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Inthistypeofassay,oneofthereactioncomponentsisnonspecificallyadsorbedorcovalentlyboundtothesurfaceofasolidphase,suchasamicrotiterwell,a magneticparticle,oraplasticbeadABSTRACTELISA(Enzyme-linkedimmunosorbentassay)isatechniqueusedtoassessthequantificationofpeptide, protein,antibodyandhormonelevels,basedontheprincipleofTheantibodiesboundthecontrolantigenthathadELISA(enzyme-linkedimmunosorbentassay)is aplate-basedassaytechniquedesignedfordetectingandquantifyingpeptides,proteins,antibodiesandhormonesOriginallydescribedbyEngvallandPerlmann(), themethodenablesanalysisofproteinsamplesimmobilizedinmicroplatewellsusingspecificantibodiesWhatisanELISA?AnELISA,likeothertypesof immunoassays,reliesonantibodiestodetectatargetantigenusinghighlyspecificantibody-antigeninteractionsIn,ELISAwasintroducedbyPeterPerlmannand EvaEngvallatStockholmUniversityinSwedenInanELISA,anantigenmustbeimmobilizedtoaELISAstandsforenzyme-linkedimmunosorbentassay,also oftenreferredtoasenzymeimmunoassay(EIA).Enzyme-linkedimmunosorbentassay(ELISA)isanimmunologicalassaywidelyusedinbasicscienceresearch, clinicalapplicationstudies,anddiagnostics.TheELISAThevolumeperwellshouldbethesameasthecaptureantibodyusedinstepRemovetheblockingbuffer andaddthesamplesandstandardsELISAisanantigenantibodyreactionItisacommonlaboratorytechniquewhichisusuallyusedtomeasuretheconcentration ofantibodiesorantigensinbloodInanELISAassay,theantigenmustbeimmobilizedtoasolidsurfaceTheenzymelinkedimmunosorbentassay(ELISA)isa powerfulmethodfordetectingandquantifyingaspecificproteininacomplexmixtureELISA(whichstandsforenzyme-linkedimmunosorbentassay)isa techniquetodetectthepresenceofantigensinbiologicalsamplesAnELISA,likeothertypesofimmunoassays,reliesonantibodiestodetectatargetantigenusing highlyspecificantibody-antigeninteractionsELISA(enzyme-linkedimmunosorbentassay)isaplate-basedassaytechniquedesignedfordetectingandquantifying peptides,proteins,antibodiesandBasicPrinciplesofELISAReactionSchemesThischapterintroducesthebasictestformatsfortheperformanceofsolid-phase heterogeneousELISAAnELISA,likeothertypesofimmunoassays,reliesonantibodiesMÉTHODEIMMUNO-ENZYMATIQUEELISAI)ObjectifsLe termeELISAaétéemployépourlapremièrefoisparENGVALL&PERLMANN()[1]BasicELISAprinciplesEnzyme-linkedimmunosorbentassay(ELISA) isaheterogeneousEIAtechniqueusedinclinicalanalysesThisformofELISAELISA-Principle,TypesandApplicationsELISAisanantigenantibodyreaction ELISAisaplatebasedassaytechniquewhichAnexampleofacompetitionELISAtotestforantigenbasedonthedirectdetectionmethodisshownin FigureRemoveliquidandwashplateRemoveliquidandwashplateRemoveliquidandwashplateRemoveliquidandwashplateInthisexampletheantigen concentrationinthesamplewaslowIntroductionBasicELISAprinciplesCovertheplateandincubateforELISAstandsforenzyme-linkedimmunosorbent assay,alsooftenreferredtoasenzymeimmunoassay(EIA).In,ELISAwasintroducedbyPeterPerlmannandEvaEngvallatStockholmUniversityinAbstract.In anELISAELISA-Principle,TypesandApplicationsCetteméthodeestAnELISA,likeothertypesofimmunoassays,reliesonantibodiestodetectatarget antigenusinghighlyspecificantibody-antigeninteractions