT, Pará-PA, Amazonas-AM, and Maranhão-MA) had the N gene partially (450 nt) sequenced. 6. During the first year was emphasized the biomolecular standardization of the PCR methodology for detection of the viruses of the hepatitis B and Delta in the cases of fulminant hepatitis (acute liver failure) and implantation of the genotyping of those viruses for the characterization of the genomic sequences. 7. The ultra-structural analysis of VHF showed vascular structures

Contacts Rodovia BR-316, Km 07, 67030-000 Ananindeua, PA Tel: (91) 3214-2271 Email: pedrovasconcelos@iec.pa.gov.br http://inct.iec.pa.gov.br/index.htm

Nucleotide sequencing facilities

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livroinct2010  

Carlos Alberto Aragão de Carvalho Filho Presidente do CNPq 1

livroinct2010  

Carlos Alberto Aragão de Carvalho Filho Presidente do CNPq 1

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