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Presentation Title: Expression Analysis of Genes Encoding Members of a Ciliary Central Pair Protein Complex Discipline: Reproductive and Cellular Biology School: Augustana College Presentation Type: Poster Presentation Abstract: Expression Analysis of Genes Encoding Members of a Ciliary Central Pair Protein Complex Taylor Maier1,2, Casey McKenzie3, Andrew Cardillo3and Lance Lee3 1Department of Biology, Augustana College, Sioux Falls, SD 57197; 2Sanford Program for Undergraduate Research (SPUR), Sanford Research USD, Sioux Falls, SD 57104; 3Sanford Children’s Health Research Center, Sanford Research USD, Sioux Falls, SD 57104

Primary Ciliary Dyskinesia (PCD) is a rare autosomal recessive disease that is characterized by the dysfunction of motile cilia motility. It was discovered that three proteins that associate with the central pair complex were regulating cilia beating in the single-cell organism Chlamydomonas. The homologous genes to these were previously uncharacterized in mammals. Thus, the research featured in this poster was the first characterization of these genes, in their murine homologs. The goal of this experiment was to analyze expression levels of these genes in mice, one group of which were mutants who had a PCD phenotype and the other a wild-type control group. To examine expression levels qPCR, Taqman approach, was recorded on seven different tissues from each mouse. The tissues were selected to give a sampling of both motile ciliated and non-ciliated tissues. Preliminary data taken has suggested that the murine homologs do in fact play an important role in motile cilia beating. This could have major implications because it is likely that if these genes play a role in ciliated tissue of mice then they will also be important in mammalian motile ciliated tissues as a whole.

Presenter: Taylor Maier Tribe: Rosebud Sioux Tribe Primary Email: tmmaier09@ole.augie.edu Biography My name is Taylor M. Maier and I am a junior at Augustana College in Sioux Falls, SD. I am a biology (pre-dentistry) major and hope to attend dental school after graduating from Augustana in 2013. I was born and attened high school in Pierre, SD. I am a member of the Rosebud Sioux Tribe here in South Dakota and hope to practice dentistry on my reservation someday.

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2011 National Conference Student Research Abstracts  
2011 National Conference Student Research Abstracts  

A comprehensive list of the student research topics that will be presented at the AISES 2011 National Conference

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