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Document details

Isolation, Sequence Identification and Tissue Expression of a Novel Tobacco (Nicotiana Tabacum) Gene

Published on Aug 11, 2014

Science
Shirley Wang
About

http://www.seipub.org/bab/paperInfo.aspx?ID=10984 The complete coding sequence of one tobacco (Nocotiana tabacum) gene-GDP-mannose 3',5'-epimerase (GME1), was amplified based on two tobacco ESTs. The complete coding sequence of tobacco GME1 gene was 1,131bp which encodes a protein of 376 amino acids. Sequence analysis revealed that the GME1 of tobacco shares high homology with the GME1 of lycopersicon esculentum (96%), peach (95%), castor bean (94%), malpighia glabra (94%), ribes nigrum (Accesession number: ACM90324, 93%), cucumber (92%), camellia sinensis (92%), soybean (92%). The expression profile was studied and the results indicated that tobacco GME1 gene was differentially expressed in detected tobacco tissues including leaf, stem, root and flower.

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