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KPC + MBL Confirm ID kit For identification of Metallo-ß-lactamases and KPC enzymes (Class A)

K.pneumoniae PHA3 CL5761 KPC positive

3 3 3 3 3

Simple to test Easy to interpret Long shelflife After opening, room temperature storage (≤ 25°C) High sensitivity and specificity

Carbapenemases should be suspected if: Ertapenem zone ≤ 22 mm (Enterobacteriaceae) or meropenem zone < 25 mm (Enterobacteriaceae/P. aeruginosa).

MBL suspected if: • Resistance to ceftazidime • No synergy Cephalosporin/Clavulanate • Reduced susceptibility to carbapenems

KPC show: • Negative metallo-β-lactamase test • Synergy between Boronic acid and Carbapenems • No synergy between Cloxacillin and Carbapenems


KPC + MBL Confirm ID kit Combined disk test Apply one tablet of: Meropenem (MRP10) Meropenem+DPA (MR+DP) Meropenem+Boronic (MR+BO) Meropenem+Cloxacillin (MR+CX) on an inoculated Mueller Hinton agar plate (McFarland 0.5).

Interpretation (combined test) Meropenem + Boronic MR+BO

Meropenem + DPA MR+DP

Meropenem + Cloxacillin MR+CX

AmpC + porin loss

Meropenem 10 µg MRP10

≥ 5mm AND

< 5mm

≥ 5mm

KPC

Meropenem 10 µg MRP10

≥ 5mm

< 5mm

< 5mm

MβL

Meropenem 10 µg MRP10

< 5mm

≥ 5mm

< 5mm

Neither AmpC, KPC nor MβL: All zones within 2 mm of each other.

For detailed information about the test, please also look in: • Detection of resistance mechamisms using Neo-Sensitabs™ and Diatabs™, document 1.5.0 • Instructions for use

One kit containing (50 tests): 1 cartridge of Meropenem 1 cartridge of Meropenem+DPA 1 cartridge of Meropenem+Boronic 1 cartridge of Meropenem+Cloxacillin

Edition: 3A

Taastrupgaardsvej 30, DK-2630 Taastrup

www.rosco.dk

/kpc_print  

http://www.rosco.dk/gfx/kpc_print.pdf