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800.436.9910

info@peprotech.com www.peprotech.com

HEK293 Medium Maintenance Media for Human HEK293 Cells PeproGrow HEK293 • Animal component-free, serum-free, protein-free, chemically-defined medium • Complete medium containing L-alanyl-L-Glutamine for direct product use • High recombinant protein expression • Affordable and competitive pricing

POTENCY

PROLIFERATION

STABILITY


Phone: 800.436.9910 | 609.497.0253 Fax: 609.497.0321 info@peprotech.com sales@peprotech.com

5 Crescent Avenue P.O. Box 275 Rocky Hill, NJ 08553 United States

www.peprotech.com

PeproGrow HEK293 Medium PeproGrow HEK293 is an animal component-free, protein-free, serum-free, chemically-defined, complete medium formulation for the in vitro cultivation of HEK293 cells (Life TechnologiesTM FreeStyleTM 293-F cells, catalog number R790-07). This medium is intended for recombinant protein expression in suspension culture, which is recommended for a 5 day batch culture with a seeding density of 0.6 x106 cells/mL. This ready-to-use medium contains L-alanyl-L-Glutamine, amino acids, vitamins, and salts. An adaptation process is not required for Life TechnologiesTM FreeStyleTM 293-F cells. These graphs illustrate HEK293 BMP-6 expression in PeproGrow HEK293 medium and competitor CD-293 medium.

120.00

4.50 100.00

4.00

Percent Viability (%)

Viable Cell Density (x106 cells/mL)

5.00

3.50 3.00 2.50 2.00 PeproGrow AF-CD-HEK293

1.50 1.00

Competitor CD-293

0.50 0.00

0

1

2

3

4

5

80.00 60.00 40.00

PeproGrow AF-CD-HEK293

20.00

Competitor CD-293

0.00

6

0

1

2

3

Days

4

5

6

Days

18.00 16.00

Yield ( g/liter)

14.00 12.00 10.00 8.00 6.00

PeproGrow AF-CD-HEK293

4.00

Competitor CD-293

2.00 0.00

0

1

2

3

4

5

6

Days

PeproGrow HEK293 Media: PeproGrow HEK293

Catalog #AF-CD-HEK293

Usage: For in vitro use. Not for use in diagnostic or therapeutic procedures.

1L

$80

Hek293 media flyer