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BIOMEDICAL

Miniaturized Immunosensor Assembled from Colloidal Particles Between Micropatterned Electrodes Inventors:

Patent Number: Issue Date:

Kaler, Eric W.; Velev, Orlin D. 6,333,200 December 25, 2001

Docket Number: UD98-19

Immunological tests for specific diseases, pathogens, or allergens are routinely used in hospitals, laboratories, food, drug, and environmental control, and others, and are typically carried out by latex agglutination. This method involves optical reading of the turbidity of a dispersion. These methods constitute a multi-billion dollar industry, which is rapidly growing now because of increased demand for better health care and environmental quality. Our invention provides a general means to replace the agglutination assays with sensors that can test simultaneously for the presence of different molecules in very small samples. The invention is based on the use of standard micropatterned conductive electrodes connected to sensing elements. After the sensors are brought into contact with a solution containing the target molecules, a series of events leads to a signal that can be read directly by a computer. We have tested our idea experimentally and shown that the device works as expected. The extreme miniaturization of the active area leads to very high sensitivity, which can be estimated as being at least 200 times higher than the agglutination assays available today. The device is disposable and relatively inexpensive. The invention can also be applied to test for DNA fragments and other genetic markers.

SCIENCE

Bioactive Peptides for Cell Adhesion Inventors:

Serial Number: Filing Date:

Farach-Carson, Mary C.; Carson, Daniel D.; Safran, Jeffrey B. 10/363,376 July 7, 2003

Docket Number: UD01-01

The invention is directed to a polypeptide derived from domain IV of the extracellular matrix protein perlecan that can selectively adhere cells, nucleic acids encoding the inventive polypeptide, vectors comprising the nucleic acids, devices comprising a scaffold coated with the inventive polypeptide, and methods of adhering cells to a scaffold using the inventive polypeptide. Uptake of the Sperm Adhesion Molecule 1 (SPAM1 or PH-20) by Mammalian Sperm Via Its Lipid Anchor in an In Vitro System Inventor:

Serial Number: Filing Date:

DeLeon, Patricia A.; Chen, Hong; Zhang, Hong; Griffiths, Genevieve S. 60/528,312 December 9, 2003

Docket Number: UD04-18

This invention deals with the transfer of biologically active SPAM1 to mammalian spermatozoa via secretory vesicles in an in vitro environment. SPAM1 is the most widely conserved mammalian sperm membrane protein and plays multiple roles in fertilization. The best of these functions is its hyaluronidase activity that is necessary for the dissolution of the cumulus cells that surround and form a barrier around the oocytes. SPAM1 is secreted in vivo in vesicles in the epididymal epithelium and in vitro in the medium of cultured cells in both a soluble and a membrane-bound form, the latter being in the form of epididymosomes. Sperm exposed to SPAM1 via epididymosomes, obtained after ultracentrifugation of the epididymal luminal fluid or culture medium, or in the form of a recombinant protein with its lipid anchor intact, acquire the protein after co-incubation. Transfer of SPAM1 to the surface of the sperm is vesiclemediated and apparently similar to the epididymosome-spermatozoa interactions that occur in vivo during epididymal sperm maturation. 9

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