Presentation Title: Binding of CD47 and VEGFR2 Associated with Angiogenesis Discipline: Biology School: ¹Department of Biology, San Juan College, Farmington NM, ²Department of Chemistry and Biochemistry, Northern Arizona University, Flagstaff, AZ Presentation Type: Poster Presentation Abstract:
Binding of CD47 and VEGFR2 Associated with Angiogenesis 1 2 Nathaniel Jim , Matthew J. Gage , Ph.D. ¹Department of Biology, San Juan College, Farmington NM, ²Department of Chemistry and Biochemistry, Northern Arizona University, Flagstaff, AZ
Vascular endothelial growth factor A (VEGF-A) is key for developmental angiogenesis and a major drug target for inhibiting angiogenesis in cancer. Thrombospondin 1 (TSP1) was the first identified endogenous inhibitor of angiogenesis, but the mechanism underlying this activity is still unclear. TSP1 was recently discovered to inhibit NO signaling through a mechanism involving binding to receptor CD47, which constitutively binds VEGFR, the VEGF-A receptor, in an interaction that is disrupted upon binding of TSP1, rendering VEGFR inactive. Thus, TSP1 represents an outstanding new target for intervention in angiogenesis-dependent cancer proliferation, as well as for cardiovascular disease and wound healing abnormalities, but little is known about how it functions. The focus of this project is to identify the amino acids critical to this interaction using a FRET analysis system designed by the David Robert’s group. Mutations will be generated in the CD47 gene and their ability to disrupt the FRET signal will be used to determine critical amino acids involved in the interaction between CD47 and VEGFR2. It is hypothesized that this interaction is mediated by the extracellular Ig domain in CD47, which is the focus of this study. Preliminary data of 3T3 cells transfected with CD47-eGFP exhibits increased florescence at 520 nm. Further research is needed in discovering the functions and processes of CD47.
Presenter: Nathaniel Jim Tribe: Navajo Primary Email: firstname.lastname@example.org Biography Greetings I am Nathaniel Jim. I am majoring in biology at San Juan College a two year institution in Farmington, NM. I intend on transferring to a four-year university where I will earn a bachelor’s of science in Biology, then continue to graduate school to earn my medical doctor’s degree. My career plans are to work as a biochemist researching cancers and viruses. Furthermore I have been obligated to experience the Native American Cancer Prevention (NACP) internship researching the processes by which viruses and diseases are organized and studied. I am working toward a protein based cancer research occupation.
Published on Oct 20, 2011
Published on Oct 20, 2011
A comprehensive list of the student research topics that will be presented at the AISES 2011 National Conference