2013 PRC Annual Report

The PRC Annual Report 2012

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Vision Excellence in research and learning through partnerships with the entire value chain to advance the development of value-added poultry products and production practices

Goals To conduct excellent research that leads to the development of innovative and sustainable production systems To serve as a leading source of scientific knowledge that supports the production of safe, high quality poultry products that meet changing consumer needs To foster an environment of learning that incorporates input from industry, as well as teaching, technology transfer and knowledge transfer activities To connect with industry in a continuous manner, both in the receiving of input and the transferring of knowledge

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The PRC Annual Report 2013

Table of Contents Chair’s Report ............................................................................................................................................... 5 Academic Leader’s Report ............................................................................................................................ 6 Technology Transfer Highlights .................................................................................................................... 7 Highlights: Education, Training & Retention of Highly Qualified People ...................................................... 8 Business Development Highlights ................................................................................................................. 9 Stakeholder Reports ................................................................................................................................... 10 Alberta Agriculture and Rural Development .......................................................................................... 10 Alberta Chicken Producers...................................................................................................................... 10 Alberta Turkey Producers ....................................................................................................................... 12 Burnbrae Farms....................................................................................................................................... 12 Egg Farmers of Alberta............................................................................................................................ 13 Egg Farmers of Canada ........................................................................................................................... 14 Maple Leaf .............................................................................................................................................. 15 University of Alberta ............................................................................................................................... 15 Awards ........................................................................................................................................................ 16 Faculty Awards ........................................................................................................................................ 16 Graduate Student Awards....................................................................................................................... 16 Graduations................................................................................................................................................. 17 Research Highlights ..................................................................................................................................... 18 Eduardo Beltranena ................................................................................................................................ 18 Mirko Betti .............................................................................................................................................. 18 Valerie Carney ......................................................................................................................................... 19 Doug Korver ............................................................................................................................................ 19 Lynn McMullen ....................................................................................................................................... 19 Aman Ullah.............................................................................................................................................. 20 Wendy Wismer ....................................................................................................................................... 20 Jianping Wu ............................................................................................................................................. 20 Martin Zuidhof ........................................................................................................................................ 22 Organizational Structure ............................................................................................................................. 23 Board ....................................................................................................................................................... 23 The PRC Annual Report 2013

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PRC Operations Personnel ...................................................................................................................... 24 Researchers ............................................................................................................................................. 25 Graduate Students .................................................................................................................................. 26 Visiting Students and Scholars ................................................................................................................ 27 Technical Support ................................................................................................................................... 27 Post-Doctoral Fellows ............................................................................................................................. 28 Research Associates ................................................................................................................................ 28 Research Projects ($3,228,414) .................................................................................................................. 29 PRC Financials 2012-2013 ........................................................................................................................... 35 Income 2012-2013 .................................................................................................................................. 35 Expenses 2012-2013 ............................................................................................................................... 37 Revenue Budget 2013-2014.................................................................................................................... 38 Expense Budget 2013-2014 .................................................................................................................... 39 Revenue/Expense Summary 2013-14 ..................................................................................................... 39 Poultry Unit Financial Report 2012-2013................................................................................................ 40 Poultry Unit Budget 2013-2014 .............................................................................................................. 41 Facility Usage .............................................................................................................................................. 42 Evidence of Productivity ............................................................................................................................. 43 Articles published in refereed journals (n=26) ................................................................................... 43 Proceedings ......................................................................................................................................... 44 Presentations ...................................................................................................................................... 45 Research Reports ................................................................................................................................ 49 Trade articles....................................................................................................................................... 51 Patent Applications: ............................................................................................................................ 51 Acronyms and Abbreviations Used ............................................................................................................. 52

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The PRC Annual Report 2013

Chair’s Report It has been a year and a half since I accepted the position of Chair of the Advisory Board at the Poultry Research Centre. During that time there have been many changes at the Centre. There have been some remarkable achievements, new members added to our team and we have successfully implemented a new agreement.

There is now a very solid 5 year contract set in place. A tremendous amount of work went into the finalizing of this contract. John Bell and colleagues are to be congratulated. Now we can get back to the business of research and education.

The student poultry club has really taken flight with approximately 40 members many of which are not studying poultry as a career. This is a hands-on approach to teach young people about agriculture and may even land us a few new recruits! Club members have enjoyed many field trips and have been instrumental by participating in the minion labour for experimental projects and have even worked for local chicken farms! These students have been instrumental with helping Agnes Kulinski with her Heritage Poultry project. If successful, this project will help the Heritage breeds of poultry at the Centre pay their way and give direction to other groups endeavouring to promote heritage breeds.

The Centre has enjoyed the benefits of new staff and new collaborations this year. We were very fortunate to add Agnes Kulinski to our team. She is the new business development officer and has hit the ground running! The Centre is indebted to AI Bio for providing funding for Agnes’ position.

She has been a true asset to the Centre. The Centre is also enjoying a successful partnering with the Poultry Health Services and Dr. Tom Inglis. We look forward to future collaborations.

Congratulations to all the graduating graduate students at the university. The Centre continues to have a large number of graduate students. Eleven students have moved on this year- some to a career in poultry, some to further studies.

2013 marks the beginning of the next Growing Forward program administered by the federal government. We will look forward to this funding which will help finance poultry research and agriculture research in general. With global issues including imminent population growth, climate change and the ongoing issues with animal activists, agriculture research should be a very important component of the federal government’s strategy. Here at the University of Alberta, the Poultry Research Centre has the resources to help research these issues and find solutions. That is what we do very well.

Dr. Helen Anne Hudson The PRC Annual Report 2013

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Academic Leader’s Report Together we have built the PRC, which has become a model of industry, government and university collaboration. We should take a moment to enjoy that great accomplishment! As stakeholders in the PRC, we all strive to attain common goals relating to research, technology transfer, and training of highly qualified personnel. The research infrastructure is held at the University of Alberta, including the funding that facilitates poultry research, technology adoption efforts, and learning. Much of the leadership and communication also originates from the University and as a result, it is easy to assume that the University should be driving efforts at the PRC. That is usually the case when it comes to research and education. Through discussions over the past year, however, it has become clear that the way to maximize benefits to all PRC stakeholders is for each stakeholder to take responsibility for their role within the PRC. When it comes to taking advantage of the work that’s being done in your backyard, I want to encourage you to ‘stay hungry’!

Many who understand my love for music and teaching will not be surprised that I have been known to say, “There’s a song for everything”. I want to point you to the classic Bill Withers tune, Lean on Me: “For no one can fill those of your needs that you won't let show”. At the PRC we are eager and willing to help solve practical problems. We work hard to push out many of our research results, and over the last year we have had a spike in the number of industry meetings we attended. Still, by far the most gratifying and high impact conversations tend to be with people who call or email to share and find solutions to practical problems in the field. We get calls from all over the world because our expertise at the PRC is internationally recognized.

Make sure you as a stakeholder are first to benefit from your investment by letting us in on the challenges and opportunities out there.

This year we’ve asked for your input to the PRC annual report. We hope that this reflection triggers reflection, focus, and deeper engagement with the PRC. The 2012-13 year was a difficult year of transition, but we have also enjoyed some really great successes. We have been working hard with Agnes Kulinski, our new Business Development Officer, toward sharing PRC innovations. With her great leadership, we made remarkable progress this year toward sustainably preserving the heritage chickens at the PRC. We have watched some amazing young leaders develop in the undergraduate Poultry Club. As academic leader of the Poultry Research Centre, I’m very proud and excited to encourage you to read about our achievements and to think about how our knowledge can also contribute to your success. Thanks for your support!

Dr. Martin Zuidhof Page | 6

The PRC Annual Report 2013

Technology Transfer Highlights Connecting with the industry Research at the PRC spans the continuum from production to consumer and from basic to applied. In keeping with the goals of the PRC, researchers and students made a concerted effort in 2012 to connect with the industry. This took many forms from technical literature, workshops and presentations to commercial level research. The PRC appreciates the opportunity to work with producers, processors, hatcheries and private companies to transfer knowledge. Input, feedback and interaction with our partners and collaborators has helped us to be targeted, relevant and applicable. We appreciate the opportunity to work with you and look forward to more in the coming year.

Setting the stage for Success: A workshop for Canadian Hatchery Professionals Alberta Agriculture and Rural Development in cooperation with the PRC and Maple Leaf Foods organized a workshop for hatchery professionals. Following a national survey of hatchery personnel, poultry service personnel and researchers, the workshop was designed to meet the specific educational needs of the industry. Hatchery professionals, hatching egg producers, allied industry staff and students from across Canada learned from experts and from each other the latest techniques for producing high quality chicks and poults. Ninety-three percent of participants rated the content as good or excellent and 100% of participants said they would attend again.

Setting the stage for Success: Managing Breeders in the 21st Century Alberta Agriculture and Rural Development, the PRC and Aviagen worked together to deliver the fourth breeder management workshop. More than 70 poultry industry professionals representing every region of Canada were registered for the event – over twice the number of participants originally anticipated.

The workshop focused on sharing knowledge, collaboration, and best practices in broiler breeder management. The intense 3 day program featured seventeen presentations from academia, poultry production specialists, and allied industry on a variety of critical areas for successful production.

Attendees included hatching egg producers, hatchery and feed company representatives, as well as other poultry professionals. 94% or attendees said that they would implement new practices on their farm as a result of attending the workshop and 81% of them specified what they planned to change.

Obrigada Brazil!

The PRC was well represented at the 2012 World’s Poultry Congress held in Salvador Brazil. Six researchers and four graduate students shared their research through posters and oral presentations with a global contingent of poultry experts. Travel for two of the graduate students, Thania Moraes and Kim Tom, was covered by travel scholarships awarded by the World’s Poultry Science Association and the Canadian branch of WPSA. International meetings enable our researchers to interact with scientist from the around the world and to transfer global scientific knowledge back to the local industry.

Dr. Valerie Carney The PRC Annual Report 2013

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Highlights: Education, Training & Retention of Highly Qualified People Leadership in teaching PRC members play a major role in the undergraduate and graduate teaching programs in the Faculty of Agricultural, Life and Environmental Sciences (ALES). Over 200 undergraduate students at the 200 (introductory) level are taught by PRC members. Although these courses are often introductions to larger disciplines such as Animal Science and Food Science, a substantial portion of these courses is directly relevant to poultry, and often encourages students to take advanced poultry courses. In 2012, there were 2 undergraduate classes entirely focused on poultry, 8 courses with 5 to 90% poultry relevant material, and three poultry-focused independent study or Capstone projects involving 9 students. At the graduate level, PRC team members teach 4 courses that have 35 to 100% poultry relevance.

Training of highly qualified personnel (HQP) In addition to the technicians, Research Associates, Post-doctoral Fellows, Undergraduate Research assistants and Visiting Scientists (see elsewhere in this report), the PRC team continues to recruit and train excellent graduate students. In 2012, over 17 Ph. D. and 10 M. Sc. students were trained at the PRC. Of our recent graduates (5 Ph. D. students and 6 M. Sc. students), four have found employment in the poultry sector in Canada and internationally, and three have gone on to further studies in Canada. Our students are very competitive for scholarships and awards locally and internationally.

Recruitment An exciting development over the past year has been the start of an undergraduate Poultry Club. The club has around 40 enthusiastic members, mainly from the Faculty of ALES. Members have been on tours of commercial farms, processing plants and have caught chickens for Alberta producers. Members have received poultry handling training, and have become an important part of the data collection days during many research projects. Planned future activities include involvement with the PRC’s Rare Breeds program and more tours of commercial poultry operations and processing plants.

Integration with industry The PRC was well-represented at the 2013 Alberta poultry industry Regional Meetings in Red Deer. Nine PRC members, 4 technical staff, 8 graduate students and 8 undergraduate students attended, including a large number that were able to stay for the banquet for relationshipbuilding with the industry. Research posters were displayed and producer meeting presentations were given by the PRC team, and a group of the undergraduates handed out information on poultry barn ventilation from their Capstone course project. The PRC and the Poultry Club are working to further industry relationships through poultry industry internships.

Dr. Doug Korver Page | 8

The PRC Annual Report 2013

Business Development Highlights The PRC is an international leader in key areas of poultry research resulting in the development of innovative technologies, products and services. The Business Development position was created to capitalize on those technologies developed at the PRC and generate new revenue streams to build the sustainability and self-sufficiency of the PRC. Over the past year, two commercialization opportunities were identified – commercialization of heritage chickens and eggs, and specialized peptides from poultry by-products and we feel we have already made good progress.

The Heritage Chicken Project: For the last 20 years, the PRC has maintained small populations of 5 heritage chicken breeds which include Light Sussex, Barred Plymouth Rock, White Leghorn, New Hampshire, and Brown Leghorn.

After detailed market analysis and feasibility studies, the PRC has decided to develop a sustainable business plan for heritage chickens. Niche market development can permit and even encourage the use of non-standard breeds allow them to be self-sustaining. The PRC has launched a pilot program — “adopt a heritage chicken”, where the PRC raises the sponsor’s chicken, which provides a dozen heritage eggs to “the owner” every 2 weeks. The adoption fee is $75 for five months. The PRC has received overwhelming response from the public and media wanting to support the genetic preservation and our heritage breeds. Over 200 people joined the program in March with approximately 500 on the waiting list for November when the program will expand. We have raised over $15,000. Our heritage chicken supporters have been very happy with the program and eggs.

We feel this program is an excellent opportunity to connect with the public, educate and promote the PRC’s research and technologies.

Specialized peptides: I also have been involved in the commercialization of Dr. Betti’s specialized peptides from poultry by-products. Dr. Betti has developed a method of enzymatic glycosylation of protein isolated from poultry, beef and swine by-product. This new method of glycosylation increases protein/peptide functionality including solubility, gelling properties, antioxidant and antimicrobial activity. Flavor enhancement potential of this product as a salt substitute is currently under investigation. The research” has resulted in three patent applications filed in September 2012. The technology has attracted significant attention from the industry and we have hosted two visits from international companies, Tessenderlo Group and NeoCell, regarding collaboration.

While we have achieved much, there is still much more to be done to ensure sustainability and self-sufficiency of the PRC. While the effects of the budget cuts will most likely continue in 2013/14, opportunities may arise and we are ready and motivated to seize them.

Agnes Kulinski The PRC Annual Report 2013

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Stakeholder Reports Alberta Agriculture and Rural Development Alberta Agriculture and Rural Development’s (ARD) research activities are directed by the ARD’s 3 year business plan and 10 year strategic planning process. Recently the Livestock Research Branch was part of a restructuring of the Ministry to better align work units and size of the Divisions. The Livestock Research Branch was part of the former Research and Innovation Division, and aligned the Branch with the Crop research, Bioindustrial business development activities and Crop Research Centres in Edmonton, Lacombe and Brooks. The Branch is now aligned with the newly formed Livestock Research and Extension Division which also has the following Branches: Livestock Farm and Business; Traceability; and Alberta Ag-Info Centre.

The partnership between the Livestock Research Branch and the Poultry Research Centre is indispensable in fulfilling the core strategies to accelerate the adoption and commercialization of scientific knowledge and research outcomes and to cultivate collaborative research partnerships to identify and solve major industry challenges. In taking the lead on technology transfer for the centre, the Livestock Research Branch has developed strong industry connections through its delivery of relevant and applicable research solutions. The Poultry Research Centre’s excellence in research has been the foundation for this connection and has enabled ARD to advance into adaptive research to support the adoption of innovation in the industry.

Alberta Chicken Producers Alberta Chicken Producers Shared Industry Vision: To continue to grow, be profitable, and satisfy consumers by providing safe, high quality chicken products Alberta Chicken Producers Mission: To serve our producers by providing an environment for profitable chicken production and encouraging a competitive, consumer-focused chicken industry.

Alberta Chicken Producers 2012-13 Strategic Priorities:   

Alberta’s Allocation: Growing the Domestic Chicken Market in Alberta Strong Supply Chain Relationships: Supporting the Industry in Meeting Customer Volume and Product Specification Requirements Strong Industry Partnerships: Creating Opportunities For Industry Synergies

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The PRC Annual Report 2013

 

Antimicrobial Use (AMU): Exploring Reduction Strategies for Antibiotic Use and Educating Producers and the Public About the Issue Public Relations: Enhancing Awareness and Knowledge of Alberta’s Chicken Industry

In addition to these areas of focus, we are proud of the accomplishments of Alberta’s chicken industry this past year, which includes:    

100% Certification of Alberta’s 235 registered chicken producers under the mandatory Animal Care Program; 3rd Party Recognition from CFIA for the mandatory On-Farm Food Safety Assurance Program; Implementation of a Quality Expectations Document for broiler producers; and Implementation of a Direct Marketing Lease Program.

Looking ahead, Antimicrobial Use (AMU) is a critical issue that the chicken industry is assuming a leadership role in addressing. Alberta is supportive of the Chicken Farmers of Canada’s Five Point Strategy, which includes research into current usage and alternatives to antibiotics, controlling antibiotic use, and stakeholder education. Alberta Chicken Producers will also be undertaking further actions at a provincial level to better understand and quantify usage and educate our producers and industry partners with Alberta-based data to help guide our actions going forward.

Alberta Chicken Producers is a major partner in the Poultry Research Centre (PRC); and, has been engaged in this partnership from the PRC’s establishment in 1986. Our industry has cultivated a close working relationship and promotes open communication with the PRC to ensure its research and development themes are aligned with the priorities of Alberta’s poultry industry. This level of integration between researchers, industry and producers is a unique and innovative model that continues to meet the dynamic needs of our industry.

The current projects initiated by the researchers of the PRC specifically address our industry’s priorities of food safety, nutrition, and the development of value-added products. This year, Alberta Chicken Producers is funding the following projects: 1. Reducing Salmonella and Clostridium in Poultry 2. Incidence, Pathogenesis and Control Measures of Enterococcus Infections in Chicken Embryos and Neonatal Broiler Chickens in Alberta 3. Functionalized Peptides for Skin Care Produced from Bovine and Poultry Collagen Biomass

The PRC Annual Report 2013

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4. Immunoprotection in Alberta Broiler Chickens: Comparing the Efficacy of Live Inactivated Fowl Adenoviral Vaccines versus an Autogenous Vaccine

Alberta Turkey Producers The Alberta Turkey Producers have recently compiled a list of strategic priorities for 2013.

Major focuses for the upcoming year include:       

mandatory implementation of the Turkey Farmers of Canada On-Farm Food Safety Program for Alberta registered growers completion of the provincial regulatory review process ensuring that Alberta production aligns with National Allocation, transitioning production planning from a processors based process to a board based initiative Increased communication and education to producers on conditional allocations, the allocation process and on-farm programming Continuing to promote a positive image of the supply management system Setting the course and creating the environment for industry growth and development Continued education directed toward consumers and Albertans on Alberta Turkey

The Alberta Turkey Producers value investment in the future and the maintenance of a sustainable and healthy agricultural industry. Organizations such as the Poultry Research Centre encourage the continuation of poultry research programs which lead to knowledge transfer encouraging industry growth, innovation and the adoption of best practices. Continued investment in students, research and innovation is important to the success of our industry.

Burnbrae Farms Burnbrae Farms is a very progressive family farming business that seeks to meet the needs of its customers through innovation. Burnbrae Farms has a mandate to improve the sustainability of its day to day operations. At Burnbrae Farms we consider research to be essential in meeting these objectives. We do research on farm as well as avidly supporting research at the university level.

Burnbrae Farms is excited to contribute to and be part of such a collaborative team as that at the University of Alberta Poultry Research Centre. A strong poultry research centre is central to attracting research funding, implementing solid research programs and educating and training highly qualified personnel to work in our industry. The benefits of being a stakeholder are above and beyond the aforementioned. The working relationships that develop from shared communication and connections allows for knowledge sharing and strategy development between stakeholders.

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The PRC Annual Report 2013

Research is a critical component of our industry. It fuels future technologies, solves real time industry issues and trains future poultry industry personnel. Our industry is privileged to have a strong poultry research component. We need to support it.

Egg Farmers of Alberta Hen Housing continues to be a critical issue for the egg industry. On February 26, 2013, at its AGM, a motion was passed giving direction to the Egg Farmers of Alberta’s Board of Directors to enact a policy that would begin to transition the Alberta egg industry away from conventional cages. Representatives from Egg Farmers of Alberta attended the Calgary CO-OP AGM on March 13, 2013, when a non-binding motion was passed (59% of votes for) that encouraged the Calgary CO-OP Board of Directors to investigate the feasibility of phasing-out the sourcing of eggs from conventional cages over a 5-year period. While it remains to be seen how the Calgary CO-OP Board will decide to proceed it is clear that this issue is continuing to gain momentum. To help producers who are re-caging or building new barns decide on what hen housing system to use, we have developed a hen housing guide which details the history of the hen housing issues, outlines the different options for hen housing and provides some decision making tools to go through to help producers choose the system that makes the most sense for their farm. We have asked all producers who are thinking of retooling to meet with a representative from EFA before making their decision. In addition, we are working on continuing to document and analyze cost of production and start-up capital costs for alternative systems, and on developing a resource to help producers who have already switched to enriched better manage their first flocks in light of the changes in hen management required.

Corporate sustainability initiatives have grown in number, scope, and size in recent years and most consumers today are thinking about the environmental impact of the products they purchase. In 2012, we heard from producers about valuable roles for EFA in administering an environmental program with a strong theme being that producers believe an environmental program should be developed in order to maintain and improve consumer confidence, provide information on best management practices, and ensure the industry is prepared to meet new information requirements from retailers. Much like when EFA has taken the initiative in animal care programs, the development of a credible environmental program based on science and adapted to producer needs is a way to help ensure producers maintain control of their own destiny. Throughout 2012 and 2013, EFA has been working with the ARD Environmental Farm Plan group to develop and environmental education program that will be launched in early 2014.

EFA’s New Entrant Program (NEP) was developed and launched in 2012. It is vital to the longterm sustainability of the egg industry that new people and new investments are encouraged.

The PRC Annual Report 2013

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The NEP was developed in order to assist individuals and families who want to own and operate an egg farm, by issuing a portion of the newly allocated egg quota to successful applicants, thus alleviating some of a producer’s start-up costs. In 2012, EFA transitioned quota that was previously allocated for the Market Development Leasing Program (MDLP) for new organic producers, into the NEP. To facilitate the transition, NEP quota lots of 1,000 birds were allocated to existing MDLP producers. As a result, EFA has welcomed its first 5 producers under the NEP. Being part of the PRC is a great way to ensure constant communication between industry and researchers regarding both overall research and development needs as well as specific challenges and opportunities. The PRC supports the egg industry with scientific input on production issues that we can take directly to our producers. Furthermore, the PRC provides us with timely access to experts when unique challenges arise. The PRC also acts as a separate and objective voice for the industry on issues related to food safety and animal care which is extremely valuable in times of opposition to the use of scientifically validated practices. In addition, by working together, we have an opportunity to derive economic value from the application of technologies that can broaden our markets and result in more efficient use of byproducts.

Egg Farmers of Canada In 2012, Egg Farmers of Canada celebrated the 40th anniversary of national egg supply management. We can proudly reflect on 40 years of innovation that have seen the introduction of new egg products offered to consumers, world-class on-farm food safety and animal care programs, a national traceability standard, and an expanding research program. EFC takes a proactive strategy to communicate the value of supply management to our elected officials and to the public. We do this through a variety of activities including our annual Parliament Hill Breakfast, egg farm tours for MPs and Senators, through media relations and by writing letters, and online ad campaigns.

Egg Farmers of Canada is proud to support research that invests in egg farmers’ livelihoods, improves hens’ well-being, contributes to finding environmental solutions, and advances the health and nutritional benefits that eggs provide. Research at EFC brings together creative and collaborative solutions where desirable outcomes for both farmers and consumers are needed.

The research partnership with PRC helps to build on the body of scientific knowledge and expertise that the egg industry relies on in their efforts for continuous improvement by contributing to today’s research excellence and the next generation of poultry scientists and industry experts.

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The PRC Annual Report 2013

Maple Leaf Poultry research is vital to all stakeholders in the Canadian poultry industry. Discoveries which improve production efficiencies, make advancements in on-farm food safety, biosecurity and disease control, improve poultry welfare and food safety, and develop new and innovative consumer products benefit the entire poultry supply chain.

As a poultry processing company, Maple Leaf Foods and all Canadian poultry processors (represented largely by the Canadian Poultry and Egg Processors Council) recognize the value of investing in research through the University of Alberta’s Poultry Research Centre. Poultry science and its impact on the Canadian poultry industry are an integral part of the long term health of the sector. The PRC is well positioned both in its strong research capabilities and the ability to ensure transfer of research findings to industry stakeholders.

University of Alberta As Chair of the department of Agricultural, Food and Nutritional Science (AFNS), I am glad to contribute to the Annual Report of the Poultry Research Centre. Without hesitation, we can assert that there are many areas of success for AFNS and the PRC and its partners including the renewal of the agreement to support the PRC for another 5 years.

The PRC is an important Centre for the University because it connects the University to the broader communities and partners including the various segments of the Poultry industry and customers. We are indeed, honored to have a world class Centre, whose accomplishments will over time, be felt all across the world. Indeed, our partnerships with the poultry industries and our funders have helped to keep our research focused, relevant and applicable and thus have helped us to contribute to the economic, social, and environmental sustainability of the industry. In addition, these partnerships have helped to link our students to jobs/careers in fields that they have been trained for.

With the strong support from our partners and funders, we wish to extend our heartfelt appreciation to each member of the PRC for making the PRC a place for innovation. I know that with such support, we will continue to make the research program at PRC the envy of the world.

The PRC Annual Report 2013

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Awards Congratulations to our hard working faculty and students who have earned recognition for excellence in teaching and research.

Faculty Awards 1. Leluo Guan 2. Aman Ullah 3. Martin Zuidhof

ALES Teaching Wall of Fame Rising Star in Global Health award (Grand Challenges Canada) ALES Teaching Wall of Fame

Graduate Student Awards 1.

2.

3.

4.

5.

6.

7.

8.

9.

10.

11.

12.

Carlos Lozano Kim Ton Thania Moraes Kim Ton Airell DesLauriers Thania Moraes Yussef O. Esparza Nandika Bandara Nandika Bandara Forough Jahandideh Jiandong Ren Sahar Navidghasemizad

13. Chamila Nimalaratne 14. Kaustav Majumder 15. Kaustav Majumder 16.

17.

18.

19.

20.

21.

22.

Ali Akabari Cibele Torres Cibele Torres Airell DesLauriers Airell DesLauriers Daylin Hincampie Melissa Johnson

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(Zuidhof) AFNS differential tuition award (Zuidhof) WPC student program (Zuidhof) Student program (WPC 2012) (Zuidhof) Student program (WPC 2012) (Zuidhof) Graduate Student Teaching Award (AFNS) (Zuidhof) GSA Professional Development Grant (Wu) Becas Chile Scholarship (Wu) AFNS Differential Tuition Award (Wu) J Macgregor Smith Graduate Scholarship (Wu) AFNS Differential Tuition Award (Wu) AFNS Graduate Research Assistantship Fund (GRAF) Fall 2012 (Wu) 1st place winner of 2012 George F. Stewart International Research Paper Competition of the Institute of Food Technologists (Wu) AFNS Graduate Research Assistantship Fund tuition (Wu) Don and Mary Ann Copeland Award (Wu) 1st place winner poster competition of the Nutraceutical and Functional of the Institute of Food Technologists (Wu) University of Alberta Doctoral Recruitment Scholarship (Korver) Lloyd Johnson Graduate Scholarship (Korver) Certificate of Excellence, Poultry Science Association (Zuidhof) Graduate Student Teaching Award (ALES) (Zuidhof) Graduate Student Teaching Award (FGSR) (Betti) AFNS differential tuition award (Korver) Alberta Ingenuity scholarship

The PRC Annual Report 2013

Graduations Congratulations to the following 10 students who earned their degrees in the current year 2012-2013.

Graduate 1. Huiting Huang

Supervisor Steiner

Degree Topic MSc Quality based poultry pricing

2.

Kathleen Vail

McMullen

MSc

Filamentation of Listeria monocytogenes

3.

Mojtaba Yegani

Korver

PhD

Variation in feedstuff quality for broiler chickens

4.

Melissa Johnson

Korver

PhD

Breeder antioxidants and broiler immunity

5.

Cibele Torres

Korver

PhD

Organic trace minerals in breeder diets and embryo bone growth

6.

Thania Moraes

Zuidhof

MSc

Maternal nutrition and broiler performance

7.

Jiandong Ren

Wu

MSc

Phosvitin extraction from yolk

8.

He Nan Wang

Wu/ Betti

MSc

Spent hen protein extraction

9.

Justina Zhang

Wu

MSc

Fermentation to reduce egg white allergenicity

10. Sahar Navidghasemizad

Wu/ Temelli PhD

Extraction of egg phospholipids

The PRC Annual Report 2013

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Research Highlights

Research Impact: Beneficial application of research to achieve social, economic, environmental and/or cultural outcomes We asked PRC scientists to summarize the impact of their research. This is what they said:

Eduardo Beltranena This year, we moved on from conducting camelina feeding studies with broilers to egg layers.

Camelina or ‘false flax’ is an oilseed with great potential to diversify vegetable oil production in the Prairie Provinces. It won’t directly compete with canola, but complement it. Camelina will be a bio-industrial crop whose oil will be primarily targeted to biojet fuel production (Sustainable Aviation Fuel Users Group, SAFUG) and bio-industrial oil applications like biofilm, bioplastics, aliphatic polyesters, etc. It will be grown primarily in marginal land in Brown or Dark Brown soils in southeast AB, southern SK and southwest MB where its disease toughness and thermotolerance could add >1.5M acres of oilseed production. Our interest for poultry feeding is that the meal after oil expelling has 12 – 20% residual oil, which is rich in omega-3 fatty acids.

But unlike flax, it is high in vitamin E, a naturally occurring antioxidant that prevents its oil from going rancid. Fed to broilers and egg layers, it has the potential to not only enrich meat and eggs with omega-3 fatty acids, but the residual oil also provides extra calories at a fraction of the cost compared with adding tallow or canola oil to poultry diets to increase dietary energy content. However, feeding camelina meal to poultry and livestock is not currently approved in Canada. The trials we are conducting aim to overcome this limitation and have camelina meal listed in Schedule IV of the Feed Act. Bioindustries utilizing camelina oil must bury the meal, as it is not approved for animal feeding, thus wasting ~60% of the seed. Once we have it approved for animal feeding, bioindustries will recover value far beyond seed cost. We confirmed omega3 fatty acid enrichment of broiler thigh and breast meat last year. This year, we have confirmed omega-3 enrichment of eggs from both white and brown layers feeding increasing levels of camelina meal inclusion. Along our research with camelina meal, we have also evaluated a lower-fibre, yellow-seeded canola meal (Brassica juncea) vs. conventional dark-seeded canola meal (B. napus) for feeding layers. We determined that either solvent-extracted or expellerpressed, the meal from these 2 canola cultivars can be fed to layers at high dietary inclusion levels (up to 20%) without impacting layer performance or egg quality.

Mirko Betti Maximizing the value of poultry meat processing by-products: Dr. Betti has developed a method of preparation of functionalized glycated and glycosylated collagen and gelatine peptides from bovine hides and poultry processing by-products. His research on functionalized proteins/peptides has resulted in three patent applications filed in Page | 18

The PRC Annual Report 2013

September 2012. The technology has brought significant attention from the industry and Mirko has hosted two visits from international companies, Tessenderlo Group regarding collaboration.

March 11 - 17th is the National Salt Week and the Ministry of Wellness wants to bring awareness of this issue to the Legislature. Dr. Mirko Betti was invited to speak to Ministers in the Legislature including the Honourable Minister Stephen Khan, Enterprise and Advance Education and Honourable, Minister Verlyn Olson, Agriculture and Rural Development, and the Associate Minister of Wellness, Dave Rodney, about his research in flavour enhancer peptides obtained from meat by-products to replace salt in processed foods.

Valerie Carney Although our final Best Management Practice project recommendations have not yet been released to the industry we have had an impact through working directly with producers onfarm and through workshops. We provided producers with standardized data collection materials and protocols, which for some producers was a major change in practice. They are able to quickly analyze their own data and make informed management decisions. Standardized data collection allows experts from breeding companies, the feed industry, hatcheries, and veterinarians to more effectively to identify issues and areas of improvement. Management of a hatching egg farm is a complex operation that requires effective data management and communication between producers, hatcheries and service personnel to make well informed decisions. This project has enabled this communication to happen in a more effective, efficient and cooperative manner.

Doug Korver My group continues to work primarily in applied poultry nutrition, and although the proportion of time spent working on projects specifically related to each of the four poultry sectors varies, in many cases what we learn from our projects has relevance to multiple sectors. In the past year, I have had several projects specifically related to broiler breeder nutrient transfer to the chick, a broiler strain trial with a primary chicken breeding company, a field trial with the Egg Farmers of Alberta, and a broiler hatching egg incubation trial. Much of my nutrition and ingredient work with broiler chickens is also relevant to turkey production. I continue to work closely with international manufacturers and suppliers of feed ingredients including enzymes, vitamins and pigments. In the past year, my research maintained momentum in providing solutions to practical questions in the Alberta poultry industry. My research into commercially available, or near commercial feed ingredients and supplements means that Alberta poultry producers and nutritionists can make more confident decisions about supplementing their poultry rations to maintain high levels of productivity.

Lynn McMullen Food safety continues to be a challenge for anyone working in the food processing industry. The interest in receiving advanced training in food safety is spiraling and I have been able to recruit some excellent graduate students who will be will prepared to work in the industry to help reduce food safety risks. Understanding how foodborne pathogens grow and survive in food The PRC Annual Report 2013

Page | 19

products with complex microbiota is key to ensuring we can develop appropriate and effective interventions to reduce the risks associated with foods. For example, Listeria monocytogenes reacts differently when grown on ready-to-eat meats with a low salt content. It will grow faster if a competitive microbiota is not present on the products. Listeria also will form long, filamentous cells on some products. This physiological change in cell length could impact enumeration of L. monocytogenes on meat products. We have also worked on evaluation of high pressure processes to reduce numbers of L. monocytogenes on poultry products. This industry driven project provided the processor with data needed to develop parameters for industrial processing and utilized our unique facilities in the Meat Safety and Processing Research Centre where we can conduct experiments in foods with foodborne pathogens.

Aman Ullah With continuous and sustainable growth of poultry industry and an ever increasing demand for poultry consumption is leading to an oversupply of byproducts. Efficient utilization of byproducts is a challenge but extremely important for the profitability of the poultry industry.

Our major focus is on finding alternative and innovative ways to utilize them such as water purification filters from feathers to remove toxic metals and develop nano-structured biodegradable food and non-food packaging with large social, economic, and environmental benefits.

Wendy Wismer Dr. Wismer continued her research program in sensory and consumer science, including a project in the poultry setting. She supervised a senior food science undergraduate project to gauge consumer acceptance of eggs from Dr. Beltranena’s study evaluating the incorporation of camelina meal in the diet of laying hens. The undergraduate project will serve as the foundation for an expanded project in summer 2013.

Jianping Wu Bioactive peptides are protein fragments that have a wide range of applications in functional food and nutraceutical, cosmetic, food and nutrition industries. Wu’s bioactive peptide research program continues to understand the potential of egg proteins and low value meat proteins. The 2nd stream of research is to utilize underutilized poultry byproducts such as feather for plastics and spent hens for glue. Through egg for health research, his program is to provide evidence-based knowledge to promote table egg consumption and thus to support a sustainably growing egg industry in Alberta and in Canada.

Egg Research Attracts Interest from Major Egg Processing Company Egg research and discovery has attracted interest of the world’s largest egg processing company, Michael Foods Group, Inc., based in Minnetonka, MN, USA. Following a successful Banff Egg Forum in March 2012, Dr. Jonathan Merkle (VP-R&D) and Dr. Si-Quan Li (Fellow Scientist) visited U of A to seek opportunities of research collaboration and commercialization.

We have defined area of mutual interest and a research proposal has been submitted for funding support.

Egg Research Connected to the World Page | 20

The PRC Annual Report 2013

Dr. Jianping Wu was invited by Egg´s Producers Association of Chile for a week to talk about the value-added research program and our recent discovery of new bioactive egg components such as new egg antioxidants and antihypertensive activity that could benefit for preventing cardiovascular diseases. The goals of the trip were to present value-added egg opportunities to the industry and to educate the professionals and the public about the misperception of egg consumption over health.

Peptides for Cardiovascular Diseases Egg ovotransferrin derived antihypertensive peptides proved to be bioavailable in spontaneously hypertensive rat (SHR), a well-established model of human essential hypertension. The antihypertensive peptide IRW reduced mean blood pressure by ~10 mmHg at a dose as low as of 3 mg/kg body weight and ~40 mmHg at the dose of 15 mg/kg body weight, respectively, compared to untreated SHRs. In addition, egg antihypertensive peptides also showed anti-inflammatory activity and improved endothelial dysfunction in vivo; inflammation and endothelial dysfunction are two identified risk factors for many types of chronic diseases, such as diabetes and cardiovascular diseases. Cardiovascular diseases remain the number one cause of death in the world; egg protein derived antihypertensive peptides might find applications as functional food or nutraceutical ingredient in the prevention and management of hypertension and other cardiovascular diseases.

Peptides for bone and dental health Egg yolk contains a unique phosphorylated protein, phosvitin. Our research has developed new methods of phosvitin extraction and phosvitin peptide preparation from egg yolk. Casein phosphopeptides are now used clinically in the treatment of dental caries and other hypomineralized conditions, including osteoporosis; the potential of phosvitin phosphopeptides yet to be explored. According to Health Canada, an estimated 1.4 million Canadians are believed to have osteoporosis, one in four women and one in eight men over 50 years of age, causing billions of dollars expenditure in health care.

Peptides could be the grandma’s penicillin?

Ongoing research on spent hen derived peptides focused mainly on immunomodulatory peptides based on the belief of “chicken soup as grandma’s penicillin”. Our progress indeed showed chicken proteins are good source of bioactive peptides; peptides prepared from both muscle proteins and collagen showed immunomodulatory properties, antioxidant and antihypertensive activities. Lilydale is supportive to our research and is encouraging us to apply the technology to other low value poultry byproducts; we are continuing to explore the opportunity with Lilydale.

Glue from spent hens and poultry byproducts We have just completed the phase one study of preparing adhesives from spent hen proteins in 2012. Waste and byproducts from the poultry industry in Alberta represents approximately $45 million economic potential, $14.4 M for the spent hen alone, $26.7M for the chicken byproducts and $4.2M for the turkey byproducts. Working with Tec-Edmonton, we have partnered with a multinational specialty chemical company to sign a Material Transfer Agreement to assess the glue application in the real industry setting. Our further works are to The PRC Annual Report 2013

Page | 21

mature the technology by demonstrating its scalability and applications as well as to develop strategy for commercialization with industry partner. Developing protein adhesives from renewable sources such as spent hens will not improve profitability of the producers, but also contribute to grow Alberta’s bioeconomy in an environmentally sustainable manner.

Martin Zuidhof Develop transformative feeding systems to address major production, uniformity and efficiency challenges in hatching egg, broiler, turkey, and egg sectors: Precision Broiler Breeder Feeding System The purpose of the project is to develop an innovative pre-commercial Precision Broiler Breeder Feeding System (PBBFS). A feeding station has been designed to control individual bird feed intake by precisely matching individual BW measurements to BW targets. Evaluation of a prototype system began in February, 2013. The prototype dispenses small meals to each bird in the flock multiple times each day - delivering the right amount of feed to the right bird at the right time. In contrast to traditional management of feed restriction, which involves daily or every-other-day feeding, broiler breeder pullets and hens will now be able to "graze" throughout the day which will improve their overall welfare and stabilize their metabolism (preventing the inefficiency of storing and mobilizing nutrients). The project will evaluate alternative BW curves to improve welfare, and profitability through flock uniformity, egg production, fertility and hatchability. The outcomes of the project include providing information needed to implement the system on a commercial scale.

Our collaborating engineers from Xanantec Technologies Inc. have been working closely with the project team on designing the system. Dr. Koos van Middelkoop from The Netherlands is also a member of our study team and met in person with the project team in September, 2012.

He continues to be involved, remotely offering input.

Turkey Quality and Welfare This research project is led by Drs. Irene Wenger and Martin Zuidhof (U of A), with involvement from Alberta Turkey Producers, ARD, and Dr. Tom Inglis (Poultry Health Services). The technology transfer component is being led by Dr. Valerie Carney (ARD). A turkey processor in Alberta expressed concerns about a higher incidence of Airsacculitis and downgrades among heavy tom turkeys raised in Alberta. In response to these concerns, the project team initiated an intensive field study with 10 Alberta turkey flocks. The purpose of the project is to assist Alberta Turkey Producers and processors to identify where potential problems lie in the production and processing of heavy toms. Solutions can then be implemented, and where possible targeted education and extension material can be developed to support required changes in industry practices to improve bird welfare and turkey product quality.

Data was collected at turkey poult placements. In addition, relative humidity/temperature and ammonia sensors were mounted in each barn for recording environmental conditions throughout the entire brooding and growing cycle of each of 2 flocks from each participating farm. Data on performance and quality was also collected at the farm during loadouts and at the processing plant. Analysis is currently underway.

Page | 22

The PRC Annual Report 2013

Organizational Structure Board Board Member Helen Anne Hudson, Chair

Representing National Industry

Term end date 2016

Wes Johnson, Vice chair

Government of Alberta

2014

Martin Zuidhof

Academic Leader

2014

Erasmus Okine

AFNS

2015

Jianping Wu, ex-officio

Researchers

2015

Susan Novak

Funders

2016

Karen Kirkwood

Chicken Industry

2015

Jenna Griffin (Latanville)

Egg Industry

2013

Susan Schafers

Independent

2016

Leonard Waldner

Turkey Industry

2016

Ashley Rietveld

Hatching Egg Industry

2013

Sunny Mak

Processing

2016

Tim Nelson

National Industry

2015

Reg Cliche

Processing

2016

The PRC Annual Report 2013

Page | 23

PRC Operations Personnel Staff Member

Affiliation

Role

Martin Zuidhof

University of Alberta

Academic Leader

Valerie Carney

Alberta Agriculture and Rural Development Technology Transfer

Doug Korver

University of Alberta

Teaching and Learning

Agnes Kulinski

University of Alberta

Business Development

Laurie Heidebrecht University of Alberta

Administrative Support

Lyle Bouvier

University of Alberta

Poultry Unit Manager

Nigel Davidson

University of Alberta

Poultry Unit Technician

Gilles Hinse

University of Alberta

Poultry Unit Technician

Chris Ouellette

University of Alberta

Instrumentation Technician

Shawn Rankin

University of Alberta

Poultry Unit Technician

Page | 24

The PRC Annual Report 2013

Researchers Name (N=13)

Position (% FTE, if less than 100%)

Student1

Specialty

Grad U/G Eduardo Beltranena Mirko Betti

Research Scientist, ARD; Adjunct Professor (33%) Associate Professor

Chemistry/ Biochemistry of muscle foods Applied poultry research

Post doc

2

9

Research & extension specialist, ARD Professor Associate Professor

Agricultural marketing and business Metagenomics

Jenny Fricke

Veterinarian

Poultry Health

Douglas Korver Lynn McMullen Robert Renema Bodo Steiner

Professor

Poultry nutrition

5

Professor (10%)

Food microbiology

2

Assistant Professor (25%) Professor (10%)

Poultry physiology and valueadded products Marketing of poultry products

Aman Ullah

Assistant Professor

Poultry by-products

Wendy Wismer Jianping Wu

Associate Professor (10%) Associate Professor

Sensory and consumer science High value egg utilization

14

Martin Zuidhof

Associate Professor

Poultry systems

5

Research Associate

Visiting Scholar

Visiting

Monogastric feeds and feeding

Valerie Carney Ellen Goddard Leluo Guan

2 1

1

2

1

1

1 1

0. 5

3

0. 5

2

1

4

1

1

1

1 1

36 1

Technician

2

3

3

1 4

6

13

Students co-supervised by two PRC researchers are counted as 0. 5 The PRC Annual Report 2012

Page | 25

1 2

8

6

1

Graduate Students Graduate Student 1. Ali Akbari

Supervisor Wu

Degree PhD

General Research Focus Nanoencapsulation of bioactivities

2. Mohannad Badawi

Betti

MSc

High pressure processing of gelatin

3. Nandika Bandara

Wu

PhD

Adhesive from agriculture waste and by-product

4. Misaki Cho

Korver

PhD

5. Airell DesLauriers 6. Lihui Du

Zuidhof Betti

MSc PhD

25-OH vitamin D3 and canthaxanthin in breeder diets Broiler breeder precision feeding Anti-freezing proteins/peptides

7.

8.

9.

10.

11.

12.

13.

Wu Korver Wu Betti Betti Steiner Wu

PhD MSc PhD PhD PhD MSc MSc

Plastics from poultry feather Genomic effects of 25-OH vitamin D3 in broilers Structure and function of bioactive peptides Salty and kokumi peptides from food proteins Glycation/glycosylation of isolated muscle proteins Quality based poultry pricing Bioactive peptides for cardiovascular diseases

PhD PhD

Breeder antioxidants and broiler immunity High Pressure resistant pathogens in poultry meat

16. Christine Liu 17. Carlos Lozano

Korver Gaenzle/ Betti McMullen Zuidhof

PhD MSc

Bacteriology and food safety Broiler nutrition

18. Kaustav Majumder 19. Daylin Martinez 20. Thania Moraes

Wu Betti Zuidhof

PhD MSc MSc

Ovotransferrin derived bioactive peptides Gelatin modification/antimicrobial peptides Maternal nutrition and broiler performance

21. Sandeep Nain

Betti

PhD

Omega-3 enriched poultry products

22. Sandeep Nain 23. Sahar Navidghasemizad 24. Liyana Nimalaratne

Betti Wu/ Temelli

PhD PhD

Omega-3 enriched poultry products Phospholipid extraction from egg yolk

Wu/ Schieber Zuidhof Wu Wu Wu Zuidhof Korver

PhD

Antioxidants in eggs

MSc MSc PhD PhD MSc PhD

Renema/ Guan McMullen

MSc

Broiler breeder management Phosvitin extraction from yolk Phosvitin derived bioactive peptides Ovomucin structure and function Antibiotic free broiler production Organic trace minerals in breeder diets and embryo bone growth Broiler metagenomics

MSc

Filamentation of Listeria monoctygoenes

Yussef Esaparza Saman Fatemi Yuchen Gu Pui Khoon Hong Yuliya Hrynets Huiting Huang Forough Jahandideh 14. Melissa Johnson 15. Yang Liu

25.

26.

27.

28.

29.

30.

Dulal Paul Jiandong Ren Jiandong Ren Xiaohong Sun Kim Ton Cibele Torres

31. Soledad Urrutia 32. Kathleen Vail

The PRC Annual Report 2012

Page | 26

Graduate Student 33. He Nan Wang

Supervisor Wu

Degree MSc

34. He Nan Wang 35. Mojtaba Yegani 36. Wenlin Yu

Betti Korver Wu

PhD PhD PhD

General Research Focus Spent hen protein extraction and adhesive preparation Functionalization of Poultry/Bovine collagen Variation in feedstuff quality for broiler chickens Bioactive peptides from spent hens

Visiting Students and Scholars Name 37. Lenka DiblĂ­kovĂĄ 38. Jun Fang 39. Davide Gottardi 40. Shirin Honarbakhsh 41. Rossawan Intarasirisawat 42. Bob Lambrechts 43. Juan You

Program PhD student exchange (VSCHT Prague) Visiting Scholar (China) University of Bologna (Italy) Visiting student (Iran) Visiting PhD student (Thailand) Visiting student (Netherlands) Visiting PhD Student (China)

Team Betti Wu Betti Renema Wu Carney Wu

Technical Support Name 1. Alexandra Acero Lopez 2. Dustin Banks 3. Michelle Beveridge 4. Breanne Chmilar 5. Ken Fahner 6. Erica Holm 7. Monika Kozelkova 8. Ross Lowe 9. Kamila Moquin 10. Thania Moraes 11. Kerry Nadeau 12. Marina Offengenden 13. Sareh Panahi 14. Arisha Seeras 15. Dharma Shrestha 16. Patrick Ward 17. Jordana Williams

Title Research Technician U/G Research Assistant U/G Research Assistant Research Technician MSPRU Manager Research Technician U/G Research Assistant Research Technician Research Technician Research Technician Research Technician Research Technician Research Technician Research Technician Research Assistant Research Technician Laboratory Technician

Team Wu Carney McMullen Beltranena McMullen Carney McMullen McMullen McMullen Zuidhof Korver Wu Wu McMullen Beltranena McMullen Korver

The PRC Annual Report 2013

Page | 27

Post-Doctoral Fellows Name 1. Satyanarayana Bejiani 2. Jacob Hamidu 3. Zied Khiari 4. M. A. Khosa 5. Petr Miller 6. Satyanarayana Bejiani 7. Chanchan Wang 8. Jine Yi

Team Betti Korver Betti Ullah McMullen Wu Wu Wu

Subject Valorization of Poultry processing by-products Embryology Valorization of Poultry processing by-products Poultry by-products Food microbiology Value added egg science Value added egg science Value added egg science

Research Associates Name 1. Maurice Ndagijimana 2. Matt Oryschak 3. Ali Pishnamazi 4. Jennifer Saunders-Blades 5. Brenda Schneider 6. Irene Wenger

Page | 28

The PRC Annual Report 2013

Title Research Associate Research Associate Research Associate Research Associate Research Associate Research Scientist

Team Betti Beltranena Zuidhof Korver Carney Zuidhof

Research Projects ($3,228,414)

Research Projects

(Received in 2012-2013)

$3,228,414

Bacteriology / Food Safety

$408,198

Granting Body

Abbreviated Title

ALMA 2010F131R

Improving the safety of low salt meats

ALMA/AI-BIO 2010F149R

Impact of micocin on the transfer of L. monocytogenes in sliced deli meats

ALMA Maple Leaf Foods NSERC Discovery ALMA/AB-BIO

High pressure processing to control L. monocytogenes in RTE poultry products Role of bacteriocins in food safety and quality Micocin to control Listeria in delis

Amount received in current year 133,400

Applicant(s)

Planned Duration

L. McMullen (PI) P. J. Shand D. Korber L. M. McMullen (PI) D. Carlson (CanBiocin) M. E. Stiles (CanBiocin) D. C. Smith (Griffith Laboratories) L. M. McMullen (PI)

20102014

$520,375

20122014

$317,000

20122013

$95,992

$31,000

L. M. McMullen

2012 2017

$155,000

$63,300

L. M. McMullen, D. Carlson, M. E. Stiles, D. C. Smith

158,700

$21,798

Feed and Nutrition

Total value of grant

$377,900

Granting Body

Abbreviated Title

DSM Nutr.

Prod.

Agric. Funding

Mechanisms of increased breast meat yield in broilers fed 25-OH Vitamin D3

Amount received in current year $246,517

Applicant(s)

Planned Duration

D. Korver C. Fitzsimmons M. Zuidhof

2012

The PRC Annual Report 2012

Page | 29

Total value of grant $246,517

Consortium ACP U of A Aviagen, Inc. Agriculture Development Fund, SK AAFC, Canola Council

Aviagen Strain Trial

$30,636

Safety and efficacy of feeding camelina meal to egg laying hens

67,747

High inclusion levels of regular and high energy canola meal in swine and poultry diets

33,000

D. Korver M. Zuidhof E. Beltranena (AB), C. Christensen, T. Scott, A. van Kessel (SK) E. Beltranena (poultry and swine), R. Zijlstra (swine)

2012

$30,636

20122014

208,391

20102013

100,000

Management and Physiology Granting Body

Abbreviated Title

ALMA Egg Farmers of Alberta Alberta Agriculture & Rural Development U of A ALMA 2010R069

Management strategies to increase Alberta egg farm revenue

GF6090-60921 Page | 30

$459,361 Amount received in current year $57,119

Applicant(s)

Planned Duration

D. Korver V. Carney B. Schneider R. Renema

20102013

$243,878

Improved hatchability via data handling best management practices

$181,121

20092012

$181,121

Improved hatchability via data handling best management practices

$20,000

V. Carney B. Schneider N. Robinson R. Renema M. Zuidhof F. Robinson V. Carney B. Schneider

20092012

$66,050

The PRC Annual Report 2013

Total value of grant

ALMA 2010R069

Improved hatchability via data handling best management practices

$181,121

GF6090-60921

Improved hatchability via data handling best management practices

$20,000

N. Robinson R. Renema M. Zuidhof F. Robinson V. Carney B. Schneider N. Robinson R. Renema M. Zuidhof F. Robinson V. Carney B. Schneider N. Robinson R. Renema M. Zuidhof F. Robinson

20092012

$181,121

20092012

$66,050

Meat and Egg Products and Processes

$1,327,034

Granting Body

Abbreviated Title

AI-Bio (sub-grant from Bioactive Oils Program)

Proof of Concept: Using SDA Flax to increase long-chain ω-3 PUFA in table eggs

Grand Challenges Canada (GCC)

Filters from poultry feathers for removal of Arsenic from drinking water in developing countries.

Isolated proteins from low value poultry meat: potential applications

ALMA/AIBio/ACP

Amount received in current year $22,879

$113,000

$94,333

Applicant(s)

Planned Duration

R. Weselake (main PI) D. Korver (sub-grant PI) R. Renema M. Zuidhof M. Betti Ullah, A. Bajaj, D. R.

2012

Betti (PI), Pietrasik, Gerlat, Erin

2010 2013

The PRC Annual Report 2013

Page | 31

Total value of grant $22,879

$113,000

283,000

ALMA/RossDown Natural Farms/ACP ALM/AIBio/Maple Leaf Foods/ACP ALMA/ Maple Leaf Foods/ACP

Preparation and characterization of high quality gelatin from different poultry sources Innovative functional ingredients from poultry biomass: salty and “kokumi� peptides Innovative functional ingredients from poultry biomass: extraction and isolation of chondroitin sulfate ALMA Targeted design of novel antimicrobial agents for pathogen intervention on meat NSERC DG Antioxidant peptides from egg proteins Canadian Poultry Protein-based biomaterials from Research Council spent hens Agriculture Utilization of Protein-Containing Funding Agriculture Waste and By-Products Consortium for Adhesive Development Agriculture and Antihypertensive Activity of Laying Agri-Food Council Hen Eggs (CAAP program) Agriculture and Health-promoting components in Agri-Food Council Laying Hen Eggs (CAAP program) Agriculture and Functional egg bioactive peptides: Agri-Food Council scale-up processing and in vivo (CAAP program) efficacy Alberta Livestock Learning from grandma: developing Meat Agency valuable functional food ingredients (ALMA) from spent hens Page | 32

The PRC Annual Report 2013

$38,333

Betti (PI), Pietrasik

20112014

$115,000

165,000

Betti (PI), Gaenzle, Schieber, Ndagijimana

20112013

$330,000

$110,000

Betti (PI), Pietrasik

20112013

$220,000

$150,000

Gaenzle (PI), Betti, McMullen

20122014

$300,000

$25,180

Wu, J.

$125,900

$20,000

Wu, J and Betti, M

$136,504

Wu, J. Betti, M. Che, L. Qi, H. and Carney, V.

20082013 20092012 20092012

$103,582

Wu, J. and Susan Jacobs

20102014

$439,757

$76,270

Wu, J.

20112013

$185,661

122,7. 5

Wu, J., Davidge, S. and Susan Jacobs

20112014

$354,315

$72,519

Wu, J. and Field, C.

20112013

$146

$60,000 $475,136

NSERC RT

ARCHITECT i2000SR System

$131,679

NSERC RT

Microplate reader, a necessary tool for functional genomics and proteomics studies in animal, microbial and plant systems Determination of the effect of processing and storage conditions on the stability and antioxidant activity of carotenoids in egg yolk

$38,755

Food and Health Innovation Initiative (Vitamin Fund)

$29,000

Field, C. (PI), Wu, J. and other four Guan, L. (PI), Wu, J. and other 8

20122013 20122013

$131,679

Wu, J.

20122013

$29,000

Metabolism and Reproduction

$266,336

Granting Body

Abbreviated Title

NSERC

Bone metabolism in fast and slowgrowing broilers Maternal canthaxanthin and 25-OH $138,967 vitamin D3 effects on chick growth performance, meat yield, and susceptibility to inflammation Effect of broiler breeder age on $102,369 embryo metabolism and chick innate immune function and bone development

ALMA, DSM Nutritional Products, U of A

ALMA, ACP, Canadian Poultry Research Council, Poultry Industry Council, Poultry Research Centre, U of A

$38,755

Amount received in current year $25,000

Applicant(s)

Planned Duration

D. Korver

2012

$25,000

D. Korver, J. Saunders-Blades, R. Renema

20122014

$409,154

D. Korver

20122013

$196,969

Poultry Systems

Total value of grant

$343,585

Granting Body

Abbreviated Title

ACP 2011F121R

Precision Broiler Breeder Feeding

Amount received in current year 2,500

Applicant(s)

Planned Duration

M. Zuidhof

2011-

The PRC Annual Report 2013

Page | 33

Total value of grant 7,500

System AI-Bio Precision Broiler Breeder Feeding 2011F121R System ALMA Precision Broiler Breeder Feeding 2011F121R System AHEP Precision Broiler Breeder Feeding System CHEP Precision Broiler Breeder Feeding System Poultry Industry Precision Broiler Breeder Feeding Council System Ag & Food Precision Broiler Breeder Feeding Council (CAAP System through AHEP)

8,750

C. Bench M. Zuidhof C. Bench M. Zuidhof C. Bench M. Zuidhof

5,000

M. Zuidhof

15,000

M. Zuidhof

247,159

M. Zuidhof

30,000 35,176

2013 20112014 20112014 20112013 20112013 20122013 20112013

Learning/Teaching projects Granting Body

Abbreviated Title

ALMA 2012L094D Aviagen, Inc.

A workshop for Canadian Hatchery Professionals Managing Breeders in the 21st Century A workshop for Canadian Hatchery Professionals Managing Breeders in the 21st Century

ALMA 2012L094D Aviagen, Inc.

Page | 34

The PRC Annual Report 2013

60,000 419,257 17,500 10,000 10,000 247,159

$46,000 Amount received in current year $8,000

Applicant(s)

Planned Duration

Total value of grant

V. Carney

2012

$8,000

$15,000

V. Carney

2012

$15,000

$8,000

V. Carney

2012

$8,000

$15,000

V. Carney

2012

$15,000

PRC Financials 2012-2013 Income 2012-2013 Income 2012 - 2013 Industry Alberta Turkey Producers Alberta Hatching Egg Producers Alberta Chicken Producers Egg Farmers of Alberta Egg Farmers of Canada Burnbrae Farms Poultry Health Services Sub-total Opening balance* TOTAL INDUSTRY

Actuals as of March 31, 2013 Cash In-Kind 27,225.00 30,973.00 87,656.00 32,000.00 15,000.00 8,500.00 35,000.00 201,354.00 35,000.00 156,407.85 357,761.85 35,000.00

Total 27,225.00 30,973.00 87,656.00 32,000.00 15,000.00 8,500.00 35,000.00 236,354.00 392,761.85

*"Opening balance" represents funds remaining from original PRC agreement; 2012/2013 amounts represent contributions made for year 1 of the new 2012-2017 agreement

AARD Opening balance TOTAL AARD ALMA* AI- BIO Sub-total Opening balance TOTAL ALMA/AI-BIO

43,000.00 0.00 43,000.00

337,600.00

380,600.00

337,600.00

380,600.00

900,000.00 100,000.00 1,000,000.00 0.00 1,000,000.00

900,000.00 100,000.00 1,000,000.00 1,000,000.00

*ALMA provided most of the three years of funding in year 1

UofA UofA/AFNS Poultry Unit (Internal revenue) Poultry Unit (External revenue) Sub-total Opening balance Total UofA

Total Income

188,957.82 29,537.34 169,784.80 388,279.96 -17,358.00 370,921.96 Cash 1,771,683.81

1,552,480.00

1,552,480.00

1,741,437.82 29,537.34 169,784.80 1,940,759.96 -17,358.00 1,923,401.96

In-Kind 1,925,080.00

Total 3,696,763.81

1,552,480.00

The PRC Annual Report 2012

Page | 35

PRC Funding - Industry sources received in 2012-2013 ($201,354) Egg Farmers of Burnbrae Farms Canada 4% 7% Egg Farmers of Alberta 16%

Alberta Turkey Producers 14% Alberta Hatching Egg Producers 15%

PRC Funding - All sources received in 2012-2013 ($1,771,684)

Poultry Unit, PHS, in kind external 1% Industry 5% 6%

ARD 1%

Poultry Unit, internal 1%

AI-Bio 3%

U of A/AFNS 5%

Page | 36

The PRC Annual Report 2013

Expenses 2012-2013 Expenses 2012 - 2013 Salaries and benefits Supplies Travel Equipment Total Expenses

Actuals as of March 31, 2013 Cash In-Kind 936,260.39 132,813.45 5,253.64 16,462.03 1,090,789.51 1,925,080.00

Total 936,260.39 132,813.45 5,253.64 16,462.03 3,015,869.51

Revenue/Expense Summary 2012 - 2013 Actuals as of March 31, 2013 Cash In-Kind Total Total Income (incl. carryover) 1,771,683.81 1,925,080.00 3,696,763.81 Total Expenses 1,090,789.51 1,925,080.00 3,015,869.51 Total carryover to 2013/14 680,894.30 0.00 680,894.30

The PRC Annual Report 2013

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Revenue Budget 2013-2014 Income 2013 -2014 (Budget) Industry Alberta Turkey Producers Alberta Hatching Egg Producers Alberta Chicken Producers Egg Farmers of Alberta Egg Farmers of Canada Burnbrae Farms Lilydale* Maple Leaf* Poultry Health Services Sub-total Opening balance TOTAL INDUSTRY

Cash 28,042.00 31,902.00 92,016.00 33,600.00 15,000.00 8,500.00 28,787.00 28,787.00 266,634.00 85,117.44 351,751.44

In-Kind

35,000.00 35,000.00 35,000.00

Total 28,042.00 31,902.00 92,016.00 33,600.00 15,000.00 8,500.00 28,787.00 28,787.00 35,000.00 301,634.00 85,117.44 386,751.44

*Represents year 1 and 2 payments

AARD Opening balance TOTAL AARD

43,000.00 10,000.00 53,000.00

337,600.00 337,600.00

380,600.00 10,000.00 390,600.00

ALMA AI- BIO Sub-total Opening balance TOTAL ALMA/AI-BIO

31,466.00 100,000.00

31,466.00 100,000.00

570,609.94 702,075.94

570,609.94 702,075.94

UofA UofA/AFNS Poultry Unit (Internal revenue) Poultry Unit (External revenue) Sub-total Opening balance Total UofA

200,800.00 30,000.00 160,000.00 390,800.00 15,166.92 405,966.92

Total Income

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Cash 1,512,794.30

The PRC Annual Report 2013

1,552,480.00

1,552,480.00

1,753,280.00 30,000.00 160,000.00 1,943,280.00 15,166.92 1,958,446.92

In-Kind 1,925,080.00

Total 3,437,874.30

1,552,480.00

Expense Budget 2013-2014 Expenses 2013 - 2014 (Budget) Salaries and benefits Supplies Travel Equipment Total Expenses

Cash 785,512.00 187,000.00 18,000.00 25,000.00 1,015,512.00

In-Kind

1,925,080.00

Total 785,512.00 187,000.00 18,000.00 25,000.00 2,940,592.00

Revenue/Expense Summary 2013-14 Revenue/Expense Summary 2013 -2014 (Budget) Cash In-Kind Total Total Income (incl. carryover) 1,512,794.30 1,925,080.00 3,437,874.30 Total Expenses 1,015,512.00 1,925,080.00 2,940,592.00 Total carryover to 2014/15 497,282.30 0.00 497,282.30

The PRC Annual Report 2013

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Poultry Unit Financial Report 2012-2013

Unit Report Unit Financial Report 2012 -2013 Income 2012 - 2013 UofA/AFNS Poultry Unit (Internal revenue) Poultry Unit (External revenue) Sub-total Opening balance Total Income

Expenses 2012 - 2013 Salaries and benefits Supplies Equipment Total Expenses*

Actuals as of March 31, 2013 Total 188,957.82 29,537.34 169,784.80 388,279.96 -17,358.00 370,921.96

Actuals as of March 31, 2013 Total 280,684.66 58,608.35 16,462.03 355,755.04

*Additional unit costs of $100,000 were moved to in Industry, ARD, and ALMA funding

Income/Expense Summary 2012 - 2013 Actuals as of March 31, 2013 Total Income 370,921.96 Total Expenses 355,755.04 Carryover/surplus to 2013/14 15,166.92

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The PRC Annual Report 2013

Poultry Unit Budget 2013-2014

Unit Budget 2013 - 2014 Income 2013 - 2014 (budget) Total 200,800.00 30,000.00 160,000.00 390,800.00 15,166.92 405,966.92

UofA/AFNS Poultry Unit (Internal revenue) Poultry Unit (External revenue) Sub-total Opening balance Total Income

Expenses 2013 - 2014 (budget) Total 300,512.00 70,000.00 25,000.00 395,512.00

Salaries and benefits Supplies Equipment Total Expenses* *Additional unit costs of $100,000 have been budgeted in Industry and ARD funding

Income/Expense Summary 2013 - 2014 (budget) Total Income Total Expenses Carryover (surplus) to 2014/15

405,966.92 395,512.00 10,454.92

The PRC Annual Report 2013

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Facility Usage Research Facilities Utilization Rate (%) Facility

Overall

Brooder house (48 floor pens) Breeder hen cages (288 individual cages) Breeder male cages (60 individual cages) Nutrition house (32 pens) Specht pullet cages (64 group cages) Environmental chambers Test house - Floor pens (rare breeds) Test House - Conventional cages Test House - Colony cages Broiler Processing Plant (3 days per use) Hatchery Setter use AVN Hatcher use AVN Setter use BIG J Hatcher use BIG J

58

Broiler & Turkey Trials 1

Breeder Trials 20

91

73

73

73

52

52

19

19

Layer Trials

Unit Operations 37

18

100

100

100 100

100 100

3

3

19 18 5 5

19 18 4 4

1 1

Non-Research Facilities Facility Lilydale Room

Alberta Turkey Producers Computer Lab

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Description Combined producer meetings Processors PRC alumni, exec group & educational institutions U of A, safety, animal care, animal handling, HACCP Industry related workshops (swine, dairy, AAF, etc.)

Student presentations & community learning Heavy use by graduate students, undergraduate students, technicians and researchers

The PRC Annual Report 2013

Utilization 1 days 73 days 7 days 4 days 25 days 25 person hours /d

Evidence of Productivity Articles published in refereed journals (n=26) 1. Acero, A., A. Ullah, M. Offendenden, S. Jung, and J. Wu. 2012. Effect of high-pressure treatment on structure of ovotransferrin. Food Chemistry. 135:2245–2252.

2. Bandara, N., H. Zeng, and J. Wu. 2012. Marine mussel adhesion: Biochemistry, mechanisms and biomimetics. Journal of Adhesion Science and Technology. 24 pp. (Available online DOI:10.1080/01694243.2012.697703).

3. Bandara N, Chen L, Wu J. (2013) Adhesive properties of triticale distillers grain. International Journal of Adhesion and Adhesives. 44:122-129 4. Franco Ulmer, A., G. Cherian, N. Quezada, G. M. Fasenko, and L. M. McMullen. 2012. Hatching egg yolk and newly hatched chick yolk sac total IgY content at three broiler breeder flock ages. Poult. Sci. 91:758-764.

5. Hrynets Y., M. Ndagijimana, and M. Betti. 2013. Non-enzymatic glycation of natural actomyosin (NAM) with glucosamine in a liquid system at moderate temperatures. Food Chemistry 139:1062– 1072.

6. Huang, W-Y., S. Shen, C. Nimalaratne, S. Li., K. Majumder, and J. Wu. 2012. Effects of addition of egg protein ovotransferrin-derived peptides on oxygen radical absorbance capacity of different teas.

Food Chemistry 135:1600-1607.

7. Intarasirisawat, R., S. Benjakul, W. Visessanguan, and J. Wu. 2012. Antioxidative and functional properties of protein hydrolysate from defatted skipjack (Katsuwonous pelamis) roe. Food Chemistry 135:3039-3048.

8. Korver, D. R. 2012. Implications of changing immune function through nutrition in poultry. Anim.

Feed Sci. Technol. 173:54–64.

9. Liu Y, M. Betti, and M. Gaenzle. 2012. High pressure inactivation of Escherichia coli, Campylobacter jejuni, and spoilage microbiota on poultry meat. Journal of Food Protection 75:497-503.

10. Nain, S., R. A. Renema, D. R. Korver, and M. J. Zuidhof. 2012. Characterization of the n-3 polyunsaturated fatty acid enrichment in laying hens fed an extruded flax enrichment source. Poult.

Sci. 91:1720–1732.

11. Nain, S., R. A. Renema, M. J. Zuidhof, and D. R. Korver. 2012. Effect of metabolic efficiency and intestinal morphology on variability in n-3 polyunsaturated fatty acid enrichment of eggs. Poult. Sci. 2012. 91:888-898.

12. Nakano T., L. Ozimek, and M. Betti. 2012. Boning broiler chicken legs and wings by dislocation of articular cartilage followed by stripping periosteum. Poultry Science 91:2938-2941.

13. Nakano T., L. Ozimek, and M. Betti. 2012. Immunological detection of keratin sulfate in meat products with and without mechanically separated chicken meat. Meat Science 92:867-9.

14. Nakano T., Z. Pietrasik, L. Ozimek, and M. Betti. 2012. Extraction, isolation and analysis of chondroitin sulfate from broiler chicken biomass. Process Biochemistry 47:1909-1918.

15. Nimalaratne, C., Lopes-Lutz, D., Schieber, A., and J. Wu. 2012. Effect of domestic cooking methods on egg yolk xanthophylls. Journal of Agricultural and Food Chemistry 60:12547-52.

16. Omana D. A., Z. Pietrasik and M. Betti. 2012. Evaluation of poultry protein isolate as a food ingredient: Physicochemical characteristics of low fat turkey bologna. Poultry Science 91:3223-9.

17. Omana D. A., Z. Pietrasik and M. Betti. 2012. Use of poultry protein isolate as a food ingredient: sensory and color characteristics of low fat poultry bologna. Journal of Food Science 77:S274-S280.

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18. Schneider B. L., R. A. Renema, M. Betti, V. L. Carney and M. J. Zuidhof. 2012. Effect of holding temperature, shackling, sex and age on broiler breast meat quality. Poultry Science 91:468-77.

19. Silversides, F. G., R. Singh, K. M. Cheng, and D. R. Korver. 2012. Comparison of bones of 4 strains of laying hens kept in conventional cages and floor pens. Poultry Science 91:1–7.

20. Srichamroen A., T. Nakano, Z. Pietrasik, L. Ozimek, and M. Betti. 2012. Chondroitin sulfate extraction from broiler chicken cartilage by tissue autolysis. LWT Food Science and Technology 50:607-612.

21. Ullah, A., and J. Wu. 2013. Feather fiber-based thermoplastics: Effects of different plasticizers on material properties. Macromolecular Materials and Engineering 298:153-162.

22. Vail, K. M., L. M. McMullen and T. H. Jones. 2012. Growth and filamentation of cold-adapted log phase Listeria monocytogenes exposed to salt, acid, or alkali stress at 3°C. J. Food Protect. 75:21422150.

23. Wang H., J. Wu and M. Betti. 2013. Chemical, rheological and surface morphologic characterization of spent hen proteins extracted by pH-shift processing with or without the presence of cryoprotectants. Food Chemistry 139:710–719.

24. Wang, C., and J. Wu. 2012. Preparation and characterization of adhesives from spent hen proteins.

International Journal of Adhesion and Adhesives 36:8-14.

25. Wang, J., and J. Wu. 2012. Effect of operating conditions on ovomucin extraction. Process Biochemistry. 47:94–98.

26. Wu, J., and A. Acero. 2012. Ovotransferrin: structure, function and preparation. Food Research International 46:480-487. (Invited paper) 27. Yegani, M. and D. R. Korver. 2012. Review: Prediction of variation in energetic value of wheat for poultry. Can. J. Anim. Sci. 92:261-273.

Proceedings 1. DesLauriers, A. G. C., N. A. Mudryk, C. L. J. Phimester, M. J. Zuidhof, D. R. Korver, A. Pishnamazi, P. O. Elaho, and R. A. Renema. 2012. Comparison of in-barn hatching to conventional hatching techniques on broiler growth traits. Proceedings of the XXIV World’s Poultry Congress. Salvador, Bahia, Brazil.

August 2012. 4 pp.

2. Honarbakhsh, S., A. Pishnamazi, E. Mba, M. Zuidhof and R. Renema. 2012. Will the most energetically efficient broiler breeder pullets resulting from rearing diets varying in protein and energy have higher subsequent reproductive performance? Proceedings of the XXIV World’s Poultry Congress. Salvador, Bahia, Brazil. August 2012. 4 pp.

3. Moraes T. G. V., A. Pishnamazi, I. I. Wenger, E. T. Mba, R. A. Renema, M. J. Zuidhof. 2012. Effect of maternal diet composition on broiler body weight and carcass yield. Proceedings of the XXIV World’s Poultry Congress. Salvador, Bahia, Brazil. August 2012. 4 pp.

4. Pietrasik, Z., D. Pierce, J. Zhang, and L. M. McMullen. 2012. Effect of post-packaging steam pasteurization on quality and consumer acceptance of fully cooked vacuum-packaged sliced turkey breast. Proceedings of the 58th International Congress of Meat Science and Technology, Montreal, Canada, August 12-17. SAFMICROP-19, 1-4.

5. Pishnamazi, A., M. J. Zuidhof, R. A. Renema, D. C. Paul and I. I. Wenger. 2012. Comparison of energy efficiency of free run or caged broiler breeder hens can aid interpretation of caged bird nutritional studies. Proceedings of the XXIV World’s Poultry Congress. Salvador, Bahia, Brazil. August 2012. 4 pp.

6. Renema, R. A., and M. J. Zuidhof. 2012. Devil in the details: Growing females (3 to 23 wk).

Proceedings of the ‘Setting the Stage for Success: Managing Breeders in the 21st Century’ conference. Oct. 2-4, Airdrie, AB. 3 pp.

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The PRC Annual Report 2013

7. Renema, R. A., and M. J. Zuidhof. 2012. Taking Care of Business: A Chick or Two Can Mean a Lot to You. Proceedings of the ‘Setting the Stage for Success: Managing Breeders in the 21st Century’ conference. Oct. 2-4, Airdrie, AB. 2 pp.

8. Renema, R. A., M. J. Zuidhof, and F. E. Robinson. 2012. Management of a changing broiler breeder: Do we need to change, too? Proceedings of the Poultry Service Industry Workshop, Banff, AB. September 25-27, 2012, 8 pp.

9. Robinson, F. E., D. C. Penrice, and M. J. Zuidhof. 2012. Beyond Conventional Teaching in Animal Science: Experiences in “Free Range” Learning. Proceedings of the National Conference on Teaching and Learning in the Animal Sciences. June 19-22, 2012. Madison, WI. 16 pp.

10. Ton, K. P., J. L. Saunders-Blades, D. R. Korver, and M. J. Zuidhof. 2012. Effect of early nutrition, 25OH-D3, and antibiotic on broiler innate immunity and ileal morphology. Proceedings of the XXIV World’s Poultry Congress. Salvador, Bahia, Brazil. August 2012. 4 pp.

11. Urrutia, S., Y. Chen; A. Pishnamazi; M. Zuidhof; L. Guan; and R. Renema. 2012. Impact of genetic selection and dietary antibiotics on the ability of the gut microflora to tolerate a feed interruption.

Proceedings of the XXIV World’s Poultry Congress. Salvador, Bahia, Brazil. August 2012. 4 pp.

12. Wenger, I. I., P. O. Elaho, M. J. Zuidhof, D. R. Korver, and A. Pishnamazi. 2012. The economics of early nutrition and antibiotics on broiler production. Proceedings of the XXIV World’s Poultry Congress. Salvador, Bahia, Brazil. August 2012. 4 pp.

13. Zuidhof, M. J., R. A. Renema, and A. Pishnamazi. 2012. Current advances in broiler breeder modeling research: A North American perspective. AMEVEA, Bogotá, Colombia, 18-19 April, 2012, 10 pp.

Presentations 1. Beltranena, E. and M. Oryschak. 2012. Fractionation of canola meal to increase its energy value for broilers. Canola Council Meeting at 33rd Western Nutrition Conference, Winnipeg, MB, Sep 23.

2. Beltranena, E., and M. Oryschak. 2012. Feeding high dietary inclusions of expeller-pressed or solvent-extracted, lower-fibre yellow seeded vs. conventional dark-seeded canola meal to layers on egg production and quality. Canola Council Meeting at 33rd Western Nutrition Conference, Winnipeg, MB, Sep 23.

3. Betti M. 2012. Possibilities for an in vitro meat production system: a food scientist’s perspective.

Institute of Food Technologist Annual Meeting & Food Expo, June 25–29, 2012. Oral – Invited 4. Betti. M. 2012. A novel approach to produce glycopeptides from food proteins. 8th International Symposium on Biocatalysis and Agricultural Biotechnology, Sonoma Wine Country (California) October 28-31, 2012. Oral - Invited.

5. Carney, V. L., B. L. Schneider and F. E. Robinson. 2012. Evaluating male quality. Poultry Sci. 91(Supp1):60.

6. Carney, V. L., B. L. Schneider, R. A. Renema, F. E. Robinson. 2012. External indicators of reproductive condition in commercial broiler breeder flocks. World’s Poultry Science Journal 68(E-Supp).

The PRC Annual Report 2013

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7. The following presentations were given during a speaking tour of Southeast Asia July 16-Augst 1, 2012. I visited with 11 large poultry nutrition companies, and gave two egg producer seminars. The specific presentations varied from company depending on interests and amount of time, and included 9 different seminar presentations: a. Korver, D. R., S. Singla, D. Yu, W. Wismer and M. Betti. “Black Bone” Discolouration in Poultry Meat. b. Korver, D. R. Bone Density in Layers and Broiler Breeders. c. Korver, D. R. & R. A. Renema. Broiler Breeder Amino Acid Requirements. d. Korver, D. R., C. A. Torres, M. L. Johnson, J. Saunders-Blades & J. Hamidu. Factors Affecting Broiler Chick Quality. e. Korver, D. R. & R. A. Renema. Nutrient Enrichment of Table Eggs. f. Korver, D. R. Eggshell Quality in Laying Hens. g. Korver, D. R. Maternal Nutrient Transfer to the Chick h. Korver, D. R. Interaction of Nutrition & Immune Function in Broiler Chickens i. Korver, D. R. Unintended Consequences: The Effect of Genetic Selection on Immune Function in Broilers.

8. Hamidu J. A.,* C. A. Torres, M. L. Johnson, and D. R. Korver. 2012. Effect of hen age and late incubation temperature on embryo development. 2. Chick quality. Poult. Sci. 91(E-Suppl. 1):13.

9. Hamidu, J. A. *, C. A. Torres, M. L. Johnson, and D. R. Korver. 2012. Effect of hen age and late incubation temperature on embryo development. 1. Embryo metabolism. Poult. Sci. 91(E-Suppl.

1):13.

10. Johnson, M. L. *, J. L. Saunders-Blades, and D. R. Korver. 2012. The effect of maternal vitamin E supplementation, hen age and chick age on chick early innate immune function. Poult. Sci. 91(ESuppl. 1):20.

11. Johnson, M. L. *, J. A. Hamidu, C. A. Torres, and D. R. Korver. 2012. Effect of hen age and late incubation temperature on embryo development. 3. Early innate immune function. Poult. Sci. 91(ESuppl. 1):14 Torres, C. A. *, J. A. Hamidu, M. L. Johnson, and D. R. Korver. 2012. Effect of hen age and late incubation temperature on embryo development. 4. Bone development. Poult. Sci. 91(E-Suppl.

1):14.

12. Korver, D. R. 2012. Efectos de 25-OH D3 en: Desarrollo intestinal, producción de pechuga, inmunidad y desarrollo óseo en aves (Effect of 25-OH D3 on intestinal development, production of breast, immunity and bone development in birds). DSM Technical Seminar on Management of Broiler Breeders. August 29, 2012. Bogota, Colombia.

13. Korver, D. R. 2012. Hatching Egg Shell Quality – Measuring and How to Keep it. Aviagen Latin America Ross Association. November 28, 2012.

14. Korver, D. R. 2012. Hy-D®: research and new applications. DSM technical seminar. Panamá City, Republica de Panamá May 24, 2012.

15. Korver, D. R. 2012. Implications of changing immune function through nutrition. Pre-conference symposium: Nutrition and Immunology. Arkansas Nutrition Conference. September 4, 2012. Rogers, AR. 16. Korver, D. R. 2012. Interacción de la Nutrición y la Función Inmune en Pollo de Engorde (Interaction of Nutrition and Immune Function in Broiler Chickens). XXXI Seminario Internacional AMEVEA (Asociacion de Medicos Veterinarios Especialistas en Avicultura). Bogota, Colombia. April 19, 2012.

17. Korver, D. R. 2012. Manejo densidad osea gallinas ponedoras (Bone Density in Layers and Broiler Breeders) XXII Congreso Centroamericano y del Caribe de Avicultura. Panamá City, Republica de Panamá May 24, 2012.

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The PRC Annual Report 2013

18. Korver, D. R. 2012. Manejo y nutrición para controlar la calidad de la cáscara del huevo (Eggshell Quality in Laying Hens). XV International Poultry Seminar, Asociacion de Medicos Veterinarios Especialistas en Avicultura del Ecuador. Invited technical seminar. April 18, 2012.

19. Korver, D. R. 2012. Nutrition and Immunity: Opportunities and Unintended Consequences.

Kitchener, ON. May 10, 2012.

20. Korver, D. R. 2012. Optimizando el metabolismo óseo para la salud y la productividad de ponedoras comerciales y reproductoras pesadas (Bone Density in Layers and Breeders). XV International Poultry Seminar, Asociacion de Medicos Veterinarios Especialistas en Avicultura del Ecuador. Invited technical seminar. Quito, Ecuador. April 18, 2012.

21. Korver, D. R. 2012. Poultry Nutrition Research at the University of Alberta. Diamiond V Mills, Cedar Rapids, IA June 21, 2012.

22. Korver, D. R. *, S. Singla, D. Yu, W. Wismer and M. Betti 2012. Causes and Factors Affecting Black Bone. Invited Presentation, Kasetsart University, Kampaengsaen Campus, Thailand. July 19, 2012.

23. M. Betti 2012. Innovative functional properties from poultry processing by-products. Midwest Poultry. Midwest Poultry Federation Convention, St Paul, MN, U. S. 24. M. Betti 2012. Innovative functional properties from poultry processing by-products. Midwest Poultry. Midwest Poultry Federation Convention, St Paul, MN, U. S. Oral - Invited.

25. Majumder, K., Panahi, S., Kaufman S. Wu, J. (2012). Egg reduces high blood pressure in spontaneously hypertensive rats. Poster presentation at the 16th World Congress of Food Science and Technology, Foz do Iguacu, Parana State, Brazil, August 5-9.

26. Majumder, K., Panahi, S., Kaufman S. Wu, J. (2012). Fried whole egg hydrolysate reduces high blood pressure in spontaneously hypertensive rats. Poster presentation at the 2012 IFT Annual Meeting & Food Expo June 25 - 28, 2012, Las Vegas, Nevada, USA. 27. Navidghasemizad, S., Temelli, F. and Wu, J. (2012). Enzymatic Hydrolysis Impact on Value Added Phospholipids Extractability from Leftover Egg Yolk. Poster presentation at the10th International Symposium on Supercritical Fluids, May 13 – 16, San Francisco, CA, USA. 28. Navidghasemizad, S., Temelli, F. and Wu, J. (2012). Polysaccharide-aided Extraction of LDL from Egg Yolk. Poster presentation at the 2012 IFT Annual Meeting & Food Expo June 25 - 28, Las Vegas, Nevada, USA. 29. Nimalaratne, C., Schieber, A. & Wu, J. (2012). Antioxidative compounds in egg yolk. at 2012 International Society of Nutraceuticals & Functional Foods. December 2-6, Kona, Hawaii, USA. 30. Offengenden, M. and Wu, J. (2012). Weak gel properties of ovomucin gel from chicken egg white.

Poster presentation at the 2012 Banff Egg Forum, March 28-30, Banff, Edmonton, Canada.

31. Offengenden, M. and Wu, J. Ovomucin from hen egg white shows weak gel properties. Poster presentation at the 16th World Congress of Food Science and Technology, Foz do Iguacu, Parana State, Brazil, 2012, August 5-9.

32. Omana, D. A., Y. Hrynets, Z. Pietrasik and M. Betti. 2012. Evaluation of poultry protein isolate as a brine ingredient during marination of chicken breast meat. Institute of Food Technologist Annual Meeting & Food Expo, June 25–29, 2012.

33. Omana, D. A., Y. Hrynets, Z. Pietrasik and M. Betti. 2012. Evaluation of poultry protein isolate as a brine ingredient during marination of chicken breast meat. Institute of Food Technologist Annual Meeting & Food Expo, June 25–29, 2012. Poster.

34. Oryschak, M. 2013. Could coproducts give corn-soybean a run for your money? Proceedings of the 2013 Atlantic Poultry Conference in Wolfville, NS, Feb 14.

35. Oryschak, M. *, D. Korver, and E. Beltranena. 2012. Nutrient digestibility in Canadian-grown pulse crops compared to soybean meal for growing broilers at 15- and 29-d of age. Poult. Sci. 91(Suppl.

1):136.

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36. Oryschak, M. *, D. Korver, and E. Beltranena. 2012. Nutrient digestibility in air-classified pulse protein concentrates and wet fractionated soy protein concentrate for 15-d-old broiler chicks. Poult.

Sci. 91(Suppl. 1):43.

37. Oryschak, M., and E. Beltranena. 2012. Nutrient and energy digestibility in air-classified faba bean and field pea protein and starch concentrates in 21-d old broilers. Abstr. P410, Poultry Sci. 91(Suppl.

1):137.

38. Oryschak, M., B. Slominski, and E. Beltranena. 2012. Effect of increasing dietary inclusion of solventextracted B. juncea vs. B. napus canola meal on broiler growth performance, carcass traits and yield of carcass components. Abstr. 251, Poultry Sci 91(Suppl. 1):86.

39. Oryschak, M., C. Annett, and E. Beltranena. 2012. Screw-pressed Camelina sativa meal as feedstuffs for broilers: Effects of graded dietary inclusion on organ weight and post-mortem signs of toxicity.

Abstr. P404, Poultry Sci. 91(Suppl. 1):137.

40. Oryschak, M., D. Korver, and E. Beltranena. 2012. Nutrient digestibility in Canadian-grown pulse crops compared with soybean meal for growing broilers at 15- and 29-d of age. Abstr. 121, Poultry Sci. 91(Suppl. 1):43.

41. Possibilities for an in vitro meat production system: a food scientist’s perspective. Institute of Food Technologist Annual Meeting & Food Expo, June 25–29, 2012.

42. Renema, R. A., M. J. Zuidhof and D. R. Korver. Broiler Breeder Management Workshop. XXXI Seminario Internacional AMEVEA (Asociacion de Medicos Veterinarios Especialistas en Avicultura).

Bogota, Colombia. April 20, 2012.

43. Robinson*, F. E., D. C. Penrice, and M. J. Zuidhof. Ag Awareness. Invited team presentation to Rural Physician’s conference. Edmonton, AB. November 24, 2012. Audience of 50.

44. Robinson*, F. E., D. C. Penrice, and M. J. Zuidhof. FarmAssist. Invited team presentation to Faculty of Pharmacy rural internship program orientation. Edmonton, AB. October 25, 2012. Audience of 80.

45. Schneider, B. L., V. L. Carney and F. E. Robinson. 2012. Standardization of on-farm fertility tests.

Poultry Sci. 91(Supp1):60.

46. Schneider, B. L., V. L. Carney, R. A. Renema, F. E. Robinson. 2012. Predictors of end-of-lay reproductive status in commercial broiler breeders. World’s Poultry Science Journal 68(E-Supp).

47. Torres, C. A. *, D. R. Korver and J. A. Hamidu. 2012. Effect of incubator temperature and parent flock age on chick bone characteristics at hatch. World’s Poultry Congress. Aug. 5-9, 2012 Salvador, Brazil.

48. Wang H., J. Wu and M. Betti. (2012). Chemical and Rheological Properties of Proteins Recovered from Spent Hens Mince by pH-shift Technology With and Without Cryoprotectants Addition. Poster presentation at the 2012 IFT Annual Meeting & Food Expo June 25 - 28, Las Vegas, Nevada, USA. 49. Wang H., J. Wu and M. Betti. 2012. Chemical and rheological properties of proteins recovered from spen hen mince by pH shift technology with and without cryoprotectants. Institute of Food Technologist Annual Meeting & Food Expo, June 25–29, 2012.

50. Wang H., J. Wu and M. Betti. 2012. Chemical and rheological properties of proteins recovered from spen hen mince by pH shift technology with and without cryoprotectants. Institute of Food Technologist Annual Meeting & Food Expo, June 25–29, 2012. Poster 51. Wang, C. and Wu, J. (2012). Adhesive from a cruciferin-poly(glycidyl methacrylate) conjugate: Characteristics and optimization. Oral presentation at the Forestry Product Society’s 66th International Convention. June 3-5Washington, D. C., USA. 52. Wang, C. and Wu, J. (2012). Utilization of spent-hen protein for wood adhesive. Oral presentation at the 2103rd AOCS annual meeting. April 30 - May 2, Long Beach, CA, USA. 53. Wenger I. I., P. O. Elaho, M. J. Zuidhof, D. R. Korver. 2012. The Economics of Early Nutrition and Antibiotics on Broiler Production. Proceedings of the XXIV World’s Poultry Congress. Salvador, Bahia, Brazil. August 2012. 4 pp.

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54. Wenger, I. I. 2013. Developing strategies for the Alberta Turkey to improve turkey welfare and quality. Invited presentation to the Alberta Turkey Producers Annual General Meeting. Red Deer, AB. February 26, 2013 (52 people).

55. Wu, J. (2012). Food protein derived antihypertensive peptides: alternative to Captopril? Shuryo Nakai Memorial Symposium at 2012 International Society of Nutraceuticals & Functional Foods.

December 2-6, Kona, Hawaii, USA. (Invited speaker) 56. Wu, J. Bioactive Peptides from Egg Proteins: Opportunities & Perspectives. Oral presentation at the 2012 International Symposium on Egg Industry. June 18-19, Beijing, China. (Invited speaker) 57. Wu, J. Ullah, A. Bioplastic from Feather Quill. (2012). Oral presentation at the 2103rd AOCS annual meeting. April 30 - May 2, Long Beach, CA, USA. 58. Wu, J., Li, S., Fentabil, M., Gänzle, M. G., Offengenden, M. (2012). Effect of Lactobacilli fermentation on IgE binding ability of egg white proteins. Oral presentation at the Canadian Institute of Food Science and Technology (CISFT)’s 50th annual meeting. May 27-29, Niagara Falls, Ontario, Canada.

59. Zuidhof, M. J. 2012. Broiler breeder research update. Hybrid Turkeys, Kitchener, ON, June 6, 2012 (14 people).

60. Zuidhof, M. J. 2012. Ideal broiler breeder growth and nutrient supply (energy / protein) profiles for quantity and quality of chicks. DSM Seminar, Bogotá, Colombia, 29 August, 2012 (250 people).

61. Zuidhof, M. J. 2012. Nutritional mitigation strategies for antibiotic free broiler production.

Nutritional Mitigation Strategies for Antibiotic Free Broiler Production. Status update meeting – Life cycle assessment of arsenic from poultry feed to a food grain crop. Edmonton, AB. 29 November, 2012 (15 people).

62. Zuidhof, M. J. 2012. Precision broiler breeder feeding: Applying 2 decades of learning. Agriculture and Food Council Annual General Meeting, Edmonton, AB, June 26, 2012 (150 people).

63. Zuidhof, M. J. 2012. Research Report. PRC Advisory Board meeting, Edmonton, AB, May 23, 2012 (24 people).

64. Zuidhof, M. J., A. Pishnamazi, I. I. Wenger, and R. A. Renema. 2012. Environmental temperature and dietary protein affect energy requirements, yield, and meat quality of broilers. Poultry Sci. 92(Suppl.

1):165 (85 people).

65. Zuidhof, M. J., D. R. Korver, C. Lozano, and R. A. Renema. 2012. Broiler Breeder Workshop. AMEVEA, Bogotá, Colombia, 19 April, 2012 (40 people).

66. Zuidhof, MJ, Korver, DR, Carney, VL. 2012. PRC Research Update and Dialogue. Alberta Chicken Producers Regional Meetings, Edmonton, Red Deer and Lethbridge a. Lethbridge, October 29, 2012 (Presenter: Zuidhof) b. Red Deer, October 30, 2012 (Presenter: Carney) c. Edmonton, October 31, 2012 (Presenter: Carney)

Research Reports 1. Annual report “Development of a comprehensive platform for the value-added utilization of poultry products”. CFI Annual report submitted on line June 30, 2012.

2. Final report “Banff Egg Forum 2012”, submitted on October 30, 2012 to NSERC, project leader Dr. Erasmus Okine. 4 pages.

3. Final report “Banff Egg Forum 2012”, submitted on October 30, 2012 to Alberta Livestock and Meat Agency Ltd., project leader Dr. Erasmus Okine. 4 pages.

4. Final report “Development of Bioplastic from feathers”, submitted on February 15, 2012 to Biorefining Conversion Network/Alberta Advanced Education and Technology, 9 pages.

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5. Final report for “ Utilization of protein-containing agriculture waste and by-products for adhesive development (#2009F082R)”, submitted on November 30, 2012 to Agriculture Funding Consortium, final report 30 pages, tables and figures18 pages 6. Final report for “ Utilization of protein-containing agriculture waste and by-products for adhesive development (#2009F082R)”, submitted on October 31, 2012 to Canadian Poultry Research, final report 11 pages, 1 page executive summary.

7. Final report for “Exploring the health promoting components in laying hen eggs for the development of value-added egg components (#AB0545)”, submitted on April 15, 2012 to Alberta Agriculture and Food Council, final report 15 pages.

8. Final report for “Integrated Utilization of Leftover Egg Yolk Pellet After Antibody Extraction (#2008F020R)”, submitted on August 30, 2012 to Agriculture Funding Consortium, final report 28 pages, tables and figures 24 pages and appendices 154 pages.

9. Guan, L. L., R. A. Renema, S. Urrutia, M. J. Zuidhof, and E. Okine. 2012. Project title: Impact of genetic selection and dietary antibiotics on the ability of the gut microflora to tolerate a feed interruption. Final report to Alberta Livestock and Meat Agency: Project 2010F157R. December, 2012. 27 pp.

10. Gutfreund, K. J. L. Saunders-Blades and D. R. Korver. 2012. Immunotargeting with CD154 (CD40L) to improve avian influenza vaccine responses in chicken. Final project report to Agriculture & Food Council. 16 pages.

11. Interim report “Antihypertensive activity of laying hen eggs”, submitted to Agriculture and Food Council on June 30, 2012, 3 pages.

12. Interim report “Exploring health promoting components in laying hen eggs for the development of value-added egg components (#AB1123)”, submitted to Agriculture and Food Council on June 30, 2012, 3 pages.

13. Interim report “Functional egg bioactive peptides: scale-up processing and in vivo efficacy (# 2011F027R)”, submitted on August 31, 2012, 11 pages.

14. Interim report “Learning from grandma: developing valuable functional food ingredients from spent hens (#2012R023R)”, submitted on August 31, 2012, 11 pages.

15. Johnson, M. L. & D. R. Korver. 2012. Nutrient enrichment of broiler breeder diets to improve broiler chick quality and immune function. Final project report to Agriculture & Food Council. 95 pages.

16. Johnson, M. L. & D. R. Korver. 2012. The effect of maternal canthaxanthin and hen age supplementation on indices of chick early innate immune function. Final project report to DSM Nutritional Products. 45 pages.

17. Johnson, M. L. & D. R. Korver. 2012. The effect of maternal vitamin e and hen age supplementation on indices of chick early innate immune function. Final project report to ADM Alliance Nutrition. 35 pages.

18. Johnson, M. L. & D. R. Korver. 2012. The effect of maternally supplemented l-carnitine and hen age on indices of chick early innate immune function. Final project report to ADM Alliance Nutrition. 34 pages.

19. Oryschak, M., and E. Beltranena. 2012. Comparison of ileal endogenous amino acid measurements in growing broilers fed an N-free diet or a diet containing a source of highly digestible protein. Abstr.

P337, Poultry Sci 91(Suppl. 1):115.

20. Renema, R. A., T. Moraes, E. Mba, A. Pishnamazi and M. J. Zuidhof. 2012. Altering Broiler Breeder Composition to Improve Support of Early Chick Quality and Long-term Maintenance of Lay. Final Report to Poultry Industry Council (Project #279) and Aviagen Inc. August, 2012. 32 pp.

21. Renema, R. A., Zuidhof, M. J., A. Pishnamazi, E. Tanjong-Mba, and T. G. V. Moraes. 2012. Restriction of broiler breeder body composition: A new management paradigm to support growth and yield Page | 50

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22.

23.

24.

25.

26.

potential of broiler offspring. Final Report to Alberta Livestock and Meat Agency and Alberta Innovates Bio-Solutions: Project 2009F130R. July, 2012. 32 pp.

Schneider, B. L., V. L. Carney, and D. Banks. 2012. We are one: flock uniformity and how to calculate it. Medium Egg Update December.

The PRC Annual Report (2011-2012). The PRC, The University of Alberta, Edmonton, AB, T6G 2P5. 50 pp.

Torres, C. A. and D. R. Korver. 2012. The influence of maternal age and dietary Cu, Zn and Mn on bone development of chicken embryos. Final project report to Novus International. 31 pages.

Torres, C. A. and D. R. Korver. 2012. The influence of maternal age and dietary Cu, Zn and Mn on bone development at hatch and post-hatch. Final project report to Novus International. 24 pages.

Zuidhof, M. J., F. E. Robinson, D. R. Korver, M. Betti, J. Wu, V. L. Carney, B. L. Schneider, E. Beltranena, E. Goddard, C. Bench, L. M. McMullen, and W. Wismer. PRC: Excellence in production, efficiency and product utilization. Final Report to Alberta Livestock and Meat Agency and Alberta Innovates Bio-Solutions: Project 2004L011P. November 7, 2012. 160 pp.

Trade articles 1. Zuidhof, M. J. 2012. Temperature and Protein: Energy ratio linkages between breeder and broiler energetics, carcass quality and performance. Canadian Poultry. May, 2012. http://www. agannex. com/production/temperature-and-protein.

2. Hernandez, F. I. L., and M. J. Zuidhof. 2012. Less does not mean more: Using less protein in broiler feed affects production. Canadian Poultry. August, 2012. http://www. agannex. com/sustainability/less-does-not-mean-more.

Patent Applications: 1. M. Betti, M. Ndagijimana. Enzymatic method for producing low molecular weight peptides (US provisional patent, filed on October 26, 2012 - application Serial No. 61/718,876) 2. M. Betti, M. Ndagijimana. Non enzymatic glycosylation of proteins and peptides in a liquid system at moderate temperatures (US provisional patent, filed on October 26, 2012 -application Serial No. 61/718,975) 3. M. Betti, M. Ndagijimana. Enzymatic method for glycosylation of proteins and peptides to enhance protein functionality, peptide activity and flavor (US provisional patent, filed on October 26, 2012 application Serial No. 61/718,968)

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Acronyms and Abbreviations Used AAFC ACP AFNS Ag AGM AHEP AI-Bio ALES ALMA ARD ATP BW CAAP CHEP EFA EFC FGSR FTE GCC GRAF GSA HQP MDLP MSc MSPRU NEP NSERC NSERC DG NSERC RT PhD Post doc PRC R&D RTE SAFUG SDA SHR U of A U/G VP VSCHT WPC

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Agriculture and Agri-food Canada Alberta Chicken Producers Department of Agricultural, Food and Nutritional Science Agriculture Annual general meeting Alberta Hatching Egg Producers Alberta Innovates Bio-Solutions Agricultural, Life and Environmental Sciences Alberta Livestock and Meat Agency Alberta Agriculture and Rural Development Alberta Turkey Producers Body weight Canadian Agricultural Adaptation Program Canadian Hatching Egg Producers Egg Farmers of Alberta Egg Farmers Canada Faculty of Graduate Studies and Research Full time equivalent Grand Challenges Canada Graduate Research Assistantship Fund Graduate Student Association Highly qualified personnel Market Development Leasing Program Master of Science Meat Safety/Processing Research Unit New Entrant Program (EFA) National Science and Engineering Research Council NSERC Discovery Grant NSERC Research Tools and Instruments Program Doctor of Philosophy Post doctoral fellow The PRC Research and development Ready to eat Sustainable Aviation Fuel Users Group Stearodonic Acid Spontaneously hypertensive rat University of Alberta undergraduate student Vice President Scholarship program (The Institute of Chemical Technology, Prague, CZ) World’s Poultry Congress

The PRC Annual Report 2013