Page 1

Pedro H. Filsner

Luiz Felipe Caron

Celso Fávaro Jr.

Max Ingberman

Breno C. B. Beirão

TECHNICAL

Disinfecting bedding to reduce Salmonella challenge in broilers

BRENO C. B. BEIRÃO, MAX INGBERMAN, CELSO FÁVARO Jr., LUIZ FELIPE CARON and PEDRO H. FILSNER* report that a dry broad-spectrum disinfectant can effectively reduce Salmonella recontamination in poultry bedding, resulting in reduced cecal counts of Salmonella in broilers. Background While antibiotics such as polypeptides, fluoroquinolones and sulfonamides have been typically used to treat Salmonellosis, the rising need to reduce or eliminate the use of antibiotics in animal production systems means that disease prevention is gaining increasing priority. 10

The environment contaminated with Salmonella is the main source of infection in broiler poultry, thus environmental control is of great importance in management strategies of these agents. It is known that the intestinal microbiota of chickens is directly influenced by the microbiome found ASIAN POULTRY MAGAZINE – March 2018


Table 1: Identification of experimental groups used in the test.

Challenge

Treatment

Sample No.

Stalosan F + Salmo

S. Heidelberg

+

10

Stalosan F

S. Heidelberg

-

10

in the bedding. The assessment of cecum and bedding by the 16S rRNA sequencing technique — which identifies and quantifies all genera present in a sample demonstrates this relationship. Thus, bedding management is of great relevance for control of intestinal microbiota in birds. The effect of the bedding on the intestinal microbiota of birds seems to be more relevant in early life; at this stage, the correlation between the two microbial populations (environmental and animal) is more important. Therefore, the present study aimed to evaluate the effect of bedding treatment with the disinfectant Stalosan F on the contamination of the bedding itself and the cecum of broilers experimentally challenged with Salmonella.

Figure 1: Quantification of Salmonella in bedding.

6.00E+07 5.00E+07 4.00E+07

cfu/g

Treatment

3.00E+07 2.00E+07 1.00E+07 0.00E+00

Salmo+Stalosan

Salmo

Results present mean ± SD. The indicated P refers to the Mann-Whitney test.

Figure 2: Quantification of Salmonella in the cecum.

Material and methods

ASIAN POULTRY MAGAZINE – March 2018

1.20E+09 1.00E+09 8.00E+08

cfu/g

Test conducted on isolators. Installation configuration; stainless steel isolators with forced ventilation, filtered air, external manipulation to the isolator, triple-paper bedding, lamp heater, nipple-type drinker and trough feeder. Environment decontaminated with formalin fumigation. The test comprises the microbiological evaluation of ceca of broiler chickens and bedding. The groups are shown in Table 1. Before starting the test, isolators were evaluated to rule out the presence of contaminating Salmonella. The birds were maintained with feed appropriate for their age and water ad libitum, from the first day of life to the seventh day of life. Stalosan F was applied on the bedding at day one at a 50 g per square meter concentration. On day one, broilers in the Salmo and Stalosan F + Salmo groups received an oral challenge with

6.00E+08 4.00E+08 2.00E+08 0.00E+00 Salmo+Stalosan

Salmo

Results present mean ± SD. The indicated P refers to the Mann-Whitney test.

108 cfu/bird of tetracycline-resistant S. Heidelberg isolated from field, individually applied. At seven days of life, three bedding samples from each group were collected in sterile packaging. Twentyfive grams of each sample (or the nearest maximum weight) were

diluted proportionally in buffered peptone water with tetracycline and microbiologically analysed in bright green agar with tetracycline. The Salmonellae in the cecum were also quantified on day seven through selective culture of the samples on bright green agar with 11


tetracycline after dilution in buffered peptone water with tetracycline. Since the bedding consisted of paper, and as the forced air flow in the isolators inevitably keeps the bedding relatively dry, the moisture content has not been evaluated.

TECHNICAL

Results In the analysis of bedding in the isolator conditions, the group that received Stalosan F and challenge had a statistically lower count than the challenged-only group (P = 0.05) (Figure 1). The quantification of Salmonella in the ceca of animals presented a similar result to that seen in bedding in the same condition; thus, the group treated with Stalosan F had a reduction in the Salmonella count in the cecum when compared to the group receiving only Salmonella (Figure 2).

Discussion The present study aimed to evaluate the effect of Stalosan F on the bedding contamination of chickens challenged with Salmonella enterica, as well as on the cecal

12

count of these agents. In order to understand the effect of the product under conditions where there is no environmental persistence of Salmonella from untreated groups, the experiment was performed in isolators. Under these conditions, bedding treatment with Stalosan F reduced the Salmonella count both in the bedding and cecum of challenged birds. In isolators, paper must be used as bedding, to prevent blockage of filter elements that would occur with the use of wood shaving bedding. In the literature, in the comparison between wood shaving and rubber bedding, there was little difference found in horizontal transmission of Campylobacter spp. Paper and wood shaving bedding do not show great differences regarding microbiota, indicating that the results of this study in isolators are significant in the field. Therefore, bedding treatment with Stalosan F proved to be effective in reducing environmental contamination generated by experimentally-infected broilers. The reduction of the recontamination of

these same animals must justify the lowest Salmonella count in the ceca of birds in the Stalosan F group.

Conclusion Stalosan F was effective in reducing bedding contamination by broilers experimentally infected with S. Heidelberg. Cecal counts of Salmonella in treated animals were also reduced. AP

*Breno C. B. Beirão (study coordinator), Max Ingberman (R&D Director), Celso Fávaro Jr. (Quality Director), are with Imunova Análises Biológicas. Luiz Felipe Caron (professor of Microbiology and Vaccinology), is with the Universidade Federal do Paraná. Pedro H. Filsner (R&D Analyst, study monitor), is with Sanphar. References are available on request (office@sanphar.net).

ASIAN POULTRY MAGAZINE – March 2018

Stalosan f salmonella asian poultry pydbrs 1302  

Disinfecting bedding to reduce Salmonella challenge in broilers Trial by Sanphar

Stalosan f salmonella asian poultry pydbrs 1302  

Disinfecting bedding to reduce Salmonella challenge in broilers Trial by Sanphar