Allergen uptake of DCs reaches a threshold
DC [100 µg/ml Phl p5]
[10 µg/ml Phl p5] 10.3±0.9
10 5
CD1a+ oLCs
4°C (Control)
10 5
10
4
10
4
10
3
10
3
10
2
10
2
8.1±2.1
0
0
0
10 2
10 3
10 4
10
4
10
3
10
2
0
10 2
10 3
10 5
10 4
10 5
45.2±3.5*
0
4
10
4
10
4
10
3
10
3
10
3
10
2
10
2
10
2
0
0
0
10 2
10 3
10 4
10 5
10 2
10 3
10 4
10 5
51.1±3.9*
10 5
10
37°C
13.7±2.3
10 5
0
10 5
17.0±0.1
10 5
[1000 µg/ml Phl p5]
0
0
10 2
10 3
10 4
10 5
0
10 2
10 3
10 4
10 5
FITC-Phl p5
allergen dose 16
Allam et al, J Allergy Clin Immunol 2010 29 September 2012