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Presentation Title: Investigation of Uranium Accumulation in Mice Organs Discipline: Chemistry School: Northern Arizona University Student Level: Masters Presentation Type: Oral Presentation Abstract: Investigation of Uranium Accumulation in Mice Organs Venessa Jim, Jani C. Ingram, PhD Department of Chemistry & Biochemistry, Northern Arizona University Margaret M. Briehl, PhD Arizona Cancer Center, University of Arizona

Uranium is a toxic heavy metal and is prevalent in certain areas of the Southwest, more specifically the Navajo reservation. In these communities the risk of exposure is high due to past uranium mining activity; in addition, most sites have not been properly remediated. The aim of this project is to better understand where uranium deposits in certain organs of mice, specifically observing the liver and kidney. Laser ablation-inductively coupled plasma mass spectrometry (LA-ICP-MS: UP -213 Newman Research Laser Ablation System and VG Axiom MC) was used to determine levels of uranium-238 in specific locations of the liver and kidney. The experimental model simulates Navajo communities who are exposed daily to uranium in their drinking water and was done in collaboration with the University of Arizona. The samples were obtained from two different mice groups; one group was exposed for six months to specific concentrations of uranium in their drinking water, whereas the other was the control group. The organ samples were mounted in optimum cutting temperature compound (OCT) and then frozen sectioned via cryostat. Samples with thicknesses ranging from 5-10 Âľm were then analyzed by LA-ICPMS. In kidney samples, the concentration of uranium was compared between the cortical and medullar regions; and for the liver, the center and the edge of the samples were compared. Initial results show that different organs as well as the areas within the organs have different concentrations of uranium.

Presenter: Venessa Jim Tribe: Navajo Primary Email: vqj@nau.edu Biography Venessa Jim is currently a graduate student at Northern Arizona University working on a master's degree in chemistry & biochemistry. Venessa graduated from NAU in summer 2009. Venessa is a member of the Navajo Nation, and is orginally from Fruitland, NM. Venessa is in the process of applying to PhD schools in biochemistry and plans to begin her studies in fall 2012.

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2011 National Conference Student Research Abstracts  
2011 National Conference Student Research Abstracts  

A comprehensive list of the student research topics that will be presented at the AISES 2011 National Conference

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