Page 30

Presentation Title: Identification of Heparan Sulfate 3-O-Sulfotransferase Using Proteomics Approach Discipline: Bioengineering School: University of Hawaii at Manoa. Research done at the Renssalear Polytechnic Institute. Presentation Type: Poster Presentation Abstract: Identification of Heparan Sulfate 3-O-sulfotransferase 1 Using Proteomics Approach Raquel Auwae Mentor: Dr. Lingyun Li Rensselaer Polytechnic Institute

Peptide Mass Fingerprinting (PMF) is an analytical technique used to understand the identity of a protein by breaking the protein down to the peptide level. These masses of peptides can be compared to a database containing known protein sequences. The database statistically analyzes the unknown protein to find the best match with a theoretical protein sequence. Heparan sulfate 3-O-sulfotransferase isoform1 (3-OST1) was the unknown protein used in this experiment to be analyzed by a protein sequence database. Results from the database yielded a highly confident match with the 3-OST1 found in a cloned mouse.

Presenter: Raquel Auwae Primary Email: rauwae2@hawaii.edu Biography Raquel Auwae is a sophomore studying Engineering at the University of Hawai`i at Manoa. She is apart of several Native Hawaiian organizations such as the Native Hawaiian Science & Engineering Mentorship Program and the new American Indian Science & Engineering Society chapter in Hawai`i. Her interests are in sustainability and biotechnology. She hopes to further her studies so that she may help save the environment.

29

2011 National Conference Student Research Abstracts  

A comprehensive list of the student research topics that will be presented at the AISES 2011 National Conference

2011 National Conference Student Research Abstracts  

A comprehensive list of the student research topics that will be presented at the AISES 2011 National Conference

Advertisement